Code | CSB-RA980100A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:2000 |
The generation of the STK3 recombinant monoclonal antibody involves a stringent process to ensure its exceptional quality and specificity. It begins by isolating B cells from an immunized animal, where the synthesized peptide derived from human STK3 serves as the immunogen. Total RNA is then extracted from these B cells and converted into cDNA through reverse transcription. Using specific primers designed for the antibody constant regions, the STK3 antibody genes are amplified and inserted into an expression vector. Through transfection, the vector is introduced into host cells, allowing for the production of the STK3 recombinant monoclonal antibody. After cell culture, the antibody is harvested from the supernatant and purified using affinity chromatography, resulting in a highly purified form suitable for various applications. Extensive characterization assays, such as ELISA and WB analysis, are conducted to validate the antibody's specificity and functionality in detecting human STK3 protein.
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