CD47|Proteinase K|EGFR|BCMA|PD-L1|CD276|ACE2|TMPRSS2|Exosome Isolation Kits
Code | CSB-EAP33245 |
Size | US$1000 |
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Have Questions? | Leave a Message or Start an on-line Chat |
Uniprot No. | P0DTC2 |
Alternative Names | S, S1, S1-RBD, Spike glycoprotein |
Species Reactivity | Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) |
Immunogen | Llama with human IgG1 Fc |
Immunogen Species | Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) |
Concentration | It differs from different batches. Please contact us to confirm it. |
Form | Liquid |
Protein Names | Human Novel Coronavirus Spike glycoprotein (S) |
Host | Capture: Llama with human IgG1 Fc Detection: Mouse with human IgG1 Fc |
Buffer | Capture: 50% Glycerol, 0.01M PBS, PH 7.4 Detection: 50% Glycerol, 0.01M PBS, PH 7.4 |
Components | Capture: CSB-EAP33245C Detection: CSB-EAP33245B(HRP) Reagents are sufficient for at least 5 x 96 well plates using recommended protocol. |
Tested Applications | S-ELISA |
Troubleshooting and FAQs | Antibody FAQs |
Notes | We recommend using the capture antibody at a concentration of 1ug/ml and the detection antibody at a concentration of 0.42ug/ml.Optimal dilutions should be determined experimentally by the researcher. |
Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
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Have both antibodies been raised against the RBD domain of the S1 protein?
Which protein would you recommend as the standard for an ELISA assay?
Are there any recommendations for the concentrations of the different points of the standard curve?
Function | Spike glycoprotein comprises two functional subunits responsible for binding to the host cell receptor (S1 subunit) and fusion of the viral and cellular membranes (S2 subunit). For many coronavirus (CoVs), S is cleaved at the boundary between the S1 and S2 subunits, which remain non-covalently bound in the prefusion conformation. The distal S1 subunit comprises the receptor-binding domain(s) and contributes to stabilization of the prefusion state of the membrane-anchored S2 subunit that contains the fusion machinery. S is further cleaved by host proteases at the so-called S2' site located immediately upstream of the fusion peptide in all CoVs. This cleavage has been proposed to activate the protein for membrane fusion via extensive irreversible conformational changes. However, different CoVs use distinct domains within the S1 subunit to recognize a variety of attachment and entry receptors, depending on the viral species. Endemic human coronaviruses OC43 and HKU1 attach via their S domain A to 5-N-acetyl-9-O-acetyl-sialosides found on glycoproteins and glycolipids at the host cell surface to enable entry into susceptible cells. MERS-CoV S uses domain A to recognize non-acetylated sialoside attachment receptors, which likely promote subsequent binding of domain B to the entry receptor, dipeptidyl-peptidase 4. SARS-CoV and several SARS-related coronaviruses (SARSr-CoV) interact directly with angiotensin-converting enzyme 2 (ACE2) via SB to enter target cells. |
Gene References into Functions |
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Subcellular Location | Virion membrane, Single-pass type I membrane protein, Host endoplasmic reticulum-Golgi intermediate compartment membrane, Single-pass type I membrane protein, Host cell membrane, Single-pass type I membrane protein |
Protein Families | Betacoronaviruses spike protein family |