Human Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Code CSB-E06883h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


The product CSB-E06883h is a sandwich ELISA kit developed to measure levels of human prolactin (PRL) in multiple samples, including serum, plasma, cell culture supernates, tissue homogenates, or cell lysates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of PRL bound in the initial step.

PRL, also called luteotropic hormone (LTH), is a polypeptide hormone responsible for lactation, certain breast tissue development, and hundreds of other bodily processes required to maintain homeostasis. The level of PRL is prominent for normal lactational capabilities. Imbalances in PRL levels can compromise this ability. Too low PRL levels make a mother will not be able to produce milk, while PRL levels are inappropriately elevated, this can lead to galactorrhea in non-breastfeeding females or males. Disruption in the PRL balance can have significant effects on the menstrual cycle.

Target Name prolactin
Alternative Names PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
Abbreviation PRL
Uniprot No. P01236
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 40 μIU/mL-2000 μIU/mL
Sensitivity 50 μIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 86-97  
1:2 Average % 92  
Range % 87-100  
1:4 Average % 95  
Range % 92-99  
1:8 Average % 95  
Range % 90-98  
The recovery of human PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-102  
EDTA plasma (n=4) 97 92-99  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/ml OD1 OD2 Average Corrected  
2000 2.186 2.105 2.146 2.115  
800 1.201 1.197 1.199 1.168  
300 0.622 0.619 0.621 0.590  
100 0.296 0.302 0.299 0.268  
40 0.145 0.148 0.147 0.116  
0 0.032 0.030 0.031    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Prolactin acts primarily on the mammary gland by promoting lactation.
Gene References into Functions
  1. Circulating prolactin levels may not be higher in patients with Behcet's disease than in controls (meta-analysis). PMID: 29412808
  2. Cin85-deficient mother mice had reduced pituitary hormone prolactin secretion as a result of excessive dopamine signaling in the brain. Their offspring matured normally and produced their own pups; however, nurturing behaviors such as pup retrieval and nursing were strongly inhibited. PMID: 29158391
  3. PRL levels were lower in psychogenic non-epileptic seizure patients with a history of sexual abuse compared to healthy controls and healthy controls with a history of sexual abuse. PMID: 28927333
  4. In newborns, serum PRL and hGH levels show a positive correlation that can be explained by common regulatory factors or a drift phenomenon. A higher gestational week is associated with a higher PRL/hGH ratio. PMID: 28700563
  5. High normal circulating prolactin levels predict changes in haemodynamic indices and worsening endothelial function in healthy postmenopausal women. Particularly in young postmenopausal women, prolactin predicts accelerated arterial stiffening. PMID: 28332508
  6. In conclusion, the present study provides evidence supporting a significantly positive association between plasma prolactin levels and the risk of breast cancer. PMID: 27184120
  7. Our studies have demonstrated the essential role of endogenous PRL and CDK7 in the upregulation of PRLR by E2 and provide insights for therapeutic approaches that will mitigate the transcription/expression of PRLR and its participation in breast cancer progression fueled by E2 and PRL via their cognate receptors. PMID: 28423697
  8. GABA alpha2 and/or alpha3 receptor subtypes are involved in GABAergic modulation of prolactin secretion. PMID: 27128218
  9. This study revealed a significant association between the prolactin polymorphic variant rs1341239 and the development of hyperprolactinemia in patients with schizophrenia. PMID: 27776952
  10. High serum prolactin and vasoinhibin levels predict and may impact retinopathy of prematurity progression PMID: 27842054
  11. The production of PRL by Mvarphis was increased by unknown components of rheumatoid arthritis and rheumatoid arthritis synovial fluid, where it could contribute to disease progression. PMID: 28642278
  12. findings show that cysteine in the PRL catalytic site is endogenously phosphorylated as part of the catalytic cycle and that phosphocysteine levels change in response to Mg(2+) levels. PMID: 27856537
  13. Depression and anxiety in subfertile males are associated with higher secretion of PRL. PMID: 28456148
  14. Prolactin rs1341239 T allele decreases the risk of brick tea fluorosis in the Inner Mongolia in China population. PMID: 28152004
  15. In breast cancer, PRL hormone and its signaling pathway may play an important role in maintaining tumor differentiation state and in turn better patient outcome. PMID: 26980025
  16. Data indicate 12 single nucleotide polymorphism (SNPs) associated with increased prolactin levels in both plasma and cerebrospinal fluid (CSF). PMID: 27357110
  17. these data strongly support a critical interplay between prolactin and estrogen via PAK1 and suggest that ligand-independent activation of ERalpha through prolactin/PAK1 may impart resistance to anti-estrogen therapies. PMID: 26944939
  18. These data provide insight into the mechanisms guiding PRL-mediated breast cancer cell motility and invasion and highlight a significant role for phosphoTyr-PAK1 in breast cancer metastasis. PMID: 27542844
  19. changes in mRNA expression of selected toll-like receptors (TLR2, TLR4, TLR7), stress cytokine prolactin (PRL), and proand anti-inflammatory cytokines (TNF-alpha, IL-6, IL-12, IL-10) in peripheral blood monocytes of celiac disease patients, were investiagted. PMID: 28028951
  20. The review focuses on the role of prolactin in breast development, bone homeostasis and in breast cancer to bone metastases. (Review) PMID: 27782069
  21. Study demonstrate that PRL is necessary for the survival of (retinal pigment epithelium) RPE under normal and advancing age conditions and, identified SIRT2 and TRPM2 as molecular targets for the antioxidant and antiapoptotic actions of PRL in the RPE. PMID: 27322457
  22. This review addresses risk factors for rheumatoid arthritis (RA) linked to prolactin (PRL), the effects of PRL and vasoinhibins on joint tissues, blood vessels, and immune cells, and the clinical and experimental data associating PRL with RA. [review] PMID: 27026299
  23. Data suggest that the serum level of PRL was closely related to the antibody production and disease activity of systemic lupus eruthematosus patients. PRL concentration was dramatically reduced upon the remission of disease activity. PMID: 26678441
  24. prolactin levels and pituitary adenoma diameters may be associated with increased risk of cancer in patients with prolactinoma PMID: 26720589
  25. reduction in prolactin level is frequent after SRS and FSRT for prolactinomas; however, true biochemical remission is uncommon. PMID: 25410143
  26. the -1189 estrogen response element is the dominant response element involved in the human PRL transcriptional response. PMID: 26691151
  27. Data suggest that plasma kisspeptin and serum prolactin levels may be involved in the physiopathology of breast enlargement in newborns. PMID: 26831552
  28. Serum PRL was significantly higher in chronic hepatitis C patients compared to controls. PMID: 25912488
  29. PAK1 nuclear translocation is ligand-dependent: only PRL but not E2 stimulated PAK1 nuclear translocation PMID: 27003261
  30. Mean serum prolactin level was higher in the patients with recurrent miscarriage than in healthy women. PMID: 26370377
  31. findings revealed that the -1149TT genotype was correlated with higher levels of prolactin in serum and prolactin gene expression in peripheral blood mononuclear cells in women with systemic lupus erythematosus; study suggests that the -1149TT genotype may be a risk factor for lupus PMID: 26583155
  32. The results of our study suggest the association between vitamin D status and elevated macroprolactin levels in premenopausal women. PMID: 26179930
  33. Despite the fact that serum prolactin levels were found to be high during pregnancy, the contribution of macroprolactin was found to be insignificant. No relationship between thyroid autoimmunity and PRL, macroPRL or monoPRL % was found. PMID: 26091810
  34. PRL is ectopically activated in a subset of very aggressive lung tumors, associated with a rapid fatal outcome, in a cohort of 293 lung tumor patients and in an external independent series of patients; analysis of the PRL transcripts in lung tumors and cell lines revealed systematic truncations of their 5' regions, including the signal peptide-encoding portions. PMID: 24512221
  35. PRL-PRLR can escalate the impact of breast cancer on bone metastasis and the presence of PRLR in the tumor microenvironment of breast cancer bone metastasis has the potential to modulate the microenvironment to induce lytic osteoclast formation. PMID: 26586670
  36. Women with higher prolactin levels did not report significantly increased hostility. PMID: 25226401
  37. results suggest that up-regulation of IL-10 by prolactin might be modulating the pro-inflammatory response against mycobacterial antigens through the MAPK pathway PMID: 25218920
  38. there is a link between prolactin and mean platelet volume levels; in women with PCOS, elevated PRL levels may increase the risk of developing atherothrombotic events via the activation of platelets PMID: 25359296
  39. Our data extends prior research linking prolactin and invasive breast cancer to the outcome of in situ breast tumours and shows that higher circulating prolactin is associated with increased risk of in situ breast cancer. PMID: 25887963
  40. Cord blood prolactin level showed weak correlation with duration of full obstetric delivery, and duration of the second stage. PMID: 26160091
  41. the prolactin -1149 G/T polymorphism is associated with susceptibility to RA, but not SLE, in Caucasians [meta-analysis] PMID: 25724680
  42. Patients with diabetes mellitus and impaired renal function demonstrate a high urinary PRL excretion. PMID: 26299069
  43. serum levels were found to be higher in the infertile women than in controls PMID: 25345488
  44. The prevalence and predictors of hyperprolactinemia in 178 patients with liver cirrhosis of different etio-logies, were investigated. PMID: 24816831
  45. Studied the serum prolactin, cortisol, and ACTH stress response of intensive care unit (ICU) patients with severe sepsis/septic shock (SS) or systemic inflammatory response syndrome (SIRS) compared to healthy subjects. PMID: 25243181
  46. Report positive association between prolactin levels and the damage index (overall and its renal and cardiac/peripheral vascular domains), independently of other well-known risk factors in systemic lupus erythematosus. PMID: 24718588
  47. Hypertensive postmenopausal women with low risk for cardiovascular diseases have increased levels of prolactin, suggesting a possible role in the pathogenesis of hypertension PMID: 24861467
  48. a gene signature based on PRL-upregulated genes was associated with prolonged relapse-free and metastasis-free survival in breast cancer patients. PMID: 23758962
  49. REVIEW: Hypothalamic pituitary adrenal axis and prolactin abnormalities in suicidal behavior PMID: 24040800
  50. PRL constrains tumor-promoting liver inflammation. PMID: 25049387

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Subcellular Location Secreted.
Protein Families Somatotropin/prolactin family
Database Links

HGNC: 9445

OMIM: 176760

KEGG: hsa:5617

STRING: 9606.ENSP00000302150

UniGene: Hs.1905

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