Human indoleamine 2,3-dioxygenase,IDO ELISA Kit

Code CSB-E09966h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P14902
Lead Time 3-5 working days
Abbreviation IDO1
Target Name indoleamine 2,3-dioxygenase 1
Alias CD107B, IDO, INDO, indole 2,3-dioxygenase|indoleamine-pyrrole 2,3 dioxygenase
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IDO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %85-99
1:2Average %95
Range %91-102
1:4Average %89
Range %84-94
1:8Average %93
Range %89-98
Recovery
The recovery of human IDO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9791-104
EDTA plasma (n=4)8680-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.574 2.552 2.563 2.461
252.078 1.949 2.014 1.912
12.51.228 1.278 1.253 1.151
6.250.537 0.560 0.549 0.447
3.120.326 0.340 0.333 0.231
1.560.204 0.197 0.201 0.099
0.780.159 0.152 0.156 0.054
00.103 0.100 0.102  
References
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Function Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway
Subcellular Location Cytoplasm, cytosol
Protein Families Indoleamine 2,3-dioxygenase family
Tissue Specificity Expressed in mature dendritic cells located in lymphoid organs (including lymph nodes, spleen, tonsils, Peyers's patches, the gut lamina propria, and the thymic medulla), in some epithelial cells of the female genital tract, as well as in endothelial cell
Database Links

HGNC: 6059

OMIM: 147435

KEGG: hsa:3620

STRING: 9606.ENSP00000430505

UniGene: Hs.840

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