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| Code | CSB-E08266h |
| Size | 96T,5×96T,10×96T |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-94 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-105 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 88-99 | |||||
| The recovery of human HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-92 | ||||
| EDTA plasma (n=4) | 98 | 90-105 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human HO-1 ELISA Kit is engineered for accurate measurement of human HO-1 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the HO-1 content in the sample. The color intensity is positively correlated with HO-1 content in the sample. The HO-1 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.
HO-1, also called HMOX1, is the inducible isoform of heme oxygenase (HO) that is expressed in response to various stimuli to degrade heme leading to the generation of biliverdin, carbon monoxide, and release of ferrous iron. In essence, HO-1 exerts antioxidant, anti-apoptotic, and cytoprotective functions through its metabolic products as well as scavenging toxic intracellular heme. It also plays an important role in the prevention of vascular inflammation. HO-1 expression has been detected in a variety of cancers, including bladder, breast, and colorectal cancers.
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I want to measure serum levels of human heme oxygenase 1 (HO-1) in newborn. Your product (Uniprot No. P09601) is suitable for this purpose?
Its Code is CSB-E08266h.
