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Applications : Optimization of analytical experiments
Sample type: Human
Review: Optimization of analytical conditions. (A) Optimization of spike protein antigen concentration from 1 µg mL−1 to 20 µg mL−1, (B) Effect of binding time between spike protein antigen and Ni(OH)2 NPs from 15 min to 65 min, (C) Effect of binding time between antibody and immobilized antigen from 5 min to 30 min, (each measurement was performed 3 times and the RSD averaged 1.5%).
Applications : Enzyme-linked immunosorbent assay (ELISA)
Sample type: Vero cells
Review: Cells were infected with SARS-CoV-2 at 0.3 MOI and treated with APRG64, compounds 1, 7, or 12, at 5 or 25 µg/mL for 1 h. After washing three times with PBS, cells were incubated for an additional 72 h. Cell supernatants were analyzed for the SARS-CoV-2 spike proteins using enzyme-linked immunosorbent assay (ELISA).
Have both antibodies been raised against the RBD domain of the S1 protein?
Which protein would you recommend as the standard for an ELISA assay?
Are there any recommendations for the concentrations of the different points of the standard curve?