Human heme oxygenase 1,HO-1 ELISA Kit

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Target Data

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Name: Human heme oxygenase 1,HO-1 ELISA Kit
Brand: CUSABIO
SKU: CSB-E08266h

Product Details

Target Name

heme oxygenase (decycling) 1

Alternative Names

HO-1, HSP32, bK286B10, heme oxygenase (decyclizing) 1

Abbreviation

HMOX1

Uniprot No.

P09601

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 ng/mL-1000 ng/mL

Sensitivity

3.9 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-94
1:2	Average %	95
	Range %	89-99
1:4	Average %	98
	Range %	92-105
1:8	Average %	94
	Range %	88-99

Recovery

The recovery of human HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-92
EDTA plasma (n=4)	98	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.449	2.284	2.367	2.263
500	1.967	1.864	1.916	1.812
250	1.277	1.233	1.255	1.151
125	0.701	0.674	0.688	0.584
62.5	0.430	0.421	0.426	0.322
31.2	0.258	0.253	0.256	0.152
15.6	0.187	0.184	0.186	0.082
0	0.104	0.103	0.104

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Still Have Questions?

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Q&A and Customer Reviews

■ Q&A

I want to measure serum levels of human heme oxygenase 1 (HO-1) in newborn. Your product (Uniprot No. P09601) is suitable for this purpose?
Its Code is CSB-E08266h.

Thanks for your inquiry.
We tested healthy human serum or plasma samples with CSB-E08266h before.It can be used to detect heme oxygenase 1 in newborn.But the results might be different.Pls follow your actual test results.

■ Customer Reviews

Target Data

Function	Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Involvement in disease	Heme oxygenase 1 deficiency (HMOX1D)
Subcellular Location	Microsome, Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side
Protein Families	Heme oxygenase family
Tissue Specificity	Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).
Database Links	HGNC: 5013 OMIM: 141250 KEGG: hsa:3162 STRING: 9606.ENSP00000216117 UniGene: Hs.517581

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Code	CSB-E08266h
Size	96T,5×96T,10×96T
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