Human Interleukin 27,IL-27 ELISA Kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Alternative Names

EBI3 ELISA Kit; IL 27 ELISA Kit; IL 27 p28 subunit ELISA Kit; IL 27A ELISA Kit; IL 30 ELISA Kit; IL-27 subunit alpha ELISA Kit; IL-27-A ELISA Kit; IL27 ELISA Kit; IL27-A ELISA Kit; IL27A ELISA Kit; IL27A_HUMAN ELISA Kit; IL27p28 ELISA Kit; IL30 ELISA Kit; Interleukin 27 ELISA Kit; Interleukin 27 subunit alpha ELISA Kit; Interleukin 27; 28-KD subunit ELISA Kit; Interleukin 30 ELISA Kit; Interleukin-27 subunit alpha ELISA Kit; p28 ELISA Kit

Abbreviation

IL27

Uniprot No.

Q8NEV9

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.156 ng/ml - 10 ng/ml

Sensitivity

0.039 ng/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	89
	Range %	84-95
1:4	Average %	92
	Range %	87-96
1:8	Average %	97
	Range %	92-104

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.815

2.869

2.842

2.717

2.436

2.647

2.542

2.417

2.5

1.954

1.968

1.961

1.836

1.25

1.312

1.336

1.324

1.199

0.625

0.764

0.704

0.734

0.609

0.312

0.468

0.442

0.455

0.330

0.156

0.297

0.274

0.286

0.161

0.124

0.126

0.125

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This human IL-27 ELISA Kit is suitable for qualitatively determining human concentrations in multiple biological fluids, including human serum, plasma, and tissue homogenates in vitro.IL27 is a heterodimeric cytokine that plays a role both in innate and adaptive immunity. IL27 engages the IL27 receptor and then recruits several Jak family kinases, activating the JAK-STAT pathway, thus mediating many cellular processes, including cellular differentiation, proliferation, and immunologic responses. Studies have shown that IL27 regulates T cell responses that prevent immune hyperactivity, suggesting a possible therapeutic for chronic inflammatory conditions with excessive T cell activation, such as MS and rheumatoid arthritis. Contrastly, IL27 exerts pro-inflammatory functions by promoting the clonal expansion of naive CD4+ T-cells and the survival of Treg and CD8+ T cells and stimulating tumor-specific cytotoxic T cell responses.

This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human IL-27 in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-human IL-27 antibody. Biotin-labeled IL-27 antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the IL-27 in the human samples. IL-27 binds to the biotinylated anti-IL-27 human monoclonal antibody. And the biotin on the biotinylated anti-IL-27 human monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of IL-27 in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.

Target Background

Function
(From Uniprot)

Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication.

Gene References into Functions

Over-expression of IL-17 and IL-27 are involved in the pathogenesis of liver damage in children with human cytomegalovrius infection. PMID: 30022757
Interleukin-27 differentially regulates the expression of seven novel microRNAs. PMID: 29343703
Patients with tuberculous pleural effusion have an about 26-fold higher chance of being IL-27 test-positive compared to patients with malignant pleural effusion. [meta-analysis] PMID: 29539604
there was a significant increase in the risk of Kawasaki disease with coronary arterial lesions in children with the rs17855750 (T>G), rs40837 (A>G), rs4740 (G>A), rs4905 (A>G), rs153109 (T>C), and rs26528 (A>G) Single-nucleotide Polymorphisms. PMID: 29973303
our results showed that SNP rs153109 displayed significant associations with dilated cardiomyopathy in Chinese Han population, whereas no differences in genotype or allele frequencies were found between dilated cardiomyopathy patients and controls at SNP rs17855750. PMID: 29754565
Human Immunodeficiency virus type-1 myeloid derived suppressor cells inhibit cytomegalovirus inflammation through IL-27 and B7-H4. PMID: 28338007
IL-27 enhances the potential of reactive oxygen species generation from monocyte-derived macrophages and dendritic cells by induction of p47(phox). PMID: 28240310
IL-27 has a unique ability to directly act on hematopoietic stem cells and promote their expansion and differentiation into myeloid progenitors in hematologic neoplasms and malaria. (Review) PMID: 29218601
IL-27 rs153109 and rs17855750 Polymorphisms are associated with Risk and Response to Therapy in Acute Lymphoblastic Leukemia. PMID: 28828696
IL-27 was down-regulated in the malignant Middle Cerebral Artery Infarction. PMID: 28828208
IL-27 may be involved in the development of coronary artery lesions in acute Kawasaki disease. PMID: 28108813
Hepatitis B virus infection can lead to overexpression of interleukin-27 gene in liver transplant patients. PMID: 26925776
this study indicates that IL-27 secreted by cervical carcinoma cells restricts the angiogenesis in a paracrine manner in the pathogenesis of cervical cancer PMID: 28508429
IL-27 signaling (IL-27 and its receptor) may be involved in the pathogenesis of HIV infection and immune reconstitution in HIV/AIDS patients who underwent HAART. IL-27 may exert effects through regulating Th1 / Th2 ratio. PMID: 28521720
This study provides additional evidence that IL-27 may contribute to autoimmune processes in MS patients. PMID: 28738904
this study shows that IL-27 regulates the adherence, proliferation, and migration of placenta-derived mesenchymal stromal cells and enhances their regulatory effects on Th1 and Th2 subset generations PMID: 28612255
This study failed to find an association between common variants in the functional region of IL27 and coronary artery disease in a Chinese Han population PMID: 27174010
Data point out to a broad set of activities shared by IL-27 and IFN-gamma, which are dependent on the common activation of the STAT1 pathway. These data add further explanation to the anti-tumor activity of IL-27 and also to its dual role in immune regulation PMID: 27683036
The current meta-analysis suggests that IL-27 -964A/G polymorphism might enhance cancer risk. However, large-scale and well-designed studies are still needed to confirm the result of our meta-analysis PMID: 25424605
this study found elevated expression of IL-27 in human peripheral serum and elevated expression of its specific receptor (wsx-1) on fetal membranes in cases of preterm birth PMID: 28756371
results indicate that elevated IL-27 strongly correlates with EOS and may provide additional diagnostic value along with PCT. PMID: 27648693
serum interleukin-27 and interleukin-35 concentrations in patients with Guillain-Barre syndrome PMID: 28167198
Expression of IL-27 in the serum was markedly increased in patients with sepsis-induced myocardial dysfunction compared with that in controls. PMID: 27525353
IL-27 significantly inhibited the M2 macrophages polarization and dampened the proliferation, migration and metastasis of pancreatic cancer cells and as well enhanced the efficacy of gemcitabine. PMID: 26868086
IL-27 increased NLRP3 inflammasome activation in monocytes with enhanced release of IL-1 beta. PMID: 29176764
this study shows that interleukin 27 is up-regulated in patients with active inflammatory bowel disease PMID: 27221243
IL-27 polymorphisms might play a protective role for the development of ulcerative colitis in the Mexican population PMID: 26905929
IL-27 stimulation activated STAT1 and STAT3 in HeLa and T98G cells, suggesting that IL-27 engages the STAT1/3 pathway, which then leads to the upregulation of IL-6, IP-10, and MIG PMID: 27617644
IL-27 and IL-37 limit the induction of particular T cell subsets along with cytokine responses in S. stercoralis infections, which suggest the importance of IL-27 and IL-37 in immune modulation in a chronic helminth infection. PMID: 28874444
IL-27 is a pivotal regulator in endometriotic immune tolerance by triggering Th17 cells to produce IL-10. PMID: 28300844
Significantly increased papillary thyroid carcinoma risk was also associated with rs17855750 GT and GT/GG genotypes. PMID: 27929666
HIV and Tat inhibit LPS-induced IL-27 production in human macrophages via distinct mechanisms: Tat through the inhibition of cIAP-1-TRAF-6 and subsequent inhibition of PI3K and p38 MAPKs, whereas HIV through the inhibition of p38 MAPK activation. PMID: 28698313
IL27 recapitulates IFN-gamma responses in hepatocellular carcinoma cells, but is inhibited by IL6-type cytokines. PMID: 27939431
These findings provide new evidence that IL-27 plays an important role in the proinflammatory capacity of monocytes and macrophages via enhancing IL-1beta secretion levels triggered by dual LPS-ATP stimulation. PMID: 28377398
IL-27 enhanced the cytotoxicity and suppressed the proliferation of ovarian cancer cells PMID: 27639718
the sIL-6R/p28 fusion protein (FP) upregulated IFN-lambda1 expression by increasing the binding abilities of c-Fos and activating transcription factor 1 to the IFN-lambda1 promoter via the p38 MAPK signaling pathway; results demonstrate the important role of sIL-6R/p28 FP in mediating virus-induced type III IFN production. PMID: 27527594
These results identify IL-27 and its specific receptor IL27RA as a critical immune axis for the antiviral immune response and as robust correlates of viral load and proviral reservoir size in mononuclear leukocytes in HIV infection. PMID: 28592538
IL30 regulates breast cancer cell viability, migration, and gene expression to promote breast cancer growth and progression and its impact on patient outcome. PMID: 27550449
serum IL-27 levels were significantly higher in stage III EV71-infected hand, foot, and mouth disease patients with early cardiopulmonary failure and strong correlation with CRP levels; IL27p28 polymorphisms (rs153109, rs17855750, and rs181206) did not influence IL-27 production, and these three SNPs were not associated with EV71 infection risk and clinical stage PMID: 27403033
these results suggest that IL-27 might predominantly play a protective role in the pathogenesis of psoriasis through abrogating Th17 differentiation. PMID: 27939414
our data suggest a previously unrecognized role for IL-27 in regulating epithelial cell proliferation and antiviral host defense during the normal wound healing response. PMID: 28132857
An association between the IL-27 single nucleotide polymorphism (SNP) rs153109 and Atrial Fibrillation was found in Chinese Han population. The G allele and GG genotype of rs153109 were associated with increased AF risk (odds ratio [OR]: 1.35, 95% confidence intervals [CI]=1.06-1.71, p=0.02 and OR: 1.66, 95% CI=1.03-2.65, p=0.03 in the recessive genetic model, respectively) PMID: 28055231
Study demonstrated that levels of circulating serum IL-27 were elevated in rheumatoid arthritis (RA) patients and were positively correlated with RA disease activity. Moreover, these concentrations decreased after effective immunosuppressant treatment suggesting its involvement in the pathogenesis of RA. PMID: 27869736
Data indicate that the genetic variant rs153109 in the 5' upstream region of interleukin 27 (IL-27) is significantly associated with ulcerative colitis (UC) in Chinese Han individuals. PMID: 28069403
this study shows that the serum levels of IL-27 are lower in Iranian diabetes type I patients compared to the healthy individuals PMID: 27611173
our findings showed a relationship between the IL-27 2905T/G genotype variant and the decreased susceptibility for developing cervical cancer. The IL-27 2905T/G genotype variant also modified the survival time in cervical cancer patients. PMID: 26662568
an IL-27/Stat3 axis induces expression of programmed cell death 1 ligands (PD-L1/2) on infiltrating macrophages in lymphoma PMID: 27564404
Interleukin-27 rs 153109 Single Nucleotide Polymorphism is associated with responses to therapy in Hepatitis C. PMID: 27221901
this study shows that IL-27 gene polymorphism is genetic risk factor for systemic lupus erythematosus in the Polish population, and that it predicts disease phenotype PMID: 27059274
Data indicate that the CC genotype of interleukin 27 (IL-27) single nucleotide polymorphism rs153109 was significantly more prevalent in pre-eclampsia (PE) cases than controls. PMID: 26971578

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Subcellular Location

Secreted. Note=Does not seem to be secreted without coexpression of EBI3.

Protein Families

IL-6 superfamily

Tissue Specificity

Expressed in monocytes and in placenta.

Database Links

HGNC: 19157

OMIM: 608273

KEGG: hsa:246778

STRING: 9606.ENSP00000349365

UniGene: Hs.528111

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Code	CSB-E08464h
Size	$496
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.