Recombinant Human Granzyme B(GZMB)

Code CSB-EP010082HU
Size US$1726
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names GZMB
Uniprot No. P10144
Research Area Immunology
Alternative Names C11; Cathepsin G like 1; Cathepsin G-like 1; CCPI; CGL 1; CGL1; CSP B; CSPB; CTLA 1; CTLA-1; CTLA1; CTSGL1; Cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; Cytotoxic T lymphocyte proteinase 2; Cytotoxic T-lymphocyte proteinase 2; Fragmentin 2; Fragmentin-2; GRAB_HUMAN; Granzyme 2; Granzyme B (granzyme 2; cytotoxic T lymphocyte associated serine esterase 1); Granzyme B; Granzyme-2; GranzymeB; GRB; Gzmb; Hlp; Human lymphocyte protein; Lymphocyte protease; Protease; serine; B; SECT; T cell serine protease 1 3E; T cell serine protease 1-3E; T-cell serine protease 1-3E
Species Homo sapiens (Human)
Source E.coli
Expression Region 21-247aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 31.0 kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Gene References into Functions
  1. our exploratory study suggests that EOMES, BCL6 and GZMB gene expression are aberrant within the PB T cell transcriptome of HT patients. The association of this transcription signature with the heterogeneity of HT and disease control is suggested. PMID: 29319368
  2. Serum interleukin 1 receptor antagonist (IL1RA) and granzyme B (GZMB) levels were markedly increased in Crohn's disease (CD) patients, suggesting that these markers can serve as biomarkers to identify gut inflammation. PMID: 28972805
  3. Authors found that R(48)-GzmB is a stable protein that accumulates to similar levels in activated NK cells as Q(48)-GzmB. rs8192917 polymorphism may influence antitumor activity and the effect of antitumor cellular immunotherapy. PMID: 28653095
  4. GzmA and gzmB were predominantly expressed by natural killer cells, and during abdominal sepsis, the percentage of these cells expressing gzms in peritoneal lavage fluid decreased, while the amount of expression in the gzm(+) cells increased PMID: 28694562
  5. Study provides evidence that Il10 expression is limited to migratory CD25++ regulatory T cells only. PMID: 27756896
  6. Long-term lung function decline in asthma is associated with elevation of bronchial CD8 and CD4 at baseline, and CD8, CD3 and granzyme B at follow-up PMID: 27230446
  7. Granzyme B breaches the inner membrane through Tim22, the metabolite carrier translocase pore, in a mitochondrial heat-shock protein 70-dependent manner. PMID: 28338658
  8. Differential proteomics were used to identify granzyme B substrates in three unrelated bacteria: Escherichia coli, Listeria monocytogenes, and Mycobacteria tuberculosis. Granzyme B cleaves a highly conserved set of proteins in all three bacteria, which function in vital biosynthetic and metabolic pathways that are critical for bacterial survival under diverse environmental conditions. PMID: 29107333
  9. Proteolysis by granzyme B enhances presentation of autoantigenic PAD4 epitopes in rheumatoid arthritis. PMID: 27700100
  10. our results suggest granzyme B PET imaging can serve as a quantitatively useful predictive biomarker for efficacious responses to cancer immunotherapy. PMID: 28461564
  11. Plasma GzmB levels may reflect the degree of pruritus and dermatitis in patients with atopic dermatitis PMID: 27686401
  12. These results suggest the critical importance of miR-378 in the regulation of GrzB expression and a protective role for GrzB in controlling dengue virus replication in vivo. PMID: 26166761
  13. Analysis of infiltrating granzyme B-expressing T cells at the invasive borders of the colon tumors revealed a significant difference in expression granzyme by race underlining decreased antitumor cytotoxic immunity in African Americans. PMID: 27310868
  14. Increased TIM3+CD8+T cells with lower perforin and granzyme B expression and higher CD95 expression in MDS patients were observed. PMID: 27846431
  15. granzyme-B levels were found to be significantly associated with increased insulin resistance in adolescent polycystic ovary syndrome patients. Additionally, elevated levels of serum granzyme-B were predictive for increased cardiovascular risk in PCOS patients PMID: 26802256
  16. results suggest that enhanced IL-21R expression of CD19(+)CD5(+) B cells and production of IL-21 by iNKT cells may play an important role in the pathogenesis of pSS by regulating CD19(+)CD5(+) B cell functions and increasing GrB production, presumably leading to a counter-regulatory effect in the disease. PMID: 26884645
  17. Findings show that GrB was produced in 57.1% colorectal cancer cell (CRC) lines and 100% CRC-derived Cancer Stem Cells and present a novel role for GrB as up-modulator of epitelial-to-mesenchimal transition in CRC cells. PMID: 26830472
  18. FASL, granzyme B, and cytochrome c blood expression reflects breast cancer progression and response to therapy. (Review) PMID: 27117663
  19. Data show that NK cell lines could secreted rapidly inactive Mr 35 000 granzyme B (GZB)outside secretory lysosomes (SLs). PMID: 26927382
  20. Data suggest that reactive oxygen species (ROS) generated within cytotoxic lymphocytes by receptor stimulation are required for lysosomal permeabilization and release of GzmB (granzyme B) into the cytosol and for inactivation of serpin B9. PMID: 26670609
  21. these results suggest a perforin-independent, extracellular role for GzmB in the pathogenesis of cardiac fibrosis PMID: 26610869
  22. Among SLAMF4+ cells, the T cell fraction positive for perforin and granzyme B was higher in those obtained from healthy donors, while the percentage of T cells that were single-positive for granzyme B was higher in cells obtained from patients with SLE. PMID: 26314831
  23. Costimulation blockade by abatacept can decrease the serum levels of GZMB in rheumatoid arthritis patients responding to the treatment. PMID: 26633185
  24. elevated in the inflammatory lesions of placentas with villitis of unknown etiology PMID: 25725937
  25. Combined evaluation of granzyme B and perforin may have a role in noninvasive diagnosis of acute rejection after kidney transplantation. PMID: 25643139
  26. this study emphasizes that the coordinated action of hGzmB-activated p53 and GzmB-cleaved Bid is important for GzmB-induced cell death and for cytotoxic lymphocyte/Natural Killer Cell-mediated killing of target cells. PMID: 25404359
  27. identified among the key genes in circulating monocytes that were altered by exercise PMID: 26207425
  28. GB-induced ROS significantly promote apoptosis. PMID: 25361078
  29. Active secretion of CXCL10 and CCL5 from colorectal cancer microenvironments associates with granzymeB+ CD8+ T-cell infiltration. PMID: 25671296
  30. Synthetic consensus HIV-1 DNA induces potent cellular immune responses and synthesis of granzyme B, perforin in HIV infected individuals. PMID: 25531694
  31. GzmB plays no role in the pathogenesis of keloids and hypertrophic scars. PMID: 26410968
  32. PFN appears to form arc structures on target membranes that serve as minimally disrupting conduits for GzmB translocation. PMID: 25146929
  33. These results suggest that GZMB Q55R, P94A, and Y247H polymorphisms are not significantly associated with colon cancer incidence, or metastasis to lymph node and distant organ. PMID: 25313744
  34. CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients. PMID: 25780036
  35. Data indicate that treatment with toll-like receptor 8 (TLR8) agonists elicited granzyme B production. PMID: 25667415
  36. The results of study found implicate possible effect of this genetic polymorphism in susceptibility to SSPE which needs to be confirmed in bigger populations. PMID: 24875585
  37. Activated CD4 and CD8 T cells secrete similar amounts of GrzB. PMID: 25245659
  38. The suppression-abrogating cytokine IL-6 augments GzmB expression by human CD4 T cells, and it inhibits Treg suppression via a nonapoptotic GzmB-mediated mechanism. PMID: 25637022
  39. Polarized granzyme release is required for antigen-driven transendothelial migration of human effector memory CD4 T cells. PMID: 25367116
  40. CASP is specifically cleaved by granzymeB. PMID: 25159843
  41. Serum concentrations of granzyme B in patients with ovarian cancer were substantially increased in comparison to concentrations in patients with ovarian cystadenomas or ovarian teratomas. PMID: 24673566
  42. The GrB-Sb9 nexus may therefore represent an additional mechanism of limiting lymphocyte lifespan and populations. PMID: 24488096
  43. our data indicate that the co-expression of perforin and granzyme B genes exhibits anticancer potential PMID: 24696715
  44. Cancer cells released soluble factors that inhibited granzyme B, perforin and IFN-gamma production. PMID: 24894428
  45. multiple linear regression analysis revealed that both type 2 diabetes mellitus and central obesity were predicting factors for GzmB, findings reveal a possible role of GzmB in type 2 diabetes mellitus. PMID: 24195710
  46. cleaves proIL-18 into active form and promotes apoptosis in the inflammed skin PMID: 23820889
  47. These results suggest that GrzB from CCR5+ memory CD4 T cells may have a role in cellular and tissue pathologies during HIV infection. PMID: 24999042
  48. These data indicate that IBD-related inflammation is marked by mucosal accumulation of cytotoxic, GrB-expressing CD19(+) and IgA(+) cells, suggesting a role for these cells in IBD-associated epithelial damage. PMID: 24835396
  49. Report increased granzyme B expression in discoid lupus erythematosus. PMID: 23980805
  50. Unlike IL-18 and FKN, plasma GZB may have a role in severity of acute coronary syndrome PMID: 24307760
  51. impaired balance between granzyme B mediated cytotoxicity and its inhibition by protease inhibitor 9 forms an important factor in the pathogenesis of villous atrophy in celiac disease. PMID: 24355225
  52. The precise role of GrzB in plasma cell biology remains to be understood and studies in mice will not help as their plasma cells do not express GrzB. PMID: 24114594
  53. The apoptotic cell-in-cell death occurred only in internalized immune killer cells expressing granzyme B PMID: 24113190
  54. GZMB polymorphism is not associated with graft rejection in kidney transplant recipients. PMID: 23528102
  55. A robust correlation was found between protease inhibitor-9 expression levels and suppression of granzyme B activity in leukemia cells. PMID: 23892923
  56. Data indicate that hypoxia impairs breast cancer cell susceptibility to NK-mediated lysis was related to the degradation of NK-derived granzyme B in autophagosomes. PMID: 24101526
  57. we conclude that in LTRs HCMV-specific CD8+ T cell responses, comprised not only of IFN-g secretion but also of Grz-B release, are essential for sufficient control of HCMV replication. PMID: 23607435
  58. Hantavirus nucleocapsid protein inhibits the enzymatic activity of both granzyme B and caspase 3 thereby protecting infected cells from cytotoxic lymphocytes. PMID: 23555267
  59. We found that blockade of Kv1.3 with both lentivirus and pharmacologic agents effectively inhibited cytotoxic effector memory cells' proliferation, secretion of GrB, and their ability to kill neural progenitor cells. PMID: 23382885
  60. Granzyme B cleavage of fibronectin disrupts endothelial cell adhesion, migration and capillary tube formation. PMID: 23228447
  61. Our results reveal a dichotomy in human CL: CD8(+) granzyme B(+)T cells mediate tissue injury, whereas CD4(+)IFN-gamma(+)T cells mediate leishmania parasite killing. PMID: 23321919
  62. Vitiligo is associated with 55R-94A-247H variant haplotype of GZMB. PMID: 23321921
  63. Granzyme A produced by gamma(9)delta(2) T cells induces human macrophages to inhibit growth of an intracellular pathogen. PMID: 23326234
  64. IL-21-induced granzyme B production by plasmacytoid dendritic cells inhibits T-cell proliferation. PMID: 23407551
  65. These findings identify IL-27-induced IL-21 as a key autocrine regulator of granzyme B expression in human CD8(+) T cells. PMID: 22430249
  66. This study defines the final sequence of events controlling cytotoxic lymphocyte immune defense, in which perforin pores assemble on the target cell plasma membrane, ensuring efficient delivery of lethal granzymes. PMID: 23377437
  67. results suggest a mechanism for how ATP increases TrkH activity by inducing conformational changes in TrkA PMID: 23598339
  68. Single nucleotide polymorphism rs8192916 located in GZMB is associated with the progression of joint destruction in rheumatoid arthritis as well as with RNA expression in whole blood. PMID: 23440692
  69. Common variable immunodeficiency patients with autoimmunity have elevated levels of activated T cells expressing granzyme B and HLA-DR. PMID: 23172556
  70. Data show that upregulation of sCD30, iATP, granzyme B, perforin, and downregulation of HLA-G5 could provide valuable diagnostic standards to identify those recipients in the risk of acute rejection (AR). PMID: 22759804
  71. Patients with chronic immune thrombocytopenia have elevated serum granzyme B levels. PMID: 22476618
  72. lung cancer cells utilise their increased PI-9 expression to protect from granzyme B-mediated cytotoxicity as an immune evasion mechanism PMID: 22387007
  73. Data show that granzyme B (GrB) liberated TGF-beta1 from substrates decorin, biglycan and betaglycan. PMID: 22479366
  74. During chronic inflammation, GATA-3+ T helper (Th)2 inhibitory cells express high levels of inhibitory proteins including interleukin (IL)-10, CTLA-4, and granzyme B, but do so independently of Foxp3. PMID: 22547705
  75. following GzmB uptake, Plasmodium falciparum-infected erythrocytes undergo eryptosis via a perforin-independent, GzmB-mediated mechanism. PMID: 22438997
  76. Inhibition of Granzyme B by PI-9 protects prostate cancer cells from apoptosis. PMID: 21919028
  77. results suggest that cytotoxic T-lymphocytes are activated in severe sepsis and express significantly higher intracellular levels of GzmB and that GzmA and B levels correlate with disease severity. PMID: 22089193
  78. Increased transcript levels of TNF-alpha, TGF-beta, and granzyme B in endomyocardial biopsies correlate with allograft rejection. PMID: 22142046
  79. Granzyme B-induced inhibition of viral translation was independent of the activation of caspases. Eukaryotic initiation factor 4 gamma 3 is newly identified as GrB's target. PMID: 22194691
  80. Cumulative exposure to CD8+ granzyme Bhi T cells is associated with reduced lung function early after lung transplantation. PMID: 22172867
  81. Perforin and granzyme B expression in oral and cutaneous lichen planus PMID: 22050094
  82. association between the granzyme B genotype and better clinical outcomes in patients with myeloid malignancies after unrelated bone marrow transplantation PMID: 21886827
  83. Granzyme B-deficient mice, and to a lesser extent perforin-deficient mice, exhibit a significant increase in the number of antigen-specific CD8+ T cells in the lungs and draining lymph nodes of virally infected animals. PMID: 22084442
  84. Human neurons are selectively susceptible to serine-protease granzyme B (GrB) isolated from cytotoxic T cell granules: activated T cells from GrB knockout mice fail to kill mouse neurons. PMID: 21964027
  85. The present data suggest a new form of interaction between the complement and the apoptosis system on the level of granzyme B that is capable to generate C3a and C5a independently of the established complement proteases. PMID: 21948366
  86. Human microRNA (miR)-27a* is a negative regulator of NK-cell cytotoxicity by silencing Prf1 and GzmB expression. PMID: 21960590
  87. Low expression of Granzyme B was associated with early signs of metastasis in colorectal cancer. PMID: 21884199
  88. cleavage of Notch1 by GrB resulted in a loss of transcriptional activity, independent of Notch1 activation. We conclude that GrB disables Notch1 function, probably resulting in anti-cellular proliferation and cell death signals. PMID: 21548883
  89. that excessive secretion of soluble granzyme B and enhanced activity of cytotoxic T lymphocyte may play a vital role in the pathogenesis of systemic lupus erythematosus and organ damage PMID: 21458778
  90. Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein PMID: 21203542
  91. Our data suggest that granzyme B may exert cell death through DNA damage signaling. PMID: 21301799
  92. elevated mRNA levels in broncho-alveolar-lavage, but not in blood, during acute rejection of lung allografts PMID: 20633650
  93. JunB is a direct transcriptional activator of GzB and that GzB transcription is also promoted by NPM-ALK. PMID: 21326808
  94. granzyme B gene expression and therefore human NK-cell cytotoxicity can be regulated by the PP2A-SET interplay PMID: 21156847
  95. Upregulation of granzyme B is associated with acute pancreas allograft rejection. PMID: 20497195
  96. Results showed high concentrations of granzyme B-positive cells both in the epithelium and in the whole mucosa of chronic graft vs host disease patients; however, apoptosis seems to correlate with granzyme release in oral lichen planus patients. PMID: 20618609
  97. These results suggest that increased leukocyte infiltration and elevated Gra-b levels in the post-stroke brain can induce contact-dependent and independent post-ischemic neuronal death to aggravate stroke injury. PMID: 20825413
  98. The gene structure and expression of GrB, its biosynthesis and activation, delivery mechanisms into the target cell cytoplasm, direct proteolytic involvement in activation of several pro-apoptotic pathways, and regulation of its activity in cancer cells. PMID: 21042704
  99. elevated serum level associated with systemic lupus erythematosus PMID: 20394077
  100. a clathrin-dependent endocytosis stimulated by target cell occurs in NK92 cell line, which is closely correlated with granzyme B recovery. PMID: 20449762

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Subcellular Location Cytoplasmic granule
Protein Families Peptidase S1 family, Granzyme subfamily
Database Links

HGNC: 4709

OMIM: 123910

KEGG: hsa:3002

STRING: 9606.ENSP00000216341

UniGene: Hs.1051

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