Mouse interleukin 12,IL-12/P40 ELISA KIT

Instructions
Code CSB-E07360m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Alternative Names Il12bInterleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit
Abbreviation IL12B
Uniprot No. P43432
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:5Average %92
Range %88-95
1:10Average %90
Range %85-92
1:20Average %89
Range %84-96
1:40Average %92
Range %86-98
Recovery
The recovery of mouse IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-100
EDTA plasma (n=4)9387-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.701 2.852 2.777 2.633
5002.005 2.076 2.041 1.897
2501.248 1.316 1.282 1.138
1250.754 0.704 0.729 0.585
62.50.476 0.450 0.463 0.319
31.20.311 0.323 0.317 0.173
15.60.252 0.259 0.256 0.112
00.148 0.139 0.144  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Gene References into Functions
  1. In macrophages, SCIMP is constitutively associated with the Lyn tyrosine kinase, is required for tyrosine phosphorylation of TLR4, and facilitates TLR-inducible production of the IL-6 and IL-12p40. PMID: 28098138
  2. Results show the overproduction of IL-12/23p40 in dendritic cells, leading to Th1 and Th17 programs in a mouse model of Yersinia enterocolitica-induced reactive arthritis. PMID: 29494692
  3. this study demonstrates that regulation of IL-12p40 by hypoxia-inducible factor-1alpha controls Th1/Th17 responses to prevent mucosal inflammation PMID: 28120851
  4. the nuclear localized CA-VI B selectively promotes IL-12 expression by interaction with protein arginine N-methyltransferase 5 (PRMT5), which reduces symmetric dimethylation of histone H3 arginine 8 modification (H3R8me2s) at Il12 promoters to facilitate chromatin accessibility, selectively enhancing c-Rel binding to the Il12b promoter. PMID: 28739930
  5. High dilutions of antimony modulate cytokines production and macrophage - Leishmania (L.) amazonensis interaction in vitro.( PMID: 28092793
  6. IL-12p40 depletion in mice could promote ectopic bone formation. PMID: 27472064
  7. Data indicate that there was a differential transcription of 381 genes which changed greater than two fold in B1a of p40-/-IL-2Ralpha-/- mice. PMID: 27105495
  8. this study shows that endosomal signalling by Lactobacillus acidophilus that leads to IL-12 production is inhibited by TLR stimulation from the plasma membrane PMID: 27441725
  9. Furthermore, IL-12p40-/- mice showed decreased sensitization to inhaled cockroach antigen after PVM infection when compared to wild-type mice. PMID: 27400340
  10. The study demonstrate a protective role of IL-12Rbeta2 in relapsing-remitting experimental autoimmune encephalomyelitis. PMID: 26857496
  11. Interleukin-12/23p40 plays an essential role in the development of graft-versus-host disease in mice. PMID: 25846718
  12. Data indicate that sera from treated with cholesterol-p40 (Il12b)-siRNA contained significantly reduced concentrations of p40. PMID: 26232431
  13. this study demonstrates viral RNA analog-induced transcription synergy of Il6 and Il12b via IFN regulatory factor (IRF)1 (TLR3-TIR domain-containing adaptor inducing IFN-beta [TRIF] responsive), C/EBPbeta (TLR7-MyD88 responsive), and JunB (all responsive). PMID: 26109639
  14. our results suggest that IL-12p40 acts as a negative regulator of autoimmune cholangitis in IL-2Ralpha-/- mice. Compared to IL-2Ralpha-/- mice, p40-/-IL-2Ralpha-/- mice develop liver fibrosis and reduced colitis. PMID: 24651036
  15. These results imply that a soluble host or Toxoplasma gondii factor is responsible for the bulk of IL-12p40 production in vivo. PMID: 25024368
  16. the assembled p40-CD5L heterodimer does not recapitulate the biological activity of IL-12. PMID: 24821971
  17. The immune-enhancing effect of the Cronobacter sakazakii ES2 phage results in the activation of nuclear factor-kappaB and dendritic cell maturation via the activation of IL-12p40 in the mouse bone marrow. PMID: 24184907
  18. IL-12 does not induce the expression of IL-7 in microglia and macrophages via both IL-12Rbeta2 and IL-12Rbeta1. PMID: 24224652
  19. Data indicate that the GCN2 knockout mice showed reduced inflammatory responses, with decreased IL-6 and IL-12 expression correlating with significant reduction in animal mortality. PMID: 24248597
  20. IL-12p40 contributed most in inducing Th1 and Th17 polarization PMID: 23697689
  21. IL-12p40 deficiency leads to uncontrolled Trypanosoma cruzi dissemination in the spinal cord resulting in neuronal death and motor dysfunction. PMID: 23152844
  22. Klf10 is a transcription factor that regulates the expression of IL-12p40 in bone marrow-derived macrophages. PMID: 23065757
  23. IL-18 and IL-12 exert a synergistic effect on the lungs by inducing MMPs, cathepsins S, and pro-inflammatory cytokines, which may promote pulmonary emphysema and inflammation. PMID: 22908875
  24. Inhibition of IL-12/IL-23 signaling reduces Alzheimer's disease-like pathology and cognitive decline. PMID: 23178247
  25. Novel IL27p28/IL12p40 cytokine suppressed experimental autoimmune uveitis by inhibiting autoreactive Th1/Th17 cells and promoting expansion of regulatory T cells. PMID: 22936807
  26. TLR2 plays a dual role in regulating TLR9-dependent IL-12p40 production in dendritic cells. PMID: 22635254
  27. in mice deficient in IL-12p40, human metapneumovirus infection induced an exacerbated pulmonary inflammatory response and mucus production, altered cytokine response, and decreased lung function PMID: 22606349
  28. reactive oxygen species are important for IL-12 production induced by lactobacillus PMID: 22536449
  29. MKP-1 increases the expression of IL-12 by enhancing the expression of IRF1 PMID: 22464096
  30. extracellular signal regulated kinases modulate IL-12 expression by regulating IkappaB-zeta-dependent binding of NF-kappaB transcription factors to IL-12b gene promoter PMID: 22131231
  31. Francisella tularensis LVS induced innate production of IL-12p(40)2 is required for dendritic cell migration. PMID: 21669537
  32. microbiota negatively regulates host miR-10a expression, which may contribute to the maintenance of intestinal homeostasis by targeting IL-12/IL-23p40 expression. PMID: 22068236
  33. Data show that the p40 vaccine induced specific antibodies to IL-12 and IL-23, which was associated with improvement of intestinal inflammation and fibrosis. PMID: 21424108
  34. dendrittic cells-derived, controls acute infection by Toxoplasma gondii PMID: 21867928
  35. MW and glucocorticoids independently modulate IL-12p40 production through a mechanism involving ERK1/2 hyperactivation and that glucocorticoids can significantly augment MW-induced inhibition of IL-12p40. PMID: 21730055
  36. A distal enhancer in Il12b is the target of transcriptional repression by the STAT3 pathway and requires the basic leucine zipper (B-ZIP) protein NFIL3. PMID: 21566115
  37. Data identified NFIL3 as a regulatory transcription factor in macrophages, controlling IL-12 p40 production. PMID: 21383239
  38. The colitis-prone SJL/J-derived polymorphic variant of IL12b p40 more readily forms p70 heterodimers than does the colitis-resistant C57BL/6 strain-derived p40 variant. PMID: 21321105
  39. PKCdelta is a regulator of IL-12p40/p70 production by dendritic cells and macrophages, driving the healer phenotype during cutaneous leishmaniasis. PMID: 21287553
  40. macrophage-derived Arg I protects hosts against excessive tissue injury caused by worm eggs during acute schistosomiasis by suppressing IL-12/IL-23p40 PMID: 20483789
  41. CD14(neg) B6 mice showed delayed production of IL12p40 in response to necrotic cells PMID: 19840871
  42. These studies demonstrated that IL-12 plays a predominant role in the expansion of effector CD8(+) T-cell immunity against Encephalitozoon cuniculi, which is critical for host survival. PMID: 20308292
  43. there is a critical role for the tyrosine phosphatase activity of SHP-1 for induction of IL-12p40 production in macrophages in response to TLR ligands PMID: 20145200
  44. Results show that IL-12-->IL-27 sequential gene therapy provides a simple but effective approach for eliminating inaccessible residual tumors. PMID: 20139275
  45. IL-12p40 induction in macrophages is directly regulated by the intracellular glutathione-redox status of mouse macrophages in vitro. PMID: 20164428
  46. We suggest that overproduction of NO may account for neuronal death at the spinal cord of T. cruzi-infected IL-12p40KO mice and that IFN-gamma and S100beta may contribute to NOS activation in the absence of IL-12. PMID: 19923851
  47. We found that the production of IL-12 preceded that of other cytokines in response to Influenza A virus lung infection. PMID: 19941386
  48. Langerin-positive cells are not required for initial activation of invariant natural killer T (iNKT) cells, but are required for downstream release of large quantities of IL-12p70 into the serum. PMID: 19923446
  49. protective and agonistic function in mycobacterial infection PMID: 11739515
  50. Heat shock inhibits IL-12 p40 expression through NF-kappa B signalling pathway in murine macrophages PMID: 11792125

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Subcellular Location Secreted
Protein Families Type I cytokine receptor family, Type 3 subfamily
Database Links

KEGG: mmu:16160

STRING: 10090.ENSMUSP00000099860

UniGene: Mm.239707

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