Name: Recombinant Human Transcriptional enhancer factor TEF-1 (TEAD1)
Brand: CUSABIO
SKU: CSB-EP023363HU

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

TEAD1

Uniprot No.

P28347

Research Area

others

Alternative Names

AA; Atrophia areata peripapillary chorioretinal degeneration; NTEF 1; NTEF-1; NTEF1; Protein GT IIC; Protein GT-IIC; REF 1; REF1; SV40 transcriptional enhancer factor; TCF 13; TCF-13; TCF13; TEA domain family member; TEA domain family member 1 (SV40 transcriptional enhancer factor); TEA domain family member 1; TEAD 1; TEAD 1 protein; TEAD-1; TEAD1; TEAD1 protein; TEAD1_HUMAN; TEF 1; TEF1; Transcription factor 13 (SV40 transcriptional enhancer factor); Transcription factor 13; Transcriptional enhancer factor 1; Transcriptional enhancer factor TEF-1; Transcriptional enhancer factor TEF1

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-426aa

Target Protein Sequence

MEPSSWSGSESPAENMERMSDSADKPIDNDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARRKSRDFHSKLKDQTAKDKALQHMAAMSSAQIVSATAIHNKLGLPGIPRPTFPGAPGFWPGMIQTGQPGSSQDVKPFVQQAYPIQPAVTAPIPGFEPASAPAPSVPAWQGRSIGTTKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLNCNIQDDAGAFYGVTSQYESSENMTVTCSTKVCSFGKQVVEKVETEYARFENGRFVYRINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVSNSEHGAQHHIYRLVKD
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

51.9 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The production of this recombinant Human TEAD1 protein is just like all recombinant proteins. The process involved transfecting E.coli cells with DNA vector containing the template of recombinant DNA. The E.coli cells containing the template were then cultured so that they could transcribe and translate the TEAD1 protein. N-terminal 6xHis tag was used in the process. The purity is 0.85 determined by SDS-PAGE.

Members of the highly related TEF-1 family (also known as TEAD, for TEF-1, TEC1, ABAA domain) bind to MCAT (muscle C, A and T sites) and A/T-rich sites in promoters active in cardiac, skeletal and smooth muscle, placenta, and neural crest. The gene regulatory activities of TEF-1 are governed by interactions with protein cofactors. For instance, interactions of TEF-1 with Max and serum response factor regulate the MHC gene and normal cardiac and smooth muscle development. TEF-1 family members are broadly expressed. But function as transcriptional activators only in a subset of their expression domains (cardiac, skeletal and smooth muscle, placenta, and skin. The strong transcriptional co-activator YAP65 interacts with all TEF-1 family members. TEAD transcription factors are key components of the Hippo–YAP1 signaling pathway. Some studies suggested that conclude that the mutation in the TEAD1 gene is the cause of Sveinsson's chorioretinal atrophy.

Customer Reviews and Q&A

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■ Q&A

For item CSB-EP023363HU lot# 03723 would you please send me the datasheet showing the buffer for this item.

Thanks for your inquiry. The storage buffer of this product is 20mM Tris-HCl, 0.5M NaCl, pH 8.0, 10% glycerol. The Datasheet&COA is attached for your reference.

I am writing in reference to protein product number CSB-EP023363HU. We ran MS2 on it and the profile looks very odd; possibly due to the presence of detergents (triton or NP40). This is an issue and we cannot run the HDX-MS experiment that we had planned. Please advise. Is it possible that there are traces of detergent in the product?

1. The detergent (triton or NP40) mentioned by you does not exist in our buffer.
2. Our protein was first refolded by TE, and TE buffer contains EDTA, and there may be traces of EDTA residue in dialysis buffer. Please confirm whether your result is caused by the influence of EDTA. If yes, we could resend 100ug of this protein WITHOUT EDTA free of charge. The lead time for the replacement will be 15-25 business days.
3. The MS we performed ourselves is gel point mass spectrometry identification, which is not the same as yours. In our MS identification, the buffer does not interfere with the identification.

Target Background

Function

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.

Gene References into Functions

YAP1 interacted with TEAD1, exerted their transcriptional control of the functional target, glucose transporter 1 (Glut1). PMID: 28892790
Yap1 post-translational modifications favoring its ubiquitination and apoptosis characterize hepatocellular carcinoma (HCC) with better prognosis, whereas conditions favoring the formation of YAP1-TEAD complexes are associated with aggressiveness and acquisition of stemness features by HCC cells PMID: 27359056
TEAD1 and TEAD4 are oncogenic factors, whose aberrant activation are, in part, mediated by the silence of miR-377-3p, miR-1343-3p and miR-4269. PMID: 28759040
adult human and mouse hearts had more Taz than Yap1 by mRNA and protein expression and their increases in diseased hearts were proportional and did not change Yap1/Taz ratio. Yap1, Taz, and Tead1 were accumulated in the nuclear fraction and cardiomyocyte nuclei of diseased hearts PMID: 29154888
Here, the authors show that TEAD1-expressing skeletal muscle of transgenic mice features a dramatic hyperplasia of muscle stem cells (i.e. satellite cells, SCs) but surprisingly without affecting muscle tissue size. PMID: 27725085
This identifies the YAP1/TEAD1 complex as the representative dysregulated profile of Hippo signaling in OS and provides proof-of-principle that targeting TEAD1 may be a therapeutic strategy of osteosarcoma. PMID: 28483529
The authors show MRTF family proteins bind YAP via a conserved PPXY motif that interacts with the YAP WW domain. This interaction allows MRTF to recruit NcoA3 to the TEAD-YAP transcriptional complex and potentiate its transcriptional activity. PMID: 28028053
MYC and TEAD activity is able to stratify different breast cancer subtypes in large panels of breast cancer patients. PMID: 27433809
Collectively, these results indicate that human papillomavirus 16 E6 induces upregulation of APOBEC3B through increased levels of TEADs, highlighting the importance of the TEAD-APOBEC3B axis in carcinogenesis. PMID: 28077648
Upregulation of transcriptional enhancer activator domain 1 was found in hepatocellular carcinoma tissues and inversely correlated with miR-590-3p. Our results indicate a tumor suppressor role of miR-590-3p in hepatocellular carcinoma through targeting transcriptional enhancer activator domain 1 and suggest its use in the diagnosis and prognosis of liver cancer. PMID: 28349829
TEAD1 mediates YAP1 chromatin-binding genome-wide. PMID: 26295846
show that the proangiogenic microfibrillar-associated protein 5 (MFAP5) is a direct transcriptional target of YAP/TEAD in cholangiocarcinoma cells transcription factors. PMID: 26173433
TAZ negatively regulate transcription of DeltaNp63 through TEAD1,2,3 and 4 transcription factors. PMID: 25995450
Our data suggest that AIC is a genetically heterogeneous disease and is not restricted to the X chromosome, and that TEAD1 mutations may be present in male patients. PMID: 26091538
Our findings suggest that genetic variants of Hippo pathway genes, particularly YAP1 rs11225163, TEAD1 rs7944031 and TEAD4 rs1990330, may independently or jointly modulate survival of CM patients. PMID: 25628125
Suggest central role for TEAD and YAP as signal-responsive regulators of multipotent pancreatic progenitors. PMID: 25915126
the YAP-TEAD interaction can be disrupted using cyclic YAP-like peptides, which targets the HIPPO pathway PMID: 25384421
the first evidence demonstrating that TEAD1 is a novel general repressor of smooth muscle-specific gene expression through interfering with myocardin binding to SRF. PMID: 24344135
identified an intronic region of the NAIP gene responding to TEAD1/YAP activity, suggesting that regulation of NAIP by TEAD1/YAP is at the transcriptional level PMID: 23994529
Data indicate that knockdown of TEAD1/3/4 induces an almost identical cellular senescent phenotype as YAP silencing. PMID: 23576552
our data reveal a new, Livin-dependent, apoptotic role for TEAD1 in mammals and provide mechanistic insight downstream of TEAD1 deregulation in cancers. PMID: 23029054
TEAD1 has non-AUG translation initiation PMID: 1851669
These results are consistent with two plausible models of cryptic MCAT enhancer regulation by Pur alpha, Pur beta, and MSY1 involving either competitive single-stranded DNA binding or masking of MCAT-bound transcription enhancer factor-1. PMID: 11751932
Transcription enhancer factor 1 binds multiple muscle MEF2 and A/T-rich elements during fast-to-slow skeletal muscle fiber type transitions PMID: 12861002
the mutation in the TEAD1 gene is the cause of Sveinsson's chorioretinal atrophy. PMID: 15016762
regulation of IFITM3 by TEF-1 demonstrates that TEF-1 dependent regulation is more widespread than its previously established role in the expression of muscle specific genes PMID: 18177740
TEAD1 inhibits prolactin gene expression in cultured human uterine decidual cells. PMID: 18775765
TEAD1 and the ubiquitin ligase c-Cbl were identified as novel basal cell markers in prostate cancer PMID: 19002168
Here we show that during vertebrate neural tube development, the TEA domain transcription factor (TEAD) is the cognate DNA-binding partner of YAP. PMID: 19015275
The primary cellular origin of circumpapillary dysgenesis of the pigment epithelium is within the choroid instead of the pigment epithelium. PMID: 19410955
A missense mutation (Y421H) in TEAD1 is tightly linked to Sveinsson's chorioretinal atrophy (SCRA), an autosomal dominant eye disease characterized by symmetrical lesions radiating from the optic disc involving the retina and the choroid. PMID: 15016762
TEAD1 (TEF-1) interacts with a muscle-specific cofactor to promote skeletal muscle gene expression. PMID: 12376544

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Involvement in disease

Sveinsson chorioretinal atrophy (SCRA)

Subcellular Location

Nucleus.

Tissue Specificity

Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.

Database Links

HGNC: 11714

OMIM: 108985

KEGG: hsa:7003

STRING: 9606.ENSP00000354588

UniGene: Hs.655331

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