Recombinant Human C-C motif chemokine 20 protein(CCL20) (Active)

In Stock
Code CSB-AP000931HU
Size US$3274Purchase it in Cusabio online store
(only available for customers from the US)
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity >97% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-50 ng/ml.
Target Names CCL20
Uniprot No. P78556
Research Area Immunology
Alternative Names Beta chemokine exodus 1; Beta-chemokine exodus-1; C C motif chemokine ligand 20; C-C motif chemokine 20; CC chemokine LARC; Ccl20; CCL20(2-70); CCL20_HUMAN; Chemokine (C C motif) ligand 20; Chemokine C-C motif chemokine 20; Chemokine CC motif ligand 20; CKb4; Exodus 1; Exodus; LARC; Liver and activation regulated chemokine; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP 3 alpha; MIP 3A; MIP-3-alpha; MIP-3a; MIP3 alpha; MIP3A; SCYA20; Small inducible cytokine A20 ; Small inducible cytokine subfamily A (Cys Cys) member 20; Small-inducible cytokine A20; ST38
Species Homo sapiens (Human)
Source E.Coli
Expression Region 27-96aa
Mol. Weight 8.0 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions
Gene References into Functions
  1. CCN1 stimulates CCL20 production in vitro and in vivo, and thus supports the notion that overexpressed CCN1 in hyperproliferating keratinocyte is functionally involved in the recruitment of inflammatory cells to skin lesions affected by psoriasis. PMID: 28602508
  2. Results demonstrated that F. nucleatum ssp. animalis and dysregulated CCL20 protein expression are important factors associated with human colorectal cancer progression. PMID: 28483840
  3. There was underexpression of the majority of genes after sunitinib treatment. The lower expression levels of IGFBP1, CCL20, CXCL6 and FGB were confirmed by qRT-PCR in all cases. The downregulation of gene expression leads us to search for methylation as a mechanism of action of the tyrosine kinase inhibitors PMID: 27834463
  4. CCL20 contributed to invasion and epithelial-mesenchymal transition of RANK over-expressed endometrial cancer cells PMID: 27015366
  5. High expression of CCL20 is associated with cutaneous T-cell lymphoma. PMID: 26789110
  6. These results showed that the higher levels of CXCL10, CCL20 and CCL22 were associated with ischemic heart disease. The serum levels of chemokines may influence by the certain traditional risk factors of IHD and some studied SNPs, but did not influence by treatment and gender of patients. PMID: 27152707
  7. The strongest OR was for CXCL8 (interleukin-8) in serum (96.8, 95% CI: 11.9-790.2). Of these 15 markers, 6 were also significantly elevated in serum from Chile (CCL20, C-reactive protein, CXCL8, CXCL10, resistin, serum amyloid A). PMID: 27173614
  8. CCR6(-) regulatory T cells blunt the restoration of gut Th17 cells along the CCR6-CCL20 axis in treated HIV-1-infected patients. PMID: 26883727
  9. CCL20 provoked a 2.5-fold increase of cell migration and invasion in human cancerous breast epithelial cells ; CCL20 also enhanced MMP- 2 and MMP-9 mRNAs/protein expression and activities. PMID: 28618084
  10. A gut-specific endogenously produced mediator with the capacity to modulate the IL-17/Th17 signaling response. PMID: 28526351
  11. results provide a potential explanation for involvement of the CCL20-CCR6 system in the trafficking of IL-17-producing cells to degenerated IVD tissues PMID: 23823618
  12. MFSD2B, CCL20 and STAT1, or STARD7 and ZNF512 genes may be risk or protect factors in prognosis of ADC; HTR2B, DPP4, and TGFBRAP1 genes may be risk factors in prognosis of SQC. PMID: 27301951
  13. HMC stimulated by IgA1 could produce CCL20 and consequently recruit inflammatory Th17 cells to the kidneys to induce further lesion in IgA nephropathy PMID: 28552941
  14. High CCL20 expression is associated with B-lymphoblastic lymphoma with inflammation. PMID: 27018255
  15. primary cultures of human bronchial epithelial cells and the BEAS-2B cell line were used to examine the effects of bacterial-viral coexposure, as well as each stimulus alone, on epithelial expression of CXCL8 and, in particular, CCL20. PMID: 28283475
  16. Study provides evidence that tumor cells signal to the surrounding healthy cells through CCL20 inducing the modulation of the expression of molecules involved in EMT and promoting angiogenesis directly and indirectly through the secretion of VEGF, a major contributor to angiogenesis. PMID: 26154142
  17. CCL20-recruited immune cells further increase TRAIL resistance of CCL20-producing pancreatic ductal adenocarcinoma cells. PMID: 28188806
  18. Cytomegalovirus replication in the allograft causes an intrapulmonary increase of CCL-18 and CCL-20 and a systemic rise of CCL-20 serum levels. PMID: 26910332
  19. This study evaluated the role of CCL20 and CCR6 in the regulation of laryngeal neoplasms; it showed that these proteins acted on proliferation and metastasis via the p38 pathway and multiple microRNAs. PMID: 27916417
  20. findings provide insight into the important role of macrophage-secreted CCL20 in pancreatic cancer and implicate CCR6/CCL20 as potential therapeutic targets PMID: 27797715
  21. Production of CCL20 from lung cancer cells induces the cell migration and proliferation through PI3K pathway. PMID: 26968871
  22. There were early increased plasma concentrations of CCL20 and CCR6 in patients with sepsis. CCL20 and CCR6 correlate with severity of illness in ICU patients. PMID: 26771764
  23. ADAM10 activity contributes to house dust mite-induced shedding of chemokines, including CCL20 PMID: 26296735
  24. Both MIP-3alpha and cystatin A overexpressions in NPC tumor tissues were strong independent factors of poor prognosis in NPC patients. PMID: 26634210
  25. High-risk HPV-type lesions might inhibit the chemokine CCL20 on Langerhans cells through E6 and E7 to escape the immune response. PMID: 26400278
  26. High CCL20 expression is associated with Inflammatory Bowel Disease. PMID: 26536229
  27. Tregs of unexplained recurrent miscarriage patients was significantly lower than that in controls. CCL20-CCR6 could drive immune activity of CD4(+)FOXP3(+) Tregs, followed by their migration to the feto-maternal microenvironment PMID: 26345847
  28. human lung epithelial cells secrete CCL20 and BD2 in response to B. abortus and/or to cytokines produced by infected monocytes. PMID: 26448160
  29. AEG-1 mediates CCL20/CCR6-induced EMT development via both Erk1/2 and Akt signaling pathway in cervical cancer, which indicates that CCL20/CCR6-AEG-1-EMT pathway could be suggested as a useful target to affect the progression of cervical cancer. PMID: 26156805
  30. CCL20 enhanced osteoblast-mediated osteoclastogenesis, partly via IL-6 production, suggesting that CCL20 may contribute to osteoporosis in rheumatoid arthritis by affecting bone cell communication. PMID: 26103626
  31. Th17 cells are recruited into tumor tissues preferentially through CCR6-CCL20 pathway PMID: 25768730
  32. High CCL20 expression is associated with colon cancer. PMID: 24866282
  33. stimulates leukocyte migration in ovary, may aid in final ovulatory events PMID: 26125463
  34. CCL20 cooperated with CCR6 could recruit T regulatory cells to tumor sites, and chemotherapy medicine docetaxel could decrease the expression of CCL20. PMID: 25661365
  35. CCL20 expression is associated with HCC recurrence and patient survival and promotes HCC cell proliferation and migration by inducing EMT-like changes via PI3K/AKT and Wnt/beta-catenin pathways. PMID: 25593462
  36. A TGF-beta-responsive enhancer element required for efficient IL-1beta-dependent expression of the proinflammatory chemokine CCL20 by human lung fibroblasts. PMID: 25918170
  37. Results indicate that CCL20 is crucial for tobacco smoke-caused lung cancer, and anti-CCL20 could be a rational approach to fight against this deadly disease. PMID: 25864589
  38. Overexpression of CCL20 in human proximal tubular cells is inhibited by blockade of KCa3.1 under diabetic conditions through inhibition of the NF-kappaB pathway. PMID: 24733189
  39. glucocorticoids enhance CCL20 production by bronchial epithelium, which may constitute a novel mechanism in Th17-mediated glucocorticoid-insensitive inflammation in asthma PMID: 24627531
  40. We conclude that CCL 20 mRNA expression in the conjunctival epithelium plays a crucial role in regulating homeostasis at the ocular surface and in exacerbation of vernal keratoconjunctivitis . PMID: 25172034
  41. CCL20 rs6749704 polymorphism was associated with secondary progressive multiple sclerosis. PMID: 24395091
  42. identify Syk as an upstream signaling molecule in IL-17A-induced Act1-TRAF6 interaction in keratinocytes, and inhibition of Syk can attenuate CCL20 production PMID: 25202827
  43. CCL20 could be an immune marker in ANCA-associated vasculitis. PMID: 25352172
  44. a differential regulation pattern for genes solely expressed in Th17 cells (IL17A and CCL20) compared to genes expressed in both Th17 and Th1 cells (IL23R and IL12RB2), where high levels of promoter methylation are correlated to near zero PMID: 22715389
  45. CCL20 levels were significantly elevated in the CSF of patients with pneumococcal meningitis. PMID: 24699535
  46. CCL20 and 14-3-3 zeta are molecules that play a putative role during tumorgenesis in pancreas, and may therefore be new parameters for histological diagnosis and discrimination between pancreatic neoplasms and chronic pancreatitis. PMID: 24629487
  47. CCR6/CCL20 biological axis increased the capacity of proliferation and adhesion, as well as the chemotactic migration and the level of cytokines related to degraded extracellular matrix. PMID: 24743888
  48. Data indicate that periodontal ligament stem cells (hPDLSCs) express a functional P2X7 ATP receptor, whose activation is linked to the production of pro-inflammatory molecules IL8 and CCL20. PMID: 24851271
  49. genetic and biological role of the C-C chemokine ligand CCL20 and the C-C chemokine receptor CCR6in rheumatoid arthritis, is discussed.[review] PMID: 24394994
  50. IL-6 produced from IL-1beta-stimulated human periodontal ligament cells could increase CCL20 production. PMID: 24081898
  51. CCL20 promotes the invasion and migration of thyroid cancer cells via NF-kappa B signaling-induced MMP-3 production. PMID: 24984269
  52. 1beta-stimulated HPDLCs. CONCLUSIONS: These data provide a novel mechanism through which genipin could be used to provide direct benefits in periodontal disease to inhibit IL-6 and CCL20 productions in periodontal lesions. PMID: 24557363
  53. CCL20 is an antimicrobial protein with bacteriocidal activity against E. coli and S. aureus. PMID: 12949249
  54. CCL20 and CXCL8 synergize to promote colorectal metastatic progression by coordinated induction of EMT via PI3K/AKT-ERK1/2 signaling axis. PMID: 24657657
  55. Dendritic and T cells in psoriasis express CCL20 and CCR6 as part of the pathology. PMID: 24401998
  56. High CCL20 expression in the microenvironment is associated with colorectal tumors. PMID: 23817679
  57. results suggest that plasmin generated in inflamed tissues might elicit production of chemokine CCL20 by human macrophages leading to the recruitment of CCR6 positive Th17 cells to the inflammatory sites PMID: 23681234
  58. results demonstrated that CCL20 might serve as a biomarker for GD and suggested the possible role of OPN in induction of CCL20 expression PMID: 23717583
  59. results strongly suggest an association between CCL20/CCR6 expression and the induction of colorectal adenoma, colorectal adenocarcinoma and the development of colorectal liver metastases PMID: 23790181
  60. In summary, tumor cells exchange signals with the surrounding healthy cells modifying the extracellular matrix through enzyme secretion; thus, CCL20 might be a factor involved in the ontogeny of breast carcinoma. PMID: 23460117
  61. Our data suggest for the first time that CCL20 and CCR6 might play an important role in the regulation of aggressiveness in human gliomas PMID: 22926920
  62. CXCR3 promotes recruitment of Th17 cells from the blood into the liver in both human and murine liver injury. Their subsequent positioning near bile ducts is dependent on CCR6 and cholangiocyte-secreted CCL20. PMID: 22796894
  63. In fibroblasts, the commensal bacterium Streptococcus gordonii stimulated the mRNA of CCL20 after 24 h, while not in gingival epithelial cells. PMID: 22860513
  64. Plasmin triggers release of the chemokine CCL20 from dendritic cells. PMID: 23118497
  65. Increased MIP-3a was significantly associated with synchronous liver metastases and poor prognosis in colorectal cancer patients. PMID: 22926691
  66. CCL20 may be used for the detection of HCC in HCV-infected patients with comparable specificity and higher sensitivity than AFP. PMID: 22388957
  67. identified CCAAT/enhancer binding protein beta as a critical regulator of CCL20 gene expression in normal human keratinocytes; HPV8 E7 oncoprotein suppressed C/EBPbeta-inducible and constitutive CCL20 gene expression as well as Langerhans cell migration PMID: 22911498
  68. These results suggest that Brucella induces only a weak proinflammatory response in gut epithelial cells, but produces a significant CCL20 secretion. PMID: 22553918
  69. CCL20 is a pivotal mediator of Langerhans cells recruitment into epidermis. PMID: 22857950
  70. Data show that transfection of ORMDL3 in bronchial epithelial cells induced expression of MMP-9, ADAM-8, CCL-20, IL-8, CXCL-10, CXCL-11, oligoadenylate synthetases (OAS) genes, and selectively activated activating transcription factor 6 (ATF6). PMID: 23011799
  71. MIP-3alpha-driven recruitment of Th17 cells or CD45R0+ memory T lymphocytes is not affected in patients carrying heterozygous NOD2 variants. PMID: 22286692
  72. CCL20 is one of its target genes, which is related to the HPV16 E6 and E7 oncogenes. PMID: 22001440
  73. CCL20/CCR6 may play an important role in the pathogenesis of RA by assembling the molecular and cellular components orchestrating synovitis. PMID: 21724701
  74. Data show IL-22 and CCL20, but not IL-17, expression is elevated in both sera and lesional skin in cutaneous T-cell lymphoma, and suggest that IL-22 may be important in cutaneous T-cell lymphoma, which may prove to be a future therapeutic target. PMID: 22048239
  75. miR-21 functionally interacts with the 3'UTR of CCL20 mRNA. PMID: 22099878
  76. Data show that IL-17A synergistically enhanced CCL20 production from IL-1beta-stimulated gingival fibroblasts (HGFs) in a concentration-dependent manner. PMID: 22019504
  77. The CCL20-CCR6 axis mediates the migration of circulating Tregs into tumor microenvironment, which in turn results in tumor progression and poor prognosis in HCC patients. PMID: 21935436
  78. Sorted keratinocytes had an early burst of upregulated gene expression, which included CCL20, IL-15, IL-23A, IFN-kappa, and several antimicrobial peptides. PMID: 21881590
  79. The role of the CCL20/CCR6/IL-17 axis in non-small-cell-lung-cancer tumor growth, was investigated. PMID: 21949768
  80. Data show that both CCL20 and CCR6 mRNA and protein expression levels were significantly increased in patients who received preoperative FOLFOX chemotherapy PMID: 21912453
  81. The ability of Fusobacterium nucleatum cell wall and beta-defensins to produce proinflammatory cytokines and CCL20 suggests the broad role of F. nucleatum and human antimicrobial peptides in primary immune responses elicited by oral epithelium. PMID: 21911466
  82. Gene expression of epithelial innate immune markers beta-defensin 2 and CC chemokine ligand 20 is increased following the inhibition of HDAC and DNMT PMID: 21248725
  83. Binding of CCL20, a G protein-coupled receptor ligand to G protein-coupled receptor CCR6 induces MUC5AC mucin production in human airway epithelial (NCI-H292) cells. PMID: 21300824
  84. Human corneal eptihelial cells secret CCL20 in response to S. aureus exposure. PMID: 21178448
  85. Polarized, in situ-differentiated, skin-derived T helper cell (Th)17 clones activated via the T cell receptor-CD3 complex produce CCL20 in addition to interleukin (IL)-17 and IL-22. PMID: 21178014
  86. M. tuberculosis increases dramatically the expression of CCL20 in human monocytes. CCL20 inhibits M. tuberculosis-mediated apoptosis, possibly as a consequence of the down-regulation of ROS production. PMID: 20819093
  87. Oxidative stress biases intestinal epithelial responses to flagellin, leading to increased production of IL-8 and decreased production of CCL20; suggesting that epithelial cells are capable of adjusting their antimicrobial responses. PMID: 20595617
  88. expression in gingival epithelial cells is induced by trypsin via activation of protease-activated receptor 2 PMID: 19567485
  89. Synergistically enhanced by interferon-gamma and tumor necrosis factor-alpha, the expression of CD54 in mesenchymal stem cells (MSCs) enables CCR6 chemokine ligand CCL20 to induce in vitro adhesion of T helper (Th)17 cells to MSCs. PMID: 20511548
  90. CCL20 and CCR6 play a role in the development and progression of pancreatic cancer and may constitute potential targets for novel treatment strategies. PMID: 20459729
  91. CCL20 was preferentially expressed in adrenal tissues from patients with primary non-small-cell lung cancer who developed metastases PMID: 20439195
  92. This study has shown that activation of OX40 induces CCL20 expression in the presence of antigen stimulation. PMID: 20400327
  93. higher levels of expression on peripheral blood mononuclear cells of chinese patients with inflammatory bowel disease PMID: 20064082
  94. Electric fields increased keratinocyte CCL20 expression. PMID: 19784662
  95. CCL20 and CXCR4 expressions are markedly down-regulated in epidermal condyloma acuminatum lesions. PMID: 17545018
  96. As CCL20 is activated by Th17 cytokines, the upregulation of CCL20 production by Trim32 provides a positive feedback loop of CCL20 and Th17 activation in the self-perpetuating cycle of psoriasis PMID: 20054338
  97. CCL20 (MIP3A) induces APOBEC3G expression in peripheral blood mononuclear cells and in CD4+ T lymphocytes via CCR6. The increased expression of APOBEC3G results in HIV inhibition. PMID: 20023216
  98. secretion in bronchial epithelial cells is induced during Chlamydophila pneumoniae infection PMID: 19479364
  99. adipocyte culture medium increased the production of TNF-alpha in MDA-MB-231 cells, which stimulated CCL20 expression in an autocrine fashion PMID: 19885605
  100. Data suggest that therapeutic efficacy of biologic DMARD may result from the inhibition of CCL20 production in rheumatoid synovium. PMID: 19797510

Show More

Hide All

Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Tissue Specificity Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level). Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intes
Database Links

HGNC: 10619

OMIM: 601960

KEGG: hsa:6364

STRING: 9606.ENSP00000351671

UniGene: Hs.75498

Most popular with customers


Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1