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Phospho-EIF4E (S209) Antibody

^Datasheet

Specification
Target

Code	CSB-RA007556A209phHU
Size	US$350
Uniprot No.	P06730
Abbreviation	Eukaryotic translation initiation factor 4E
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image	Western Blot Positive WB detected in：293 whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or not) All lanes：Phospho-EIF4E antibody at 1.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25 KDa Observed band size: 25 KDa IHC image of CSB-RA007556A209phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunogen	A synthesized peptide derived from human Phospho-EIF4E (S209)
Species Reactivity	Human
Tested Applications	ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200
Relevance	Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Form	Liquid
Conjugate	Non-conjugated
Storage Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method	Affinity-chromatography
Isotype	Rabbit IgG
Clonality	Monoclonal
Alias	Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F
Immunogen Species	Homo sapiens (Human)
Protocols	ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol
Research Area	Epigenetics and Nuclear Signaling
Gene Names	EIF4E
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Function	Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Involvement in disease	Autism 19 (AUTS19)
Subcellular location	Cytoplasm, P-body, Cytoplasm
Pathway	HIF-1 signaling pathway mTOR signaling pathway PI3K-Akt signaling pathway Insulin signaling pathway

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EIF4E Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA007556GA01HU	EIF4E Antibody	Human,Mouse,Rat	ELISA,WB,IHC,IF
CSB-PA935753	Phospho-EIF4E (Ser209) Antibody	Human,Mouse,Rat	ELISA,WB,IHC,IF
CSB-PA190338	EIF4E (Ab-209) Antibody	Human,Mouse,Rat	ELISA,WB,IHC,IF
CSB-PA000519	Phospho-EIF4E (S209) Antibody	Human,Mouse,Rat	WB,IHC,ELISA
CSB-PA002308	EIF4E Antibody	Human,Mouse,Rat,Monkey	WB,IHC,ELISA
CSB-PA007556LA01HU	EIF4E Antibody	Human	ELISA, IHC, IF
CSB-PA007556LB01HU	EIF4E Antibody, HRP conjugated	Human	ELISA
CSB-PA007556LC01HU	EIF4E Antibody, FITC conjugated	Human	ELISA
CSB-PA007556LD01HU	EIF4E Antibody, Biotin conjugated	Human	ELISA

EIF4E Proteins

EIF4E Proteins for Oryctolagus cuniculus (Rabbit)

Code	Product Name	Source
CSB-YP007556RB	Recombinant Rabbit Eukaryotic translation initiation factor 4E(EIF4E),Yeast	Yeast
CSB-EP007556RB	Recombinant Rabbit Eukaryotic translation initiation factor 4E(EIF4E),Escherichia Coli	E.coli
CSB-BP007556RB	Recombinant Rabbit Eukaryotic translation initiation factor 4E(EIF4E),Baculovirus	Baculovirus
CSB-MP007556RB	Recombinant Rabbit Eukaryotic translation initiation factor 4E(EIF4E),Mammalian cell	Mammalian cell

EIF4E Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP007556MO	Recombinant Mouse Eukaryotic translation initiation factor 4E(Eif4e),Yeast	Yeast
CSB-EP007556MO	Recombinant Mouse Eukaryotic translation initiation factor 4E(Eif4e),Escherichia Coli	E.coli
CSB-BP007556MO	Recombinant Mouse Eukaryotic translation initiation factor 4E(Eif4e),Baculovirus	Baculovirus
CSB-MP007556MO	Recombinant Mouse Eukaryotic translation initiation factor 4E(Eif4e),Mammalian cell	Mammalian cell

EIF4E Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP007556HU	Recombinant Human Eukaryotic translation initiation factor 4E(EIF4E),Yeast	Yeast
CSB-EP007556HU	Recombinant Human Eukaryotic translation initiation factor 4E(EIF4E),Escherichia Coli	E.coli
CSB-BP007556HU	Recombinant Human Eukaryotic translation initiation factor 4E(EIF4E),Baculovirus	Baculovirus
CSB-MP007556HU	Recombinant Human Eukaryotic translation initiation factor 4E(EIF4E),Mammalian cell	Mammalian cell

EIF4E Proteins for Xenopus laevis (African clawed frog)

Code	Product Name	Source
CSB-YP007556XBE	Recombinant Xenopus laevis Eukaryotic translation initiation factor 4E(eif4e),Yeast	Yeast
CSB-EP007556XBE	Recombinant Xenopus laevis Eukaryotic translation initiation factor 4E(eif4e),Escherichia Coli	E.coli
CSB-BP007556XBE	Recombinant Xenopus laevis Eukaryotic translation initiation factor 4E(eif4e),Baculovirus	Baculovirus
CSB-MP007556XBE	Recombinant Xenopus laevis Eukaryotic translation initiation factor 4E(eif4e),Mammalian cell	Mammalian cell

EIF4E Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP007556RA	Recombinant Rat Eukaryotic translation initiation factor 4E(Eif4e),Yeast	Yeast
CSB-EP007556RA	Recombinant Rat Eukaryotic translation initiation factor 4E(Eif4e),Escherichia Coli	E.coli
CSB-BP007556RA	Recombinant Rat Eukaryotic translation initiation factor 4E(Eif4e),Baculovirus	Baculovirus
CSB-MP007556RA	Recombinant Rat Eukaryotic translation initiation factor 4E(Eif4e),Mammalian cell	Mammalian cell

EIF4E Proteins for Dictyostelium discoideum (Slime mold)

Code	Product Name	Source
CSB-YP007556DKK	Recombinant Dictyostelium discoideum Eukaryotic translation initiation factor 4E(eif4e),Yeast	Yeast
CSB-EP007556DKK	Recombinant Dictyostelium discoideum Eukaryotic translation initiation factor 4E(eif4e),Escherichia Coli	E.coli
CSB-BP007556DKK	Recombinant Dictyostelium discoideum Eukaryotic translation initiation factor 4E(eif4e),Baculovirus	Baculovirus
CSB-MP007556DKK	Recombinant Dictyostelium discoideum Eukaryotic translation initiation factor 4E(eif4e),Mammalian cell	Mammalian cell

EIF4E Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP007556BO	Recombinant Bovine Eukaryotic translation initiation factor 4E(EIF4E),Yeast	Yeast
CSB-EP007556BO	Recombinant Bovine Eukaryotic translation initiation factor 4E(EIF4E),Escherichia Coli	E.coli
CSB-BP007556BO	Recombinant Bovine Eukaryotic translation initiation factor 4E(EIF4E),Baculovirus	Baculovirus
CSB-MP007556BO	Recombinant Bovine Eukaryotic translation initiation factor 4E(EIF4E),Mammalian cell	Mammalian cell

EIF4E cDNA

EIF4E cDNA for Oryctolagus cuniculus (Rabbit)

Code	Product Name	Vector	Accession NO.
CSB-CL007556RB	EIF4E	pUC	NM_001101710.1

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