Human Interleukin 9,IL-9 ELISA KIT

Code CSB-E04642h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human IL9 ELISA Kit was designed for the quantitative measurement of Human IL9 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Name interleukin 9
Alternative Names Cytokine P40 ELISA Kit; Homolog of mouse T cell and mast cell growth factor 40 ELISA Kit; HP40 ELISA Kit; IL 9 ELISA Kit; IL-9 ELISA Kit; Il9 ELISA Kit; IL9_HUMAN ELISA Kit; Interleukin 9 ELISA Kit; Interleukin-9 ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; Megakaryoblast growth factor ELISA Kit; P40 ELISA Kit; p40 cytokine ELISA Kit; p40 T cell and mast cell growth factor ELISA Kit; T cell growth factor 3 ELISA Kit; T cell growth factor p40 ELISA Kit; T-cell growth factor P40 ELISA Kit; TCGF 3 ELISA Kit
Abbreviation IL9
Uniprot No. P15248
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %100
Range %97-103
1:2Average %99
Range %94-103
1:4Average %101
Range %98-106
1:8Average %97
Range %91-103
Recovery
The recovery of human IL-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8380-86
EDTA plasma (n=4)9489-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.403 2.498 2.451 2.306
5001.520 1.568 1.544 1.399
2500.845 0.898 0.872 0.727
1250.523 0.568 0.546 0.401
62.50.365 0.367 0.366 0.221
31.20.282 0.271 0.277 0.132
15.60.221 0.219 0.220 0.075
00.143 0.146 0.145
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Supports IL-2 independent and IL-4 independent growth of helper T-cells.
Gene References into Functions
  1. this study shows that intensified IL-9 production is associated with the immunopathogenesis of active ulcerative colitis PMID: 29927662
  2. IL9 CNS-25/IL9 CNS-18 is a critical and conserved regulatory element for IL-9 production in mice and humans. PMID: 30442929
  3. CXCL10, not CXCL9 or CXCL11, induced IL-9 expression in the liver tissue. PMID: 29860220
  4. Our study reveals that degeneration and destruction of cartilage may be related to the production of IL-9 in osteonecrosis of the femoral head patients. In a human primary chondrocytes culture model, IL-9 increased the degeneration of cartilage; blocking JAK-STAT signaling alleviated this effect. PMID: 29775946
  5. Having one or more copies of the 1635A allele was associated with increased cytomegalovirus acquisition in HIV-infected infants (42 vs. 11%, P = 0.03) and increased risk of Epstein-Barr virus acquisition in HIV-exposed uninfected infants (hazard ratio = 4.2, P = 0.02) compared with 1635GG. PMID: 29112074
  6. T Helper 9 cells may be the major source of interleukin-9 (IL-9) in children with allergic asthma. In these patients, IL-9 impairs interferon gamma production and synergistically promotes interleukin-4-induced IgE secretion. PMID: 28724400
  7. Increased expression of nuclear factor of activated T cells 1 drives IL-9-mediated allergic asthma. PMID: 26993036
  8. these results demonstrated that IL-9-expressing Th9 cells were upregulated in breast cancer patients and potentially possessed antitumor roles by enhancing CD8+ T cell-mediated cytotoxicity PMID: 28918288
  9. Serum IL-9 and IL-22 are associated with eosinophilia in cow's milk allergy, and decrease in these two cytokines with occurs with cow's milk elimination. PMID: 28934137
  10. The systemic IL9 level is higher in ulcerative colitis and corresponds with endoscopic inflammation, suggesting its possible application as a negative marker of mucosal healing. PMID: 28652656
  11. These findings suggest that IL-9 is involved in the pathogenesis of VKH disease, and that IL-9 might also enhance the inflammatory response by increasing the secretion of IL-17, an established proinflammatory cytokine in VKH disease PMID: 28761327
  12. Data suggest that the conditions defined for strong induction of interleukin-9 (IL-9) might be relevant for the development of Vdelta2 T-cell-based immunotherapy. IL-9 might be relevant for the development of Vdelta2 T-cell-based immunotherapy. PMID: 27791087
  13. An aberrant expression profile of Th9/IL-9 was associated with pathogenesis of immune thrombocytopenia, possibly through cooperative interaction with Th17/IL-17. PMID: 27662073
  14. In vitro data indicate that IL9 is regulated by STAT3/5 and in vivo results highlight the pro-neoplastic effect of IL9 on lymphoma T cells. The results suggest that IL9 and its regulators are promising new targets for therapy development in mycosis fungoides. PMID: 26851186
  15. recent findings suggest that blockade of IL-9 signaling is effective in treating experimental models of autoimmune and chronic inflammatory diseases such as inflammatory bowel diseases, allergic disorders such as food allergy and asthma. PMID: 26976761
  16. Findings showed that serum levels of IL-9 were elevated in diffuse large-B-cell lymphoma (DLBCL) patients and positive expression of IL-9 was correlated with adverse prognosis indicators. It directly effected proliferation and apoptosis of DLBCL cells by enhancing the expression of p21CIP1 genes and promoted tumor cells to display resistance to chemotherapeutic drugs. PMID: 27364124
  17. no differences were found in serum levels of IL-9 between different clinical forms of periportal fibrosis in human Schistosoma mansoni infection in Brazil PMID: 27506138
  18. PU.1 and IL-9 may play a role in AD pathogenesis and relate to disease severity and clinical eruption types. PMID: 28229452
  19. Inflammatory cell expression of IL-9 and IL-17C were increased in chronic rhinosinusitis, particularly with allergy and asthma. These interleukins may contribute to the pathogenesis of chronic rhinosinusitis with nasal polyps as well as atopy and may serve as therapeutic targets for disease management PMID: 26989880
  20. serum levels determined of both T cell polarizing (IL- 33 and IL-12) and T cell effector (IFN-gamma, IL-4, IL-10, IL-17 and IL-9) cytokines in T1DM (type-1 Diabetes Mellitus) subjects with and without microvascular complications (MVC). All the tested cytokines were significantly elevated in T1DM subjects except for IFN-gamma with no significant difference between those with and without MVC. PMID: 27442004
  21. results suggest that Th9 cells and IL-9 could play an important role in the pathogenesis of systemic sclerosis by modulating adaptive and innate immune responses and the production of autoantibodies and indicate the IL-9 pathway as a possible therapeutic target PMID: 28681919
  22. TL1A differentially induces expression of TH17 effector cytokines IL-17, -9, and -22 and provides a potential target for therapeutic intervention in TH17-driven chronic inflammatory diseases. PMID: 27733581
  23. These data indicate that IL-9 is an essential regulator of megakaryopoiesis and a promising therapeutic agent for treatment of thrombocytopenia such as CIT. PMID: 28450306
  24. Expression of IL-9 remarkably increases in peripheral blood and liver tissues in patients with primary biliary cirrhosis PMID: 27916102
  25. IL-9 promotes proliferation and metastasis in pancreatic cancer cells; this effect may partly involve regulation of the miR-200a/b-catenin axis. PMID: 28349057
  26. in PsA patients gammadelta T cells activation is driven prevalently by IL-9/IL-9R interaction, and not only by IL-23/IL-23R. Together these findings indicate gammadelta T cells and IL-9 as new players in the pathogenesis of PsA. PMID: 27543964
  27. Th9 cells produce IL9 that may participate in pathogenesis of Takayasu's arteritis PMID: 27629397
  28. IL-9 serum leves are elevated in patients with systemic lupus erythematosus, rheumatoid arthritis and systemic sclerosis but their clinical significance is unknown. [review] PMID: 26921642
  29. Th9 cell numbers and IL-9 levels are correlated with OA patient symptoms and joint functionality PMID: 26926842
  30. IL-9 is functionally active, and is a pro-growth/survival factor for the localized pathologic T cells in the synovium of inflammatory arthritis. PMID: 26751012
  31. Asthmatic children showed an increase in plasma Il9 levels post Mycoplasma pneumoniae infection. PMID: 26191227
  32. Interleukin-9 Is Associated with Elevated Anti-Double-Stranded DNA Antibodies in Lupus-Prone Mice PMID: 25902303
  33. Data demonstrated increased serum levels of IL-9 in systemic lupus erythematosus and rheumatoid arthritis patients but no association with clinical and laboratory parameters was found. PMID: 26078482
  34. identified as a gene target that might interact with environmental exposure to dust mite allergen to increase asthma severity in children PMID: 25913104
  35. association between IL-9 and diabetic kidney disease was complex as it was reduced in diabetes mellitus, but it was of normal levels in those with diabetic kidney disease PMID: 25542095
  36. secretion increased in respiratory epithelial adenomatoid hamartoma tissue PMID: 26131817
  37. The results provide evidence that IL-9 is predominantly involved in the pathogenesis of ulcerative colitis suggesting that targeting IL-9 might become a therapeutic option for patients with UC. PMID: 24957265
  38. Serum IL-9 is closely related to several clinical features, such as age, B symptoms and local lymph node involvement. It can also be an independent prognostic factor for extranodal NK/T-cell lymphoma, which suggests a role for IL-9 in disease pathogenesis PMID: 24722378
  39. Upregulation of IL-9 induced by pSTAT6 may be involved in the pathogenesis of chronic lymphocytic leukemia. PMID: 24966942
  40. IL-9 is a key component of memory TH cell peanut-specific responses from children with peanut allergy (PA) and may be a useful biomarker to distinguish between children with PA and those with peanut sensitization. PMID: 25112699
  41. Overexpression of IL-9 may contribute to the pathogenesis of chronic lymphocytic leukemia and is associated with some adverse prognostic parameters. PMID: 24551294
  42. This study demonstrates that IL-9, through its direct effects on Th1 and ability to promote IL-4 secretion, has a regulatory role for Th1 lymphocytes in allergic contact dermatitis. PMID: 24487305
  43. A role for the IL-9/IL-9R axis in the atherosclerotic process, is reported. PMID: 24023645
  44. IL-9 production by memory T-helper (TH)9 cells is higher in the peripheral blood of patients with asthma than in healthy donors. PMID: 24468256
  45. Recruitment of interleukin 9-producing CD4+ T cells into malignant pleural effusion could be induced by pleural CCL20 and that the majority of Th9 cells in MPE displayed the phenotype of effector memory cells. PMID: 23700286
  46. The activator protein 1 (AP1) family transcription factor BATF (B cell, activating transcription factor-like) was among the genes enriched in Th9 cells and was required for the expression of IL-9 and other Th9-associated genes. PMID: 24216482
  47. the expansion of the Th9 cell subset, up-regulation of the PU.1 transcription factor and increased secretion of the IL-9 cytokine may contribute to the pathogenesis of AD PMID: 24032555
  48. Higher numbers of TH9 cells occur in normal human skin & blood vs metastatic lesions of progressive stage IV melanoma. These results suggest a role for IL-9 in tumor immunity. PMID: 22772464
  49. Children with atopic dermatitis may have higher serum IL-9 levels than healthy children, and IL-9 levels are significantly related to symptom severity. PMID: 22612419
  50. Substantial renal IL-9 release was observed from deceased donor kidneys PMID: 22971662

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Subcellular Location Secreted.
Protein Families IL-7/IL-9 family
Database Links

HGNC: 6029

OMIM: 146931

KEGG: hsa:3578

STRING: 9606.ENSP00000274520

UniGene: Hs.960

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