Code | CSB-RA215054A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The IDO1 recombinant monoclonal antibody is designed to detect human IDO1 protein and can be utilized in ELISA and IHC applications. The production of this antibody involves recombinant DNA technology and begins with the sequencing of the cDNA from the IDO1 antibody-producing hybridomas. The IDO1 monoclonal antibody gene is then synthesized and cloned into a vector for transfection into cells for cultivation. The hybridomas are created by fusing B cells that are extracted from an animal immunized with a synthesized peptide derived from human IDO1 with myeloma cells. Finally, the IDO1 recombinant monoclonal antibody is purified through affinity chromatography from the cell culture supernatant.
The IDO1 protein is an enzyme that catalyzes the initial and rate-limiting step of tryptophan metabolism along the kynurenine pathway. In cells, IDO1 is primarily expressed in immune cells, such as dendritic cells, macrophages, and some tumor cells. Its main function is to regulate immune responses, specifically by limiting T-cell activation and promoting regulatory T-cell differentiation. The production of kynurenine from tryptophan by IDO1 has also been implicated in other cellular processes such as neuroprotection, immunotolerance, and inflammation. Dysfunction of IDO1 has been associated with various diseases, including cancer, autoimmunity, and neurodegeneration.
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