SARS-CoV-2 Spike RBD Antibody Pair 1

Code CSB-EAP33245
Size US$1000
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  • CSB-EAP33245 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). An antibody specific for SARS-CoV-2 Spike RBD has been pre-coated onto the microwells. The SARS-CoV-2 Spike RBD protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody HRP conjugated specific for SARS-CoV-2 Spike RBD is added to detect the captured SARS-CoV-2 Spike RBD protein. Followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
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Product Details

Uniprot No.
Alternative Names
S; 2; Spike glycoprotein; S glycoprotein; E2; Peplomer protein)
Species Reactivity
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Immunogen
Llama with human IgG1 Fc
Immunogen Species
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Concentration
It differs from different batches. Please contact us to confirm it.
Form
Liquid
Protein Names
Human Novel Coronavirus Spike glycoprotein (S)
Host
Capture: Llama with human IgG1 Fc
Detection: Mouse with human IgG1 Fc
Buffer
Capture: 50% Glycerol, 0.01M PBS, PH 7.4
Detection: 50% Glycerol, 0.01M PBS, PH 7.4
Components
Capture: CSB-EAP33245C
Detection: CSB-EAP33245B(HRP)
Reagents are sufficient for at least 5 x 96 well plates using recommended protocol.
Tested Applications
S-ELISA
Troubleshooting and FAQs
Notes
We recommend using the capture antibody at a concentration of 1ug/ml and the detection antibody at a concentration of 0.42ug/ml.Optimal dilutions should be determined experimentally by the researcher.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 2 reviews

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Applications : Optimization of analytical experiments

Sample type: Human

Review: Optimization of analytical conditions. (A) Optimization of spike protein antigen concentration from 1 µg mL−1 to 20 µg mL−1, (B) Effect of binding time between spike protein antigen and Ni(OH)2 NPs from 15 min to 65 min, (C) Effect of binding time between antibody and immobilized antigen from 5 min to 30 min, (each measurement was performed 3 times and the RSD averaged 1.5%).

By Anonymous

Applications : Enzyme-linked immunosorbent assay (ELISA)

Sample type: Vero cells

Review: Cells were infected with SARS-CoV-2 at 0.3 MOI and treated with APRG64, compounds 1, 7, or 12, at 5 or 25 µg/mL for 1 h. After washing three times with PBS, cells were incubated for an additional 72 h. Cell supernatants were analyzed for the SARS-CoV-2 spike proteins using enzyme-linked immunosorbent assay (ELISA).

By Anonymous

 Q&A
Q:

Have both antibodies been raised against the RBD domain of the S1 protein?

A:
Yes, both capture antibody and detection antibody are specific for RBD of S1 protein.
Q:

Which protein would you recommend as the standard for an ELISA assay?

A:
Q:

Are there any recommendations for the concentrations of the different points of the standard curve?

A:
Our experimental data of standards concentration as well as OD values is as below.
Q:

I am looking for a pair of antibodies for SARS-COV2 detection by a sandwich assay. Do you have any available?

A:

Cusabio supplies the following matched antibody pair for detecting SARS-CoV-2 Spike protein in Sandwich ELISA assay. Cusabio guarantees that the antibody works for the applications/species stated on our website and datasheet, a replacement or refund will be provided if the antibody does not work as described on our datasheet.

CSB-EAP33245 SARS-CoV-2 Spike RBD Antibody Pair 1 https://www.cusabio.com/Antibody-Pairs/S-Antibody-Pair-12928623.html
Concentration:
Capture Antibody: 1mg/ml
Detection Antibody: 0.42mg/ml

If you also need a standard protein, we would like to recommend the following product.
CSB-MP3324GMY1b1 https://www.cusabio.com/Recombinant-Protein/Recombinant-Human-Novel-Coronavirus-Spike-glycoprotein-S---partial--12928577.html

Target Background

Function
attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell. This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2. The stalk domain of S contains three hinges, giving the head unexpected orientational freedom. Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry. Can be alternatively processed by host furin. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.; mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.; Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.; May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.
Gene References into Functions
  1. Study presents crystal structure of C-terminal domain of SARS-CoV-2 (SARS-CoV-2-CTD) spike S protein in complex with human ACE2 (hACE2); hACE2-binding mode similar overall to that observed for SARS-CoV. However, details at the binding interface show that key residue substitutions in SARS-CoV-2-CTD slightly strengthen the interaction and lead to higher affinity for receptor binding than SARS-CoV receptor-binding domain. PMID: 32378705
  2. crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 bound to the cell receptor ACE2 PMID: 32365751
  3. crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 (engineered to facilitate crystallization) in complex with ACE2 PMID: 32320687
  4. Out of the two isolates from India compared to the isolates from Wuhan, China, one was found to harbor a mutation in its receptor-binding domain (RBD) at position 407 where, arginine was replaced by isoleucine. This mutation has been seen to change the secondary structure of the protein at that region and this can potentially alter receptor binding of the virus. PMID: 32275855
  5. Structural modeling of the SARS-CoV-2 spike glycoprotein show similar receptor utilization between SARS-CoV-2 and SARS-CoV, despite a relatively low amino acid similarity in the receptor binding module. Compared to SARS-CoV and all other coronaviruses in Betacoronavirus lineage B, an extended structural loop containing basic amino acids were identified at the interface of the receptor binding (S1) and fusion (S2) domains. PMID: 32245784
  6. crystal structure of CR3022, a neutralizing antibody from a SARS patient, in complex with the receptor-binding domain of the SARS-CoV-2 spike (S) protein to 3.1 A; study provides insight into how SARS-CoV-2 can be targeted by the humoral immune response and revealed a conserved, but cryptic epitope shared between SARS-CoV-2 and SARS-CoV PMID: 32225176
  7. SARS-CoV and SARS-CoV-2 spike proteins have comparable binding affinities achieved by balancing energetics and dynamics. The SARS-CoV-2-ACE2 complex contains a higher number of contacts, a larger interface area, and decreased interface residue fluctuations relative to the SARS-CoV-ACE2 complex. PMID: 32225175
  8. Interaction interface between cat/dog/pangolin/Chinese hamster ACE2 and SARS-CoV/SARS-CoV-2 S protein was simulated through homology modeling. Authors identified that N82 of ACE2 showed closer contact with receptor-binding domain of S protein than human ACE2. PMID: 32221306
  9. SARS-CoV-2 S glycoprotein harbors a furin cleavage site at the boundary between the S1/S2 subunits, which is processed during biogenesis and sets this virus apart from SARS-CoV and SARS-related CoVs; determined cryo-EM structures of the SARS-CoV-2 S ectodomain trimer. PMID: 32201080
  10. Study demonstrates that SARS-CoV-2 uses the SARS-CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming. PMID: 32155444
  11. The ACE2-B0AT1 complex exists as a dimer of heterodimers. Structural alignment of the RBD-ACE2-B0AT1 ternary complex with the S protein of SARS-CoV-2 suggests that two S protein trimers can simultaneously bind to an ACE2 homodimer. PMID: 32142651
  12. study demonstrated SARS-CoV-2 S protein entry on 293/hACE2 cells is mainly mediated through endocytosis, and PIKfyve, TPC2 and cathepsin L are critical for virus entry; found that SARS-CoV-2 S protein could trigger syncytia in 293/hACE2 cells independent of exogenous protease; there was limited cross-neutralization activity between convalescent sera from SARS and COVID-19 patients PMID: 32132184
  13. study determined a 3.5-angstrom-resolution cryo-electron microscopy structure of the 2019-nCoV S trimer in the prefusion conformation; provided biophysical and structural evidence that the 2019-nCoV S protein binds angiotensin-converting enzyme 2 (ACE2) with higher affinity than does severe acute respiratory syndrome (SARS)-CoV S PMID: 32075877

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Subcellular Location
Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein.
Protein Families
Betacoronaviruses spike protein family
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