Purity
>96% as determined by SDS-PAGE and HPLC.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 10-100 ng/ml.
Alternative Names
C-C motif chemokine 13; CCL13; CCL13_HUMAN; Chemokine (C C motif) ligand 13; CK beta 10; CK-beta-10; CKb10; MCP-4; MGC17134; Monocyte chemoattractant protein 4; Monocyte chemotactic protein 4; NCC 1; NCC-1; NCC1; New CC chemokine 1; SCYA13; SCYL1; short chain; Small inducible cytokine A13 precursor; Small inducible cytokine subfamily A (Cys Cys) member 13; Small-inducible cytokine A13
Species
Homo sapiens (Human)
Expression Region
24-98aa
Complete Sequence
QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
5-10 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
This recombinant human CCL13 protein is expressed in E. coli and contains an amino acid sequence corresponding to the region 24-98aa of the human CCL13 protein. The product is tag-free and provided as a lyophilized powder, which can be reconstituted in sterile water or buffer before use. The purity of the protein is >96%, as determined by SDS-PAGE and HPLC, and the endotoxin level is less than 1.0 EU/µg, as measured by the LAL method. The recombinant CCL13 is fully biologically active when compared to the standard, and its activity has been determined by a chemotaxis bioassay using human monocytes at a concentration of 10-100 ng/ml.
C-C motif chemokine 13 (CCL13) is a small secreted protein belonging to the CC chemokine family. It is involved in the recruitment and activation of immune cells, particularly monocytes, and plays a crucial role in immune and inflammatory responses. Research in the area of immunology has been focused on understanding the function and regulation of CCL13 and its role in various immune-mediated diseases.
