Human Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Code CSB-E06883h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01236
Lead Time 3-5 working days
Abbreviation PRL
Protein Biological Process 1 Sex hormone
Target Name prolactin
Alias N/A
Species Homo sapiens (Human)
Protein Biological Process 3 Lactation
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 40 μIU/mL-2000 μIU/mL
Sensitivity 50 μIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 9089
RGD 621047
MGI 1858197
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 86-97  
1:2 Average % 92  
Range % 87-100  
1:4 Average % 95  
Range % 92-99  
1:8 Average % 95  
Range % 90-98  
Recovery
The recovery of human PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-102  
EDTA plasma (n=4) 97 92-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/ml OD1 OD2 Average Corrected  
2000 2.186 2.105 2.146 2.115  
800 1.201 1.197 1.199 1.168  
300 0.622 0.619 0.621 0.590  
100 0.296 0.302 0.299 0.268  
40 0.145 0.148 0.147 0.116  
0 0.032 0.030 0.031    
References
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Function Prolactin acts primarily on the mammary gland by promoting lactation.
Subcellular Location Secreted
Protein Families Somatotropin/prolactin family
Database Links

HGNC: 9445

OMIM: 176760

KEGG: hsa:5617

STRING: 9606.ENSP00000302150

UniGene: Hs.1905

Pathway Jak-STAT signaling pathway
PI3K-Akt signaling pathway

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