Phospho-EIF4E (S209) Antibody

^Datasheet

Specification
Target

Code	CSB-RA007556A209phHU
Size	US$350
Uniprot No.	P06730
Image	Western Blot Positive WB detected in：293 whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or not) All lanes：Phospho-EIF4E antibody at 1.2μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25 KDa Observed band size: 25 KDa IHC image of CSB-RA007556A209phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunogen	A synthesized peptide derived from Human Phospho-EIF4E (S209)
Species Reactivity	Human
Tested Applications	ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200
Relevance	Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Form	Liquid
Conjugate	Non-conjugated
Storage Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method	Affinity-chromatography
Isotype	Rabbit IgG
Clonality	Monoclonal
Alias	Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F
Immunogen Species	Homo sapiens (Human)
Protocols	ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol
Research Area	Epigenetics and Nuclear Signaling
Gene Names	EIF4E
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function	Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Involvement in disease	Autism 19 (AUTS19)
Subcellular Location	Cytoplasm, P-body, Cytoplasm
Protein Families	Eukaryotic initiation factor 4E family
Database Links	HGNC: 3287 OMIM: 133440 KEGG: hsa:1977 UniGene: Hs.13211
Pathway	HIF-1 signaling pathway mTOR signaling pathway PI3K-Akt signaling pathway Insulin signaling pathway

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Code	Product Name	Species Reactivity	Application
CSB-PA007556GA01HU	EIF4E Antibody	Human,Mouse,Rat	ELISA,WB,IHC,IF
CSB-PA935753	Phospho-EIF4E (Ser209) Antibody	Human,Mouse,Rat	ELISA,WB,IHC,IF
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EIF4E Antibodies for Human

Code	Product Name	Species Reactivity	Application
CSB-PA007556LA01HU	EIF4E Antibody	Human	ELISA, IHC, IF
CSB-PA007556LB01HU	EIF4E Antibody, HRP conjugated	Human	ELISA
CSB-PA007556LC01HU	EIF4E Antibody, FITC conjugated	Human	ELISA
CSB-PA007556LD01HU	EIF4E Antibody, Biotin conjugated	Human	ELISA

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EIF4E Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP007556HU CSB-EP007556HU CSB-BP007556HU CSB-MP007556HU	Recombinant Human Eukaryotic translation initiation factor 4E(EIF4E)	Yeast E.coli Baculovirus Mammalian cell

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Phospho-EIF4E (S209) Antibody

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