Dog Endothelin 1,ET-1 ELISA Kit

Instructions
Code CSB-E07002c
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name endothelin 1
Alternative Names EDN1Endothelin-1 ELISA kit; ET-1 ELISA kit; Preproendothelin-1 ELISA kit; PPET1) [Cleaved into: Big endothelin-1] ELISA kit
Abbreviation EDN1
Uniprot No. P13206
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 85-96  
1:2 Average % 94  
Range % 90-99  
1:4 Average % 89  
Range % 81-96  
1:8 Average % 100  
Range % 91-108  
Recovery
The recovery of dog ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 82-96  
EDTA plasma (n=4) 97 82-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.954 2.989 2.972 2.861  
100 2.388 2.398 2.393 2.282  
50 1.758 1.789 1.774 1.663  
25 1.035 1.069 1.052 0.941  
12.5 0.502 0.543 0.523 0.412  
6.25 0.271 0.268 0.270 0.159  
3.12 0.184 0.169 0.177 0.066  
0 0.112 0.110 0.111    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Endothelins are endothelium-derived vasoconstrictor peptides.
Gene References into Functions
  1. These findings suggest that elevated serum big ET-1 could be used as a diagnostic marker for canine hemangiosarcoma . PMID: 25920760
  2. This study showed that canine osteosarcoma cells express endothelin-1 and functional endothelin A receptors; increases in bone alkaline phosphatase activity are associated with osteoblastic OS lesions. PMID: 26426813
  3. There was a progressive increase in ET-1 and ETAR expression from benign tumour to grade 1 and grade 2 malignant mammary tumours with a decrease of expression in grade 3 carcinomas. Co-localization of ET-1 and ETAR was observed in benign mammary tumours. PMID: 25816929
  4. These findings point to the potential of serum big ET-1 as a clinical marker for cardiopulmonary and neoplastic diseases in dogs. PMID: 24447631
  5. Renal denervation substantially decreased BP and also significantly decreased the plasma levels of renin-angiotensin system and ET-1. PMID: 24496516
  6. These results showed that endothelin-1 and endothelin receptor A are overexpressed in canine ovarian tumours, suggesting a potential role of these two molecules in canine ovarian carcinogenesis. PMID: 20210880
  7. ET-1 production in macrophage cell line DH82 was increased in response to stimulation with intact and viable gram-positive and gram-negative bacteria. PMID: 20207425
  8. The results showed that there was an interaction between ET-1 and NO in a VILI (ventilator-induced lung injury )model and changing the balance of ET-1 and NO levels might contribute to the pathophysiologic process of VILI. PMID: 20497708
  9. Report endothelin levels in experimental diabetes combined with cardiac hypertrophy. PMID: 15838277
  10. Report changes in endothelin-1 gene expression during myocardial ischemia and reperfusion. PMID: 15838278
  11. Intrapericardial adenine nucleosides induce elevation of endothelin-1 concentration in the pericardial space of the dog heart. PMID: 15838286
  12. The results confirm that intrapericardial catecholamines exert significant hemodynamic effects and modulate ET-1 and adenosine release from the heart. PMID: 15838288
  13. Report changes in endothelin-1/big endothelin-1 levels and action potential duration during myocardial ischemia-reperfusion in dogs with and without ventricular fibrillation. PMID: 15838324
  14. although ET(A) receptor-signaling is sensitized by induction of the metabolic syndrome, endothelin-mediated coronary vasoconstriction does not significantly contribute to coronary dysfunction at this early stage of prediabetes PMID: 16627363
  15. These results may potentially explain resting perfusion abnormalities or heterogeneity by clinical PET that may persist or only partially improve after adenosine stress perfusion imaging in the absence of myocardial scar and flow-limiting stenosis. PMID: 16951040
  16. Alterations in blood levels of endothelin-1, atrial natriuretic peptide, aldosterone, and norepinephrine in dogs with and without dilated cardiomyopathy are reported. PMID: 17338155
  17. Early pulmonary hypertension in heart failure is characterized by altered vasoreactivity and increased ET-1 and VEGF2 signalling. PMID: 17993509
  18. peripheral NO and ET-1 release modulates the cardiac contractility through myocardial ET-A receptor activation and mast cell degranulation. PMID: 18788466
  19. The use of Plasma atrial natriuretic peptide (proANP 31-67), B-type natriuretic peptide (Nt-proBNP) and endothelin-1 (ET-1) concentrations as disease markers in dogs with valvular heart disease is reported. PMID: 19585255

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Subcellular Location Secreted
Protein Families Endothelin/sarafotoxin family
Database Links

KEGG: cfa:403424

STRING: 9615.ENSCAFP00000014389

UniGene: Cfa.125

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