Human Protein Wnt-4(WNT4) ELISA kit

Code CSB-EL026137HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P56705
Lead Time 5-7 working days
Abbreviation WNT4
Protein Biological Process 1 Signaling Pathway
Target Name wingless-type MMTV integration site family, member 4
Alias RP1-224A6.7, SERKAL, WNT-4, WNT-4 protein
Species Homo sapiens (Human)
Protein Biological Process 3 Wnt signaling pathway
Sample Types serum, plasma, tissue homogenates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Stem Cells
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome.
HGNC 12783
RGD 621348
MGI 98957
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human WNT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %84
Range %80-92
1:2Average %98
Range %91-105
1:4Average %97
Range %92-110
1:8Average %93
Range %86-96
Recovery
The recovery of human WNT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9289-95
EDTA plasma (n=4)9790-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.312.32.305 2.133
7501.8431.8451.844 1.672
3751.3311.3371.334 1.162
187.50.8560.8550.856 0.684
940.630.6210.626 0.454
470.4190.4140.417 0.245
23.50.2950.2970.296 0.124
00.1710.1730.172  
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Function Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast tissue.
Involvement in disease 46,XX sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL); Mullerian aplasia and hyperandrogenism (MULLAPL)
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Wnt family
Database Links

HGNC: 12783

OMIM: 158330

KEGG: hsa:54361

STRING: 9606.ENSP00000290167

UniGene: Hs.25766

Pathway Hippo signaling pathway
Melanogenesis
mTOR signaling pathway
Wnt signaling pathway
Signaling pathways regulating pluripotency of stem cells

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