Recombinant Human Epithelial discoidin domain-containing receptor 1(DDR1),partial (Active)

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Code CSB-MP006595HU
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized Human DDR1 at 2 μg/ml can bind Anti-DDR1 recombinant antibody (CSB-RA006595MA1HU), the EC50 is 2.824-3.810 ng/mL. Biological Activity Assay
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Product Details

Description

This Human DDR1 recombinant protein was produced in Mammalian cell, where the gene sequence encoding Human DDR1 (21-417aa) was expressed with the C-terminal 10xHis tag. The purity of this DDR1 protein was greater than 95% by SDS-PAGE. The activity was validated by its binding ability in a functional ELISA.

DDR1 (Epithelial discoidin domain-containing receptor 1), also known as CD167a, MCK-10, CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE, and HGK2, is present in many types of epithelial cells. It is expressed at low levels in most adult tissues, with the highest expression in brain, lung, placenta, and kidney, and is highly expressed in a variety of cancer cells. DDR1 protein is mainly localized in the cell membrane and extracellular secretion region.
DDR1 is a member of the receptor tyrosine kinase (RTK) family, a receptor tyrosine kinase activated by matrix collagen, which is abnormally up-regulated in a variety of tumors. It is associated with a poor prognosis, so it has received extensive attention in the pharmaceutical field. Current studies have shown that, in addition to being involved in key cellular processes such as cell proliferation, migration, survival, and differentiation, DDR1 also controls extracellular matrix (ECM) remodeling. Inhibition of DDR1 expression can reduce immunosuppressive effects in the tumor microenvironment. Therefore, DDR1 is considered as a potential target for tumor immunotherapy.

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/ug as determined by LAL method.
Activity Measured by its binding ability in a functional ELISA. Immobilized Human DDR1 at 2 μg/mL can bind Anti-DDR1 recombinant antibody (CSB-RA006595MA1HU), the EC50 is 2.824-3.810 ng/mL.
Target Names DDR1
Uniprot No. Q08345
Alternative Names (Epithelial discoidin domain receptor 1)(CD167 antigen-like family member A)(Cell adhesion kinase)(Discoidin receptor tyrosine kinase)(HGK2)(Mammary carcinoma kinase 10)(MCK-10)(Protein-tyrosine kinase 3A)(Protein-tyrosine kinase RTK-6)(TRK E)(Tyrosine kinase DDR)(Tyrosine-protein kinase CAK)(CD antigen CD167a)
Molecular Characterization
Species Homo sapiens (Human)
Source Mammalian cell
Expression Region 21-417aa
Target Protein Sequence DMKGHFDPAKCRYALGMQDRTIPDSDISASSSWSDSTAARHSRLESSDGDGAWCPAGSVFPKEEEYLQVDLQRLHLVALVGTQGRHAGGLGKEFSRSYRLRYSRDGRRWMGWKDRWGQEVISGNEDPEGVVLKDLGPPMVARLVRFYPRADRVMSVCLRVELYGCLWRDGLLSYTAPVGQTMYLSEAVYLNDSTYDGHTVGGLQYGGLGQLADGVVGLDDFRKSQELRVWPGYDYVGWSNHSFSSGYVEMEFEFDRLRAFQAMQVHCNNMHTLGARLPGGVECRFRRGPAMAWEGEPMRHNLGGNLGDPRARAVSVPLGGRVARFLQCRFLFAGPWLLFSEISFISDVVNNSSPALGGTFPPAPWWPPGPPPTNFSSLELEPRGQQPVAKAEGSPTA
Mol. Weight 46.8 kDa
Protein Length Partial
Tag Info C-terminal 10xHis-tagged
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing. Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
Gene References into Functions
  1. Data (including data from studies using knockout mice) suggest that DDR1 plays role in promoting mammary tumor growth; tumor-extrinsic DDR1 appears to be required for promotion of mammary tumors by interleukin-6. PMID: 29298894
  2. our study provides a novel regulatory pathway involving TM4SF1, DDR1, MMP2 and MMP9, which promotes the formation and function of invadopodia to support cell migration and invasion in pancreatic cancer. PMID: 28368050
  3. The three well-conserved seed matched sites for miR-199a/b-5p in the discoidin domain receptor 1 (DDR1) 3'-UTR were targeted, and miRNA binding to at least two sites was required for DDR1 inhibition. PMID: 29429150
  4. E2F1 knockdown decreased the expression of discoidin domain receptor 1 (DDR1) which plays a crucial role in many fundamental processes such as cell differentiation, adhesion, migration and invasion. PMID: 29039472
  5. Furthermore, the inhibition of DDR1 with 7rh showed striking efficacy in combination with chemotherapy in orthotopic xenografts and autochthonous pancreatic tumors where it significantly reduced DDR1 activation and downstream signaling, reduced primary tumor burden, and improved chemoresponse. PMID: 28864681
  6. These findings demonstrate a critical role of miR-199a-3p/DDR1 pathway in ovarian cancer development. PMID: 28743276
  7. findings demonstrate that, in human breast cancer cells, DDR1 regulates IR expression and ligand dependent biological actions. This novel functional crosstalk is likely clinically relevant PMID: 28591735
  8. These results support an activation mechanism of DDR1 whereby collagen induces lateral association of DDR1 dimers and phosphorylation between dimers. PMID: 28590245
  9. Data suggest that IGF-I/IGF-IR system triggers stimulatory actions through both GPER and DDR1 in aggressive tumors as mesothelioma and lung tumors. PMID: 27384677
  10. we further analyzed the CpG methylation levels at the DDR1 promoter in EOC cells and found that the CpG methylation levels of DDR1 promoter correlated negatively with the expression of DDR1 along the EMT spectrum. Therefore, EMT stratification could be a potential biomarker to predict patient response to DDR1-targeting drugs. PMID: 28887161
  11. This study suggested that DDR1 and DDR2 knockdown alters brain immunity and significantly reduces the level of triggering receptor expressed on myeloid cells (TREM)-2 and microglia. PMID: 28863860
  12. Isoform b of DDR1 is responsible for collagen I-induced up-regulation of N-cadherin and tyrosine 513 of DDR1b is necessary. PMID: 27605668
  13. Reduced DDR1 expression may be implicated in impaired melanocyte adhesion process involved in vitiligo pathogenesis PMID: 26091274
  14. data demonstrate that TGF-beta1 favors linear invadosome formation through the expressions of both the inducers, such as collagen and LOXL2, and the components such as DDR1 and MT1-MMP of linear invadosomes in cancer cells. Meanwhile, our data uncover a new TGF-beta1-dependent regulation of DDR1 expression. PMID: 27720259
  15. DDR1 overexpression promoted GC cell proliferation (p < 0.05), migration (p < 0.01), and invasion (p < 0.01), and accelerated the growth (p < 0.05) as well as the microvessel formation (p < 0.01) of transplantation tumor in nude mice. PMID: 27179963
  16. Our results suggest that DDR1 is both a prognostic marker for renal clear cell carcinoma and a potential functional target for therapy PMID: 27020590
  17. Study concludes that non-canonical DDR1 signaling enables breast cancer cells to exploit the ubiquitous interstitial matrix component collagen I to undergo metastatic reactivation in multiple target organs. PMID: 27368100
  18. Upregulation of DDR1 collagen receptor is associated with breast cancer. PMID: 26655502
  19. Knockdown DDR1 reversed the effects of Galpha13 knockdown on cell-cell adhesion and proteolytic invasion in three-dimensional collagen. PMID: 26589794
  20. Data suggest SCl2 (Streptococcus pyogenes) binds to DDR (DDR1, DDR2) ectodomain w/o stimulating receptor signaling; here, protein engineering was used to construct SCl-like proteins that inhibit collagen-DDR interactions and macrophage migration. PMID: 26702058
  21. DDR1 is a key modulator of RIT activity. PMID: 26719540
  22. DDR1 expression is a prognostic indicator in pancreatic ductal carcinoma. PMID: 26297342
  23. alpha5(IV), but not alpha1(IV), promotes lung cancer cell proliferation and tumor angiogenesis through non-integrin collagen receptor DDR1-mediated ERK activation. PMID: 25992553
  24. Hypoxia can increase DDR1 expression in pituitary adenoma cells, leading to improved MMP-2 and MMP-9 secretion, and promoting pituitary adenoma cell proliferation and invasion. PMID: 26286316
  25. suggest that DDR1 suppression may enhance adipose-derived stem cell chondrogenesis by enhancing the expression of chondrogenic genes and cartilaginous matrix deposition. PMID: 25673773
  26. MT1-MMP-discoidin domain receptor 1 axis regulates collagen-induced apoptosis in breast cancer cells PMID: 25774665
  27. Defective Ca(2+) binding in a conserved binding site causes incomplete N-glycan processing and endoplasmic reticulum trapping of DDR1 and DDR2 receptors. PMID: 25470979
  28. A DDR1(Low)/DDR2(High) protein profile is associated with TNBC and may identify invasive carcinomas with worse prognosis. PMID: 25667101
  29. We uncovered ten new mutations in TNK2 and DDR1 within serous and endometrioid ECs, thus providing novel insights into the mutation spectrum of each gene in EC. PMID: 25427824
  30. Silencing of DDR1 inhibited tumor cell growth and motility, and induced TGFBI expression, both in vitro and in vivo. PMID: 25369402
  31. found an inverse correlation between ZEB1 and DDR1 expression in various cancer cell lines and in human breast carcinoma tissues PMID: 25155634
  32. DDR1 depletion blocked cell invasion in a collagen gel PMID: 25422375
  33. The expression of the DDR1 protein significantly correlated with poor disease-free survival in patients with serous ovarian cancer. PMID: 21541037
  34. High DDR1 protein expression is associated with recurrence in ameloblastomas. PMID: 24723326
  35. In this review we highlight the mechanisms whereby DDRs(DDR1 and DDR2) regulate two important processes, namely inflammation and tissue fibrosis. PMID: 24361528
  36. Report aberrant methylation of DDR1 in men with nonobstructive azoospermia. PMID: 25064398
  37. Crystal structures of DDR1 reveal a DFG-out conformation (DFG, Asp-Phe-Gly) of the kinase domain that is stabilized by a salt bridge between the activation loop and alphaD helix. Differences to Abelson kinase are observed in the DDR1 P-loop's beta-hairpin. PMID: 24768818
  38. These findings indicate that the extracellular juxtamembrane region of DDR1 is exceptionally flexible and does not constrain the basal or ligand-activated state of the receptor. PMID: 24671415
  39. N-glycosylation at the highly conserved (211)NDS motif evolved to act as a negative repressor of DDR1 phosphorylation in the absence of ligand PMID: 24509848
  40. regulation of DDRs by glucose PMID: 24018687
  41. Upregulation of DDR1 induced by collagen I may contribute to the development and progression of NSCLC. PMID: 23761027
  42. The DDR1 extracellular domain plays a crucial role in receptor oligomerization which mediates high-affinity interactions with its ligand. PMID: 23810922
  43. We show that expression of the latent LMP1 in primary human germinal center B cells, the presumed progenitors of HRS cells, upregulates discoidin domain receptor 1 (DDR1), a receptor tyrosine kinase activated by collagen. PMID: 24136166
  44. these data suggest that NEP can augment taxane-induced apoptosis through inhibition of Akt/Bad signaling PMID: 22895534
  45. the expression of the novel collagen receptor discoidin domain receptor 1 (DDR1) by human MKs at both mRNA and protein levels and provide evidence of DDR1 involvement in the regulation of MK motility on type I collagen PMID: 23530036
  46. DDR1 expression was not predictive for patient survival in human breast cancer PMID: 23307244
  47. DD1 mRNA and protein levels were higher in patients with recurrent Hepatocellular carcinoma , suggesting this gene maybe involved in tumor invasion and metastasis. PMID: 22752569
  48. a role for the collagenase of membrane-type MMPs in regulation of DDR1 cleavage and activation at the cell-matrix interface. PMID: 23519472
  49. Discoidin domain receptors promote alpha1beta1- and alpha2beta1-integrin mediated cell adhesion to collagen by enhancing integrin activation. PMID: 23284937
  50. Discoidin domain receptors are unique receptor tyrosine kinases in collagen-mediated signaling [review] PMID: 23335507

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Subcellular Location [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted.; [Isoform 4]: Cell membrane; Single-pass type I membrane protein.
Protein Families Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
Tissue Specificity Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the b
Database Links

HGNC: 2730

OMIM: 600408

KEGG: hsa:780

STRING: 9606.ENSP00000365759

UniGene: Hs.631988

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