Di-methyl-H3F3A (K79) Antibody

Datasheet
Code CSB-PA010109OA79me2HU
Size US$319
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate
    All lanes: H3F3A antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H3F3A (CSB-PA010109OA79me2HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Product Details

Full Product Name Rabbit anti-Human H3F3A Polyclonal antibody
Uniprot No. P84243
Target Names H3F3A
Alternative Names Histone H3.3, H3F3A; H3F3B, H3.3A H3F3; H3.3B
Raised in Rabbit
Species Reactivity Human
Immunogen Peptide sequence around site of Di-methyl-Lys (79) derived from Human Histone H3.3
Immunogen Species Human
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, ChIP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Involvement in disease Glioma (GLM)
Subcellular Location Nucleus, Chromosome
Protein Families Histone H3 family
Database Links

HGNC: 4764

OMIM: 137800

KEGG: hsa:3020

STRING: 9606.ENSP00000355778

UniGene: Hs.180877

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