Recombinant Mouse Calcium uniporter protein, mitochondrial(Mcu)-VLPs

Code CSB-MP668882MO
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Product Details

Target Names Mcu
Uniprot No. Q3UMR5
Research Area Cardiovascular
Alternative Names Mcu; Calcium uniporter protein, mitochondrial
Species Mus musculus (Mouse)
Source Mammalian cell
Expression Region 50-350aa
Target Protein Sequence AHQRPASWQSVGAAYCSTVVPSDDVTVVYQNGLPVISVRLPSRRERCQFTLKPISDSVGVFLRQLQEEDRGIDRVAIYSPDGVRVAASTGIDLLLLDDFKLVINDLTYHVRPPKRDLLSHEDAATLNDVKTLVQQLYTTLCIEQHQLNKERELVERLEDLKQQLAPLEKVRIEISRKAEKRTTLVLWGGLAYMATQFGILARLTWWEYSWDIMEPVTYFITYGSAMAMYAYFVMTRQEYVYPEARDRQYLLFFHKGAKKSRFDLEKYNQLKDAIAQAEMDLKRLRDPLQVHLPLRQIGEKE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info C-terminal 10xHis-tagged
If you have specified tag type, please tell us and we will check if it’s possible to develop.
Form Lyophilized powder
Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.
Buffer Lyophilized from PBS, 6% Trehalose, pH 7.4
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store the protein at -20°C/-80°C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex). Activity is regulated by MICU1 and MICU2. At low Ca(2+) levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca(2+) levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells. Participates in mitochondrial permeability transition during ischemia-reperfusion injury. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults. Regulates synaptic vesicle endocytosis kinetics in central nerve terminal. Involved in antigen processing and presentation.
Gene References into Functions
  1. the endothelial barrier was preserved in respiratory epithelium isolated from MCU-/- mice after exposure to IL-13. In the ovalbumin-model of allergic airway disease, MCU deficiency resulted in decreased apoptosis within the large airway epithelial cells. Concordantly, expression of the tight junction protein ZO-1 was preserved, indicative of maintenance of epithelial barrier function PMID: 29225050
  2. Results suggest that inhibition of MCU attenuates Abeta-induced mcroglial apoptosis, and that it does so through modulation of reactive oxygen species-mediated endoplasmic reticulum stress. Understanding the apparent links between endoplasmic reticulum stress, oxidative stress and MCU could lead to the development of therapeutic strategies targeting Abeta-mediated microglial death. PMID: 28939404
  3. These data indicate that Ca(2+) uptake via mitochondrial uniporter contributes to palmitic acid-induced apoptosis in mouse podocytes. PMID: 28181698
  4. MCU expression returned to physiological levels in visceral adipose tissue of patients after weight loss by bariatric surgery. Altered mitochondrial calcium flux in fat cells may play a role in obesity and diabetes and may be associated with the differential metabolic profiles of visceral and subcutaneous adipose tissue. PMID: 28790027
  5. Regulation of mitochondrial Ca(2+) suggests that MCU may play a pivotal role in the development of fibrosis and could potentially be a therapeutic target for pulmonary fibrosis. PMID: 28351840
  6. Reveal a distinct functional role for Cys-97 in mitochondrial reactive oxygen species sensing and regulation of MCU activity. PMID: 28262504
  7. The m-AAA protease associated with neurodegeneration limits MCU activity in mitochondria. PMID: 27642048
  8. Data show that the serine/threonine kinase LKB1 regulates mitochondrial calcium uniporter (MCU)-expression, mitochondria-dependent Ca2+ clearance, and thereby, presynaptic release properties. PMID: 27429220
  9. the Mcu-regulating Micu gene family profile differs substantially between neurons and astrocytes, while expression of Mcu itself is markedly different between CA3 and CA1 regions in the adult hippocampus. PMID: 26828201
  10. Absence of MCU expression does not affect basal cardiac function at either 12 or 20months of age in knockout mice. PMID: 26057074
  11. MCU is required to match energetics with contractile demand during stress. Deletion of Mcu protects against myocardial IR injury. PMID: 26119731
  12. Mice lacking MCU in the heart show no pathology. MCU selectively mediates acute mitochondria Ca2+ loading to augment ATP synthesis. PMID: 26119742
  13. MCU is necessary for complete physiological heart rate acceleration PMID: 25603276
  14. MCU-VDAC1 complex regulates mitochondrial Ca(2+) uptake and oxidative stress-induced apoptosis PMID: 25753332
  15. Chromatin immunoprecipitation and promoter reporter analyses revealed that the Ca2+ -regulated transcription factor CREB (cyclic adenosine monophosphate response element-binding protein) directly bound the MCU promoter and stimulated expression. PMID: 25737585
  16. chronic myocardial MCU inhibition leads to previously unanticipated compensatory changes. PMID: 26153425
  17. Tetrodotoxin-sensitive mitochondrial Ca(2+) influx was largely blocked by knockdown of MCU expression. PMID: 24719357
  18. Characterization of MCU deficient mice indicates that acute alterations in mitochondrial matrix calcium can regulate mammalian physiology. PMID: 24212091
  19. Exogenously expressed Mcu is mitochondrially localized and increases mitochondrial Ca(2+) levels following NMDA receptor activation, leading to increased mitochondrial membrane depolarization and excitotoxic cell death. PMID: 23774321
  20. The mitochondrial calcium uniporter is a multimer that can include a dominant-negative pore-forming subunit. MCU has the fine control of the mitochondrial calcium homeostasis. PMID: 23900286
  21. Data show that the recently-identified mitochondrial Ca(2+) uniporter, MCU, is required for depolarisation-induced mitochondrial Ca(2+) increases, and for a sustained increase in cytosolic ATP/ADP ratio. PMID: 22829870
  22. genomic, physiological, biochemical and pharmacological data firmly establish MCU as an essential component of the mitochondrial Ca(2+) uniporter PMID: 21685886
  23. data demonstrate that the 40-kDa protein identified is the channel responsible for ruthenium-red-sensitive mitochondrial Ca(2+) uptake, thus providing a molecular basis for this process of utmost physiological and pathological relevance PMID: 21685888
  24. Includes expression analysis of the mouse Ccdc109a (MCU) gene. PMID: 21685888
  25. In vivo silencing of CCDC109a (MCU) in mouse liver abolishes mitochondrial calcium uptake without perturbing gross liver anatomy, mitochondrial oxygen consumption or membrane potential. PMID: 21685886
  26. Ccdc109a was identified in this large-scale proteomics analysis as a mitochondrial protein broadly expressed in many mouse tissues. The human protein was also confirmed to localize to mitochondria. PMID: 18614015

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Subcellular Location Mitochondrion inner membrane; Multi-pass membrane protein.
Protein Families MCU (TC 1.A.77) family
Tissue Specificity Detected in heart muscle (at protein level). Expressed in skeletal muscle, heart, kidney, liver, brain, lung, white fat and spleen.
Database Links

KEGG: mmu:215999

STRING: 10090.ENSMUSP00000020312

UniGene: Mm.296470

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