Code | CSB-RA004996A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
IP | 1:200-1:1000 |
The production of the CDC25C recombinant monoclonal antibody involves the use of DNA recombinant technology and in vitro genetic manipulation. Initially, animals are immunized with a synthesized peptide derived from human CDC25C, leading to the isolation of B cells. Positive B cells are then selected, followed by screening and identification of individual clones. PCR amplification is employed to amplify the light and heavy chains of the CDC25C antibody, which are subsequently inserted into a plasmid vector. This recombinant vector is transfected into a host cell line to facilitate the expression of the antibody. The CDC25C recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. This antibody exhibits specificity towards human CDC25C protein and is recommended for five applications, including ELISA, WB, IHC, IF, and IP.
CDC25C is a protein phosphatase that plays a crucial role in the regulation of the cell cycle. Specifically, it activates CDK1, which is required for the G2/M transition. CDC25C dephosphorylates CDK1 at tyrosine 15, allowing it to become fully active and initiate mitosis. Additionally, CDC25C is regulated by a number of upstream signaling pathways, including the checkpoint kinase 1 (CHK1) and checkpoint kinase 2 (CHK2) pathways, which activate CDC25C in response to DNA damage or other stressors. Its activity is tightly controlled to ensure proper cell division and prevent the development of cancer and other diseases.
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