Human heme oxygenase 1,HO-1 ELISA Kit

Human heme oxygenase 1,HO-1 ELISA Kit

Q&A and Customer Reviews

Related Products

Most popular with customers

CloseMessage

Specification

Target

HMOX1 Antibodies

HMOX1 Proteins

Your Good Partner in Biology Research

Tel : 301-363-4651

Home
Kit
ELISA Kit
Human heme oxygenase 1,HO-1 ELISA Kit

Specification
Target

Code

CSB-E08266h

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P09601

Lead Time

3-5 working days

Abbreviation

HMOX1

Target Name

heme oxygenase (decycling) 1

Alias

HO-1, HSP32, bK286B10, heme oxygenase (decyclizing) 1

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 ng/mL-1000 ng/mL

Sensitivity

3.9 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.

HGNC

5013

RGD

2806

MGI

96163

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-94
1:2	Average %	95
	Range %	89-99
1:4	Average %	98
	Range %	92-105
1:8	Average %	94
	Range %	88-99

Recovery

The recovery of human HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-92
EDTA plasma (n=4)	98	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.449	2.284	2.367	2.263
500	1.967	1.864	1.916	1.812
250	1.277	1.233	1.255	1.151
125	0.701	0.674	0.688	0.584
62.5	0.430	0.421	0.426	0.322
31.2	0.258	0.253	0.256	0.152
15.6	0.187	0.184	0.186	0.082
0	0.104	0.103	0.104

References

VEGFR-1, Bcl-2, and HO-1 Ratios in Pregnant Women With Hypertension Varol F et al,Clin Appl Thromb Hemost,Aug 7.
Embryotoxicity Caused by DON-Induced Oxidative Stress Mediated by Nrf2/HO-1 Pathway Miao Yu.et al,Toxins (Basel),Jun 9
Hypoxia modulates the antioxidant effect of hydroxytyrosol in MCF-7 breast cancer cells Jesús Calahorra.et al,PLOS ONE,September 20
Serum heme oxygenase 1 (HO-1), soluble FMS like tyrosine kinase (sFlt-1) level, and neonatal outcome in early onset, late onset preeclampsia, and normal pregnancy Aldika Akbar MI.et al,hypertension in pregnancy,Octobert 2

Still Have Questions?

Leave a Message or Start an on-line Chat

Function	Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Involvement in disease	Heme oxygenase 1 deficiency (HMOX1D)
Subcellular Location	Microsome, Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side
Protein Families	Heme oxygenase family
Tissue Specificity	Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).
Database Links	HGNC: 5013 OMIM: 141250 KEGG: hsa:3162 STRING: 9606.ENSP00000216117 UniGene: Hs.517581
Pathway	HIF-1 signaling pathway

Q&A

I want to measure serum levels of human heme oxygenase 1 (HO-1) in newborn. Your product (Uniprot No. P09601) is suitable for this purpose?
Its Code is CSB-E08266h.

Thanks for your inquiry.
We tested healthy human serum or plasma samples with CSB-E08266h before.It can be used to detect heme oxygenase 1 in newborn.But the results might be different.Pls follow your actual test results.

Hide All

Customer Reviews

Submit CUSABIO Product Reciew in 2 Minutes, Win An Apple Watch!

HMOX1 Antibody

HMOX1 Protein

HMOX1 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA010583GA01HU	HMOX1 Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA010583LC01HU	HMOX1 Antibody, FITC conjugated	Human	ELISA
CSB-PA010583LB01HU	HMOX1 Antibody, HRP conjugated	Human	ELISA
CSB-PA010583LD01HU	HMOX1 Antibody, Biotin conjugated	Human	ELISA
CSB-PA097056	HMOX1 Antibody	Human,Mouse,Rat	ELISA,IHC

HMOX1 Antibodies for Human

Code	Product Name	Species Reactivity	Application
CSB-PA010583LA01HU	HMOX1 Antibody	Human	ELISA, IHC, IF

HMOX1 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP010583HU CSB-BP010583HU CSB-MP010583HU	Recombinant Human Heme oxygenase 1(HMOX1)	Yeast Baculovirus Mammalian cell
CSB-EP010583HU	Recombinant Human Heme oxygenase 1(HMOX1),partial	E.coli

Human 25-hydroxy vitamin D3,25HVD3 ELISA Kit

Detect Range: 20 μg/L-100 μg/L

Sensitivity: 5 μg/L
Rat 25-hydroxy vitamin D3(25 HVD3)ELISA Kit

Detect Range: 20 μg/L-100 μg/L

Sensitivity: 5 μg/L
Human α2 macroglobulin,α2 MG ELISA Kit

Detect Range: 1.25 μg/mL-20 μg/mL

Sensitivity: 1.25 μg/mL
Human Arachidonic Acid,AA ELISA Kit

Detect Range: 78.125 ng/mL-5000 ng/mL

Sensitivity: 69.748 ng/mL
Rat Arachidonic Acid(AA) ELISA Kit

Detect Range: 3.125 ng/mL-200 ng/mL

Sensitivity: 2.758 ng/mL
Hamster adiponectin, C1Q and collagen domain containing (ADIPOQ)ELISA kit

Detect Range: 0.469 ng/mL-30 ng/mL

Sensitivity: 0.117 ng/mL
Human adiponectin,ADP ELISA Kit

Detect Range: 1.562 ng/mL-100 ng/mL

Sensitivity: 1.102 ng/mL
Mouse adiponectin,ADP ELISA Kit

Detect Range: 0.156 ng/mL-10 ng/mL

Sensitivity: 0.039 ng/mL

Product Center

Kit

Protein

Antibody

Molecular Biology Product

Small Molecule

ELISA kit FAQs Four Types of ELISA 11 Tips for Choosing Your Right ELISA Kit How to Do The Pre-experiment before Your ELISA Experiment? ELISA Data Analysis Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

Promotions

Products
Service
Technical Resources
Research Tools
About Us
Contact Us
- CUSABIO TECHNOLOGY LLC
- Tel: 301-363-4651
- Email: support@cusabio.com
Join Us with

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Product >> Product Name：
Product >> Code：
Product >> Size：
Name： *
Email： *
Phone：
We recommend you to leave your phone number because occasionally our emails get flagged by spam filters. We would not disclose any of your private information to any third party.
Question/Comment： *
Your Region： *
Country State：
I'd like to receive email newsletters from Cusabio

Yes

No
How did you know this promotion?

Email

Distributor

Search engine

Advertisement

Others
Check Code：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.