Name: Recombinant Human Interleukin-27 subunit alpha(IL27)
Brand: CUSABIO
SKU: 847702HU

Product Details

Purity	>85% (SDS-PAGE)
Target Names	IL27
Uniprot No.	Q8NEV9
Alternative Names	EBI3; IL 27; IL 27 p28 subunit; IL 27A; IL 30; IL-27 subunit alpha; IL-27-A; IL27; IL27-A; IL27A; IL27A_HUMAN; IL27p28; IL30; Interleukin 27; Interleukin 27 subunit alpha; Interleukin 27; 28-KD subunit; Interleukin 30; Interleukin-27 subunit alpha; p28
Species	Homo sapiens (Human)
Expression Region	29-243
Target Protein Sequence	FP RPPGRPQLSL QELRREFTVS LHLARKLLSE VRGQAHRFAE SHLPGVNLYL LPLGEQLPDV SLTFQAWRRL SDPERLCFIS TTLQPFHALL GGLGTQGRWT NMERMQLWAM RLDLRDLQRH LRFQVLAAGF NLPEEEEEEE EEEEEERKGL LPGALGSALQ GPAQVSWPQL LSTYRLLHSL ELVLSRAVRE LLLLSKAGHS VWPLGFPTLS PQP
Protein Length	Full Length of Mature Protein
Tag Info	The following tags are available. N-terminal His-tagged Tag-Free The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form	Lyophilized powder
Buffer before Lyophilization	Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet	Please contact us to get it.

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Target Data

Function	Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication.
Gene References into Functions	Over-expression of IL-17 and IL-27 are involved in the pathogenesis of liver damage in children with human cytomegalovrius infection. PMID: 30022757 Interleukin-27 differentially regulates the expression of seven novel microRNAs. PMID: 29343703 Patients with tuberculous pleural effusion have an about 26-fold higher chance of being IL-27 test-positive compared to patients with malignant pleural effusion. [meta-analysis] PMID: 29539604 there was a significant increase in the risk of Kawasaki disease with coronary arterial lesions in children with the rs17855750 (T>G), rs40837 (A>G), rs4740 (G>A), rs4905 (A>G), rs153109 (T>C), and rs26528 (A>G) Single-nucleotide Polymorphisms. PMID: 29973303 our results showed that SNP rs153109 displayed significant associations with dilated cardiomyopathy in Chinese Han population, whereas no differences in genotype or allele frequencies were found between dilated cardiomyopathy patients and controls at SNP rs17855750. PMID: 29754565 Human Immunodeficiency virus type-1 myeloid derived suppressor cells inhibit cytomegalovirus inflammation through IL-27 and B7-H4. PMID: 28338007 IL-27 enhances the potential of reactive oxygen species generation from monocyte-derived macrophages and dendritic cells by induction of p47(phox). PMID: 28240310 IL-27 has a unique ability to directly act on hematopoietic stem cells and promote their expansion and differentiation into myeloid progenitors in hematologic neoplasms and malaria. (Review) PMID: 29218601 IL-27 rs153109 and rs17855750 Polymorphisms are associated with Risk and Response to Therapy in Acute Lymphoblastic Leukemia. PMID: 28828696 IL-27 was down-regulated in the malignant Middle Cerebral Artery Infarction. PMID: 28828208 IL-27 may be involved in the development of coronary artery lesions in acute Kawasaki disease. PMID: 28108813 Hepatitis B virus infection can lead to overexpression of interleukin-27 gene in liver transplant patients. PMID: 26925776 this study indicates that IL-27 secreted by cervical carcinoma cells restricts the angiogenesis in a paracrine manner in the pathogenesis of cervical cancer PMID: 28508429 IL-27 signaling (IL-27 and its receptor) may be involved in the pathogenesis of HIV infection and immune reconstitution in HIV/AIDS patients who underwent HAART. IL-27 may exert effects through regulating Th1 / Th2 ratio. PMID: 28521720 This study provides additional evidence that IL-27 may contribute to autoimmune processes in MS patients. PMID: 28738904 this study shows that IL-27 regulates the adherence, proliferation, and migration of placenta-derived mesenchymal stromal cells and enhances their regulatory effects on Th1 and Th2 subset generations PMID: 28612255 This study failed to find an association between common variants in the functional region of IL27 and coronary artery disease in a Chinese Han population PMID: 27174010 Data point out to a broad set of activities shared by IL-27 and IFN-gamma, which are dependent on the common activation of the STAT1 pathway. These data add further explanation to the anti-tumor activity of IL-27 and also to its dual role in immune regulation PMID: 27683036 The current meta-analysis suggests that IL-27 -964A/G polymorphism might enhance cancer risk. However, large-scale and well-designed studies are still needed to confirm the result of our meta-analysis PMID: 25424605 this study found elevated expression of IL-27 in human peripheral serum and elevated expression of its specific receptor (wsx-1) on fetal membranes in cases of preterm birth PMID: 28756371 results indicate that elevated IL-27 strongly correlates with EOS and may provide additional diagnostic value along with PCT. PMID: 27648693 serum interleukin-27 and interleukin-35 concentrations in patients with Guillain-Barre syndrome PMID: 28167198 Expression of IL-27 in the serum was markedly increased in patients with sepsis-induced myocardial dysfunction compared with that in controls. PMID: 27525353 IL-27 significantly inhibited the M2 macrophages polarization and dampened the proliferation, migration and metastasis of pancreatic cancer cells and as well enhanced the efficacy of gemcitabine. PMID: 26868086 IL-27 increased NLRP3 inflammasome activation in monocytes with enhanced release of IL-1 beta. PMID: 29176764 this study shows that interleukin 27 is up-regulated in patients with active inflammatory bowel disease PMID: 27221243 IL-27 polymorphisms might play a protective role for the development of ulcerative colitis in the Mexican population PMID: 26905929 IL-27 stimulation activated STAT1 and STAT3 in HeLa and T98G cells, suggesting that IL-27 engages the STAT1/3 pathway, which then leads to the upregulation of IL-6, IP-10, and MIG PMID: 27617644 IL-27 and IL-37 limit the induction of particular T cell subsets along with cytokine responses in S. stercoralis infections, which suggest the importance of IL-27 and IL-37 in immune modulation in a chronic helminth infection. PMID: 28874444 IL-27 is a pivotal regulator in endometriotic immune tolerance by triggering Th17 cells to produce IL-10. PMID: 28300844 Significantly increased papillary thyroid carcinoma risk was also associated with rs17855750 GT and GT/GG genotypes. PMID: 27929666 HIV and Tat inhibit LPS-induced IL-27 production in human macrophages via distinct mechanisms: Tat through the inhibition of cIAP-1-TRAF-6 and subsequent inhibition of PI3K and p38 MAPKs, whereas HIV through the inhibition of p38 MAPK activation. PMID: 28698313 IL27 recapitulates IFN-gamma responses in hepatocellular carcinoma cells, but is inhibited by IL6-type cytokines. PMID: 27939431 These findings provide new evidence that IL-27 plays an important role in the proinflammatory capacity of monocytes and macrophages via enhancing IL-1beta secretion levels triggered by dual LPS-ATP stimulation. PMID: 28377398 IL-27 enhanced the cytotoxicity and suppressed the proliferation of ovarian cancer cells PMID: 27639718 the sIL-6R/p28 fusion protein (FP) upregulated IFN-lambda1 expression by increasing the binding abilities of c-Fos and activating transcription factor 1 to the IFN-lambda1 promoter via the p38 MAPK signaling pathway; results demonstrate the important role of sIL-6R/p28 FP in mediating virus-induced type III IFN production. PMID: 27527594 These results identify IL-27 and its specific receptor IL27RA as a critical immune axis for the antiviral immune response and as robust correlates of viral load and proviral reservoir size in mononuclear leukocytes in HIV infection. PMID: 28592538 IL30 regulates breast cancer cell viability, migration, and gene expression to promote breast cancer growth and progression and its impact on patient outcome. PMID: 27550449 serum IL-27 levels were significantly higher in stage III EV71-infected hand, foot, and mouth disease patients with early cardiopulmonary failure and strong correlation with CRP levels; IL27p28 polymorphisms (rs153109, rs17855750, and rs181206) did not influence IL-27 production, and these three SNPs were not associated with EV71 infection risk and clinical stage PMID: 27403033 these results suggest that IL-27 might predominantly play a protective role in the pathogenesis of psoriasis through abrogating Th17 differentiation. PMID: 27939414 our data suggest a previously unrecognized role for IL-27 in regulating epithelial cell proliferation and antiviral host defense during the normal wound healing response. PMID: 28132857 An association between the IL-27 single nucleotide polymorphism (SNP) rs153109 and Atrial Fibrillation was found in Chinese Han population. The G allele and GG genotype of rs153109 were associated with increased AF risk (odds ratio [OR]: 1.35, 95% confidence intervals [CI]=1.06-1.71, p=0.02 and OR: 1.66, 95% CI=1.03-2.65, p=0.03 in the recessive genetic model, respectively) PMID: 28055231 Study demonstrated that levels of circulating serum IL-27 were elevated in rheumatoid arthritis (RA) patients and were positively correlated with RA disease activity. Moreover, these concentrations decreased after effective immunosuppressant treatment suggesting its involvement in the pathogenesis of RA. PMID: 27869736 Data indicate that the genetic variant rs153109 in the 5' upstream region of interleukin 27 (IL-27) is significantly associated with ulcerative colitis (UC) in Chinese Han individuals. PMID: 28069403 this study shows that the serum levels of IL-27 are lower in Iranian diabetes type I patients compared to the healthy individuals PMID: 27611173 our findings showed a relationship between the IL-27 2905T/G genotype variant and the decreased susceptibility for developing cervical cancer. The IL-27 2905T/G genotype variant also modified the survival time in cervical cancer patients. PMID: 26662568 an IL-27/Stat3 axis induces expression of programmed cell death 1 ligands (PD-L1/2) on infiltrating macrophages in lymphoma PMID: 27564404 Interleukin-27 rs 153109 Single Nucleotide Polymorphism is associated with responses to therapy in Hepatitis C. PMID: 27221901 this study shows that IL-27 gene polymorphism is genetic risk factor for systemic lupus erythematosus in the Polish population, and that it predicts disease phenotype PMID: 27059274 Data indicate that the CC genotype of interleukin 27 (IL-27) single nucleotide polymorphism rs153109 was significantly more prevalent in pre-eclampsia (PE) cases than controls. PMID: 26971578 Show More Hide All
Subcellular Location	Secreted
Protein Families	IL-6 superfamily
Tissue Specificity	Expressed in monocytes and in placenta.
Database Links	HGNC: 19157 OMIM: 608273 KEGG: hsa:246778 STRING: 9606.ENSP00000349365 UniGene: Hs.528111

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Code	CSB-YP847702HU
Size	Pls inquire
Source	Yeast
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Code	CSB-EP847702HU
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Source	E.coli
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Code	CSB-EP847702HU-B
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Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP847702HU
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Source	Baculovirus
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Code	CSB-MP847702HU
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Source	Mammalian cell
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Recombinant Human Interleukin-27 subunit alpha(IL27)

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