CYC1 Monoclonal Antibody

Code CSB-MA006327A0m
Size US$210
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Image
  • Western Blot
    Positive WB detected in: CYC1 antibody at 1:1000
    Lane 1: Hela whole cell lysate
    Lane 2: HepG2 whole cell lysate
    Lane 3: MCF-7 whole cell lysate
    Lane 4: K562 whole cell lysate
    Lane 5: U251 whole cell lysate
    Lane 6: A549 whole cell lysate
    Lane 7: NIH/3T3 whole cell lysate
    Lane 8: PC-3 whole cell lysate
    Secondary
    Goat polyclonal to Mouse IgG at 1/20000 dilution
    Predicted band size: 35 KDa
    Observed band size: 35 KDa
    Exposure time: 5min
  • Overlay Peak curve showing HepG2 cells stained with CSB-MA006327A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Full Product Name
Mouse anti-Homo sapiens (Human) CYC1 Monoclonal antibody
Uniprot No.
Target Names
CYC1
Alternative Names
Cytochrome c1, heme protein, mitochondrial (EC:7.1.1.8); Complex III subunit 4; Complex III subunit IV; Cytochrome b-c1 complex subunit 4; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; Cytochrome c-1
Raised in
Mouse
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Cytochrome c1 protein (85-325AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
IgG1
Clone No.
27E12D11
Purification Method
>95%, Protein A purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, FC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
FC 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Mice were immunized with the recombinant human CYC1 protein (85-325aa), followed by the isolation of B cells from the mouse spleen, the fusion of these B cells with myeloma cells, and the selection of hybridomas that produce CYC1 antibodies. The CYC1 antibody-generating hybridomas were cultured to collect the CYC1 monoclonal antibodies. The CYC1 monoclonal antibody specifically reacts with human and mouse species and is suitable for ELISA, WB, and FC applications. The antibody is purified using protein A affinity chromatography and has a purity of over 95%.

CYC1 plays a crucial role in cellular energy metabolism and cell death regulation. It participates in the transfer of electrons between complexes III and IV of the respiratory chain, which drives the proton gradient across the mitochondrial inner membrane necessary for ATP synthesis. CYC1 also regulates apoptosis, or programmed cell death, by modulating the release of cytochrome c from mitochondria, which triggers the activation of caspases and leads to cell death.

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Target Background

Function
Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. Cytochrome c1 is a catalytic core subunit containing a c-type heme. It transfers electrons from the [2Fe-2S] iron-sulfur cluster of the Rieske protein to cytochrome c.
Gene References into Functions
  1. these results indicate that CYC1 plays important roles in cell proliferation and comedo necrosis through the elevated oxidative phosphorylation activity in human ductal carcinoma in situ PMID: 28394473
  2. results indicate that miR-661 plays a tumor suppressor role in OS mediated by the downregulation of CYC1, suggesting a potential mechanism underlying cell death resistance in Osteosarcoma. PMID: 28391262
  3. CYC1 promoted tumor metastasis via suppressing activation of AMPK and contributed to tumor growth via facilitating production of ATP. PMID: 27239088
  4. FASL, granzyme B, and cytochrome c blood expression reflects breast cancer progression and response to therapy. (Review) PMID: 27117663
  5. our results provide evidence that CYC1 plays an important role in OS tumorigenesis, and modulation of CYC1 may be an effective strategy to potentiate OS to apoptotic induction by TRAIL. PMID: 25562155
  6. Mutations in CYC1 cause insulin-responsive hyperglycemia. PMID: 23910460
  7. HeLa cells expressing non-cleavable cytochrome c1 maintain mitochondrial morphology and functions. PMID: 21577235
  8. Decreased electron Transport Complex IV activity is associated with ulcerative colitis. PMID: 20440543
  9. Addition of H202 to tendon fibroblasts in vitro resulted in oxidative stress-induced apoptosis and the release of CYC1. PMID: 12788227
  10. the Ca(2+) spike, mitochondrial Bcl-2 presensitization, and subsequent Delta psi(m) transition, Bax translocation, and ROS generation are important upstream signals for cytochrome c release upon stimulation by a Bcl-2 inhibitor. PMID: 14966123
  11. Sensitivity of Cyt c detection in -linked immunosorbent assay is reduced in the presence of serum leucine-rich alpha-2-glycoprotein-1. PMID: 16699948

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Involvement in disease
Mitochondrial complex III deficiency, nuclear 6 (MC3DN6)
Subcellular Location
Mitochondrion inner membrane; Single-pass membrane protein.
Protein Families
Cytochrome c family
Database Links

HGNC: 2579

OMIM: 123980

KEGG: hsa:1537

STRING: 9606.ENSP00000317159

UniGene: Hs.289271

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