Greater than 95% as determined by SDS-PAGE.
Less than 1.0 EU/ug as determined by LAL method.
①Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D at 2 μg/mL can bind biotinylated Pepinemab, the EC50 is 1.005-1.776 ng/mL. ②Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D at 5 μg/mL can bind human PLXNB1 (CSB-MP018222HU2k6), the EC50 is 0.8179-1.357 μg/mL.
Homo sapiens (Human)
Target Protein Sequence
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
The C-terminally hFc-tagged human semaphorin-4D (SEMA4D) is an active recombinant protein expressed in mammalian cells. Its expression region corresponds to amino acids 22-734 of the human SEMA4D. The purity of this SEMA4D protein is greater than 95% measured by SDS-PAGE. It migrated to the molecular weight band of about 120 kDa on the gel. Its endotoxin is less than 1.0 EU/ug determined by the LAL method. In a functional ELISA, this recombinant SEMA4D protein can bind biotinylated Pepinemab. The EC50 is 1.005-1.776 ng/mL. SEMA4D, also known as CD100, is involved in immune regulation, bone formation, platelet inactivation, angiogenesis stimulation, and cancer bone metastasis.