CYC1 Monoclonal Antibody

Datasheet

Code	CSB-MA006327A0m
Size	US$210
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Image	Western Blot Positive WB detected in: CYC1 antibody at 1:1000 Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: U251 whole cell lysate Lane 6: A549 whole cell lysate Lane 7: NIH/3T3 whole cell lysate Lane 8: PC-3 whole cell lysate Secondary Goat polyclonal to Mouse IgG at 1/20000 dilution Predicted band size: 35 KDa Observed band size: 35 KDa Exposure time: 5min Overlay Peak curve showing HepG2 cells stained with CSB-MA006327A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Full Product Name

Mouse anti-Homo sapiens (Human) CYC1 Monoclonal antibody

Uniprot No.

P08574

Target Names

CYC1

Alternative Names

Cytochrome c1, heme protein, mitochondrial (EC:7.1.1.8); Complex III subunit 4; Complex III subunit IV; Cytochrome b-c1 complex subunit 4; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; Cytochrome c-1

Raised in

Mouse

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Cytochrome c1 protein (85-325AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG1

Clone No.

27E12D11

Purification Method

>95%, Protein A purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Mice were immunized with the recombinant human CYC1 protein (85-325aa), followed by the isolation of B cells from the mouse spleen, the fusion of these B cells with myeloma cells, and the selection of hybridomas that produce CYC1 antibodies. The CYC1 antibody-generating hybridomas were cultured to collect the CYC1 monoclonal antibodies. The CYC1 monoclonal antibody specifically reacts with human and mouse species and is suitable for ELISA, WB, and FC applications. The antibody is purified using protein A affinity chromatography and has a purity of over 95%.

CYC1 plays a crucial role in cellular energy metabolism and cell death regulation. It participates in the transfer of electrons between complexes III and IV of the respiratory chain, which drives the proton gradient across the mitochondrial inner membrane necessary for ATP synthesis. CYC1 also regulates apoptosis, or programmed cell death, by modulating the release of cytochrome c from mitochondria, which triggers the activation of caspases and leads to cell death.

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Target Background

Function

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. Cytochrome c1 is a catalytic core subunit containing a c-type heme. It transfers electrons from the [2Fe-2S] iron-sulfur cluster of the Rieske protein to cytochrome c.

Gene References into Functions

these results indicate that CYC1 plays important roles in cell proliferation and comedo necrosis through the elevated oxidative phosphorylation activity in human ductal carcinoma in situ PMID: 28394473
results indicate that miR-661 plays a tumor suppressor role in OS mediated by the downregulation of CYC1, suggesting a potential mechanism underlying cell death resistance in Osteosarcoma. PMID: 28391262
CYC1 promoted tumor metastasis via suppressing activation of AMPK and contributed to tumor growth via facilitating production of ATP. PMID: 27239088
FASL, granzyme B, and cytochrome c blood expression reflects breast cancer progression and response to therapy. (Review) PMID: 27117663
our results provide evidence that CYC1 plays an important role in OS tumorigenesis, and modulation of CYC1 may be an effective strategy to potentiate OS to apoptotic induction by TRAIL. PMID: 25562155
Mutations in CYC1 cause insulin-responsive hyperglycemia. PMID: 23910460
HeLa cells expressing non-cleavable cytochrome c1 maintain mitochondrial morphology and functions. PMID: 21577235
Decreased electron Transport Complex IV activity is associated with ulcerative colitis. PMID: 20440543
Addition of H202 to tendon fibroblasts in vitro resulted in oxidative stress-induced apoptosis and the release of CYC1. PMID: 12788227
the Ca(2+) spike, mitochondrial Bcl-2 presensitization, and subsequent Delta psi(m) transition, Bax translocation, and ROS generation are important upstream signals for cytochrome c release upon stimulation by a Bcl-2 inhibitor. PMID: 14966123
Sensitivity of Cyt c detection in -linked immunosorbent assay is reduced in the presence of serum leucine-rich alpha-2-glycoprotein-1. PMID: 16699948

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Involvement in disease

Mitochondrial complex III deficiency, nuclear 6 (MC3DN6)

Subcellular Location

Mitochondrion inner membrane; Single-pass membrane protein.

Protein Families

Cytochrome c family

Database Links

HGNC: 2579

OMIM: 123980

KEGG: hsa:1537

STRING: 9606.ENSP00000317159

UniGene: Hs.289271

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Cardiac muscle contraction Oxidative Phosphorylation

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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