Human heme oxygenase 1,HO-1 ELISA Kit

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Target Background

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Name: Human heme oxygenase 1,HO-1 ELISA Kit
Brand: CUSABIO
SKU: CSB-E08266h

Product Details

Description

The human HO-1 ELISA Kit is engineered for accurate measurement of human HO-1 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the HO-1 content in the sample. The color intensity is positively correlated with HO-1 content in the sample. The HO-1 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

HO-1, also called HMOX1, is the inducible isoform of heme oxygenase (HO) that is expressed in response to various stimuli to degrade heme leading to the generation of biliverdin, carbon monoxide, and release of ferrous iron. In essence, HO-1 exerts antioxidant, anti-apoptotic, and cytoprotective functions through its metabolic products as well as scavenging toxic intracellular heme. It also plays an important role in the prevention of vascular inflammation. HO-1 expression has been detected in a variety of cancers, including bladder, breast, and colorectal cancers.

Target Name

heme oxygenase (decycling) 1

Alternative Names

32 kD ELISA Kit; bK286B10 ELISA Kit; D8Wsu38e ELISA Kit; heat shock protein 32 kD ELISA Kit; heat shock protein 32kD ELISA Kit; Heat shock protein ELISA Kit; Heme oxygenase (decycling) 1 ELISA Kit; Heme oxygenase 1 ELISA Kit; Hemox ELISA Kit; HMOX 1 ELISA Kit; Hmox ELISA Kit; Hmox1 ELISA Kit; HMOX1_HUMAN ELISA Kit; HO 1 ELISA Kit; HO ELISA Kit; HO-1 ELISA Kit; HO1 ELISA Kit; Hsp32 ELISA Kit

Abbreviation

HMOX1

Uniprot No.

P09601

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 ng/mL-1000 ng/mL

Sensitivity

3.9 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-94
1:2	Average %	95
	Range %	89-99
1:4	Average %	98
	Range %	92-105
1:8	Average %	94
	Range %	88-99

Recovery

The recovery of human HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-92
EDTA plasma (n=4)	98	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.449	2.284	2.367	2.263
500	1.967	1.864	1.916	1.812
250	1.277	1.233	1.255	1.151
125	0.701	0.674	0.688	0.584
62.5	0.430	0.421	0.426	0.322
31.2	0.258	0.253	0.256	0.152
15.6	0.187	0.184	0.186	0.082
0	0.104	0.103	0.104

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Customer Reviews and Q&A

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■ Q&A

I want to measure serum levels of human heme oxygenase 1 (HO-1) in newborn. Your product (Uniprot No. P09601) is suitable for this purpose?
Its Code is CSB-E08266h.

Thanks for your inquiry.
We tested healthy human serum or plasma samples with CSB-E08266h before.It can be used to detect heme oxygenase 1 in newborn.But the results might be different.Pls follow your actual test results.

Target Background

Function (From Uniprot)	Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Gene References into Functions	Study found that plasma HO-1 levels were low in patients with peripheral artery disease, in contrast to high levels in patients with coronary artery disease. PMID: 30159103 HO-1 is over-expressed in sera of psoriasis patients and is correlated with psoriasis extent and severity. PMID: 30105448 Pregnant women who subsequently develop severe preeclampsia show higher expression of HO-1. PMID: 30363976 Our findings identify survivin as a target of HO-1 and a mediator of adipocyte-induced survival in the metastatic niche. PMID: 29311669 the role of HO-1 in nervous system pointing out different molecular mechanisms possibly responsible for HO-1 up-regulation in nervous system homeostasis and neurodegeneration. PMID: 30071692 HO-1 may serve as a prognostic marker and a new target to modulate chemotherapeutic effects in patients with small-intestinal adenocarcinomas PMID: 29537718 The T allele of heme oxygenase 1 gene SNP polymorphism (rs2071746) is a risk factor for esophageal varices development in cirrhotics. PMID: 29877949 Data show that the ferroptotic process induced by heme oxygenase-1 (HO-1). overexpression indicated that HO-1 is a key mediator of BAY 11-7085 (BAY)-induced ferroptosis that operates through cellular redox regulation and iron accumulation. PMID: 29274359 Heme oxygenase activity increases after exercise in healthy volunteers. PMID: 29343136 Results show that HO-1 is overexpressed in pancreatic cancer (PC) cells, induces cell proliferation and SHH signaling pathway activation as well as resistance to anticancer therapy. PMID: 29620188 the expression of hsa_circRNA0054633 has a protective effect against high glucoseinduced endothelial cell dysfunction by targeting ROBO1 and HO1. PMID: 29693114 the 14 kDa HO-1 is shown to promote cell proliferation and an increase in relative telomere lengths in vivo and in vitro. PMID: 29660345 HO-1 plays a key role in protecting tumor cells from apoptosis, in a process that involves Smad7 and HDAC4/5 in apoptosis of B-ALL cells PMID: 29886060 HO-1 might be a potential marker for prediction of ovarian cancer prognosis and a target for ovarian cancer treatment PMID: 28808929 HO-1 was an important cellular factor against Dengue virus replication. PMID: 27553177 our results demonstrate that Pc-induced expression of HO-1 is mediated by the PKCA-Nrf-2/HO-1 pathway, and inhibits UVB-induced apoptotic cell death in primary skin cells. PMID: 29470442 HO-1 regulates macrophage activation via the SIRT1-p53 signaling network and regulates hepatocellular death in liver ischemia-reperfusion injury. PMID: 28842295 In light of a pivotal role of NRF2 and BACH1 in response to oxidative stress and regulation of HO-1, we examined if smoke-induced HO-1 expression is modulated through the NRF2/BACH1 axis. We demonstrated that smoke causes significant nuclear translocation of NRF2, but only a slight decrease in nuclear BACH1. PMID: 29125538 downregulation of HO-1 gene expression in patients with inflammatory bowel disease. PMID: 28770550 This study demonstrated that HO-1 plays a vital role in the development of gastric cancer and may serve as a therapeutic target of this type of cancer PMID: 29048628 Overexpression of HO-1 inhibited the increase in nucleus pulposus cell apoptosis after IL-1beta treatment and simultaneously inhibited the expression of p-P65. PMID: 29617687 We also found a subset of prostate cancer patient-derived xenografts and prostate cancer patient samples with mild HO-1 and low Gal-1 expression levels. These results highlight a novel function of a human-used drug as a means of boosting the antitumor response PMID: 28512172 Our data suggest that HO-1 exerts its inhibitory effect on Th17 cell differentiation by directly associating and blocking STAT3 phosphorylation. We speculate that hemin may be a potential therapeutic candidate for the treatment of other types of immune and pulmonary inflammatory-related diseases. PMID: 28580587 recipient promoter region (GT)n genotype may contribute to cardiac allograft vasculopathy development PMID: 28186648 study implies that DJ-1 may protect endothelial progenitor cells against Ang II-induced dysfunction by activating the PPARgamma/HO-1. PMID: 28600848 These results revealed that hemin treatment increased HMOX1 expression and inhibited TGFbetainduced epithelial-mesenchymal transition in MCF7 cells. PMID: 28627599 There was no association between HMOX1 gene promoter polymorphism and manifestation of Fabry Disease. PMID: 28951772 Most published Newborn studies demonstrated no effect of HO-1 promoter polymorphisms on the Neonatal Hyperbilirubinemia. PMID: 28206992 Genetic mutations within MAPK1 (rs6928, rs9340, rs11913721), HIF-1 (rs1087314, rs2057482), and HO-1 (rs2071746) could alter susceptibility to perimenopausal coronary artery disease in Chinese population. PMID: 28444966 The overexpression of HO1 alleviated oxidative damage through inhibition of the PI3K/AKT signaling pathway and downregulation of the expression of cMyc. PMID: 28713890 Study shows that heme degradation by HO-1 is required for aggresome-like induced structures (ALIS) formation, and that the free iron released on heme degradation is necessary and sufficient to induce ALIS. PMID: 27821769 co-expression patterns of NGF and heme oxygenase-1 might be used as prognostic indicators for gastric carcinoma patients PMID: 28679437 These results suggest that HO-1 is involved in oxygen-glucose deprivation-evoked upregulation of apical junctional complex proteins, which is partly mediated by MMP9 pathway. PMID: 29425821 HMOX-1, an anti-oxidase, is a bona fide transcriptional target gene of HSF4 in HLECs (human lens epithelial cells). HSF4 directly binds to the HSE element in HMOX-1 promoter to mediate its mRNA transcription and protein accumulation. PMID: 29454088 The regulated HO-1 expression of Bone marrow stromal cells provides a new putative target for chronic myeloid leukemia therapy. PMID: 28445830 Dihydromyricetin (DMY) protects HUVECs from ox-LDL-induced oxidative injury by activating Akt and ERK1/2, which subsequently activates Nrf2/HO-1 signaling, thereby up-regulating antioxidant enzymes and anti-apoptotic proteins. PMID: 28612103 review of non-canonical functions of the HO isoforms: protein-protein interaction, intracellular compartmentalization, and extracellular secretion [review] PMID: 27626166 The interaction between ALAD rs1800435 and the HMOX1 rs2071746, HMOX1 rs2071747 was not associated with essential tremor. PMID: 28276576 Docosahexaenoic acid (DHA) activates Nrf2, possibly through modification of critical Keap1 cysteine 288 residue and PKCdelta-mediated phosphorylation of Nrf2, leading to upregulation of HO-1 and NQO1 expression. PMID: 28604588 These results suggest that RYR extract serves as alternative and complementary medicine in the treatment of these diseases, by inducing HO-1, thereby decreasing the vascular complications of diabetes PMID: 28555162 These results indicate that TNFRI-Fc and hHO-1 overexpression may apparently induce free iron in the liver and exert oxidative stress by enhancing reactive oxygen species production and block normal postneonatal liver metabolism. PMID: 28503569 Mice overexpressing human HO-1 specifically in astrocytes are protected from collagenase-induced intracerebral hemorrhage, as examined by striatal cell viability, neurological deficits and mortality. PMID: 28323022 HMOX1 negatively regulates MMP-9 expression in chronic lymphocytic leukemia cells in response to arsenic trioxide, through modulation of the p38 MAPK/AP-1 signaling pathway. PMID: 27829220 Review/Meta-analysis: both the HO-1(GT)n repeat length polymorphism and the T(-413)A SNP are associated with decreased risk of coronary heart disease. The (GT)n repeat length polymorphism was associated with coronary restenosis following PCI. PMID: 27825138 These data suggest that heme oxygenase-1 is induced during acute HIV-1 infection, likely mediating anti-inflammatory effects and driving expansion of heme oxygenase-1-specific CD8 regulatory T cells capable of suppressing HIV-1-specific immune responses in vitro. PMID: 28060008 Among myelodysplastic syndromes patients only, CD163 + macrophage density and HO1 and H-ferritin expression by CD163 + macrophages increased in tandem with marrow iron. High HO1 was significantly associated with shorter overall survival. PMID: 26758041 elevated HMOX1 expression is associated with stemness in Glioblastoma multiforme and can be modulated through TGFbeta. PMID: 27354342 Fetal GTn Repeat in the Heme Oxygenase 1 Promoter Is Associated With Severe and Early-Onset Preeclampsia. PMID: 29203625 CAL suppresses the expression of pro-inflammatory cytokines via p62/Nrf2-linked HO-1 induction in RASFs. PMID: 27678042 Findings indicate the importance of nuclear heme oxygenase-1 (HO-1) post-translational modification in the induction of cancer progression. PMID: 28846111 Show More Hide All
Involvement in disease	Heme oxygenase 1 deficiency (HMOX1D)
Subcellular Location	Microsome. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families	Heme oxygenase family
Tissue Specificity	Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).
Database Links	HGNC: 5013 OMIM: 141250 KEGG: hsa:3162 STRING: 9606.ENSP00000216117 UniGene: Hs.517581

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Check Code：

Code	CSB-E08266h
Size	96T,5×96T,10×96T
Trial Size
Have Questions?	Leave a Message or Start an on-line Chat

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.