SEMA4D (Semaphorin 4D) is a transmembrane protein that consists of an extracellular domain, a transmembrane domain, and a cytoplasmic domain. Its extracellular domain contains Semaphorin domains, which are used to interact with other proteins and trigger signal transduction. SEMA4D plays a crucial role in neural development, being involved in guiding neuronal axons and forming synapses. Through interactions with its receptors, such as the Plexin family receptors, SEMA4D can influence neuronal growth and connectivity. SEMA4D also has a significant role in the immune system. It can modulate the activation and migration of immune cells. Interactions between SEMA4D and its receptors, such as CD72 and Plexin B2, can affect the functions of T cells, B cells, and macrophages. SEMA4D is a multifunctional molecule that operates in neural development, the immune system, and diseases. Studying the functions and regulatory mechanisms of SEMA4D helps us better understand the regulation of neural development and immune responses and contributes to the development of new therapeutic approaches, especially in the field of diseases like cancer.
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Popular Product
Protein
● Recombinant Human Semaphorin-4D(SEMA4D),partial (Active) CSB-MP835707HU
Validated Data
SDS
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
ELISA
Activity: Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D at 2 μg/ml can bind Pepinemab, the EC50 is 4.035-5.743 ng/mL.
● Recombinant Mouse Semaphorin-4D(Sema4d),partial (Active) CSB-MP020990MO
Validated Data
SDS
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
ELISA
Activity: Measured by its binding ability in a functional ELISA. Immobilized Pepinemab at 2 μg/ml can bind Mouse Sema4d, the EC50 is 4.878-7.446 ng/ml.
● Recombinant Human Semaphorin-4D(SEMA4D),partial (Active) CSB-MP835707HUd9
Validated Data
SDS
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
ELISA
Activity: Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D at 2 μg/ml can bind biotinylated Pepinemab, the EC50 is 1.005-1.776 ng/mL.
ELISA
Activity: Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D at 5 μg/ml can bind human PLXNB1 (CSB-MP018222HU2k6), the EC50 is 0.8179-1.357 μg/mL.
● Recombinant Macaca mulatta Semaphorin-4D isoform 1(SEMA4D),partial (Active) CSB-MP3947MOW
Validated Data
SDS
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
ELISA
Activity: Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque SEMA4D at 2 μg/mL can bind Anti-SEMA4D recombinant antibody (CSB-RA835707A1HU), the EC50 is 1.943-2.724 ng/mL.
Antibody
● SEMA4D Recombinant Monoclonal Antibody CSB-RA835707A2HU
Validated Data
ELISA
The Binding Activity of Mouse Sema4d with Anti-SEMA4D recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Anti-SEMA4D recombinant antibody at 2 μg/mL can bind Mouse Sema4d (CSB-MP020990MO), the EC50 is 5.829-16.86 ng/mL.
ELISA
The Binding Activity of Human SEMA4D with Anti-SEMA4D recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human SEMA4D (CSB-MP835707HU) at 2 μg/mL can bind Anti- SEMA4D recombinant antibody, the EC50 is 60.38-152.6 ng/mL.
FC
Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
● SEMA4D Recombinant Monoclonal Antibody CSB-RA835707A1HU
Validated Data
FC
Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2µg/1*106 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
ELISA
The Binding Activity of Mouse Sema4d with Anti-SEMA4D recombinant antibody.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Sema4d (CSB-MP020990MO) at 2 μg/mL can bind Anti-SEMA4D recombinant antibody, the EC50 is 98.14-174.8 ng/mL.
ELISA
The Binding Activity of Human SEMA4D with Anti-SEMA4D recombinant antibody.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human SEMA4D (CSB-MP835707HU) at 2 μg/mL can bind Anti-SEMA4D recombinant antibody, the EC50is 1.082-4.805 ng/mL.
Stable Cell Lines
● HEK293T/Human SEMA4D Stable Cell Line CSB-SC835707HU
