Product Details

Uniprot No.

Target Names

Alternative Names

Granzyme B (EC 3.4.21.79) (C11) (CTLA-1) (Cathepsin G-like 1) (CTSGL1) (Cytotoxic T-lymphocyte proteinase 2) (Lymphocyte protease) (Fragmentin-2) (Granzyme-2) (Human lymphocyte protein) (HLP) (SECT) (T-cell serine protease 1-3E), GZMB, CGL1 CSPB CTLA1 GRB

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human Granzyme B

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6A1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Granzyme B is a serine protease that plays a central role in immune-mediated cell death, functioning as the primary effector molecule through which cytotoxic T lymphocytes and natural killer cells eliminate virus-infected and transformed cells. By cleaving specific substrates within target cells, Granzyme B initiates apoptotic cascades that are fundamental to immune surveillance and homeostasis. Understanding Granzyme B expression and localization is essential for researchers investigating cytotoxic immune responses, tumor immunology, and inflammatory conditions.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human Granzyme B, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than hybridoma supernatants, researchers can expect reliable performance across experiments and between lots, eliminating a common source of variability in longitudinal studies.

Validation studies demonstrate robust performance in both immunohistochemistry and immunofluorescence applications. In paraffin-embedded human tonsil tissue, the antibody produces clear staining at 1:100 dilution using citrate buffer antigen retrieval, making it well-suited for examining Granzyme B-positive immune cell populations in archival clinical specimens. Immunofluorescence analysis in HeLa cells at 1:50 dilution confirms utility for subcellular localization studies in cultured cells, with the antibody performing effectively following formaldehyde fixation and Triton X-100 permeabilization.

The unconjugated format provides flexibility for researchers to pair this antibody with their preferred detection systems across ELISA, immunohistochemistry, and immunofluorescence platforms. For investigators studying cytotoxic lymphocyte biology, tumor microenvironment characterization, or immune checkpoint mechanisms, this antibody delivers the specificity and batch consistency needed for meaningful experimental outcomes.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Gene References into Functions

our exploratory study suggests that EOMES, BCL6 and GZMB gene expression are aberrant within the PB T cell transcriptome of HT patients. The association of this transcription signature with the heterogeneity of HT and disease control is suggested. PMID: 29319368
Serum interleukin 1 receptor antagonist (IL1RA) and granzyme B (GZMB) levels were markedly increased in Crohn's disease (CD) patients, suggesting that these markers can serve as biomarkers to identify gut inflammation. PMID: 28972805
Authors found that R(48)-GzmB is a stable protein that accumulates to similar levels in activated NK cells as Q(48)-GzmB. rs8192917 polymorphism may influence antitumor activity and the effect of antitumor cellular immunotherapy. PMID: 28653095
GzmA and gzmB were predominantly expressed by natural killer cells, and during abdominal sepsis, the percentage of these cells expressing gzms in peritoneal lavage fluid decreased, while the amount of expression in the gzm(+) cells increased PMID: 28694562
Study provides evidence that Il10 expression is limited to migratory CD25++ regulatory T cells only. PMID: 27756896
Long-term lung function decline in asthma is associated with elevation of bronchial CD8 and CD4 at baseline, and CD8, CD3 and granzyme B at follow-up PMID: 27230446
Granzyme B breaches the inner membrane through Tim22, the metabolite carrier translocase pore, in a mitochondrial heat-shock protein 70-dependent manner. PMID: 28338658
Differential proteomics were used to identify granzyme B substrates in three unrelated bacteria: Escherichia coli, Listeria monocytogenes, and Mycobacteria tuberculosis. Granzyme B cleaves a highly conserved set of proteins in all three bacteria, which function in vital biosynthetic and metabolic pathways that are critical for bacterial survival under diverse environmental conditions. PMID: 29107333
Proteolysis by granzyme B enhances presentation of autoantigenic PAD4 epitopes in rheumatoid arthritis. PMID: 27700100
our results suggest granzyme B PET imaging can serve as a quantitatively useful predictive biomarker for efficacious responses to cancer immunotherapy. PMID: 28461564
Plasma GzmB levels may reflect the degree of pruritus and dermatitis in patients with atopic dermatitis PMID: 27686401
These results suggest the critical importance of miR-378 in the regulation of GrzB expression and a protective role for GrzB in controlling dengue virus replication in vivo. PMID: 26166761
Analysis of infiltrating granzyme B-expressing T cells at the invasive borders of the colon tumors revealed a significant difference in expression granzyme by race underlining decreased antitumor cytotoxic immunity in African Americans. PMID: 27310868
Increased TIM3+CD8+T cells with lower perforin and granzyme B expression and higher CD95 expression in MDS patients were observed. PMID: 27846431
granzyme-B levels were found to be significantly associated with increased insulin resistance in adolescent polycystic ovary syndrome patients. Additionally, elevated levels of serum granzyme-B were predictive for increased cardiovascular risk in PCOS patients PMID: 26802256
results suggest that enhanced IL-21R expression of CD19(+)CD5(+) B cells and production of IL-21 by iNKT cells may play an important role in the pathogenesis of pSS by regulating CD19(+)CD5(+) B cell functions and increasing GrB production, presumably leading to a counter-regulatory effect in the disease. PMID: 26884645
Findings show that GrB was produced in 57.1% colorectal cancer cell (CRC) lines and 100% CRC-derived Cancer Stem Cells and present a novel role for GrB as up-modulator of epitelial-to-mesenchimal transition in CRC cells. PMID: 26830472
FASL, granzyme B, and cytochrome c blood expression reflects breast cancer progression and response to therapy. (Review) PMID: 27117663
Data show that NK cell lines could secreted rapidly inactive Mr 35 000 granzyme B (GZB)outside secretory lysosomes (SLs). PMID: 26927382
Data suggest that reactive oxygen species (ROS) generated within cytotoxic lymphocytes by receptor stimulation are required for lysosomal permeabilization and release of GzmB (granzyme B) into the cytosol and for inactivation of serpin B9. PMID: 26670609
these results suggest a perforin-independent, extracellular role for GzmB in the pathogenesis of cardiac fibrosis PMID: 26610869
Among SLAMF4+ cells, the T cell fraction positive for perforin and granzyme B was higher in those obtained from healthy donors, while the percentage of T cells that were single-positive for granzyme B was higher in cells obtained from patients with SLE. PMID: 26314831
Costimulation blockade by abatacept can decrease the serum levels of GZMB in rheumatoid arthritis patients responding to the treatment. PMID: 26633185
elevated in the inflammatory lesions of placentas with villitis of unknown etiology PMID: 25725937
Combined evaluation of granzyme B and perforin may have a role in noninvasive diagnosis of acute rejection after kidney transplantation. PMID: 25643139
this study emphasizes that the coordinated action of hGzmB-activated p53 and GzmB-cleaved Bid is important for GzmB-induced cell death and for cytotoxic lymphocyte/Natural Killer Cell-mediated killing of target cells. PMID: 25404359
identified among the key genes in circulating monocytes that were altered by exercise PMID: 26207425
GB-induced ROS significantly promote apoptosis. PMID: 25361078
Active secretion of CXCL10 and CCL5 from colorectal cancer microenvironments associates with granzymeB+ CD8+ T-cell infiltration. PMID: 25671296
Synthetic consensus HIV-1 DNA induces potent cellular immune responses and synthesis of granzyme B, perforin in HIV infected individuals. PMID: 25531694
GzmB plays no role in the pathogenesis of keloids and hypertrophic scars. PMID: 26410968
PFN appears to form arc structures on target membranes that serve as minimally disrupting conduits for GzmB translocation. PMID: 25146929
These results suggest that GZMB Q55R, P94A, and Y247H polymorphisms are not significantly associated with colon cancer incidence, or metastasis to lymph node and distant organ. PMID: 25313744
CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients. PMID: 25780036
Data indicate that treatment with toll-like receptor 8 (TLR8) agonists elicited granzyme B production. PMID: 25667415
The results of study found implicate possible effect of this genetic polymorphism in susceptibility to SSPE which needs to be confirmed in bigger populations. PMID: 24875585
Activated CD4 and CD8 T cells secrete similar amounts of GrzB. PMID: 25245659
The suppression-abrogating cytokine IL-6 augments GzmB expression by human CD4 T cells, and it inhibits Treg suppression via a nonapoptotic GzmB-mediated mechanism. PMID: 25637022
Polarized granzyme release is required for antigen-driven transendothelial migration of human effector memory CD4 T cells. PMID: 25367116
CASP is specifically cleaved by granzymeB. PMID: 25159843
Serum concentrations of granzyme B in patients with ovarian cancer were substantially increased in comparison to concentrations in patients with ovarian cystadenomas or ovarian teratomas. PMID: 24673566
The GrB-Sb9 nexus may therefore represent an additional mechanism of limiting lymphocyte lifespan and populations. PMID: 24488096
our data indicate that the co-expression of perforin and granzyme B genes exhibits anticancer potential PMID: 24696715
Cancer cells released soluble factors that inhibited granzyme B, perforin and IFN-gamma production. PMID: 24894428
multiple linear regression analysis revealed that both type 2 diabetes mellitus and central obesity were predicting factors for GzmB, findings reveal a possible role of GzmB in type 2 diabetes mellitus. PMID: 24195710
cleaves proIL-18 into active form and promotes apoptosis in the inflammed skin PMID: 23820889
These results suggest that GrzB from CCR5+ memory CD4 T cells may have a role in cellular and tissue pathologies during HIV infection. PMID: 24999042
These data indicate that IBD-related inflammation is marked by mucosal accumulation of cytotoxic, GrB-expressing CD19(+) and IgA(+) cells, suggesting a role for these cells in IBD-associated epithelial damage. PMID: 24835396
Report increased granzyme B expression in discoid lupus erythematosus. PMID: 23980805
Unlike IL-18 and FKN, plasma GZB may have a role in severity of acute coronary syndrome PMID: 24307760

Hide All

Subcellular Location

Secreted. Cytolytic granule.

Protein Families

Peptidase S1 family, Granzyme subfamily

Database Links

HGNC: 4709

OMIM: 123910

KEGG: hsa:3002

STRING: 9606.ENSP00000216341

UniGene: Hs.1051

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-RA794900A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA794900A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela Cells with CSB-RA794900A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Have Questions?	Leave a Message or Start an on-line Chat

GZMB Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background