Mouse transforming growth factorβ2,TGFβ2 ELISA Kit

Code CSB-E09785m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse TGFB2 ELISA Kit was designed for the quantitative measurement of Mouse TGFB2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .

Target Name transforming growth factor, beta 2
Alternative Names Tgfb2 ELISA Kit; Transforming growth factor beta-2 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-2 ELISA Kit; TGF-beta-2)] ELISA Kit
Abbreviation TGFB2
Uniprot No. P27090
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGFβ2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %97
Range %92-102
1:200Average %91
Range %85-98
1:400Average %90
Range %88-93
1:800Average %100
Range %96-104
Recovery
The recovery of mouse TGFβ2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8987-92
EDTA plasma (n=4)9489-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
1002.432 2.552 2.492 2.352
502.041 2.076 2.059 1.919
251.546 1.604 1.575 1.435
12.51.002 1.009 1.006 0.866
6.250.509 0.524 0.517 0.377
3.120.334 0.359 0.347 0.207
1.560.238 0.229 0.234 0.094
00.135 0.145 0.140  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.; Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2.; Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.
Gene References into Functions
  1. data suggest a mechanism whereby a stromal hedgehog-TGFbeta2 signaling axis acts to control nephrogenesis. PMID: 29945868
  2. TGF-beta2 as the crucial mediator of neural precursor cell immunomodulation. PMID: 28945200
  3. CREBH was identified as a key positive regulator of TGF-beta2 transcription in hepatitis C virus-infected cells. PMID: 28621467
  4. RUNX1T1 serves as a common angiogenic driver for vaculogenesis and functionality of endothelial lineage cells PMID: 28640846
  5. APC-derived TSP-1 is essential for the development of an adaptive regulatory immune response induced by TGF-beta2-expressing APCs similar to those located at mucosal and ocular sites. PMID: 26458493
  6. Epidermal Tgfb2 controls proliferation, differentiation and ECM production by reticular fibroblasts. PMID: 26837596
  7. Thus, along with TGF-b and MAPK signaling, NFkappaB serves as an important regulatory pathway which following Losartan treatment PMID: 25766329
  8. Expression of TGFbeta2 was induced in response to elevated canonical Wnt signaling in dystrophic muscles and that the resulting increase in TGFbeta activity affected the behavior of satellite cells in an autocrine or paracrine fashion. PMID: 25520397
  9. these data shed light on previously unrecognized roles of Mkx in tendinopathy, tenogenesis, and tendon repair as well as in regulating the TGFbeta pathway. PMID: 25332192
  10. TGFbeta2 regulates hypothalamic TRH expression through TIEG1 during fetal development. PMID: 25448845
  11. These findings indicate that the lens epithelium of MCT mice has increased expression of TGFb1 and Tgfb2 before cataract affection and that changes in the expression of FGF2 as well as TGFbeta may contribute to the progression of the cataract in the mice. PMID: 24279395
  12. Endothelial lineage differentiation from induced pluripotent stem cells is regulated by microRNA-21 and transforming growth factor beta2 (TGF-beta2) pathways PMID: 24356956
  13. work reveals a 'seed and soil' mechanism where TGF-beta2 and TGF-beta-RIII signalling through p38alpha/beta regulates DTC dormancy and defines restrictive (BM) and permissive (lung) microenvironments for HNSCC metastasis PMID: 24161934
  14. HD3alpha directly interacts with HDAC3 and Akt1 and selectively activates transforming growth factor beta2 (TGFbeta2) secretion and cleavage. PMID: 24045946
  15. In fetal testis, both cord maintenance and somatic cell development are highly sensitive to the levels of TGFbeta2. PMID: 23303681
  16. the microenvironment at the verge between inflammation (IL-1beta) and tissue remodeling (TGFbeta2) can strongly promote the process of EndMT. PMID: 22739237
  17. MKL2 regulates a conserved TGF-beta signaling pathway that is required for angiogenesis and ultimately embryonic survival. PMID: 22899851
  18. haploinsufficient Tgfb2(+/-) mice have aortic root aneurysm and biochemical evidence of increased canonical and noncanonical TGF-beta signaling PMID: 22772368
  19. Data indicate that TGFbeta2 promotes valve remodeling and differentiation by inducing matrix organization and suppressing cushion mesenchyme differentiation into cartilage cell lineage during heart development. PMID: 21780244
  20. These observations identify a novel regulatory axis that includes PPARs, Prdm16, and TGF-beta2 in hematopoiesis. PMID: 21967974
  21. Laser capture microdissection collected clean populations of primary lip and palate epithelial cells destined to fuse. Transcripts of Bmp-4/Alk-3 and Tgfbeta-2/Alk-5 were restricted to epithelial seam of the fusion site, and epithelium of prefusion site. PMID: 21959449
  22. beta-catenin causes renal dysplasia via upregulation of Tgfbeta2 and Dkk1. PMID: 21436291
  23. High TGFbeta 2 is associated with SMO-mediated basal cell carcinoma development. PMID: 20858897
  24. autocrine stimulation of myeloid progenitors by Tgfbeta2 as one mechanism by which HoxA10 expands this population. PMID: 21087928
  25. TGFbeta2 activation of MEKK3/ERK1/2/5 signaling modulates Has2 expression and hyaluronan production leading to the induction of epithelial to mesenchymal transformation events. PMID: 20633555
  26. TGF-beta2 suppressed macrophage cytokine production in the developing intestine and protected against inflammatory mucosal injury. PMID: 20875417
  27. Gene expression analysis shows that transcripts of tgfbeta2 and tgfbeta3 but not tgfbeta1 are abundant in the growing undifferentiated limb mesoderm. PMID: 20565961
  28. Reduction of TGFbeta2 gene dosage in the RA receptor-deficient background restores septation but does not rescue alignment defects, indicating that excess TGFbeta causes septation defects. PMID: 20230754
  29. Data show that TNF-alpha induces the formation of fibrotic foci by cultured retinal pigment epithelial cells through activation of transforming growth factor-TGF-beta signaling in a manner dependent on hyaluronan-CD44-moesin interaction. PMID: 19965872
  30. the perichondrium is essential for the effects of Shh on chondrocyte differentiation but that TGFbeta2 specifically is required for this effect. TGFbeta2 is required for Shh-mediated of PTHrP expression. PMID: 11934857
  31. TGFbeta2 plays a role in apoptosis in interdigital webs of the developing mouse limb PMID: 11960699
  32. TGFbeta2 is expressed and obligatory for mammalian endocardial cushion cell transformation PMID: 12142029
  33. Altered renin synthesis and secretion in the kidneys of heterozygous mice with a null mutation in the TGF-beta(2) gene. Renal renin mRNA and protein levels were higher in mutant mice under hydrated or dehydrated conditions. PMID: 12381922
  34. results show that TGF-betas obviously play a fundamental role in midline fusion and the TGFbeta2 gene seems to play a more important role in mediating developmental processes than the TGFbeta3 gene PMID: 12478370
  35. TGFb2 is regulated by the type III transforming growth factor beta receptor PMID: 12773577
  36. Tgfbeta2 has roles in myocardialisation and in epicardial development PMID: 12815630
  37. TGF-beta2 plays essential roles in cornea and lens development PMID: 12838410
  38. TGF-beta2 is a genetically determined positive regulator of primitive hematopoietic progenitor cells PMID: 14707111
  39. The TGF-beta2 suggested to play a role in the regulation of chromaffin cells proliferation and differentiation based on in vitro experiments. PMID: 15478122
  40. A high degree of TGF-ss2-specific SMAD2 signaling goes concomitant with vascular innervation of the fourth arch artery. PMID: 15528466
  41. TGF beta 2 contributes to the development of the cochleovestibular ganglion in mouse embryos PMID: 15744664
  42. HDL subfraction 3 selectively induces TGF-beta2 mRNA expression and protein release PMID: 15911702
  43. The highly restrictive and spatial and temporal pattern of expression of Tgf-beta2 implicates its role in mediating the fusion of developing midfacial processes, possibly through interacting with bone morphogenetic protein 4 (Bmp4) PMID: 16026545
  44. The results clearly demonstrate that TGF-beta2 and TGF-beta3 are essential signals for differentiation of midbrain progenitors toward neuronal fate and dopaminergic phenotype. PMID: 16741229
  45. Pax3 regulation of TGFbeta2 may play a pivotal role during early embryonic development PMID: 16787918
  46. We conclude that striatal TGF-beta may be essential for permitting exogenous GDNF to act as a neuroprotective factor. PMID: 17141511
  47. Zac1 employs a novel cell non-autonomous strategy to regulate amacrine cell number, acting in cooperation with a second tumor suppressor gene, TGFbetaII, through a negative feedback pathway. PMID: 17559664
  48. TGFbeta2 pathway is critical in regulating morphogenesis in response to noncanonical Wnt signaling. PMID: 17767158
  49. Findings suggest that augmented nephron number in TGF-beta2(+/-) kidneys results from accelerated ureteric branching morphogenesis and nephron formation. PMID: 18317401
  50. Data show that TGF-beta2 influences synaptic function, rather than synaptogenesis, specifically at central synapses. PMID: 18854036

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Subcellular Location [Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-2]: Secreted.
Protein Families TGF-beta family
Database Links

KEGG: mmu:21808

STRING: 10090.ENSMUSP00000043849

UniGene: Mm.18213

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