Human cytochrome c-1 (CYC1) ELISA kit

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Target Data

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Name: Human cytochrome c-1 (CYC1) ELISA kit
Brand: CUSABIO
SKU: CSB-E17854h

Product Details

Target Name

cytochrome c-1

Alternative Names

Complex III subunit 4 ELISA Kit; Complex III subunit IV ELISA Kit; CY1_HUMAN ELISA Kit; CYC1 ELISA Kit; Cytochrome b c1 complex subunit 4 ELISA Kit; Cytochrome b-c1 complex subunit 4 ELISA Kit; Cytochrome bc1 complex subunit 4 ELISA Kit; Cytochrome c 1 ELISA Kit; Cytochrome c-1 ELISA Kit; Cytochrome c1; heme protein; mitochondrial ELISA Kit; Ubiquinol cytochrome c reductase complex cytochrome c1 subunit ELISA Kit; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit ELISA Kit; UQCR4 ELISA Kit

Abbreviation

CYC1

Uniprot No.

P08574

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Human CYC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-93
1:2	Average %	94
	Range %	90-98
1:4	Average %	92
	Range %	88-96
1:8	Average %	97
	Range %	93-101

Recovery

The recovery of Human CYC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	97-106
EDTA plasma (n=4)	87	83-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.863	2.967	2.915	2.817
1000	2.526	2.425	2.476	2.378
500	1.875	1.776	1.826	1.728
250	1.065	1.043	1.054	0.956
125	0.547	0.524	0.536	0.438
62.5	0.284	0.272	0.278	0.180
31.25	0.198	0.189	0.194	0.096
0	0.097	0.098	0.098

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Target Data

Function	This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.
Gene References into Functions	these results indicate that CYC1 plays important roles in cell proliferation and comedo necrosis through the elevated oxidative phosphorylation activity in human ductal carcinoma in situ PMID: 28394473 results indicate that miR-661 plays a tumor suppressor role in OS mediated by the downregulation of CYC1, suggesting a potential mechanism underlying cell death resistance in Osteosarcoma. PMID: 28391262 CYC1 promoted tumor metastasis via suppressing activation of AMPK and contributed to tumor growth via facilitating production of ATP. PMID: 27239088 FASL, granzyme B, and cytochrome c blood expression reflects breast cancer progression and response to therapy. (Review) PMID: 27117663 our results provide evidence that CYC1 plays an important role in OS tumorigenesis, and modulation of CYC1 may be an effective strategy to potentiate OS to apoptotic induction by TRAIL. PMID: 25562155 Mutations in CYC1 cause insulin-responsive hyperglycemia. PMID: 23910460 HeLa cells expressing non-cleavable cytochrome c1 maintain mitochondrial morphology and functions. PMID: 21577235 Decreased electron Transport Complex IV activity is associated with ulcerative colitis. PMID: 20440543 Addition of H202 to tendon fibroblasts in vitro resulted in oxidative stress-induced apoptosis and the release of CYC1. PMID: 12788227 the Ca(2+) spike, mitochondrial Bcl-2 presensitization, and subsequent Delta psi(m) transition, Bax translocation, and ROS generation are important upstream signals for cytochrome c release upon stimulation by a Bcl-2 inhibitor. PMID: 14966123 Sensitivity of Cyt c detection in -linked immunosorbent assay is reduced in the presence of serum leucine-rich alpha-2-glycoprotein-1. PMID: 16699948 Show More Hide All
Involvement in disease	Mitochondrial complex III deficiency, nuclear 6 (MC3DN6)
Subcellular Location	Mitochondrion inner membrane, Single-pass membrane protein, Intermembrane side
Protein Families	Cytochrome c family
Database Links	HGNC: 2579 OMIM: 123980 KEGG: hsa:1537 STRING: 9606.ENSP00000317159 UniGene: Hs.289271

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Check Code：

Code	CSB-E17854h
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.