Human heme oxygenase 1,HO-1 ELISA Kit

Code CSB-E08266h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P09601
Lead Time 3-5 working days
Abbreviation HMOX1
Target Name heme oxygenase (decycling) 1
Alias HO-1, HSP32, bK286B10, heme oxygenase (decyclizing) 1
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
HGNC 5013
RGD 2806
MGI 96163
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %80-94
1:2Average %95
Range %89-99
1:4Average %98
Range %92-105
1:8Average %94
Range %88-99
Recovery
The recovery of human HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8985-92
EDTA plasma (n=4)9890-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
10002.449 2.284 2.367 2.263
5001.967 1.864 1.916 1.812
2501.277 1.233 1.255 1.151
1250.701 0.674 0.688 0.584
62.50.430 0.421 0.426 0.322
31.20.258 0.253 0.256 0.152
15.60.187 0.184 0.186 0.082
00.104 0.103 0.104  
References
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Function Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Involvement in disease Heme oxygenase 1 deficiency (HMOX1D)
Subcellular Location Microsome, Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side
Protein Families Heme oxygenase family
Tissue Specificity Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).
Database Links

HGNC: 5013

OMIM: 141250

KEGG: hsa:3162

STRING: 9606.ENSP00000216117

UniGene: Hs.517581

Pathway HIF-1 signaling pathway

Q&A and Customer Reviews

Q&A
Q:

I want to measure serum levels of human heme oxygenase 1 (HO-1) in newborn. Your product (Uniprot No. P09601) is suitable for this purpose?
Its Code is CSB-E08266h.

A:
Thanks for your inquiry.
We tested healthy human serum or plasma samples with CSB-E08266h before.It can be used to detect heme oxygenase 1 in newborn.But the results might be different.Pls follow your actual test results.
Customer Reviews

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