Recombinant Mouse L-lactate dehydrogenase A chain (Ldha)

Code CSB-YP012832MO
MSDS
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Source Yeast
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Code CSB-EP012832MO
MSDS
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Source E.coli
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Code CSB-EP012832MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP012832MO
MSDS
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Source Baculovirus
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Code CSB-MP012832MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ldha
Uniprot No.
Alternative Names
Ldha; Ldh-1; Ldh1L-lactate dehydrogenase A chain; LDH-A; EC 1.1.1.27; LDH muscle subunit; LDH-M
Species
Mus musculus (Mouse)
Expression Region
2-332
Target Protein Sequence
ATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVMEDKLKG EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Gene References into Functions
  1. The release of lactate dehydrogenase A (LDHA) from degenerating neurons drives central nervous system (CNS) angiogenesis. PMID: 29248508
  2. Hair follicle stem cells maintain a metabolic state that allows them to remain dormant and yet quickly respond to appropriate proliferative stimuli mediated by LDH-A signaling. PMID: 28812580
  3. Results revealed that beta-arrestin-1 regulates lactate metabolism to contribute to beta2-adrenergic receptor functions in improved memory formation. PMID: 27916196
  4. The authors identified lactate dehydrogenase (LDH) as a new functional target of AMPKalpha1. PMID: 28515121
  5. LDHA-associated lactic acid accumulation in melanomas inhibits tumor surveillance by T and natural killer cells. PMID: 27641098
  6. lactate dehydrogenase A (LDHA) is induced in activated T cells to support aerobic glycolysis but promotes IFN-gamma expression independently of its 3'UTR. PMID: 27708054
  7. LDHA is a direct target of miR-34a in regulating glucose metabolism and tumor growth in breast cancer. PMID: 26902416
  8. Findings suggest that RANKL protein-induced lactate dehydrogenase (LDH) activation stimulates glycolytic and mitochondrial respiratory metabolism via transcription factor NFATc1 signaling. PMID: 27077737
  9. We propose a profibrotic feed forward loop, in which radiation induces LDHA expression and lactate production, which can lead to further activation of TGF-beta to drive the fibrotic process. PMID: 26254422
  10. High LDH-A expression is associated with metastases in breast tumors. PMID: 23833310
  11. PGC-1alpha reduces the expression of LDH A and one of its regulators, the transcription factor myelocytomatosis oncogene. PMID: 23650363
  12. Lactate dehydrogenase A is overexpressed in pancreatic cancer and promotes the growth of pancreatic cancer. PMID: 23404405
  13. Down-regulation of expression of stress-activated genes PKC-alpha, c-Myc and LDH-A by alpha-tocopherol in cancerous mice. PMID: 22132835
  14. The cumulus cells and oocytes showed intense signals for lactate dehydrogenase (LDH)-A respectively. PMID: 21360229
  15. isoproterenol induces changes in gastrocnemius muscle and serum lactate dehydrogenase expression in mice PMID: 21552790
  16. Cocaine could inhibit the activities of ATPase, LDH and SDH in cultured splenocytes. PMID: 20653129
  17. The increased LDH-A/LDH-B ratio causes high brain lactate levels, which, in turn, are predictive of aging phenotypes. PMID: 21041631
  18. The muscle subunit of lactate dehydrogenase is an integral part of the sarcolemmal K(ATP) channel complex in vivo, where it couples the metabolic status of the cell with the K(ATP) channel activity essential for protection against ischemia. PMID: 12145195
  19. Reduction in LDH-A activity resulted in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. The tumorigenicity of the LDH-A-deficient cells was severely diminished. PMID: 16766262
  20. beta-agonists increased serum LDH levels, but it was not due to release from the heart. PMID: 17853776
  21. M-LDH released from cardiomyocytes after hypoxia and reoxygenation has a role in protecting the heart from oxidative stress-induced injury through an intracellular signal transduction pathway involving ERK1/2. PMID: 18534967

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Subcellular Location
Cytoplasm.
Protein Families
LDH/MDH superfamily, LDH family
Database Links
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