Product Details

Purity

>85% (SDS-PAGE)

Target Names

Ddr2

Uniprot No.

Q62371

Alternative Names

Ddr2; Ntrkr3; Tkt; Tyro10Discoidin domain-containing receptor 2; Discoidin domain receptor 2; EC 2.7.10.1; CD167 antigen-like family member B; Neurotrophic tyrosine kinase; receptor-related 3; Receptor protein-tyrosine kinase TKT; Tyrosine-protein kinase TYRO10; CD antigen CD167b

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. Required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.

Gene References into Functions

DDR2 is important for maintenance of osteoblast activity and suppression of marrow adipogenesis in vivo and these actions are related to changes in MAPK-dependent RUNX2 and PPARgamma phosphorylation. PMID: 27341689
circulating fibroblast precursors expressing DDR2, in an exposure-induced model of pulmonary fibrosis, is reported. PMID: 28700752
This work identifies DDR2 as a potential therapeutic target that controls breast cancer metastases through its action in both tumor cells and tumor-stromal cells at the primary tumor site. PMID: 27264173
DDR2 mutation can drive lung cancer initiation in vivo and provide a novel mouse model for lung cancer therapeutics studies. PMID: 26206333
RESULTS Data show that DDR2 (discoidin domain receptor 2) suppresses osteoclast differentiation and activity. PMID: 25805889
DDR2 signaling regulates cell proliferation and extracellular matrix synthesis, which are key aspects of fibroblast contribution to tissue healing [review] PMID: 24781958
Germline deletion of the DDR2 results in smaller hearts, shorter cardiomyocytes, lower interstitial cardiac collagen density and abnormalities in cardiac function. PMID: 24993042
Taken together, our data demonstrated that DDR2 might play a systemic role in the regulation of body size thorough skeletal formation and fat metabolism. PMID: 24036888
Loss of DDR2 suppresses tumor angiogenesis and tumor metastasis to the lung. PMID: 24293323
DDR2 is involved in the collagen I-induced IL-12 production via NF-kappaB and JNK pathway PMID: 23583376
these data demonstrated that DDR2 might play a local and essential role in the proliferation of chondrocytes. PMID: 23022180
DDR2 deficiency predisposes hepatic tissue to colon carcinoma metastasis. DDR2 deficiency fosters myofibroblast transdifferentiation of tumour-activated hepatic stellate cells (HSCs), generating a prometastatic microenvironment in the liver. PMID: 22071959
DDR2 regulated the transactivity of Runx2, a master transcription factor involved in skeletal development, by modulating its phosphorylation. PMID: 20734453
Reduction of DDR-2 expression attenuates the articular cartilage degeneration of knee joints induced either by type XI collagen deficiency or by surgical destabilization of the medial meniscus. PMID: 20518074
Study results show that Ddr2 significantly reduced the persistence length and Young's Modulus of collagen type I fibers. PMID: 20346496
since soluble forms of DDR1 and DDR2 containing its ECD are known to naturally exist in the extracellular matrix, in this work we investigated if these soluble DDR ECDs may have a functional role in modulating collagen fibrillogenesis PMID: 19900459
Results indicate that discoidin domain receptor 2 signaling plays a critical role in the maintenance of male spermatogenesis. PMID: 19681157
Binding of DDR2 complexes onto individual molecules of triple-helical type I collagen has been characterized by an atomic force microscopy study, providing insight into the number and locations of binding sites on collagen I. PMID: 12220173
the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation PMID: 16806867
These results demonstrate a novel and important functional role of the DDR2 extracellular domain that may contribute to collagen regulation via modulation of fibrillogenesis. PMID: 16884738
DDR2 is a critical collagen receptor for dendritic cells (DCs) activation and that DDR2-collagen interaction plays an important role in the functional capacity of DCs regulating immune responses PMID: 17113033
DDR2 is expressed in adipose cells and that its overexpression leads to insulin resistance. PMID: 17712722
These results indicate that the absence of DDR2 leads to growth retardation and gonadal dysfunction due to peripheral defects in hormonal-responsive pathways in slie mice. PMID: 18483174
observations extend the functional roles that DDR2 and possibly other membrane-anchored, collagen-binding proteins can play in the regulation of cell adhesion, migration, proliferation and in the remodeling of the extracellular matrix PMID: 18996394

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Involvement in disease

Defects in Ddr2 are the cause of the smallie (sli) phenotype. Smallie mice show distinct dwarfing, with reduced body mass and reduced bone mineral content. Mice also have mild craniofacial deformities, such as protuberant eyes and snub noses. Smallie mice have a reduced life span, with about half of them dying within 6 months. Matings between male and female smallie mice do not yield any offspring. The levels of circulating steroid hormones remain at a level corresponding to prepubertal wild-type mice. Adult testes exhibit much reduced numbers of spermatids with atrophy of spermatogonia, Sertoli and Leydig cells. Ovaries show an absence of corpora lutea.

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily

Tissue Specificity

Widely expressed. Detected in lung, ovary, skin and in testis Leydig cells (at protein level). Widely expressed. Detected at high levels in heart, lung, skeletal muscle, central nervous system (CNS) and kidney, and at lower levels in brain and testis. Det

Database Links

KEGG: mmu:18214

STRING: 10090.ENSMUSP00000027985

UniGene: Mm.229249

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Recombinant Mouse Discoidin domain-containing receptor 2 (Ddr2), partial

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Target Background

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