Recombinant Mouse Neuropilin-1(Nrp1) ,partial

Code CSB-YP016091MO
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Source Yeast
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Code CSB-EP016091MO
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Source E.coli
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Code CSB-EP016091MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP016091MO
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Source Baculovirus
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Code CSB-MP016091MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Nrp1
Uniprot No. P97333
Alternative Names Nrp1; Nrp; Neuropilin-1; A5 protein; CD antigen CD304
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins. Binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis (By similarity). Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development
Gene References into Functions
  1. Our findings suggest a pivotal role for adipose tissue-resident NRP1(+)-expressing macrophages in driving healthy weight gain and maintaining glucose tolerance. PMID: 29549139
  2. ErbB2 interacts with Nrp1 to form a Sema3d co-receptor expressed by developing coronary endothelium and required for proper connections of the forming coronary veins to the right atrium. PMID: 27356767
  3. inducible endothelial genetic deletion of Neuropilin1 (Nrp1) and Vascular endothelial growth factor receptor 1 (Vegfr1; also known as Flt1) renders mice resistant to diet-induced obesity. PMID: 30093598
  4. a novel role for Sema3A-neuropilin 1 signalling in progenitor cell dynamics and in the generation of interneurons in the ventral telencephalon PMID: 27858201
  5. Tuftsin prevents the negative immunoregulation of neuropilin-1highCD4+CD25+Regulatory T cells and improves survival rate in septic mice. PMID: 27835904
  6. the induction of NRP-1 on macrophages may be a therapeutic indicator for antibody treatments that exert antibody-dependent cellular cytotoxicity activity. PMID: 28432397
  7. Nrp1 mediates the migratory behavior of mesangial cells. PMID: 28835419
  8. These data suggest that both Nrp1 and Sema3a are expressed in the cochlea during time points when spiral ganglion cell begin to innervate the organ of Corti. PMID: 29059194
  9. Reveal a postdevelopmental role of Nrp1 in renal pericytes as an important regulator of glomerular basement membrane integrity. PMID: 27988210
  10. Conditional Nrp1 knockout study does not support the Nrp1 model - that olfactory-derived cAMP signals, rather than direct action of olfactory receptor molecules, determine the target destinations (glomeruli) of olfactory sensory neurons in the olfactory bulb - for the anterior-posterior patterning of glomerular positions in the olfactory bulb. PMID: 27844052
  11. It was concluded that the HFD-instigated Nrp1 reduction in macrophages exacerbates insulin resistance by promoting Nlrp3 inflammasome priming and activation. PMID: 28659345
  12. findings identify a dual function of NRP1 in both axon guidance and subcellular target recognition in the construction of GABAergic circuitry. PMID: 27618676
  13. Ectopic midline vascularisation in endothelial Nrp1 and Vegfa(188/188) mutants caused additional axonal exclusion zones within the chiasm. PMID: 28676569
  14. Data suggest that Nrp1 (neuropilin-1) regulates Hedgehog signaling specifically at the level of activation of GLI2 transcriptional activator function; Nrp1 localization to primary cilium does not correlate with Hedgehog signal promotion. These studies were conducted in various cell types. (GLI2 = GLI-Kruppel family member GLI2 protein) PMID: 28667171
  15. These studies reported that myeloid cell-specific Nrp1-deficient mice exhibited enhanced susceptibility to cecal ligation and puncture- and LPS-induced sepsis, which correlated with significantly decreased survival rates and heightened levels of proinflammatory cytokines in both peritoneal lavage and serum. PMID: 28325756
  16. VEGF165-induced vascular leakage requires both VEGFR2 and NRP1, including the VEGF164-binding site of NRP1 and the NRP1 cytoplasmic domain (NCD), but not the known NCD interactor GIPC1. PMID: 28289053
  17. demonstrate that NRP-1 is necessary for VEGF to inhibit bone marrow-derived DC (BMDC) maturation; further demonstrate that NRP-1 associates with TLR4, but not VEGFR2, in a VEGF-dependent manner, and abrogates TLR signaling; results reveal a novel role for NRP-1 in DC physiology and identify NRP-1 as a target to manipulate BMDC maturation PMID: 27815442
  18. The aim was to elucidate whether ectopic muscles are a result of misguidance of myoblast precursors due to the loss of Sema3A-Npn-1 signaling. PMID: 27466379
  19. These data identify a new molecular mechanism of brain microvascular endothelial inflammatory response through NRP1-IFNgamma crosstalk. PMID: 27591257
  20. that microglial expression of Neuropilin-1 (Nrp1) is required for tuftsin-driven amelioration of EAE symptoms PMID: 26880314
  21. Sepsis per se markedly promoted the expression of Nrp-1 of CD4(+)CD25(+)Tregs. Foxp-3/CTLA-4/TGF-beta(m+) of Nrp-1(high)Tregs were upregulated by septic challenge. PMID: 27239104
  22. Both placental NRP1 and VEGF were expressed at lower levels in women with pre-eclampsia and homocysteine-treated mice, which may contribute to endothelial damage. PMID: 26708340
  23. The results show that high vs. low expression of Nrp-1 or Helios does not unequivocally identify Treg clones of thymic or peripheral origin. PMID: 26495986
  24. Nrp1 in endothelial cells suppresses TGFbeta activation and signaling by forming intercellular protein complexes with beta8 integrin. PMID: 26586223
  25. In vascular sprouting, neuropilin-1 suppresses the stalk-cell phenotype by limiting Smad2/3 activation through Alk1 and Alk5. Notch downregulates Nrp1, thus relieving the inhibition of Alk1 and Alk5, thereby driving stalk-cell behaviour. PMID: 26081042
  26. NRP1 enables ECM-induced filopodia formation for tip cell function during sprouting angiogenesis. PMID: 26051942
  27. demonstrate that in mice on the C57Bl/6 background EMX1 plays an essential role in the midline crossing of an axonal subpopulation of the corpus callosum derived from the anterior cingulate cortex. In the absence of EMX1, cingulate axons display reduced expression of the axon guidance receptor NRP1 and form aberrant axonal bundles within the rostral corpus callosum. PMID: 26534986
  28. Study showed that Nrp1 expression paralleled with that of DDR2 during osteoblast differentiation. Nrp1 assisted the promoting role of DDR2 in osteoblast differentiation, via activation of DDR2-mediated downstream signaling. PMID: 25924845
  29. These findings indicate a new role for Nrp1 in the maintenance of the visceral SMC contractile phenotype required for normal GI motility in aged mice. PMID: 25659123
  30. These data suggest that graft rejection occurs, at least in part, through the loss of Nrp1 expression on regulatory CD4(+) T cells, their stability or both. PMID: 25245111
  31. The results of the present study suggest that some soluble factors from smooth muscle cells may affect the localization of Nrp-1 in cholinergic neuronal cells, which may, in turn, be involved in the autonomic innervation of blood vessels. PMID: 25416424
  32. LPA5 might be involved in both nociception and mechanisms of pain hypersensitivity, as well as in anxiety-related and motivational behaviors. These observations further support the proposed involvement of LPA signaling in psychopathology. PMID: 23039190
  33. Sox2 is required for cutaneous squamous cell carcinoma growth in mouse and human, where it enhances Nrp1/Vegf signaling to promote the expansion of tumor initiating cells along the tumor-stroma interface. PMID: 25077433
  34. analyzed a complementary set of ligand-specific and tissue-specific mutants to show that neural crest-derived SEMA3C activates NRP1 in the cardiac outflow tract endothelium PMID: 26053665
  35. expression of Neuropilin1 (Npr1) and Slit2 mRNA was diminished in Islet mutant mice PMID: 25843547
  36. Nrp1 had no detectable role in the tolerant phenotype. PMID: 25343644
  37. These results indicate that NP1 negatively regulates excitatory synapse number by modulating neuronal excitability and show that NP1 restricts excitatory synaptic plasticity. PMID: 25855168
  38. Data (including data from mouse mutant strains) suggest Nrp1 in vascular endothelium contributes to angiogenesis via VEGFA-dependent and VEGFA-independent mechanisms; the latter involves Abl1 (c-abl proto-oncogene 1) and extracellular matrix. [REVIEW] PMID: 25233427
  39. Data suggest that loss of semaphorin-3A (Sema3A)-neuropilin 1 (Npn1) signaling is not the only cause for the developmental deficits. PMID: 25512301
  40. We cloned a novel mouse sNrp1 isoform from liver and provide evidence that this endogenous angiogenesis inhibitor may regulate VEGF or HGF bioavailability during normal physiological growth and development as well as during liver regeneration. PMID: 24977735
  41. Data indicate that ABL1 forms a complex with neuropilin 1 (NRP1) in endothelial cells (ECs). PMID: 24863063
  42. This study examines the hypothesis that Sema3A signaling through its motor neuron neuropilin-1 (NRP1) receptor triggers distal axonopathy PMID: 24918638
  43. Nrp1 is expressed in lymphatic valves and interacts with semaphorin 3A in lymphangiogenesis [review] PMID: 24276892
  44. Despite the loss of VEGF binding and attenuated NRP1 expression, homozygous Nrp1(Y297A/Y297A) mice were born at normal Mendelian ratios, arguing against NRP1 functioning exclusively as a VEGF164 receptor in embryonic angiogenesis. PMID: 24401374
  45. Conditional Nrp1 deletion beginning at postnatal day 5. PMID: 23713442
  46. shows that TAMs' heterogeneity depends on their localization, which is tightly controlled by Sema3A/Nrp1 signaling PMID: 24332039
  47. tuftsin-mediated anti-inflammatory M2 shift in microglia is caused specifically by tuftsin binding to the receptor neuropilin-1 PMID: 24033337
  48. Treg-cell stability can be subverted in certain inflammatory sites, but is maintained by a Sema4a-Nrp1 axis-- this pathway is a potential therapeutic target that could limit Treg-cell-mediated tumour-induced tolerance without inducing autoimmunity PMID: 23913274
  49. Loss of NRP1 expression is associated with impaired developmental arteriogenesis. PMID: 23639442
  50. NRP1 expression in keratinocytes is dispensable for normal skin development. Upon UVB challenge, NRP1 contributes to the prevention of keratinocyte apoptosis. PMID: 23251405

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Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Neuropilin family
Tissue Specificity Nervous system.
Database Links

KEGG: mmu:18186

STRING: 10090.ENSMUSP00000026917

UniGene: Mm.271745

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