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| Code | CSB-MP006595HU |
| Size | $428 |
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| Description |
This Human DDR1 recombinant protein was produced in Mammalian cell, where the gene sequence encoding Human DDR1 (21-417aa) was expressed with the C-terminal 10xHis tag. The purity of this DDR1 protein was greater than 95% by SDS-PAGE. The activity was validated by its binding ability in a functional ELISA. DDR1 (Epithelial discoidin domain-containing receptor 1), also known as CD167a, MCK-10, CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE, and HGK2, is present in many types of epithelial cells. It is expressed at low levels in most adult tissues, with the highest expression in brain, lung, placenta, and kidney, and is highly expressed in a variety of cancer cells. DDR1 protein is mainly localized in the cell membrane and extracellular secretion region. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Endotoxin | Less than 1.0 EU/ug as determined by LAL method. |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Human DDR1 at 2 μg/mL can bind Anti-DDR1 recombinant antibody (CSB-RA006595MA1HU), the EC50 is 2.824-3.810 ng/mL. |
| Target Names | DDR1 |
| Uniprot No. | Q08345 |
| Alternative Names |
(Epithelial discoidin domain receptor 1)(CD167 antigen-like family member A)(Cell adhesion kinase)(Discoidin receptor tyrosine kinase)(HGK2)(Mammary carcinoma kinase 10)(MCK-10)(Protein-tyrosine kinase 3A)(Protein-tyrosine kinase RTK-6)(TRK E)(Tyrosine kinase DDR)(Tyrosine-protein kinase CAK)(CD antigen CD167a)
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| Molecular Characterization |  |
| Species | Homo sapiens (Human) |
| Source | Mammalian cell |
| Expression Region | 21-417aa |
| Target Protein Sequence | DMKGHFDPAKCRYALGMQDRTIPDSDISASSSWSDSTAARHSRLESSDGDGAWCPAGSVFPKEEEYLQVDLQRLHLVALVGTQGRHAGGLGKEFSRSYRLRYSRDGRRWMGWKDRWGQEVISGNEDPEGVVLKDLGPPMVARLVRFYPRADRVMSVCLRVELYGCLWRDGLLSYTAPVGQTMYLSEAVYLNDSTYDGHTVGGLQYGGLGQLADGVVGLDDFRKSQELRVWPGYDYVGWSNHSFSSGYVEMEFEFDRLRAFQAMQVHCNNMHTLGARLPGGVECRFRRGPAMAWEGEPMRHNLGGNLGDPRARAVSVPLGGRVARFLQCRFLFAGPWLLFSEISFISDVVNNSSPALGGTFPPAPWWPPGPPPTNFSSLELEPRGQQPVAKAEGSPTA |
| Mol. Weight | 46.8 kDa |
| Protein Length | Partial |
| Tag Info |
C-terminal 10xHis-tagged |
| Form |
Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing. Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
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| Gene References into Functions |
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| Subcellular Location | [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted.; [Isoform 4]: Cell membrane; Single-pass type I membrane protein. |
| Protein Families | Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily |
| Tissue Specificity | Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the b |
| Database Links |
HGNC: 2730 OMIM: 600408 KEGG: hsa:780 STRING: 9606.ENSP00000365759 UniGene: Hs.631988 |
