Human keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit

Code CSB-E04496h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P15514
Lead Time 3-5 working days
Abbreviation AREG
Target Name amphiregulin
Alias AR, CRDGF, MGC13647, SDGF, colorectum cell-derived growth factor|schwannoma-derived growth factor
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 4.7 pg/mL-300 pg/mL
Sensitivity 1.1 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. This encoded protein is associated with a psoriasis-like skin phenotype.
HGNC 651
RGD 2149
MGI 88068
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 86-97  
1:2 Average % 83  
Range % 80-86  
1:4 Average % 91  
Range % 85-97  
1:8 Average % 98  
Range % 95-101  
Recovery
The recovery of human KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-93  
EDTA plasma (n=4) 104 100-108  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
300 2.812 2.836 2.824 2.681  
150 1.971 1.965 1.968 1.825  
75 1.122 1.168 1.145 1.002  
37.5 0.590 0.571 0.581 0.438  
18.8 0.385 0.399 0.392 0.249  
9.4 0.293 0.273 0.283 0.140  
4.7 0.210 0.216 0.213 0.070  
0 0.142 0.144 0.143    
Still Have Questions? Leave a Message or Start an on-line Chat
Function Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
Subcellular Location Membrane, Single-pass membrane protein
Protein Families Amphiregulin family
Database Links

HGNC: 651

OMIM: 104640

KEGG: hsa:374

STRING: 9606.ENSP00000370227

UniGene: Hs.270833

Pathway ErbB signaling pathway
Hippo signaling pathway
MAPK signaling pathway
PI3K-Akt signaling pathway

Related Products

AREG Antibodies

AREG Antibodies for Homo sapiens (Human)

AREG Antibodies for Human

AREG Proteins

AREG Proteins for Homo sapiens (Human)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.