Human cytochrome c-1 (CYC1) ELISA kit

Instructions
Code CSB-E17854h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cytochrome c-1
Alternative Names Complex III subunit 4 ELISA Kit; Complex III subunit IV ELISA Kit; CY1_HUMAN ELISA Kit; CYC1 ELISA Kit; Cytochrome b c1 complex subunit 4 ELISA Kit; Cytochrome b-c1 complex subunit 4 ELISA Kit; Cytochrome bc1 complex subunit 4 ELISA Kit; Cytochrome c 1 ELISA Kit; Cytochrome c-1 ELISA Kit; Cytochrome c1; heme protein; mitochondrial ELISA Kit; Ubiquinol cytochrome c reductase complex cytochrome c1 subunit ELISA Kit; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit ELISA Kit; UQCR4 ELISA Kit
Abbreviation CYC1
Uniprot No. P08574
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of Human CYC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %89
Range %85-93
1:2Average %94
Range %90-98
1:4Average %92
Range %88-96
1:8Average %97
Range %93-101
Recovery
The recovery of Human CYC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10297-106
EDTA plasma (n=4)8783-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.863 2.967 2.915 2.817
10002.526 2.425 2.476 2.378
5001.875 1.776 1.826 1.728
2501.065 1.043 1.054 0.956
1250.547 0.524 0.536 0.438
62.50.284 0.272 0.278 0.180
31.250.198 0.189 0.194 0.096
00.097 0.098 0.098
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.
Gene References into Functions
  1. these results indicate that CYC1 plays important roles in cell proliferation and comedo necrosis through the elevated oxidative phosphorylation activity in human ductal carcinoma in situ PMID: 28394473
  2. results indicate that miR-661 plays a tumor suppressor role in OS mediated by the downregulation of CYC1, suggesting a potential mechanism underlying cell death resistance in Osteosarcoma. PMID: 28391262
  3. CYC1 promoted tumor metastasis via suppressing activation of AMPK and contributed to tumor growth via facilitating production of ATP. PMID: 27239088
  4. FASL, granzyme B, and cytochrome c blood expression reflects breast cancer progression and response to therapy. (Review) PMID: 27117663
  5. our results provide evidence that CYC1 plays an important role in OS tumorigenesis, and modulation of CYC1 may be an effective strategy to potentiate OS to apoptotic induction by TRAIL. PMID: 25562155
  6. Mutations in CYC1 cause insulin-responsive hyperglycemia. PMID: 23910460
  7. HeLa cells expressing non-cleavable cytochrome c1 maintain mitochondrial morphology and functions. PMID: 21577235
  8. Decreased electron Transport Complex IV activity is associated with ulcerative colitis. PMID: 20440543
  9. Addition of H202 to tendon fibroblasts in vitro resulted in oxidative stress-induced apoptosis and the release of CYC1. PMID: 12788227
  10. the Ca(2+) spike, mitochondrial Bcl-2 presensitization, and subsequent Delta psi(m) transition, Bax translocation, and ROS generation are important upstream signals for cytochrome c release upon stimulation by a Bcl-2 inhibitor. PMID: 14966123
  11. Sensitivity of Cyt c detection in -linked immunosorbent assay is reduced in the presence of serum leucine-rich alpha-2-glycoprotein-1. PMID: 16699948

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Involvement in disease Mitochondrial complex III deficiency, nuclear 6 (MC3DN6)
Subcellular Location Mitochondrion inner membrane, Single-pass membrane protein, Intermembrane side
Protein Families Cytochrome c family
Database Links

HGNC: 2579

OMIM: 123980

KEGG: hsa:1537

STRING: 9606.ENSP00000317159

UniGene: Hs.289271

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