Human granzyme B (GZMB) ELISA Kit

Code CSB-E08718h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human GZMB ELISA Kit was designed for the quantitative measurement of Human GZMB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.

Target Name granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Alternative Names C11 ELISA Kit; Cathepsin G like 1 ELISA Kit; Cathepsin G-like 1 ELISA Kit; CCPI ELISA Kit; CGL 1 ELISA Kit; CGL1 ELISA Kit; CSP B ELISA Kit; CSPB ELISA Kit; CTLA 1 ELISA Kit; CTLA-1 ELISA Kit; CTLA1 ELISA Kit; CTSGL1 ELISA Kit; Cytotoxic serine protease B ELISA Kit; Cytotoxic T lymphocyte associated serine esterase 1 ELISA Kit; Cytotoxic T lymphocyte proteinase 2 ELISA Kit; Cytotoxic T-lymphocyte proteinase 2 ELISA Kit; Fragmentin 2 ELISA Kit; Fragmentin-2 ELISA Kit; GRAB_HUMAN ELISA Kit; Granzyme 2 ELISA Kit; Granzyme B (granzyme 2; cytotoxic T lymphocyte associated serine esterase 1) ELISA Kit; Granzyme B ELISA Kit; Granzyme-2 ELISA Kit; GranzymeB ELISA Kit; GRB ELISA Kit; Gzmb ELISA Kit; Hlp ELISA Kit; Human lymphocyte protein ELISA Kit; Lymphocyte protease ELISA Kit; Protease; serine; B ELISA Kit; SECT ELISA Kit; T cell serine protease 1 3E ELISA Kit; T cell serine protease 1-3E ELISA Kit; T-cell serine protease 1-3E ELISA Kit
Abbreviation GZMB
Uniprot No. P10144
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Gzms-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 89  
Range % 85-93  
1:10 Average % 86  
Range % 83-89  
1:20 Average % 91  
Range % 85-97  
1:40 Average % 93  
Range % 90-96  
Recovery
The recovery of human Gzms-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 99 97-101  
EDTA plasma (n=4) 97 93-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.634 2.698 2.666 2.524  
50 1.913 1.932 1.923 1.781  
25 1.110 1.135 1.123 0.981  
12.5 0.658 0.632 0.645 0.503  
6.25 0.396 0.385 0.391 0.249  
3.12 0.299 0.279 0.289 0.147  
1.56 0.205 0.221 0.213 0.071  
0 0.141 0.143 0.142    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Gene References into Functions
  1. our exploratory study suggests that EOMES, BCL6 and GZMB gene expression are aberrant within the PB T cell transcriptome of HT patients. The association of this transcription signature with the heterogeneity of HT and disease control is suggested. PMID: 29319368
  2. Serum interleukin 1 receptor antagonist (IL1RA) and granzyme B (GZMB) levels were markedly increased in Crohn's disease (CD) patients, suggesting that these markers can serve as biomarkers to identify gut inflammation. PMID: 28972805
  3. Authors found that R(48)-GzmB is a stable protein that accumulates to similar levels in activated NK cells as Q(48)-GzmB. rs8192917 polymorphism may influence antitumor activity and the effect of antitumor cellular immunotherapy. PMID: 28653095
  4. GzmA and gzmB were predominantly expressed by natural killer cells, and during abdominal sepsis, the percentage of these cells expressing gzms in peritoneal lavage fluid decreased, while the amount of expression in the gzm(+) cells increased PMID: 28694562
  5. Study provides evidence that Il10 expression is limited to migratory CD25++ regulatory T cells only. PMID: 27756896
  6. Long-term lung function decline in asthma is associated with elevation of bronchial CD8 and CD4 at baseline, and CD8, CD3 and granzyme B at follow-up PMID: 27230446
  7. Granzyme B breaches the inner membrane through Tim22, the metabolite carrier translocase pore, in a mitochondrial heat-shock protein 70-dependent manner. PMID: 28338658
  8. Differential proteomics were used to identify granzyme B substrates in three unrelated bacteria: Escherichia coli, Listeria monocytogenes, and Mycobacteria tuberculosis. Granzyme B cleaves a highly conserved set of proteins in all three bacteria, which function in vital biosynthetic and metabolic pathways that are critical for bacterial survival under diverse environmental conditions. PMID: 29107333
  9. Proteolysis by granzyme B enhances presentation of autoantigenic PAD4 epitopes in rheumatoid arthritis. PMID: 27700100
  10. our results suggest granzyme B PET imaging can serve as a quantitatively useful predictive biomarker for efficacious responses to cancer immunotherapy. PMID: 28461564
  11. Plasma GzmB levels may reflect the degree of pruritus and dermatitis in patients with atopic dermatitis PMID: 27686401
  12. These results suggest the critical importance of miR-378 in the regulation of GrzB expression and a protective role for GrzB in controlling dengue virus replication in vivo. PMID: 26166761
  13. Analysis of infiltrating granzyme B-expressing T cells at the invasive borders of the colon tumors revealed a significant difference in expression granzyme by race underlining decreased antitumor cytotoxic immunity in African Americans. PMID: 27310868
  14. Increased TIM3+CD8+T cells with lower perforin and granzyme B expression and higher CD95 expression in MDS patients were observed. PMID: 27846431
  15. granzyme-B levels were found to be significantly associated with increased insulin resistance in adolescent polycystic ovary syndrome patients. Additionally, elevated levels of serum granzyme-B were predictive for increased cardiovascular risk in PCOS patients PMID: 26802256
  16. results suggest that enhanced IL-21R expression of CD19(+)CD5(+) B cells and production of IL-21 by iNKT cells may play an important role in the pathogenesis of pSS by regulating CD19(+)CD5(+) B cell functions and increasing GrB production, presumably leading to a counter-regulatory effect in the disease. PMID: 26884645
  17. Findings show that GrB was produced in 57.1% colorectal cancer cell (CRC) lines and 100% CRC-derived Cancer Stem Cells and present a novel role for GrB as up-modulator of epitelial-to-mesenchimal transition in CRC cells. PMID: 26830472
  18. FASL, granzyme B, and cytochrome c blood expression reflects breast cancer progression and response to therapy. (Review) PMID: 27117663
  19. Data show that NK cell lines could secreted rapidly inactive Mr 35 000 granzyme B (GZB)outside secretory lysosomes (SLs). PMID: 26927382
  20. Data suggest that reactive oxygen species (ROS) generated within cytotoxic lymphocytes by receptor stimulation are required for lysosomal permeabilization and release of GzmB (granzyme B) into the cytosol and for inactivation of serpin B9. PMID: 26670609
  21. these results suggest a perforin-independent, extracellular role for GzmB in the pathogenesis of cardiac fibrosis PMID: 26610869
  22. Among SLAMF4+ cells, the T cell fraction positive for perforin and granzyme B was higher in those obtained from healthy donors, while the percentage of T cells that were single-positive for granzyme B was higher in cells obtained from patients with SLE. PMID: 26314831
  23. Costimulation blockade by abatacept can decrease the serum levels of GZMB in rheumatoid arthritis patients responding to the treatment. PMID: 26633185
  24. elevated in the inflammatory lesions of placentas with villitis of unknown etiology PMID: 25725937
  25. Combined evaluation of granzyme B and perforin may have a role in noninvasive diagnosis of acute rejection after kidney transplantation. PMID: 25643139
  26. this study emphasizes that the coordinated action of hGzmB-activated p53 and GzmB-cleaved Bid is important for GzmB-induced cell death and for cytotoxic lymphocyte/Natural Killer Cell-mediated killing of target cells. PMID: 25404359
  27. identified among the key genes in circulating monocytes that were altered by exercise PMID: 26207425
  28. GB-induced ROS significantly promote apoptosis. PMID: 25361078
  29. Active secretion of CXCL10 and CCL5 from colorectal cancer microenvironments associates with granzymeB+ CD8+ T-cell infiltration. PMID: 25671296
  30. Synthetic consensus HIV-1 DNA induces potent cellular immune responses and synthesis of granzyme B, perforin in HIV infected individuals. PMID: 25531694
  31. GzmB plays no role in the pathogenesis of keloids and hypertrophic scars. PMID: 26410968
  32. PFN appears to form arc structures on target membranes that serve as minimally disrupting conduits for GzmB translocation. PMID: 25146929
  33. These results suggest that GZMB Q55R, P94A, and Y247H polymorphisms are not significantly associated with colon cancer incidence, or metastasis to lymph node and distant organ. PMID: 25313744
  34. CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients. PMID: 25780036
  35. Data indicate that treatment with toll-like receptor 8 (TLR8) agonists elicited granzyme B production. PMID: 25667415
  36. The results of study found implicate possible effect of this genetic polymorphism in susceptibility to SSPE which needs to be confirmed in bigger populations. PMID: 24875585
  37. Activated CD4 and CD8 T cells secrete similar amounts of GrzB. PMID: 25245659
  38. The suppression-abrogating cytokine IL-6 augments GzmB expression by human CD4 T cells, and it inhibits Treg suppression via a nonapoptotic GzmB-mediated mechanism. PMID: 25637022
  39. Polarized granzyme release is required for antigen-driven transendothelial migration of human effector memory CD4 T cells. PMID: 25367116
  40. CASP is specifically cleaved by granzymeB. PMID: 25159843
  41. Serum concentrations of granzyme B in patients with ovarian cancer were substantially increased in comparison to concentrations in patients with ovarian cystadenomas or ovarian teratomas. PMID: 24673566
  42. The GrB-Sb9 nexus may therefore represent an additional mechanism of limiting lymphocyte lifespan and populations. PMID: 24488096
  43. our data indicate that the co-expression of perforin and granzyme B genes exhibits anticancer potential PMID: 24696715
  44. Cancer cells released soluble factors that inhibited granzyme B, perforin and IFN-gamma production. PMID: 24894428
  45. multiple linear regression analysis revealed that both type 2 diabetes mellitus and central obesity were predicting factors for GzmB, findings reveal a possible role of GzmB in type 2 diabetes mellitus. PMID: 24195710
  46. cleaves proIL-18 into active form and promotes apoptosis in the inflammed skin PMID: 23820889
  47. These results suggest that GrzB from CCR5+ memory CD4 T cells may have a role in cellular and tissue pathologies during HIV infection. PMID: 24999042
  48. These data indicate that IBD-related inflammation is marked by mucosal accumulation of cytotoxic, GrB-expressing CD19(+) and IgA(+) cells, suggesting a role for these cells in IBD-associated epithelial damage. PMID: 24835396
  49. Report increased granzyme B expression in discoid lupus erythematosus. PMID: 23980805
  50. Unlike IL-18 and FKN, plasma GZB may have a role in severity of acute coronary syndrome PMID: 24307760

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Subcellular Location Secreted. Cytolytic granule.
Protein Families Peptidase S1 family, Granzyme subfamily
Database Links

HGNC: 4709

OMIM: 123910

KEGG: hsa:3002

STRING: 9606.ENSP00000216341

UniGene: Hs.1051

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