rat follicle-stimulating hormone,FSH ELISA Kit

Code CSB-E06869r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The Rat follicle-stimulating hormone (FSH) ELISA Kit is designed for the quantitative determination of endogenic rat FSH concentrations in serum, plasma, or tissue homogenates. This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FSH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Alternative Names FshbFollitropin subunit beta ELISA kit; Follicle-stimulating hormone beta subunit ELISA kit; FSH-B ELISA kit; FSH-beta ELISA kit; Follitropin beta chain ELISA kit
Abbreviation FSH
Uniprot No. P18427
Species Rattus norvegicus (Rat)
Sample Types
Detection Range
Sensitivity
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Materials provided
    • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit FSH antibody.
    • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • Anti-rat FSH Antibody 1 x 6 ml -- Act as the detection antibody.
    • HRP-conjugated FSH 1 x 6 ml -- Bind to the FSH antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
    • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
    • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
    • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
    • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

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 Q&A
Q:


1) What is the calibrator?
2) Is the calibration traceable to the RP-2 rat FSH?

A:
Thanks for your inquiry. CSB-E06869r is designed for common rat and its standard is 293 cell-derived recombinant rat FSH protein.

Target Background

Function
(From Uniprot)
Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
Gene References into Functions
  1. Study in primary rat pituitary cells demonstrates that miR-186-5p regulates FSH secretion by directly targeting the FSHb 3' UTR. PMID: 29534107
  2. our results indicate that miR-21-3p and miR-433 can down-regulate the expression of FSHb by directly targeting the FSHb 3'UTR in rat primary pituitary cells. PMID: 28402262
  3. the upstream region of Fshb is susceptible to unsaturated long-chain fatty acids PMID: 26853521
  4. GnRH stimulation of Fshb expression is dependent on miR-132/212 and involves a SIRT1-FOXO1 pathway. PMID: 25635942
  5. FSH acutely regulated CCND2 expression through both PKA and PI3K signaling pathways during granulosa cell proliferation and also accelerated its ubiquitination-proteasomal degradation. PMID: 23349233
  6. Data suggest that FSH (Cga/FSHb) induces clock system in granulosa cells by up-regulation of connexin 43 expression; this suggests that gap junctions are implicated in circadian clock in peripheral oscillators besides central pacemaker. PMID: 23299500
  7. Both the hormonal milieu of perimenopause and the association of dynamic perimenopausal changes in FSH and inhibin A with decreased bone mineral density have been reproduced in an animal model. PMID: 22249524
  8. FSH autoantibody could cause the reduction of inhibin B, thereby inducing hypospermatogenesis via augment of spermatogenic cell apoptosis PMID: 22564408
  9. Novel findings supporting the role of FSH in primordial follicle survival through an action on apoptosis. PMID: 22106407
  10. These results show that the synthesis of FSH isoforms possessing alpha2,6-linked sialic acid is hormonally regulated in male rats. PMID: 19464343
  11. Data suggest that FSH beta subunit expression (and LH beta subunit expression) is regulated by DUSP1 expression (specifically in response to high-frequency GnRH pulses); this effect may occur in association with MAPK3/1 phosphorylation. PMID: 21228211
  12. A potential new regulatory mechanism of the FSH signalling pathway that could occur during maturation of testicular cells. PMID: 20201104
  13. Pituitary homeobox 1 activates the rat FSHbeta (rFSHbeta) gene through both direct and indirect interactions with the rFSHbeta gene promoter. PMID: 12145338
  14. The regulation of FSHbeta is dependent on activin-mediated signaling factors in concert with pituitary-derived nuclear regulatory proteins. PMID: 12554780
  15. PACAP contributes to the selective regulation of FSHbeta expression during maturation in the male PMID: 12646491
  16. androgens acting directly on the pituitary increase FSH-beta and decrease LH-beta transcription. PMID: 14512439
  17. FSH enhances HIF-1 activity downstream of the PI 3-kinase/AKT/Rheb/mTOR pathway in GCs and HIF-1 activity is necessary for FSH to induce multiple follicular differentiation markers PMID: 14982927
  18. SMAD3 plays an important role in the synergistic effects of activin and gonadotropin-releasing hormone and demonstrate that this synergy is mediated by a palindromic cis-element located at -266/-259 of the rFSHbeta gene promoter PMID: 15375186
  19. Smad3 is not only sufficent but also necessary for activin-mediated regulation of FSHbeta promoter. PMID: 15761025
  20. oxidative stress induces apoptosis in preovulatory follicles and the antiapoptotic effect of FSH is mediated in part by stimulation of follicular GSH synthesis and suppression of ROS production PMID: 16339198
  21. Testosterone regulates FSHbeta transcription, in part, through modulation of various components of the activin-signaling system. PMID: 17405825
  22. The result found that pyramidal neurons from CA1 to CA4 region and granule neurons in dentate gyrus could express FSH and its receptor, majority of hippocampal neurons co-expressed FSH and its receptor, FSH and GnRH receptor. PMID: 17674129
  23. Smad3 and Pitx2 cooperate in stimulation of FSHbeta gene transcription. PMID: 18022758
  24. PI3-kinase may be involved in the negative regulation of alpha- and FSHbeta-subunit gene expression as well as cell proliferation. PMID: 18206295
  25. Dexamethasone (4 nM) for 3 weeks partially rescued LH beta/follicle-stimulating hormone beta cells. PMID: 18363807

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Subcellular Location Secreted.
Protein Families Glycoprotein hormones subunit beta family
Database Links

KEGG: rno:25447

STRING: 10116.ENSRNOP00000006512

UniGene: Rn.92303

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