HDAC1 Antibody

Code CSB-PA17869A0Rb
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA17869A0Rb at dilution of 1: 100

  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA17869A0Rb at dilution of 1: 100

  • Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA17869A0Rb at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA17869A0Rb at dilution of 1:100

  • Immunofluorescence staining of Hela cells with CSB-PA17869A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Chromatin Immunoprecipitation MCF-7 (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-HDAC1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers (CSB-PP17869HU) against the P21 promoter.

The Latest Promotion Free Antibody trial simple
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) HDAC1 Polyclonal antibody
Uniprot No. Q13547
Target Names HDAC1
Alternative Names DKFZp686H12203 antibody; GON 10 antibody; HD1 antibody; HDAC 1 antibody; HDAC1 antibody; HDAC1_HUMAN antibody; Histone deacetylase 1 antibody; Reduced potassium dependency yeast homolog like 1 antibody; RPD3 antibody; RPD3L1 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Histone deacetylase 1 protein (1-482AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, IHC, IF, ChIP
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:100-1:500
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator
Gene References into Functions
  1. HDAC1,2 inhibitor either alone or when combined with doxorubicin decreases leukemia burden. PMID: 28579617
  2. miR-34a exhibited suppressive effects on ovarian cancer (OC) cells via directly binding and downregulating HDAC1 expression, which subsequently decreased the resistance to cisplatin and suppressed proliferation in OC cells. PMID: 29561664
  3. Study identified HDAC1, a key regulator of eukaryotic gene expression and many important cellular events, including cell proliferation, differentiation, cancer and immunity, as an interacting partner of ABIN1. PMID: 29058807
  4. r meta-analysis demonstrated an association between increased HDAC1 expression and better OS in Asian breast cancer patients PMID: 29738697
  5. Daxx directly binds to the DNA-binding domain of Slug, impeding histone deacetylase 1 (HDAC1) recruitment and antagonizing Slug E-box binding. This, in turn, stimulates E-cadherin and occludin expression and suppresses Slug-mediated epithelial-mesenchymal transition (EMT) and cell invasiveness. PMID: 28004751
  6. the knockdown of HDAC1/2 upregulated KLF5 protein but not KLF5 mRNA, and the increase in KLF5 protein level by silencing HDAC1/2 was at least in part due to decreased proteasomal degradation. PMID: 29679567
  7. HDAC1 knockdown by siRNA suppressed cell proliferation, and increased apoptosis and chemosensitivity by downregulating c-Myc and upregulating miR-34a. PMID: 30071534
  8. The HDAC1 expression was associated with the SNAIL expression in clinical samples. PMID: 29917299
  9. data show that Hdac1 and Hdac2 impact on Emu-myc B cell proliferation and apoptosis and suggest that a critical level of Hdac activity may be required for Emu-myc tumorigenesis and proper B cell development PMID: 27886239
  10. Data show there is allosteric communication between the inositol-binding site and the active sites in histone deacetylases HDAC1 and HDAC3. PMID: 27109927
  11. High HDAC1 expression is associated with gastrointestinal malignancy. PMID: 28424407
  12. Results show that proteasomal degradation of HDAC1 and HDAC3 by Vpr counteracts HIV-1 latency to reactivate the viral promoter. PMID: 27550312
  13. concomitant LSD1 and HDAC inhibition synergistically induces cell death in rhabdomyosarcoma cells by shifting the ratio of pro- and antiapoptotic BCL-2 proteins in favor of apoptosis, thereby engaging the intrinsic apoptotic pathway PMID: 28617441
  14. Hdac1 levels are increased in blood samples from patients with schizophrenia who had encountered early life stress (ELS), compared with patients without ELS experience. PMID: 28533418
  15. HDAC1 and HDAC2 suppress the expression of PPP2R3A/PR130, a regulatory subunit of the trimeric serine/threonine phosphatase 2 (PP2A). PMID: 29472538
  16. HDAC1 may therefore be considered an unfavorable progression indicator for glioma patients, and may also serve as a potential therapeutic target. PMID: 28624794
  17. new basis of DDX23-Linc00630-HDAC1 signal axis for understanding its pathogenicity, which could be further developed as a valuable therapeutic strategy PMID: 28473661
  18. Results show that histone deacetylase 1 (HDAC1) expression was positively correlated with YY1 transcription factor (YY1) in hepatocellular carcinoma (HCC) cell lines and primary tumor tissues. PMID: 28489564
  19. the combination of p63-positive and HDAC1-negative expressions can be a potential new way for distinguishing epidermal stem cells. PMID: 28672879
  20. Nuclear HDAC2 immunopositivity was significantly higher in actinic cheilitis (AC) when compared with lip squamous cell carcinoma (LSCC). HDAC1 and HAT1 nuclear immunostaining were higher in AC, with no statistical significance. When comparing data with our previous study, we found a positive correlation between HDAC1 X DNMT1/DNMT3b, HDAC2 X DNMT3b, and HAT1 X DNMT1/DNMT3b for certain studied groups. PMID: 28107582
  21. data reveal the mechanism by which chromatin remodeling and target gene expression are regulated by Ikaros alone and in complex with HDAC1 in B-ALL PMID: 26639180
  22. Histone deacetylases 1 and 2 cooperate in regulating BRCA1, CHK1, and RAD51 expression in acute myeloid leukemia cells. PMID: 28030834
  23. our findings identify a key role for c-Myc in TRAIL deregulation and as a biomarker of the anticancer action of HDACi in acute myeloid leukemia . PMID: 27358484
  24. The authors found that 2-aminoacetophenone regulates histone deacetylase 1 expression and activity, resulting in hypo-acetylation of lysine 18 of histone H3 at pro-inflammatory cytokine loci. Specifically, 2-aminoacetophenone induced reprogramming of immune cells occurs via alterations in histone acetylation of immune cytokines in vivo and in vitro. PMID: 27694949
  25. Histone deacetylases (HDACs) inhibitor MGCD0103 (MGCD) induces apoptosis by up-regulating p53 in CNE2 nasopharyngeal carcinoma (NPC) cells. PMID: 27283770
  26. Our findings suggest that up-regulation of UVRAG by HDAC1 inhibition potentiates DNA-damage-mediated cell death in colorectal cancer cells PMID: 29277783
  27. The findings suggest that OGT promotes the O-GlcNAc modification of HDAC1 in the development of progression hepatocellular carcinoma. PMID: 27060025
  28. HDAC1 promoted migration and invasion of gallbladder tumor cells by binding with TCF12 to promote epithelial mesenchymal transformation. PMID: 27092878
  29. HDAC1 depletion-induced p53 expression alters cardiac-derived mesenchymal stromal cell fate decisions. PMID: 27501845
  30. These results are the first evidence that the inhibition of HDAC1 by (S)-2 downregulates CIP2A transcription PMID: 27029072
  31. data showed that HDAC1 can trigger the proliferation and migration of breast cancer cells via activation of Snail/IL-8 signals PMID: 28779562
  32. Mechanical stimulation orchestrates the osteogenic differentiation of human bone marrow stromal cells by regulating HDAC1 PMID: 27171263
  33. High HDAC1 expression is associated with Multidrug Resistance in breast and cervical cancer. PMID: 28716899
  34. these results suggest that HDAC1 and HDAC6 may play a role in clear cell renal cell carcinoma biology PMID: 27506904
  35. Histone deacetylase 1 regulates the expression of progesterone receptor A during human parturition by occupying the progesterone receptor A promoter. PMID: 26758364
  36. High HDAC1 expression may contribute to the aggressiveness of human breast cancer with cytoplasmic-only expression of maspin PMID: 28870936
  37. Results indicate that HCV core induced epithelial-mesenchymal transition (EMT) by interacting with the transcriptional repressor complex Snail/HDAC1/2 at the E-cadherin promoter, which led to E-cadherin repression and increased invasiveness of hepatoma cells. PMID: 26549030
  38. Coexpression of SALL4 with HDAC1 and/or HDAC2 was associated with PTEN underexpression and a poor prognosis in hepatocellular carcinoma. PMID: 28411180
  39. Acetylation-dependent control of global poly(A) RNA degradation by CBP/p300 and HDAC1-HDAC2 has been described. PMID: 27635759
  40. Meta-analysis results suggest that HDAC1 mRNA or protein expression may serve as a good diagnostic and prognostic marker for lung cancer. PMID: 28767587
  41. Histone deacetylase assays confirmed that MIER2, but not MIER3 complexes, have associated deacetylase activity. PMID: 28046085
  42. High HDAC1 expression is associated with drug Resistance in Malignant Melanoma. PMID: 26980768
  43. HDAC1 overexpression is associated with Breast Cancer. PMID: 27197203
  44. Data suggest that epigenetic changes in histone acetylation and DNA methylation may contribute to the repression of RGS2 (regulator of G-protein signaling 2) expression in chemo-resistant ovarian cancer cells; regulation of HDAC1 (histone deacetylase 1) and DNMT1 (DNA methyltransferase 1) contribute to the suppression of RGS2. PMID: 28102109
  45. class I HDACs (HDAC1, 2, 3 and 8) play a major role in regulating extracellular matrix and Epithelial-mesenchymal transition, whereas class IIa HDACs (HDAC4 and 5) are less effective. PMID: 27420561
  46. We found that TCF7L1 recruits the C-terminal binding protein (CtBP) and histone deacetylase 1 (HDAC1) to the DKK4 promoter to repress DKK4 gene expression. In the absence of TCF7L1, TCF7L2 and beta-catenin occupancy at the DKK4 promoter is stimulated and DKK4 expression is increased. These findings uncover a critical role for TCF7L1 in repressing DKK4 gene expression to promote the oncogenic potential of CRCs. PMID: 28450117
  47. Using mass spectrometry and site directed mutagenesis, a new Sp1 phosphorylation site Ser702 was defined to be associated with Sp1-HDAC1 interaction and may be important in SR-BI activation, shedding light on the knowledge of delicate mechanism of hepatic HDL receptor SR-BI gene modulation by LDL. PMID: 27320013
  48. findings indicate that WRN interacts with HDACs 1 and 2 to facilitate activity of stalled replication forks under conditions of replication stress. PMID: 27672210
  49. HDAC1- and SRC-mediated phosphorylation of RUNX3 induced by oxidative stress is associated with the cytoplasmic localization of RUNX3 and can lead to RUNX3 inactivation and carcinogenesis. PMID: 27990641
  50. Cutaneous T-cell lymphoma (CTCL) pathogenesis remains unknown, and there are no curative therapies. Our findings not only demonstrate a critical role for IL15-mediated inflammation in cutaneous T-cell lymphomagenesis, but also uncover a new oncogenic regulatory loop in CTCL involving IL15, HDAC1, HDAC6, and miR-21 that shows differential sensitivity to isotype-specific HDAC inhibitors PMID: 27422033
  51. Increased cancer-specific mortality was significantly associated with HDAC2 expression, mortality was also increased with high HDAC1 expression. PMID: 26352599
  52. HDAC1 upregulation is associated with hepatocellular carcinoma. PMID: 27342975
  53. HDAC1 expression in TE-1, Eca109 and EC9706 cells was significantly higher compared with normal esophageal cell line HEEC.The siRNA-mediated HDAC1 knockdown significantly inhibited the proliferation, migration and invasion of TE-1 cells probably by regulating the expression of cell cycle- and EMT-related proteins. PMID: 27086779
  54. High HDAC1 expression is associated with osteosarcoma progression. PMID: 26797758
  55. HDACs 1 and 2 are recruited by the MTA1 corepressor to form the catalytic core of the complex. PMID: 27098840
  56. Data indicate that bombesin receptor-activated protein (BRAP) might increase histone deacetylases 1/2 (HDAC) activity that leads to NF-kappa B (NF-kappaB) activation via its putative C-terminal domain. PMID: 26460487
  57. Results found an involvement of epigenetic processes in SNAIL-induced epithelial to mesenchymal transition, highlighted by a cross-talk between SNAIL and the histone deacetylase HDAC1, and activation of the AKT pathway in breast cancer cells. PMID: 26764010
  58. Data show that histone H2B of prostate cancer cell line DU-145 have hypoacetylation, hypomethylation, and dephosphorylation, suggesting there was an excessive histone deacetylase activity in the cells. PMID: 26759222
  59. the expression of HDAC1 was significantly higher in LRS than that in HRS. The positive rates for stage III-IV tumor were significantly higher than those for stage II. PMID: 26588579
  60. HDAC1 and DAXX are co-repressors associated with epigenetic regulation that help to control promoter histone acetylation reactions involved in regulating GAD67. PMID: 26812044
  61. RORgammat is acetylated, and this acetylation is reciprocally regulated by the histone acetyltransferase p300 and the histone deacetylase HDAC1. PMID: 26549310
  62. We demonstrate that HDAC1 is a component of host innate antiviral response against IAV, and IAV undermines HDAC1 to limit its role in antiviral response. PMID: 26912629
  63. Furthermore, a direct interaction between AnkA and HDAC1 was detected at the CYBB promoter, and was critical for AnkA-mediated CYBB repression. PMID: 25996657
  64. Study shows evidence implicating HDAC1 as an important regulator of tissue damage in rheumatoid arthritis (RA). Its inhibition in RA synovial fibroblasts results in attenuation of the autoaggressive phenotype as reduced proliferation and migration. PMID: 26152200
  65. Enhanced HDAC-1 expression in Pancreatic Adenocarcinoma was significantly associated with increased tumor proliferative capacity, borderline with the absence of lymph node metastases, and showed longer survival times compared to those with low expression. PMID: 26502922
  66. These observations indicate a HDAC1-mediated IL-12B gene expression suppression by live, virulent Mycobacterium tuberculosis to subvert the immune system to survive and replicate in the host. PMID: 26697414
  67. Results show that mRNA expression of histone deacetylases HDAC1 and HDAC2 were significantly increased in peripheral blood mononuclear cells of patients with with Graves' disease compared to controls. PMID: 26116233
  68. Results indicate that HDAC1 depletion inhibits the metastatic abilities of gastric cancer cells by regulating the miRNA-34a/CD44 pathway, suggesting that HDAC1 may be a potential target for the treatment of gastric cancer. PMID: 26035691
  69. DW22 antitumor agent upregulates RXRa and downregulates histone deacetylase in lung cancer and breast cancer tissues and cell lines PMID: 25762635
  70. Histone deacetylases 1 and 2 have roles in regulating DNA replication and DNA repair in cancer [review] PMID: 25942572
  71. Increase of HDAC1 acetylation and reduced level of SIRT1 protein during cellular stress directly link to the induction of p53 acetylation. Results unveil the mechanism underlying the dynamic regulation of p53 acetylation during cell stress. PMID: 25950477
  72. shRNA-mediated knockdown of HDAC1 or HDAC2 resulted in growth inhibition of B-cell acute lymphoblastic leukemia cells. PMID: 25688158
  73. Overexpression of histone deacetylase 1 was associated with lung adenocarcinoma. PMID: 25279705
  74. This study shown HDAC1 to be the risk factor for attention deficit/hyperactivity disorder. PMID: 25840828
  75. curcumin and PCI-34058-mediated ligand-dependent HDAC1 tunnel closure interferes negatively with the ASP-HIS charge relay system in HDAC1 PMID: 25860111
  76. Epigenetic silencing of HDAC1 by miR-449a upregulates Runx2 and promotes osteoblast differentiation. PMID: 25405810
  77. Here we demonstrate in the rat hippocampus that ongoing experience powerfully modifies the molecular response to one such intervention, histone deacetylase inhibitor (HDACi) administration. PMID: 26290249
  78. 5-HTT gene is epigenetically downregulated by HDAC1 in several types of cancer. PMID: 26024595
  79. simultaneous depletion of HDAC1/2 compromises the deacetylation of H3K56Ac, while depletion of HDAC1 or HDAC2 alone has no effect on H3K56Ac. PMID: 26255936
  80. CD133 expression and THBS1 expression were prognostic factors, and a negative relationship between HDAC and THBS1 was observed in advanced gastric cancer. PMID: 25862862
  81. Gefitinib-resistant lung cancer cells show HDAC1 overexpression, and its knockdown sensitizes resistant cells to gefitinib. PMID: 25593344
  82. Overexpression of HDAC1 is associated with poor prognosis in multiple myeloma patients. PMID: 25482492
  83. HDAC1 overexpression was associated with prostate cancer recurrence, rapid tumor cell proliferation and genomic instability. PMID: 25794974
  84. HDAC1 and Klf4 are potential new molecular markers and targets for clinical diagnosis, prognosis, and treatment of myeloid leukemia. PMID: 25341045
  85. Transgenic mice expressing mono-allelic Hdac1 (or Hdac2) reveals that Hdac1 and Hdac2 contribute differently to the development of specific hematopoietic lineages. PMID: 24763403
  86. importance of class I HDACs in the muscle atrophy program and indicate that class I HDAC inhibitors are feasible countermeasures to impede muscle atrophy and weakness. PMID: 24463822
  87. Study identifies the miR-34a-HDAC1/HDAC7-HSP70 K246 axis as a novel molecular signature predictive of therapy resistance. PMID: 25173798
  88. nuclear targeting of MIER1alpha requires an intact ELM2 domain and is dependent on interaction with HDAC1/2 PMID: 24376786
  89. The transcriptional function of HCS was shown by in vitro pull down and in vivo co-immunoprecipitation assays to depend on its interaction with the histone deacetylases HDAC1, HDAC2 and HDAC7 PMID: 24239178
  90. Our results indicate that HDAC1 plays an important role in chondrogenesis and may represent a therapeutic target for modulation of cartilage development. PMID: 25445594
  91. Data indicate that the 20(S)-ginsenoside Rh2 [Rh2(S) can inhibit the proliferation of K562 cells and induce its cycle arrest and apoptosis through inhibiting histone deacetylases HDAC1 and HDAC2 activity. PMID: 25270209
  92. novel HDAC1/4/miR-200b/E2F3 signaling contributes to chemoresistance of human lung adenocarcinoma cells PMID: 24830600
  93. Data support a model wherein DDB1 and DDB2 cooperate to repress Bcl-2 transcription. DDB2 recognizes and binds to the Bcl-2 P1 promoter, and HDAC1 is recruited through the DDB1 subunit associated with DDB2 to deacetylate histone H3K9. PMID: 24249678
  94. PP1alpha and class I histone deacetylase (HDAC1/2/3) signaling pathways are essential for the stress-induced BRD4 release from chromatin. PMID: 24939842
  95. Inhibition of HDAC1 and DNMT1 modulate RGS10 expression and decrease ovarian cancer chemoresistance. PMID: 24475290
  96. Data suggest that direct interactions of HLCS (holocarboxylase synthetase) with NCOR1 (nuclear receptor corepressor 1) and HDAC1 (histone deacetylase 1) contribute toward transcriptional repression of repeats, presumably increasing genome stability. PMID: 24840043
  97. Findings uncover a role for paralog-specific sumoylation of HDAC1 whose significance is emphasized by the use of HDAC inhibitors as anticancer drugs. PMID: 24068740
  98. Various subunits of T complex proteins (TCP1) and prefoldin proteins (PFDN) were identified as interacting partners that showed high affinity with HDAC1 in HepG2 cells. PMID: 23621261
  99. a role for miR-449b in regulation of HDAC1 and antiviral cytokine signaling PMID: 24086750
  100. CHD4 and HDAC1 occupy the promoters of several of these hypermethylated tumor suppressor genes and physically and functionally interact to maintain their silencing. PMID: 23708667

Show More

Hide All

Subcellular Location Nucleus
Protein Families Histone deacetylase family, HD type 1 subfamily
Tissue Specificity Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
Database Links

HGNC: 4852

OMIM: 601241

KEGG: hsa:3065

STRING: 9606.ENSP00000362649

UniGene: Hs.88556

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1