Recombinant Rat Carnitine O-palmitoyltransferase 1, liver isoform(Cpt1a)

Code CSB-CF005922RA
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names Cpt1a
Uniprot No. P32198
Alternative Names Cpt1a; Cpt-1; Cpt1; Carnitine O-palmitoyltransferase 1, liver isoform; CPT1-L; Carnitine O-palmitoyltransferase I, liver isoform; CPT I; CPTI-L; Carnitine palmitoyltransferase 1A
Species Rattus norvegicus (Rat)
Expression Region 2-773
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Plays an important role in hepatic triglyceride metabolism.
Gene References into Functions
  1. Obesity-alleviating potential of asiatic acid and its effects on ACC1, UCP2, and CPT1 mRNA expression in high fat diet-induced obese Sprague-Dawley rats PMID: 28993954
  2. Furthermore, given the already low abundance of Cpt1b in white adipose tissue, it is unlikely that decreases in its expression can quantitatively decrease whole body energy expenditure enough to contribute to an obese phenotype. PMID: 28330968
  3. e found that CPT1AM-expressing rBA have increased FAO, lipolysis, UCP1 protein levels and mitochondrial activity. Additionally, enhanced FAO reduced the palmitate-induced increase in triglyceride content and the expression of obese and inflammatory markers. Thus, CPT1AM-expressing rBA had enhanced fat-burning capacity and improved lipid-induced derangements. PMID: 27438137
  4. upregulation CPT1 expression leading to reduction in the accumulation of lipid intermediates in skeletal muscle. PMID: 23815800
  5. CPT-1 activity was altered in concert with fasting and refeeding states, supporting a physiological role of CPT-1a in mediating the control of feeding PMID: 23736540
  6. The implications are that L-CPT1 expression induces metabolic remodeling hypertrophic signaling and that regulatory factors beyond malonyl CoA in the heart regulate long chain fatty acid oxidation via L-CPT1. PMID: 22982985
  7. The hypolipidemic effect produced by donkey's milk paralleled with the enhanced mitochondrial activity/proton leakage and with the increased activity or expression of mitochondrial markers namely, carnitine palmitoyl transferase and uncoupling protein 2. PMID: 22930490
  8. -L-carnitine supplementation reverses the age-related decline in carnitine palmitoyltransferase 1 (CPT1) activity in interfibrillar mitochondria without changing the L-carnitine content in the rat heart PMID: 22322067
  9. sequence-dependence of oligomerization of transmembrane domain 2 of carnitine palmitoyltransferase 1A (CPT1A), to elucidate the role of this domain in the function of the full-length enzyme PMID: 21917985
  10. an environment-dependent structural switch underlies the regulation of carnitine palmitoyltransferase 1A PMID: 21990363
  11. The goal of the present study was to achieve more understanding on the modification/s of carnitinepalmitoyltransferase-I (CPT-I), the rate-limiting enzyme of the mitochondrial fatty acid beta-oxidation, during steatohepatitis. PMID: 21909411
  12. Acyl-CoA binding proteins interact with the acyl-CoA binding domain of mitochondrial carnitine palmitoyl transferase I PMID: 21541677
  13. strong protein-protein interaction between CPT1a, long chain acyl-CoA synthetase, and voltage-dependent anion channel PMID: 21622568
  14. pig and rat L-CPTI have different malonyl-CoA sensitivity, because the first 18 N-terminal amino acid residues interact differently with the C-terminal domain PMID: 11790778
  15. Adenovirus-mediated overexpression of liver carnitine palmitoyltransferase I in INS1E cells: effects on cell metabolism and insulin secretion. PMID: 11988095
  16. the last 31 C-terminal residues constitute a secondary structural determinant essential for the initial protein folding of L-CPTI PMID: 12351641
  17. L-CPT1 is not targeted to the endoplasmic reticulum membrane and that malonyl-CoA chloramphenicol acetyltransferase in microsomal fractions is L-CPT1 that is derived from mitochondria, possibly from membrane contact sites PMID: 12401113
  18. rat liver carnitine palmitoyltransferase has conserved residues essential for malonyl-CoA inhibition PMID: 12499375
  19. identification of conserved arginine and glutamate residues in C-terminal domain tha are improtant for catalytic activity and malonyl-CoA sensitivity PMID: 12540837
  20. Data show that either genetic or biochemical inhibition of hypothalamic carnitine palmitoyltransferase-1 activity was sufficient to substantially diminish food intake and endogenous glucose production. PMID: 12754501
  21. rat liver carnitine palmitoyltransferase I and its muscle isoform have similar malonyl-CoA sensitivity due to a single amino acid in the C-terminal domain of CPTI PMID: 12826662
  22. structural model for L-CPT I (liver CPT I), based on the similarity of this enzyme to the recently crystallized mouse carnitine acetyltransferase PMID: 14711372
  23. kinetic study of rat liver mitochondrial carnitine palmitoyltransferase-I PMID: 15247243
  24. mRNA steady-state levels of CPT I and mit HMG-CoA are not changed in rat DMBA-induced mammary cancer PMID: 15254779
  25. peroxisomal proliferator-activated receptor-gamma coactivator-1(PGC-1)alpha participates in stimulation of Carnitine palmitoyltransferase I (CPT-I) alpha gene expression by thyroid hormone suggesting that PGC-1 alpha is a thyroid hormone coactivator PMID: 15469941
  26. long-chain fatty acids regulate L-CPT I transcription through a PPARalpha-independent pathway, at least in hepatoma cells PMID: 16177188
  27. sequence spanning the loop-transmembrane (TM)2 boundary determines the disposition of this TM in the membrane so as to alter the conformation of the C-terminal segment and thus affect its interaction with malonyl-CoA PMID: 16908527
  28. LCPT I overexpression protects L6E9 myotubes from fatty acid-induced insulin resistance. PMID: 17062841
  29. These results demonstrate that PGC-1beta is a coactivator in the T3 induction of CPT-Ialpha and that PGC-1beta has similarities and differences with the PGC-1alpha isoform PMID: 17239528
  30. oligomeric structure of CPT1A would allow the newly formed acylcarnitines to gain direct access into the intermembrane space, hence facilitating substrate channeling. PMID: 17650509
  31. interventions that increase CPT1a activity could have potential benefits in the treatment of nonalcoholic fatty liver disease PMID: 18349115
  32. Accumulation of 3-hydroxylated intermediates of long-chain fatty acids may contribute to the pathogenesis of retinopathy in MTP deficiencies. PMID: 18385088
  33. Regulated over-expression of CPT1 is beneficial for glucolipotoxic beta-cells. PMID: 18706397
  34. A high-fat diet produces, but insulin resistance in the sham-treated muscle, insulin action is improved in the CPT1-overexpressing muscle in rats. PMID: 19073774
  35. molecular modeling of rCPT1A TM2 highlighted the favorable orientation of GXXXG and GXXXA motifs in the formation of the TM2 hexamer. PMID: 19136561
  36. Study highlights that CPT1A is a prime target to increase hepatic LCFA beta-oxidation and that acting directly on the degree of its malonyl-CoA sensitivity may be a relevant strategy to prevent and/or correct hepatic steatosis. PMID: 19302064

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Subcellular Location Mitochondrion outer membrane; Multi-pass membrane protein.
Protein Families Carnitine/choline acetyltransferase family
Tissue Specificity Liver and kidney.
Database Links

KEGG: rno:25757

STRING: 10116.ENSRNOP00000019652

UniGene: Rn.2856

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