Rat Endothelial nitric oxide synthase,eNOS/NOS3 ELISA Kit

Instructions
Code CSB-E08323r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name nitric oxide synthase 3 (endothelial cell)
Alternative Names Nos3 ELISA Kit; Nitric oxide synthase ELISA Kit; endothelial ELISA Kit; EC 1.14.13.39 ELISA Kit; Constitutive NOS ELISA Kit; cNOS ELISA Kit; EC-NOS ELISA Kit; Endothelial NOS ELISA Kit; eNOS ELISA Kit; NOS type III ELISA Kit; NOSIII ELISA Kit
Abbreviation NOS3
Uniprot No. Q62600
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, cell lysates
Detection Range 15.6 μIU/mL-1000 μIU/mL
Sensitivity 3.9 μIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat eNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %96
Range %92-100
1:200Average %92
Range %90-95
1:400Average %91
Range %85-96
1:800Average %89
Range %85-98
Recovery
The recovery of rat eNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9285-97
EDTA plasma (n=4)9492-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/ml. OD1OD2AverageCorrected
10002.203 2.304 2.254 2.077
5001.914 1.934 1.924 1.747
2501.477 1.498 1.488 1.311
1250.953 0.951 0.952 0.775
62.50.534 0.571 0.553 0.376
31.20.391 0.399 0.395 0.218
15.60.317 0.325 0.321 0.144
00.175 0.178 0.177  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

Can I test this kit with skin tissue?

A:
Thanks for your inquiry. Sorry we did not ever use this kit to test skin tissue samples before.
We suggest you do a pretest first if you want to have a try.

Target Data

Function Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Gene References into Functions
  1. Low NOS3 expression is associated with diabetic endothelial dysfunction. PMID: 29471582
  2. AM and L-NAME eliminated the CAPE-oNO2-mediated cardioprotection by restraining SIRT1 and eNOS expression, respectively. It suggested that CAPE-oNO2 ameliorated MIRI by suppressing the oxidative stress, inflammatory response, fibrosis and necrocytosis via the SIRT1/eNOS/NF-kappaB pathway PMID: 29220696
  3. The present results indicate that Advanced glycation endproducts impairs endothelial-dependent vasodilation, and this effect is mediated via arginase overexpression and NADPH oxidase stimulation. PMID: 29627571
  4. Activation of ACE2 had a protective role in the development of pulmonary arterial hypertension in rat model via improving the function of pulmonary arterial endothelium. This effect was potentially mediated by promoted NO release as a consequence of increased phosphorylation of eNOS at Ser1177 and dephosphorylation of eNOS at Thr495. PMID: 29146157
  5. current studies demonstrate that PYK2 is a pivotal regulator of eNOS function in myocardial infarction and identify PYK2 as a novel therapeutic target for cardioprotection PMID: 28444132
  6. microparticles from AMI patients decreased eNOS phosphorylation at Ser1177, increased eNOS phosphorylation at T495 and caveolin-1 expression, decreased eNOS association with Hsp90, decreased NO production but increased (O2) generation, damaged endothelial-dependent vasodilatation. PMID: 28002238
  7. The data of the present data provide evidence that hyperhomocysteinaemia is a vascular risk factor for erectile dysfunction by impairing cavernosa endothelial nitric oxide synthase activity. PMID: 27221552
  8. Mitochondrial dysfunction in the aging heart is accompanied by constitutive eNOS uncoupling on the background of oxidative and nitrosative stress. PMID: 29537218
  9. Expression of eNOS protein was significantly increased in Sedimentary female when compared to Sed male rats PMID: 27562066
  10. HIgh fat diet increases testicular eNOS expression and this was reduced by antioxidants. PMID: 29023053
  11. Pulmonary vascular reactivity is altered in a model of murine bronchopulmonary dysplasia, the cellular mechanisms involve a decreased eNOS phosphorylation at its activating site. PMID: 28235094
  12. these results show that coronary endothelial cells rather than cardiomyocytes play a key role in the eNOS-dependent cardioprotection of exercise. PMID: 27866931
  13. a novel mechanism and model in which CAV1 phosphorylation facilitates CAV1 scaffolding and GRK2-CAV1 interaction, thus clustering eNOS within a complex that inhibits eNOS activity, is reported. PMID: 28162981
  14. Intravenous delivery of rBMSCs expressing eNOS/F92A-Cav1 to PAH rats inhibits pulmonary vascular smooth muscle cell proliferation, and improves pulmonary haemodynamics, vascular remodelling and short-term survival. PMID: 27771236
  15. Activation of endothelial NO synthase by a xanthine derivative, KMUP-1, ameliorates hypoxia-induced apoptosis in endothelial progenitor cells. PMID: 27109251
  16. Epoetin beta pegol ameliorates flow-mediated dilation and improves endothelial nitric oxide synthase coupling state in nonobese diabetic rats. PMID: 28054454
  17. that tempol causes hypernatremia after acute sodium overload by inhibiting the thirst mechanism and facilitating diuresis, despite increasing renal eNOS expression and natriuresis PMID: 27849390
  18. results suggest that the AT1 receptor is involved in development of hypertension and cardiac fibrosis. Selective activating ACE2/eNOS and inhibiting CD44/HA interaction might be considered as the therapeutic targets for attenuating Ang II induced deleterious cardiovascular effects PMID: 27085217
  19. 3, 5, 7, 3', 4'-pentamethoxyflavone treatment of middle aged rats appeared to increase production of NO and H2S from the blood vessels by upregulating the expression of eNOS and cystathione-gamma-lyase.. PMID: 27468988
  20. Antioxidant N-acetylcysteine attenuated myocardial dysfunction and myocardial I/R injury by improving Cav-3/eNOS signaling. PMID: 27733157
  21. down-regulation of CD47 exerts a protective effect against myocardial I/R injury, which may be attributable to attenuation of oxidative stress via activation of the eNOS/nitric oxide signaling pathway. PMID: 27960187
  22. eNOS expression at mRNA and protein levels and the number of circulating EPCs were reduced significantly in rats with monocrotaline-induced pulmonary arterial hypertension. These were reduced less in rats treated with rosuvastatin, suggesting that it ameliorates remodeling by increasing the circulating EPCs and upregulating eNOS. PMID: 27655493
  23. hese results suggest that the protein adjustments observed in lipid raft/caveolin-1 microdomains could be visualized as a process required to protect the cells against NO overproduction and aberrant calpain activation. Alterations in eNOS, calpain-1 and HSP90 levels have been observed in aorta of Zucker Diabetic Rats (ZDR). PMID: 27956030
  24. role in lung-protective effects of bubble continuous positive airway pressure after extubation PMID: 26800273
  25. Combined therapy with Carthamus tinctorius L. extract and captopril in L-NAME-induced hypertensive rats normalized eNOS levels. PMID: 26938552
  26. The enhanced expression of eNOS in L-arginine treated LVH rats resulted in the amelioration of oxidative and haemodynamic parameters. PMID: 27010893
  27. This study demonstrated that enos increases in male rat with aneurysmal subarachnoid hemorrhage. PMID: 26923576
  28. Exercise managed to prevent most of the disturbances in insulin signaling caused by fructose diet (except phosphorylation of IRS1 at Tyr 632 and phosphorylation and protein expression of ERK1/2) and consequently restored function of eNOS. PMID: 26644274
  29. Combined stress decreased eNOS expression in the endothelium of microvessels and almost complete disappearance of eNOS expression in endothelial cells of the majority of capillaries. PMID: 26608376
  30. Data suggest that mild hyperglycemia in type 2 diabetes (as seen in GK rats) preserves eNOS expression in vascular endothelium and thus enhances endothelium-dependent relaxation after acute myocardial infarction preventing myocardial reperfusion injury. PMID: 26219999
  31. ZFP580 promotes not only the differentiation of EPCs into ECs by increasing the expression of eNOS and the availability of nitric oxide, but also the vessel formation in vitro and in vivo PMID: 26268592
  32. These results suggest that administration of ghrelin ameliorates impaired angiogenesis in diabetic MI rats. And these beneficial effects derive from regulating GHSR1a-mediated AMPK/eNOS signal pathway PMID: 26364514
  33. This result confirmed that Arctigenin might exert dural effects in preventing SAH-induced vasospasm through upregulating eNOS expression via the PI3K/Akt signaling pathway and attenuate endothelins after subarachnoid hemorrhage PMID: 26539501
  34. Jujuboside B increased extracellular Ca2+ influx through endothelial transient receptor potential cation (TRPC) channels, phosphorylated eNOS and promoted NO generation in vascular endothelial cells. PMID: 26901291
  35. PAR2 stimulation of NO production via an additional pathway that targets phosphorylation of Ser1177-eNOS suggests a regulatory mechanism for sustaining agonist-mediated vasodilation in metabolic syndrome. PMID: 26760532
  36. Perivascular adipose tissue in Dahl SS rats buffers vasoconstriction by activating endothelial NOS via mechanisms that may include the involvement of leptin. PMID: 26608658
  37. There was a significant reduction in eNOS expression in Metabolic Syndrome thoracic ducts suggesting that diminished NO production is partially responsible for impaired flow response. PMID: 26637560
  38. Results suggest that eNOS-mediated endoplasmic reticulum stress may participate in status epilepticus-induced vasogenic edema formation PMID: 26115585
  39. These findings provide direct evidence that in vivo I/R induces eNOS dysfunction secondary to BH4 depletion, and that pre-ischemic liposomal BH4 administration preserves eNOS function conferring cardioprotection with reduced oxidative stress PMID: 26116866
  40. Results suggest that status epilepticus-induced ETB receptor/eNOS-mediated MMP-9 activation may lead to impairments of endothelial cell function via tight junctions protein degradation PMID: 26232046
  41. Suggest that telmisartan reduced susceptibility to atrial arrhythmia to a greater extent than valsartan, ameliorated atrial remodeling, and reversed imbalances in the RAS-ERK and PI3K-Akt-eNOS pathways. PMID: 26158699
  42. miR-22 down-regulated Cav3, leading to restored eNOS activity/NO production, which inhibited cardiac myocyte apoptosis and promoted cardiac function after myocardial ischemia and reperfusion. PMID: 26191152
  43. Data suggest that activity of Nos3 in aorta endothelium can be regulated by dietary factors; here, activity of aorta Nos3 is up-regulated by high-fructose diet and further up-regulated by an antioxidant dietary supplement, epicatechin. PMID: 25943039
  44. Vasculoprotective effect of insulin after arterial injury is mediated by an eNOS-dependent mechanism. PMID: 25974101
  45. Study showed that the mRNA expression of eNOS did not increase on day 3 after the experimental spinal cord injury, with a consistent result as compared with the sham-operated and healthy control groups PMID: 25644387
  46. Data suggest that, in polycystic ovary syndrome, heart exhibits down-regulation of both eNOS (nitric oxide synthase 3) and Na+/K+-ATPase (Na+/K+ transporting ATPase alpha 1) activities, but up-regulation of iNOS (nitric oxide synthase 2) activity. PMID: 25988879
  47. Data show that resveratrol (Res) reversed caveolin-1 (Cav-1)/endothelial nitric oxide synthase (eNOS) expressions in high-fat/sucrose diet (HFS) rats. PMID: 25419974
  48. Data from in vitro studies suggest that genistein and magnesium (sometimes used as dietary supplements) enhance vasodilation of vascular smooth muscle via mechanisms involving eNOS and BK(Ca) [KCa1.1 calcium-activated potassium channel]. PMID: 25494655
  49. In response to CI-1044, activation of eNOS produces excessive levels of Nitric Oxide and vascular injury, and modulation of upstream activators of eNOS leads to regulation of eNOS activity. PMID: 24705881
  50. Suggest a potential role for ILK, the cytoskeleton and ILK signalling partners including Rho in regulating intrahepatic sinusoidal endothelial cell eNOS expression and function. PMID: 24906011
  51. Following traumatic brain injury, up-regulation of eNOS in the hippocampus occurs early after 1 hour in rats. PMID: 25224032
  52. showed that miR-27b plays a role endothelial function and NO release and elucidated a potential mechanism by which miR-27b regulates Hsp90-eNOS and NO signaling by modulating PPARgamma PMID: 25795136
  53. The upregulation of beta3-adrenoreceptor (AR) and endothelial nitric oxide synthase proteins following heart failure were also attenuated by matrine. PMID: 25322941
  54. Liraglutide may have a direct beneficial effect on diabetic nephropathy by improving eNOS activity. PMID: 25215431
  55. Single-dose rosuvastatin decreased ischemia reperfusion injury in lungs affected by pulmonary hypertension via 2 anti-inflammatory mechanisms: preserving eNOS function and inhibiting macrophage infiltration. PMID: 25454916
  56. Iodine deficiency-induced activation of ryanodine receptors leads to intracellular Ca2+ release and NO synthesis by NOS3. PMID: 25406019
  57. In angiotensin II-induced hypertension nitric oxide production is impaired in the thick ascending limbs, due to decreased NOS3 expression and altered phosphorylation. PMID: 25377910
  58. CRLI induces vascular smooth muscle relaxation and suggests a dual mechanism of eNOS activation by legume lectins via muscarinic receptors and shear stress. PMID: 25444858
  59. Data suggest that long-term dietary L-arginine supplementation up-regulates eNOS and VIP (vasoactive intestinal peptide) in mucosa and vascular endothelium of small intestine undergoing adaptation to cold temperature. PMID: 24100601
  60. Endothelial nitric oxide release is increased by enhanced eNOS activity and leads to vasorelaxation. PMID: 24469067
  61. In hyperphagia, eNOS phosphorylation is reduced in the liver. PMID: 24577062
  62. Studied exercise training effects on age-related changes in cerebral blood flow and capillary vascularity through upregulation of VEGF and eNOS. PMID: 24822184
  63. Mg attenuates RTV-mediated EC oxidative eNOS dysfunction and downregulation of hepatic eNOS expression; we suggest that Mg can serve as a beneficial adjunct therapeutic against RTV-mediated eNOS toxicity. PMID: 24434120
  64. GIT1-eNOS complex is formed and regulated. GIT1 was phosphorylated on tyrosine by Src. PMID: 24764294
  65. Direct renin inhibition protects against myocardial ischemia/reperfusion injury through activation of the PI3K-Akt-eNOS pathway. PMID: 24473199
  66. Simvastatin modulates central blood pressure control of SHRs by increasing Ras-mediated activation of the PI3K-Akt and ERK1/2-RSK signalling pathways, which then up-regulates eNOS activation. PMID: 23889671
  67. While cadmium exposures do not alter cyclic GMP, eNOS and nitrate concentrations in serum, apocynin reduces cGMP and nitrite values while significantly elevating eNOS concentrations and improving cadmium-induced anemia. PMID: 23703608
  68. Arterial function in spontaneously hypertensive rats is improved by upregulation of eNOS expression following sesamin treatment. PMID: 23624755
  69. eNOS inhibition induces and enhances cardiac fibrosis independently of blood pressure. PMID: 23863197
  70. The selective sigma1R agonist SA4503 rescues impaired endothelium-dependent vasodilation in the aorta from OVX-PO rats through sigma1R stimulation, enhancing eNOS-cGMP signaling in vascular endothelial cells. PMID: 23965801
  71. Data suggest that down-regulation of Nos3 in vascular endothelium in female (but not in male) offspring of pregnant rats with elevated blood levels of testosterone/androgen (as in pre-eclampsia) may be involved in development of hypertension. PMID: 23966325
  72. Reactive oxygen species induced by acute exercise plays an important role in activation of eNOS in thoracic aorta. PMID: 22989604
  73. These observations highlight the pivotal role that the eNOS-nitric oxide system plays in regulating the biologic response to VEGF within the glomerulus. PMID: 24063000
  74. exercise-induced cardioprotection is mainly dependent on eNOS uncoupling during early reperfusion. PMID: 24105420
  75. Telmisartan enhanced NO release by activating the PI3K/Akt system, AMPK phosphorylation and eNOS expression, which attenuated the blood pressure and oxidative stress in spontaneously hypertensive rats. PMID: 23590610
  76. Results suggest that eNOS activation is required for the neuroprotection of insulin against ischemic stroke in patients with diabetes. PMID: 23663869
  77. In intact resistance vessels, changes in flow induce NO production by transient Ca(2+)-dependent eNOS translocation from membrane to intracellular compartments and sustained Ca(2+)-independent PI3K-Akt-mediated phosphorylation. PMID: 24217770
  78. Exogenously applied caveolin-1 scaffolding domain promotes leukocyte adhesion by reduced basal endothelial nitric oxide-mediated ICAM-1 phosphorylation in rat mesenteric venules. PMID: 24043249
  79. Administration of phycocyanin in rats may ameliorate systemic blood pressure by enhancing eNOS expression in aorta that is stimulated by adiponectin. PMID: 23684441
  80. Uncoupled eNOS is responsible for Ang II-induced mesangial cell fibronectin accumulation and identifies Nox4 and mitochondrial reactive oxygen species as mediators of eNOS dysfunction. PMID: 23940049
  81. Functional deterioration of endothelial nitric oxide synthase progressed after cerebral ischemia. PMID: 23820645
  82. Fish oil blunted nicotine-induced vascular endothelial abnormalities possibly via activation of PPARgamma-eNOS-NO signals. PMID: 23208382
  83. Adipose tissue-derived stem cells attenuate acute lung injury through eNOS and eNOS-derived NO. PMID: 23563270
  84. Implicate NOS isoforms, particularly eNOS, in the altered cardiovascular autonomic control that leads to the augmented enalapril-evoked hypotension in ethanol-fed female rats. PMID: 23683529
  85. Nox4 is a critical mediator of the eNos uncoupling and decrease in nitric oxide bioavailability induced by hyperglycemia in cultured glomerular mesangial cells and in diabetes in vivo. PMID: 23816887
  86. Exercise training significantly increases eNos expression in kidney from spontaneously hypertensive and normotensive rats. PMID: 23216343
  87. Endothelial nitric oxide synthase plays a main role in producing nitric oxide in the superacute phase of hepatic ischemia prior to the upregulation of inducible nitric oxide synthase. PMID: 23485075
  88. Postconditioning activates the cGMP/PKG pathway via a mechanism independent of the PI3K/Akt cascade and dependent on the reduction of O2(-) production at the onset of reperfusion, resulting in attenuated oxidation of BH4 and reduced NOS uncoupling. PMID: 23525447
  89. These results suggest impairment of eNOS activation by phosphorylation as a potential new mechanism of cardiac endothelial dysfunction in heart disease. PMID: 23165766
  90. A short-term hypercaloric diet induces a blunted vasoconstrictive and enhanced vasodilatory phenotype in Dahl salt-sensitive rats and increased NOS3 function. PMID: 23176108
  91. 17ss-estradiol has a role in antagonizing the down-regulation of ERalpha/NOS-3 signaling in vascular endothelial dysfunction of female diabetic rats PMID: 23209733
  92. Suggest role for eNOS uncoupling in enhanced vascular superoxide, impaired endothelium-mediated vasodilatation, and decreased NO production in adult animals with programmed elevated blood pressure after a brief neonatal oxygen exposure. PMID: 23011469
  93. Akt- and PKA-mediated eNOS activation, with phosphorylation near the C-terminus, is critical for early IPC-induced cardioprotection, with eNOS-derived NO from the endothelium serving a critical role. PMID: 22977010
  94. Pharmacological depolarization of endothelial mitochondria promotes activation of eNOS by dual pathways involving increased [Ca(2+)]i as well as by phosphoinositide-3 kinase-protein kinase B-induced eNOS phosphorylation. PMID: 23329133
  95. after 6 weeks of diabetes, despite increased oxidant stress, endothelium-dependent relaxation is maintained due to the increased eNOS expression resulting in increased nitric oxide synthesis PMID: 22965470
  96. The mechanism by which NPY regulates depressor effects in the nucleus tract solitarii is demonstrated, and hypotensive effects of NPY are modulated by eNOS. PMID: 22708658
  97. Hippocampal eNOS levels correlated negatively with animals' performance but only in the inbred wistar rats. PMID: 22909987
  98. The beneficial effect of exendin-4 appears to be mediated in part via stimulation of cAMP/AMPK-related signalling pathways and enhancement of endothelial nitric oxide synthase activity. PMID: 22560150
  99. Report restorative effects of alpha1-adrenoceptor antagonists on eNOS expression in the kidney in partial bladder outlet obstruction. PMID: 22135033
  100. We evaluated whether caveolin-1 associated with eNOS/Hsp70 expression may be involved in the mechanism by which rosuvastatin exerts tubulointerstitial fibrosis protection in neonatal unilateral ureteral obstruction. PMID: 22683596

Show More

Hide All

Subcellular Location Membrane, caveola, Cytoplasm, cytoskeleton, Golgi apparatus, Cell membrane
Protein Families NOS family
Tissue Specificity Expressed constitutively by vascular endothelium. Detected in alveolar and serosal epithelial cells as well as in endothelial cells in one day old rat. In adult lung, detected in rare endothelial cells.
Database Links

KEGG: rno:24600

STRING: 10116.ENSRNOP00000013058

UniGene: Rn.44265

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1