CHD1 Antibody

Datasheet
Code CSB-PA005325ESR2HU
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Uniprot No. O14646
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  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA005325ESR2HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA005325ESR2HU at dilution of 1:100

  • Chromatin Immunoprecipitation Hela (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-CDH1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers (CSB-PP005325HU) against the RPL30 promoter.

Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Immunogen Recombinant Human Chromodomain-helicase-DNA-binding protein 1 protein (1501-1710AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC, ChIP; Recommended dilution: IHC:1:20-1:200
Relevance ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells.
Form Liquid
Conjugate Non-conjugated
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Chromodomain-helicase-DNA-binding protein 1 (CHD-1) (EC 3.6.4.12) (ATP-dependent helicase CHD1), CHD1
Immunogen Species Human
Research Area Epigenetics and Nuclear Signaling
Target Names CHD1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells.
Subcellular Location Nucleus, Cytoplasm
Protein Families SNF2/RAD54 helicase family
Tissue Specificity Expressed in many tissues including in the brain, where the highest level of expression is found in the cerebellum and basal ganglia.
Database Links

HGNC: 1915

OMIM: 602118

KEGG: hsa:1105

STRING: 9606.ENSP00000284049

UniGene: Hs.643465

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