USP6 Monoclonal Antibody

Code CSB-MA025747A0m
Size US$350 How to order?
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  • Western Blot
    Positive WB detected in: USP6 antibody at 1:1000
    Lane 1: MCF-7 whole cell lysate
    Lane 2: THP-1 whole cell lysate
    Lane 3: SH-SY5Y whole cell lysate
    Lane 4: Jurkat whole cell lysate
    Lane 5: HEK293 whole cell lysate
    Lane 6: Hela whole cell lysate
    Secondary
    Goat polyclonal to Mouse IgG at 1/20000 dilution
    Predicted band size: 89, 122, 159 KDa
    Observed band size: 89, 122, 159 KDa
    Exposure time: 1min
  • Immunofluorescence staining of HeLa cells with CSB-MA025747A0m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
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Product Details

Full Product Name Human anti-Homo sapiens (Human) USP6 Monoclonal antibody
Uniprot No. P35125
Target Names USP6
Alternative Names Deubiquitinating enzyme 6 antibody; HRP1 antibody; Proto-oncogene TRE-2 antibody; TRE17 antibody; TRE2 antibody; Ubiquitin carboxyl-terminal hydrolase 6 antibody; Ubiquitin specific protease 6 antibody; Ubiquitin thiolesterase 6 antibody; Ubiquitin-specific-processing protease 6 antibody; UBP6_HUMAN antibody; USP6 antibody
Raised in Human
Species Reactivity Human
Immunogen Recombinant Human Ubiquitin carboxyl-terminal hydrolase 6 protein (348-535AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG1
Clone No. 3C3G2
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.
Gene References into Functions
  1. None of the genitourinary pseudosarcomatous myofibroblastic proliferations was found to harbour USP6 (0/12), ROS1 (0/8) or ETV6 (0/7) rearrangements PMID: 29617048
  2. we identified seven novel fusion partners for USP6 in nodular fasciitis, highlighting the importance of USP6 expression and promoter-swapping fusions in the etiology of this neoplasm PMID: 28752842
  3. Report the presence of USP6 rearrangements in a subset of cellular fibroma of tendon sheath. PMID: 27125357
  4. our studies highlight Jak1 as the first identified substrate for USP6, and they offer a mechanistic rationale for the clinical investigation of Jak and STAT3 inhibitors as therapeutics for the treatment of bone and soft tissue tumors along with other neoplasms driven by USP6 overexpression PMID: 27440725
  5. Molecular analyses revealed the presence and amplification of the novel PPPR6-USP6 gene fusion, which resulted in USP6 mRNA transcriptional upregulation. These findings further support the oncogenic role of the USP6 protease in mesenchymal neoplasia and expand the biologic potential of Nodular fasciitis PMID: 27113271
  6. It was shown that TRE17 activates the classical NF-kappa B pathway through an atypical mechanism that does not involve IkappaB degradation. Optimal activation of NF-kappa B by TRE17 required both catalytic subunits of IkappaB kinase. PMID: 22081069
  7. USP6 fluorescence in-situ hybridization is a useful ancillary test in cases where nodular fasciitis is a potential diagnostic consideration. PMID: 27271298
  8. the deubiquitylase ubiquitin-specific protease 6 (USP6) as a potent activator of Wnt signaling. USP6 enhances Wnt signaling by deubiquitylating Fzds, thereby increasing their cell-surface abundance. PMID: 27162353
  9. TRE17/USP6 regulates ubiquitylation and trafficking of cargo proteins that enter cells by clathrin-independent endocytosis PMID: 25179595
  10. 8 of the 9 giant cell reparative granulomas from hands and feet showed rearrangements of the USP6 gene compared with none of 8 gnathic lesions PMID: 24742829
  11. we discuss the clinicopathologic features, molecular pathology, and pathogenesis of ABC and nodular fasciitis in relation to USP6 PMID: 23769422
  12. identification of a USP6 gene rearrangement is helpful in making a diagnosis of nodular fasciitis. PMID: 23748914
  13. manipulating USP6 expression levels alters the ability of cells to migrate and to divide. Cell proliferation and progression through cytokinesis depend on USP6 expression PMID: 22188517
  14. TRE17 is sufficient to initiate tumorigenesis, identify MMPs as novel TRE17 effectors that likely contribute to aneurysmal bone cyst pathogenesis. PMID: 20418905
  15. TRE17 coprecipitated specifically with the active forms of Cdc42 and Rac1 in vivo. TRE17 is part of a novel effector complex for Cdc42 and Rac1, potentially contributing to their effects on actin remodeling. PMID: 12612085
  16. Complementation tests in yeasts indicate that Tre2 codes for a nonfunctional RabGAP. PMID: 14521938
  17. Deregulated USP6 transcription is associated with aneurysmal bone cyst PMID: 15026324
  18. primary aneurysmal bone cysts are mesenchymal neoplasms exhibiting USP6 and/or CDH11 oncogenic rearrangements PMID: 15509545
  19. TRE17 associates directly with Arf6 in its GDP- but not GTP-bound state. PMID: 15509780
  20. Tre2 oncogene seems to encode a nonfunctional Rab GAP. As regions flanking the TBC domain may be crucial for catalytic activity PMID: 16099424
  21. Ca2+/CaM has a role in regulating ubiquitination through direct interaction with TRE17 PMID: 16127172
  22. The lack of secondary structure of the region flanking the TBC domain in TRE2 may explain why this region plays a role in the lack of GAP activity, even when a potentially functional TBC domain is present. PMID: 17701273
  23. No USP6 rearrangements were found in cherubism or brown tumors. USP6 rearrangements were identified in 2 patients with myositis ossificans. PMID: 18265974

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Involvement in disease A chromosomal aberration involving USP6 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with CDH11. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation (PubMed:15026324).
Subcellular Location Cell membrane. Cytoplasm. Endosome. Note=Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton.
Protein Families Peptidase C19 family
Tissue Specificity Testis specific. Expressed in various cancer cell lines.
Database Links

HGNC: 12629

OMIM: 604334

KEGG: hsa:9098

STRING: 9606.ENSP00000250066

UniGene: Hs.448851

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