IDO1 Antibody

Datasheet
Code CSB-PA010996HA01HU
See More Details Free Antibody trial simple
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
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  • Immunohistochemistry analysis of human cervical cancer using CSB-PA010996HA01HU at dilution of 1:100

  • Immunofluorescent analysis of HepG2 cells using CSB-PA010996HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Western Blot
    Positive WB detected in: U87 whole cell lysate
    All lanes: IDO1 antibody at 2.6µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • Immunofluorescence staining of Hela cells with CSB-PA010996HA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) IDO1 Polyclonal antibody
Uniprot No. P14902
Target Names IDO1
Alternative Names Indoleamine 2,3-dioxygenase 1 (IDO-1) (EC 1.13.11.52) (Indoleamine-pyrrole 2,3-dioxygenase), IDO1, IDO INDO
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Indoleamine 2,3-dioxygenase 1 protein (1-403AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Citations

O-cyclic phytosphingosine-1-phosphate stimulates HIF1α-dependent glycolytic reprogramming to enhance the therapeutic potential of mesenchymal stem cells. Lee HJ, et al,Cell Death and Disease,2019

Applications: WB
Review: The protein expressions of VEGF, EGF, IL-6, IDO-1, and β-Actin in tissue samples were measured by western blotting. n = 4–5. All blot and immunofluorescence images are representative. Quantitative data are presented as a mean ± S.E.M with scatter plots. *indicates p < 0.05. N.D. indicates not detected.
PMID: 31383843

Target Data

Function Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway
Subcellular Location Cytoplasm, cytosol
Protein Families Indoleamine 2,3-dioxygenase family
Tissue Specificity Expressed in mature dendritic cells located in lymphoid organs (including lymph nodes, spleen, tonsils, Peyers's patches, the gut lamina propria, and the thymic medulla), in some epithelial cells of the female genital tract, as well as in endothelial cell
Database Links

HGNC: 6059

OMIM: 147435

KEGG: hsa:3620

STRING: 9606.ENSP00000430505

UniGene: Hs.840

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