CDC25C Antibody

Datasheet
Code CSB-RA004996A0HU
Size US$350
Uniprot No. P30307
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate
    All lanes: Cdc25C antibody at 1.65μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 54, 52, 49, 46 KDa
    Observed band size: 60 KDa

  • IHC image of CSB-RA004996A0HU diluted at 1:165 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-RA004996A0HU at 1:55, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

  • Immunoprecipitating CDC25C in HEK293 whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-RA004996A0HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-RA004996A0HU (3μg) + HEK293 whole cell lysate (500μg)
    Lane 3: HEK293 whole cell lysate (20μg)

Immunogen A synthesized peptide derived from human CDC25C
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000
Relevance Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Research Area Cell Biology
Gene Names CDC25C
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Subcellular Location Nucleus
Protein Families MPI phosphatase family
Database Links

HGNC: 1727

OMIM: 157680

KEGG: hsa:995

STRING: 9606.ENSP00000321656

UniGene: Hs.656

Pathway Cell cycle

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