Mouse L-lactate dehydrogenase A chain(LDH-A)ELISA Kit

Instructions
Code CSB-E17733m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lactate dehydrogenase A
Alternative Names Ldha ELISA Kit; Ldh-1 ELISA Kit; Ldh1L-lactate dehydrogenase A chain ELISA Kit; LDH-A ELISA Kit; EC 1.1.1.27 ELISA Kit; LDH muscle subunit ELISA Kit; LDH-M ELISA Kit
Abbreviation LDHA
Uniprot No. P06151
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 mU/mL-10 mU/mL
Sensitivity 0.039 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDH-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 100
Range % 95-106
1:200 Average % 88
Range % 84-93
1:400 Average % 107
Range % 103-110
1:800 Average % 86
Range % 83-90
Recovery
The recovery of mouse LDH-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 96-108
EDTA plasma (n=4) 85 81-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average
10 0.144 0.148 0.146
5 0.215 0.211 0.213
2.5 0.325 0.331 0.328
1.25 0.508 0.499 0.504
0.625 0.763 0.778 0.771
0.312 1.230 1.241 1.236
0.156 1.561 1.570 1.566
0 2.180 2.034 2.107
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Gene References into Functions
  1. The release of lactate dehydrogenase A (LDHA) from degenerating neurons drives central nervous system (CNS) angiogenesis. PMID: 29248508
  2. Hair follicle stem cells maintain a metabolic state that allows them to remain dormant and yet quickly respond to appropriate proliferative stimuli mediated by LDH-A signaling. PMID: 28812580
  3. Results revealed that beta-arrestin-1 regulates lactate metabolism to contribute to beta2-adrenergic receptor functions in improved memory formation. PMID: 27916196
  4. The authors identified lactate dehydrogenase (LDH) as a new functional target of AMPKalpha1. PMID: 28515121
  5. LDHA-associated lactic acid accumulation in melanomas inhibits tumor surveillance by T and natural killer cells. PMID: 27641098
  6. lactate dehydrogenase A (LDHA) is induced in activated T cells to support aerobic glycolysis but promotes IFN-gamma expression independently of its 3'UTR. PMID: 27708054
  7. LDHA is a direct target of miR-34a in regulating glucose metabolism and tumor growth in breast cancer. PMID: 26902416
  8. Findings suggest that RANKL protein-induced lactate dehydrogenase (LDH) activation stimulates glycolytic and mitochondrial respiratory metabolism via transcription factor NFATc1 signaling. PMID: 27077737
  9. We propose a profibrotic feed forward loop, in which radiation induces LDHA expression and lactate production, which can lead to further activation of TGF-beta to drive the fibrotic process. PMID: 26254422
  10. High LDH-A expression is associated with metastases in breast tumors. PMID: 23833310
  11. PGC-1alpha reduces the expression of LDH A and one of its regulators, the transcription factor myelocytomatosis oncogene. PMID: 23650363
  12. Lactate dehydrogenase A is overexpressed in pancreatic cancer and promotes the growth of pancreatic cancer. PMID: 23404405
  13. Down-regulation of expression of stress-activated genes PKC-alpha, c-Myc and LDH-A by alpha-tocopherol in cancerous mice. PMID: 22132835
  14. The cumulus cells and oocytes showed intense signals for lactate dehydrogenase (LDH)-A respectively. PMID: 21360229
  15. isoproterenol induces changes in gastrocnemius muscle and serum lactate dehydrogenase expression in mice PMID: 21552790
  16. Cocaine could inhibit the activities of ATPase, LDH and SDH in cultured splenocytes. PMID: 20653129
  17. The increased LDH-A/LDH-B ratio causes high brain lactate levels, which, in turn, are predictive of aging phenotypes. PMID: 21041631
  18. The muscle subunit of lactate dehydrogenase is an integral part of the sarcolemmal K(ATP) channel complex in vivo, where it couples the metabolic status of the cell with the K(ATP) channel activity essential for protection against ischemia. PMID: 12145195
  19. Reduction in LDH-A activity resulted in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. The tumorigenicity of the LDH-A-deficient cells was severely diminished. PMID: 16766262
  20. beta-agonists increased serum LDH levels, but it was not due to release from the heart. PMID: 17853776
  21. M-LDH released from cardiomyocytes after hypoxia and reoxygenation has a role in protecting the heart from oxidative stress-induced injury through an intracellular signal transduction pathway involving ERK1/2. PMID: 18534967

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Subcellular Location Cytoplasm
Protein Families LDH/MDH superfamily, LDH family
Database Links

KEGG: mmu:16828

STRING: 10090.ENSMUSP00000103267

UniGene: Mm.29324

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