Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

WNT4

Uniprot No.

P56705

Research Area

Signal Transduction

Alternative Names

MGC123964; Protein Wnt-4; RP23-246F18.1; SERKAL; Wingless type MMTV integration site family member 4 precursor; WNT 4; WNT 4 protein precursor; WNT4; WNT4_HUMAN

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

23-351aa

Target Protein Sequence

SNWLYLAKLSSVGSISEEETCEKLKGLIQRQVQMCKRNLEVMDSVRRGAQLAIEECQYQFRNRRWNCSTLDSLPVFGKVVTQGTREAAFVYAISSAGVAFAVTRACSSGELEKCGCDRTVHGVSPQGFQWSGCSDNIAYGVAFSQSFVDVRERSKGASSSRALMNLHNNEAGRKAILTHMRVECKCHGVSGSCEVKTCWRAVPPFRQVGHALKEKFDGATEVEPRRVGSSRALVPRNAQFKPHTDEDLVYLEPSPDFCEQDMRSGVLGTRGRTCNKTSKAIDGCELLCCGRGFHTAQVELAERCSCKFHWCCFVKCRQCQRLVELHTCR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

52.7kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombinant Human Protein Wnt-4 (WNT4) is a partial-length protein expressed with an N-terminal 6xHis-SUMO-tagged in the E.coli. Its expression region corresponds to 23-351aa of human WNT4 protein. Its purity was determined by SDS-PAGE and reached up to 90% and presented a molecular mass band around 45 kDa on the gel. This recombinant WNT4 protein may be used to synthesize antibodies against WNT4 or on the studies of WNT4-associated signal transduction. This recombinant WNT4 proteins in-stock are available.
WNT4, ligand for members of the frizzled family of seven transmembrane receptors, plays an important role in the embryonic development of the urogenital tract and the lung. This protein shows 98% amino acid identity to the Wnt4 protein of mouse and rat. WNT4 mutations can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome.

Customer Reviews and Q&A

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■ Q&A

Is there any other reagent that can be used for long-term storage because grycerol is toxic to the cells? What % of BSA is recommended when we use human ES cells?

If you prefer to avoid adding glycerol after reconstituting for long-term storage, we have two suggestions:
1. Store the liquid format protein at -80°C.
2. Select an alternative reagent based on the cell type you will use, such as DMSO or BSA.
For ES cells, we recommend using a concentration of 7.5% to 10% BSA.

Target Background

Function

Ligand for members of the frizzled family of seven transmembrane receptors. Plays an important role in the embryonic development of the urogenital tract and the lung. Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development. Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries. Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary. Required for normal lung development and for normal patterning of trachael cartilage rings.

Gene References into Functions

Annotation of rs2072920 in WNT4 in tissues might be related to obesity. PMID: 28272483
We found a genetic association between rs11031006 (FSHB) SNP and endometriosis. WNT4 and VEZT genes constitute the most consistently associated genes with endometriosis. In the present study, an association of rs7521902 (WNT4) and rs10859871 (VEZT) was confirmed in women with endometriosis at the genotypic but not the allelic level. PMID: 28901453
urinary Wnt4 could be a potential noninvasive biomarker for the early detection of tubular injury. PMID: 27600466
were not able to replicate or further verify the genetic association of polymorphisms in WNT4 and WNT5B with bone mineral density PMID: 28078366
These findings demonstrate that autocrine human growth hormone regulates WNT4 expression and that WNT4 is a potential therapeutic target in human breast cancer. PMID: 27323961
WNT4 encodes wingless-type MMTV integration site family member 4. WNT4 mutations have been found in women with mullerian duct abnormalities, primary amenorrhea, and hyperandrogenism and common variants in WNT4, which are in high linkage disequilibrium with our index SNPs, are associated with endometriosis, ovarian cancer,and bone mineral density of WNT4, and the T allele generates a strong ESR1-binding site. PMID: 28877031
Our MRKH families included 43 quads, 26 trios, and 30 duos. Of our MRKH probands, 87/147 (59%) had MRKH type 1 and 60/147 (41%) had type 2 with additional anomalies. CONCLUSION(S): Although the prevalence of WNT4, HNF1B, and LHX1 point mutations is low in people with MRKH, the prevalence of CNVs was approximately 19%. PMID: 28600106
WNT4 drives a novel signaling pathway in ILC cells, with a critical role in estrogen-induced growth that may also mediate endocrine resistance. WNT4 signaling may represent a novel target to modulate endocrine response specifically for patients with ILC. PMID: 27650553
The etiology of MRKH syndrome is still largely unknown, probably because of its intrinsic heterogeneity. Several candidate causative genes have been investigated, but to date only WNT4 has been associated with MRKH with hyperandrogenism. This review summarizes and discusses the clinical features and details progress to date in understanding the genetics of MRKH syndrome. PMID: 27716927
We highlight the cooperation of WNT4, RSPO1 and FOXL2 within a regulatory network and the need for further research to better understand their role in defining and maintaining ovarian identity. PMID: 27604691
The WNT4 expression level in eutopic endometrium was significantly reduced compared with that in normal endometrium of the control group. PMID: 26363035
Results suggest that the Wnt4 gene encodes signals that are important for various aspects of female reproductive tract development. PMID: 26721931
Studied the roles of WNT4 and WNT5A in human decidulization and their relationship with preeclampsia. PMID: 26848991
Polymorphisms on WNT4 gene might be involved in the pathogenesis of endometriosis in the infertile women. PMID: 26139156
the expression of WNT4, a Wnt ligand, and three targets of Wnt-ss-catenin transcription activation, namely, MMP7, cyclinD1 (CD1) and c-MYC in 141 penile tissue cores from 101 unique samples, were investigated. PMID: 25901368
The results demonstrated that WNT4 rs2235529 is associated with endometriosis in Chinese Han women, which may result in aberrant expression of WNT4, leading to the pathogenesis of endometriosis. PMID: 25682310
Wnt4 might be a key gene during parathyroid hormone-induced epithelial mesenchymal transformation of proximal kidney tubule cells. PMID: 25337242
The 5'-flanking region of the Wnt4 gene is responsive to Pax8. Pax8 modulates the expression of Wnt4 in thyroid cells. PMID: 25270402
Our results suggest that the rs10965235 SNP in the CDKN2B-AS gene and the rs16826658 SNP near the WNT4 gene were significantly associated with endometriosis in this Korean population. PMID: 25154675
We report an adolescent girl with the cardinal features of MURCS association, obesity, and clinical findings of hyperandrogenism who did not show any exonic mutation of the WNT4 gene PMID: 24356390
These data indicate that Wnt4 signaling is deregulated in most pituitary adenomas and its excessive activation may inhibit pituitary tumor invasion. PMID: 24200887
State anxiety assessed two months before the examination was positively and negatively correlated with miR-16 and its target WNT4 mRNA levels, respectively. PMID: 24130753
The WNT4 ligand plays a role in regulating the cell growth of leukemia-derived cells by arresting cells in the G1 cell cycle phase. PMID: 24274766
Analysis of bronchial biopsy samples shows a very strong correlation between Wnt4 and interleukin (IL)8 gene expression, suggesting a role for Wnt4 in chronic lung inflammation. PMID: 23463699
heterozygous WNT4 variants are likely to play a causative role in renal hypodysplasia. PMID: 23520208
We confirm WNT4, CDKN2BAS and FN1 as the first identified common loci for endometriosis. PMID: 23142796
Recent developments have demonstrated that ovarian development is an active process (rather than a default process); ovarian development/function requires expression of WNT4, RSPO1, and FOXL2. [REVIEW] PMID: 23044875
Studies uncovered a linear pathway involving BMP2, WNT4/beta-catenin, and Forkhead box protein O1 that operates in human endometrium to critically control decidualization. PMID: 23142810
Mutations in the WNT4 gene is not associated with premature ovarian failure. PMID: 22951804
Mutations in WNT4 are not responsible for Mullerian duct abnormalities in Chinese women. PMID: 22503279
Wnt/beta-catenin pathway forms a negative feedback loop during TGF-beta1 induced human normal skin fibroblast-to-myofibroblast transition PMID: 22041457
Possible role of WNT4 in Han Chinese women with premature ovarian failure. PMID: 21624127
Report a new WNT4 gene mutation in atypical Mayer-Rokitansky-Kuster-Hauser syndrome. PMID: 21377155
These observations highlight the distinct roles of WNT11 and WNT4 during the early stages of retinoic acid-induced neuronal differentiation. PMID: 21280163
Wnt4 is specifically involved in joint development. PMID: 20709709
Findings provide the first convincing line of evidence that EAF and Wnt4 form an auto-regulatory negative feedback loop in vivo. PMID: 20161747
at least 1 cause of Mayer-Rokitansky-Kuster-Hauser (MRKH) syndrome is a loss of function mutation of the Wnt4 (wingless-type MMTV integration site family member 4) gene. PMID: 20060343
The age-related down-regulation of Wnt4 (and subsequently FoxN1), and the prominent increase in LAP2alpha expression, was investigated in thymic epithelial cells. PMID: 20502698
The current results support a view that WNT4 may have a role in oocyte selection and follicle formation and maturation in human ovaries. PMID: 19962424
Results demonstrated significant up-regulation of WNT-3, WNT-4, WNT-5B, WNT-7B, WNT-9A, WNT-10A, and WNT-16B in patients with CLL compared to normal subjects. PMID: 19863181
Wnt 4a gene encodes a growth factor that participates in the formation of the kidney, adrenal, mammary gland, pituitary, and female reproductive tract. Wnt4 is required for the initial steps of the formation of Mullerian ducts. PMID: 19849868
WNT-4 protein is expressed in the developing nephron during morphogenetic period of the kidney. PMID: 12768078
Down-regulation of Wnt-4 and up-regulation of Wnt-5a are possible markers of the malignant phenotype of human squamous cell carcinoma. PMID: 12841867
overexpression disrupts normal testicular vasculature and inhibits testosterone synthesis by repressing steroidogenic factor 1/beta-catenin synergy PMID: 12949260
matrilysin may have a role in renal tubular injury and progression of tubulointerstitial fibrosis, and Wnt4 may regulate matrilysin expression in the kidney PMID: 15149334
Their different spatial expression patterns suggest that Wnt4 and Wnt5a proteins are not functionally linked to type II collagen and type X collagen synthesis in in vitro chondrogenic models of mesenchyme stem cells PMID: 15389636
sequencing of all five exons of WNT-4 demonstrated no mutant alleles in any of 8 46,XX true hermaphrodites. The possibility of the existence of causative mutations in the untranslated regions of WNT-4, or within introns cannot be ruled out. PMID: 15589122
It is thus likely that both RE1 and RE2 are necessary in rendering p63/p73-specific activation of the WNT4 promoter. PMID: 16343436
Bone morphogenetic protein-2 modulates Wnt and frizzled expression and enhances the canonical pathway of Wnt signaling in normal keratinocytes PMID: 16442268
These data show that DKK3 and WNT4 have multiple actions on steroidogenesis in adrenocortical cells, including effects on overall steroidogenesis (aldosterone and cortisol biosynthesis) and distinct effects on steroidogenic enzyme mRNA levels. PMID: 16981135

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Involvement in disease

46,XX sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL); Mullerian aplasia and hyperandrogenism (MULLAPL)

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Protein Families

Wnt family

Database Links

HGNC: 12783

OMIM: 158330

KEGG: hsa:54361

STRING: 9606.ENSP00000290167

UniGene: Hs.25766

Code	CSB-EP026137HU
Abbreviation	Recombinant Human WNT4 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Protein Wnt-4 (WNT4)

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