Rat Neuroglobin,NGB ELISA Kit

Code CSB-E08738r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Rat NGB ELISA Kit was designed for the quantitative measurement of Rat NGB protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

Target Name neuroglobin
Alternative Names
Ngb ELISA Kit; Neuroglobin ELISA Kit
Abbreviation NGB
Uniprot No. Q99JA8
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat NGB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %85
Range %80-90
1:2Average %99
Range %96-102
1:4Average %93
Range %90-96
1:8Average %88
Range %84-92
The recovery of rat NGB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 104100-108
EDTA plasma (n=4)9894-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
10002.256 2.104 2.180 2.035
5001.678 1.637 1.658 1.513
2501.131 1.168 1.150 1.005
1250.742 0.765 0.754 0.609
62.50.510 0.500 0.505 0.360
31.20.416 0.401 0.409 0.264
15.60.301 0.311 0.306 0.161
00.144 0.146 0.145
ELISA Data Analysis ELISA Standard Curve & Curve Expert software
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes.
Gene References into Functions
  1. Cerebral hemorrhage significantly inhibited the spatial learning and memory ability of rats; the mechanism may be related to decreased cerebral expression of BDNF and NGB PMID: 28829495
  2. In conclusion, this study shows that EPOR and Ngb were upregulated, and both of them act as important coordinated neuroprotectors in rh-EPO treatment of PWMD. However, the two proteins exhibit different expression patterns. PMID: 28359933
  3. Ngb expression is limited to the subpopulation of type I spiral ganglion cells, those which innervate inner hair cells, while the subpopulation of type II spiral ganglion cells which innervate the outer hair cells do not express Ngb. The cochlear nuclei and superior olivary complex (SOC) also express considerable amounts of Ngb. PMID: 25636685
  4. The present study first reported the yeast expressed production of Tat-NGB-His and His-NGB-His, and then elucidated the transduction and neuroprotection of Tat-NGB-His on PC12 cell PMID: 26646387
  5. Intermittent hypobaric hypoxia preconditioning activated the expression of neuroglobin and Bcl-2 in the rat hippocampal CA1 area following ischemia-reperfusion, and prevented hippocampal neuronal apoptosis. PMID: 26400308
  7. 1-Bromopropane(1-BP)resulted in decreased endogenous neuroprotectant neuroglobin in cerebral cortex, which might play an important role in central nervous system neurotoxicity induced by 1-BP. PMID: 25557405
  8. Study demonstrated that LED light exposure up-regulated NGB expression in rat retinas; alteration was induced predominantly by blue light compared to green or red light, and increased NGB levels were correlated with the severity of retinal light damage PMID: 24747803
  9. antioxidants in severe traumatic brain injury do not seem to support the role of neuroglobin as an endogenous neuroprotective antioxidant agent PMID: 24491879
  10. These findings indicated that Ngb might play an important neuro-protective effect after subarachnoid hemorrhage. PMID: 24281943
  11. It induces neuronal amyloid beta production. PMID: 23673310
  12. involved in pathogenesis of sepsis-associated encephalopathy and has neuroprotective effects PMID: 24145836
  13. High Neuroglobin levels are associated with reactive gliosis in response to eye injury. PMID: 23639750
  14. The results of this study suggested that Ngb could be involved in sleep regulation, independent of its role in the control of oxidative stress. PMID: 23262504
  15. Up-regulation of Ngb expression played an important role in the neuroprotection induced by limb ischemic preconditioning. PMID: 23180278
  16. Ngb mRNA expression in brain is associated with oxidative stress caused by arsenite exposure in rats. PMID: 22887765
  17. Neuroglobin could protect neurons from traumatic stress and secondary ischemia and hypoxia insults during ultra-early and acute stages of acute brain injury. PMID: 20476562
  18. NGB was upregulated in traumatic brain injury PMID: 22664218
  19. we showed that the NGB expression level in neurons increased continuously from 2 h after injury, and reached a peak at 16 h after which it decreased sharply PMID: 21915648
  20. We found that NGB was present in a rat astrocytoma cell line (C6), human astrocytoma cell line (U251), and human astrocytoma tissues PMID: 22009023
  21. Data support the hypothesis that an up-regulation of neuroglobin could be a compensatory mechanism in response to the hypoxic-ischemic insults caused by seizures in pups during intrauterine life. PMID: 21767627
  22. Ngb expression quickly increases when retinal ganglion cells die in acute retina ischemia, and expression is mainly intracytoplasmic. PMID: 21054965
  23. Cygb but not Ngb may protect cerebellar cells from the chronic presence of carbon monoxide exposure during prenatal and postnatal development. PMID: 20230802
  24. Neuroglobin expression was decreasd in the cochlea of rat pups exposed to chronic very mild carbon monoxide in air during and after the prenatal period. PMID: 20211612
  25. results indicate that the Ngb could be involved in both photic and nonphotic entrainment via input from the geniticulo-hypothalamic tract PMID: 20187147
  26. Up-regulation of neuroglobin expression in brain injury induced by LPS is correlated with duration after challenge of LPS. PMID: 19842562
  27. NGB mRNA and protein are abundantly expressed and widely distributed throughout the adult rat brain, as seen in the NGB-immunopositive signals of neuronal cell bodies and processes. PMID: 11820779
  28. acts as an endogenous neuroprotective factor in focal cerebral ischemia PMID: 12621155
  29. Examination of the cell type-specific expression of neuroglobin in neurons and astroglial cells in primary cultures of rat fetal hippocampal cells finds neuroglobin is only expressed in neurons. PMID: 15193759
  30. is present in the carotid body and increases after chronic intermittent hypoxia PMID: 16683692
  31. hypoxic stimulus presentation is a major determinant of the regulation of hypoxic sensitive genes such as Ngb PMID: 16750520
  32. it is unlikely that neuroglobin is instrumental in the acute response of neurons to hypoxic or ischemic insults PMID: 16796995
  33. Therefore, Ngb may act as an intracellular ROS scavenger, and such antioxidant properties may play a protective role against Abeta-induced cell injury. PMID: 17560688
  34. Recombinant neuroglobin is fused to human HIV-1 transactivator-of-transcription protein-transduction domain (TAT[49-57] PTD), enters primary cultured cortical neurons in a dose-dependent manner and protects the neurons from hypoxia-induced apoptosis. PMID: 17576199
  35. A diurnal variation of Ngb mRNA in the suprachiasmatic nucleus, having a peak in the day time (light-period) and nadir during night (dark-period). PMID: 18451642
  36. The cellular and physiological function of Ngb in different subsets of neurones might not be identical. PMID: 18509243
  37. Results demonstrate by immunohistochemical analysis the colocalization of neuroglobin and PrP(c) in the retinal ganglion cell layer. PMID: 19327369
  38. the role of Ngb during aging and hypoxia PMID: 19536481

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Subcellular Location Perikaryon.
Protein Families Globin family
Tissue Specificity Widely distributed throughout the adult brain, including cerebral cortex, hippocampus, thalamus, hypothalamus, olfactory bulb, and cerebellum.
Database Links

KEGG: rno:85382

STRING: 10116.ENSRNOP00000016057

UniGene: Rn.64645

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