rat follicle-stimulating hormone,FSH ELISA Kit

Code CSB-E06869r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T
Uniprot No. P18427
Lead Time 3-5 working days
Abbreviation FSH
Target Name follicle-stimulating hormone,FSH
Alias N/A
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.3 mLU/mL-10 mLU/mL
Sensitivity 0.07 mLU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
 


 
Linearity To assess the linearity of the assay, samples were spiked with high concentrations of rat FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.












Recovery The recovery of rat FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.




Typical Data These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



References
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Function Stimulates development of follicle and spermatogenesis in the reproductive organs.
Subcellular Location Secreted
Protein Families Glycoprotein hormones subunit beta family
Database Links

KEGG: rno:25447

STRING: 10116.ENSRNOP00000006512

UniGene: Rn.92303

Pathway cAMP signaling pathway

Q&A and Customer Reviews

Q&A
Q:


1) What is the calibrator?
2) Is the calibration traceable to the RP-2 rat FSH?

A:
Thanks for your inquiry. CSB-E06869r is designed for common rat and its standard is 293 cell-derived recombinant rat FSH protein.
Customer Reviews

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