H3F3A (Ab-79) Antibody

Datasheet

Code CSB-PA010109OA79nme2HU
Size US$319
Uniprot No. P84243
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Rat brain tissue, Rat spleen tissue
    All lanes: H3F3A antibody at 0.73μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5μg anti-H3F3A (CSB-PA0101090A79nme2HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Immunogen Peptide sequence around site of Di-methyl-Lys (79) derived from Human Histone H3.3
Raised in Rabbit
Species Reactivity Human, Rat
Tested Applications ELISA, WB, ChIP; Recommended dilution: WB:1:200-1:2000
Relevance Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Histone H3.3, H3F3A; H3F3B, H3.3A H3F3; H3.3B
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Research Area Epigenetics and Nuclear Signaling
Target Names H3F3A
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Still Have Questions? Leave a Message or Start an on-line Chat
Function Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Involvement in disease Glioma (GLM)
Subcellular Location Nucleus, Chromosome
Protein Families Histone H3 family
Database Links

HGNC: 4764

OMIM: 137800

KEGG: hsa:3020

STRING: 9606.ENSP00000355778

UniGene: Hs.180877

Related Products

H3F3A Antibodies

H3F3A Antibodies for Human

H3F3A Antibodies for Homo sapiens (Human)

H3F3A Antibodies for Danio rerio (Zebrafish) (Brachydanio rerio)

H3F3A Proteins

H3F3A Proteins for Mus musculus (Mouse)

H3F3A Proteins for Oryctolagus cuniculus (Rabbit)

H3F3A Proteins for Homo sapiens (Human)

H3F3A Proteins for Danio rerio (Zebrafish) (Brachydanio rerio)

H3F3A Proteins for Sus scrofa (Pig)

H3F3A cDNA

H3F3A cDNA for Sus scrofa (Pig)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.