Your Good Partner in Biology Research
Your Good Partner in Biology Research
| Code | CSB-EAP33245 |
| Size |
US$1000Purchase it in Cusabio online store (only available for customers from the US) |
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| Have Questions? | Leave a Message or Start an on-line Chat |
| Uniprot No. | P0DTC2 |
| Alternative Names | S; 2; Spike glycoprotein; S glycoprotein; E2; Peplomer protein) |
| Species Reactivity | Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) |
| Immunogen | Llama with human IgG1 Fc |
| Immunogen Species | Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) |
| Concentration | It differs from different batches. Please contact us to confirm it. |
| Form | Liquid |
| Protein Names | Human Novel Coronavirus Spike glycoprotein (S) |
| Host | Capture: Llama with human IgG1 Fc Detection: Mouse with human IgG1 Fc |
| Buffer | Capture: 50% Glycerol, 0.01M PBS, PH 7.4 Detection: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Components | Capture: CSB-EAP33245C Detection: CSB-EAP33245B(HRP) Reagents are sufficient for at least 5 x 96 well plates using recommended protocol. |
| Tested Applications | S-ELISA |
| Troubleshooting and FAQs | Antibody FAQs |
| Notes | We recommend using the capture antibody at a concentration of 1ug/ml and the detection antibody at a concentration of 0.42ug/ml.Optimal dilutions should be determined experimentally by the researcher. |
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
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Applications : Optimization of analytical experiments
Sample type: Human
Review: Optimization of analytical conditions. (A) Optimization of spike protein antigen concentration from 1 µg mL−1 to 20 µg mL−1, (B) Effect of binding time between spike protein antigen and Ni(OH)2 NPs from 15 min to 65 min, (C) Effect of binding time between antibody and immobilized antigen from 5 min to 30 min, (each measurement was performed 3 times and the RSD averaged 1.5%).
By Anonymous
Applications : Enzyme-linked immunosorbent assay (ELISA)
Sample type: Vero cells
Review: Cells were infected with SARS-CoV-2 at 0.3 MOI and treated with APRG64, compounds 1, 7, or 12, at 5 or 25 µg/mL for 1 h. After washing three times with PBS, cells were incubated for an additional 72 h. Cell supernatants were analyzed for the SARS-CoV-2 spike proteins using enzyme-linked immunosorbent assay (ELISA).
By Anonymous
Have both antibodies been raised against the RBD domain of the S1 protein?
Which protein would you recommend as the standard for an ELISA assay?
Are there any recommendations for the concentrations of the different points of the standard curve?
| Function (From Uniprot) |
attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell. This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2. The stalk domain of S contains three hinges, giving the head unexpected orientational freedom. Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry. Can be alternatively processed by host furin. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.; mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.; Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.; May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.
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| Gene References into Functions |
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| Subcellular Location | Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. |
| Protein Families | Betacoronaviruses spike protein family |
