The recombinant S antibody was prepared by obtaining the antibody genes, cloning the genes into a plasma vector to construct vector clone, transfecting the vector clone into a mammalian cell line for transient expression, and purifying the antibody by affinity-chromatography. This recombinant S antibody has been verified to detect the S protein from Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) in the ELISA, GICA.
SARS-CoV-2 S protein consists of two subunits S1 and S2. It is involved in receptor recognition, viral attachment, fusion, and entry into host cells. The S1 subunit contains a receptor-binding domain (RBD) responsible for the recognization and binding of the host receptor ACE2. The S2 subunit participates in the fusion of the viral envelope and host cell membrane. The S protein's central function in viral infection suggests that it could be a target for vaccines, antibody-blocking treatment, and small chemical inhibitors.