Human Vascular Endothelial cell Growth Factor B,VEGF-B ELISA KIT

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Target Name

vascular endothelial growth factor B

Alternative Names

Vascular endothelial growth factor B ELISA Kit; Vascular endothelial growth factor-related factor ELISA Kit; VEGF B ELISA Kit; VEGF related factor ELISA Kit; VEGF-B ELISA Kit; VEGF-related factor ELISA Kit; Vegfb ELISA Kit; VEGFB_HUMAN ELISA Kit; VEGFL ELISA Kit; VRF ELISA Kit

Abbreviation

VEGFB

Uniprot No.

P49765

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-96
1:2	Average %	100
	Range %	95-104
1:4	Average %	99
	Range %	92-110
1:8	Average %	89
	Range %	86-96

Recovery

The recovery of human VEGF-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-105
EDTA plasma (n=4)	96	92-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.697	2.512	2.605	2.463
500	2.322	2.201	2.262	2.120
250	1.546	1.498	1.522	1.380
125	0.815	0.789	0.802	0.660
62.5	0.417	0.410	0.414	0.272
31.2	0.281	0.276	0.279	0.137
15.6	0.219	0.213	0.216	0.074
0	0.143	0.140	0.142

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human VEGFB ELISA Kit was designed for the quantitative measurement of Human VEGFB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Related Products

Target Background

Function
(From Uniprot)

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.

Gene References into Functions

High plasma VEGF-B levels are associated with type 2 diabetes mellitus. PMID: 28523459
Data from clinical studies point out the changes in circulating or tissue expression levels of VEGF-B in obese compared with lean patients. PMID: 28798193
Cardiac transgenic vascular endothelial growth factor-B overexpression failed to protect heart transplants from ischemia-reperfusion injury. PMID: 27588416
renal VEGF-B expression correlates with the severity of Diabetic Kidney Disease. PMID: 28190774
Data show that metformin treatment reduces serum vascular endothelial growth factor B (VEGF-B) levels and ameliorates insulin resistance. PMID: 26387747
Frameshift mutations of VEGFB gene is associated with stomach and colorectal cancers. PMID: 25633991
fluid shear stress induces the synthesis of Insulin growth factor-2 and vascular endothelial growth factor (VEGF) B and D, which in turn transactivate MMP-12. PMID: 25435370
MMP9 may activate VEGF-B via PI3K/Akt signaling pathway. PMID: 25424698
Roles of vascular endothelial growth factor in amyotrophic lateral sclerosis. PMID: 24987705
Low VEGFB and VEGFD gene expression is associated with early-stage non-small cell lung cancer. PMID: 24145997
Our study suggested that VEGF-B was an angiogenesis factor in vitro and that ERK1/2 and p38-related signaling pathways were involved in these VEGF-B activities. PMID: 24374930
VEGF-B has possible roles in cardiac protection, energy metabolism support, and neuroprotectin [review] PMID: 24987005
High VEGF-B levels might correlate with the presence of hyperlipidemia and target organ damage in type 2 diabetic patients. PMID: 25001655
High VEGFB expression is associated with bone marrow metastasis in neuroblastoma. PMID: 23553333
VEGF-B might be an important ligand in the signalling between the tumor and preexisting blood vessels to ensure a functional blood supply for tumor survival. PMID: 23417498
we report significant associations with overall survival and distant failure for certain VEGF(VEGF-B) family members. PMID: 23728940
Data indicate that three miRs (miR-484, -642, and -217) were able to predict chemoresistance and vasculature of serous epithelial ovarian carcinomas through the regulation of the VEGFB and VEGFR2 pathways. PMID: 23697367
Expression of VEGF-B genes in glioma cell lines U87 is significantly changed under hypoxia and ischemic conditions. PMID: 23350126
In WT1 mutant cells, reduced VEGF(165)b was due to lack of WT1-mediated transcriptional repression of the splicing-factor kinase SRPK1 PMID: 22172722
analysis of binding of vascular endothelial growth factor-B by VEGFR-1(D2) PMID: 20501651
TIMP3 blocks the binding of VEGF to VEGF receptor-2 and inhibits downstream signaling and angiogenesis. PMID: 12652295
Results describe the crystal structure of human vascular endothelial growth factor-B (VEGF-B) and present a predicted model for the association of VEGF-B with the second domain of its receptor, VEGFR-1. PMID: 16616187
Basophils could play a role in angiogenesis and inflammation through the expression of several forms of VEGF-B and their receptors. PMID: 17082651
VEGFB, and receptor were highly expressed in dysplastic neurons. IR in astroglial and balloon cells was observed for VEGFA and its receptors Double-labeling also showed expression of VEGFA, VEGFB and VEGFR-1 in cells of the microglia/macrophage lineage. PMID: 18317782
VEGF-B appears to have a relatively restricted angiogenic activity in the ischemic heart. PMID: 18511699
Increased VEGFB expression is associated with hepatocellular carcinoma PMID: 18537151
Overexpression of vascular endothelial growth factor-B in mouse heart alters cardiac lipid metabolism and induces myocardial hypertrophy. PMID: 18757827
The structural features of the 'highly ordered' interaction of the Fab fragment of the antibody (Fab-2H10) with VEGF-B, is presented. PMID: 18930733
VEGF-B mRNA was not expressed either in normal urothelium or in bladder cancer. PMID: 19424629

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Subcellular Location

Secreted. Note=Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

Protein Families

PDGF/VEGF growth factor family

Tissue Specificity

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

Database Links

HGNC: 12681

OMIM: 601398

KEGG: hsa:7423

STRING: 9606.ENSP00000311127

UniGene: Hs.732095

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Code	CSB-E04758h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.