MR1 Antibody

Code CSB-PA853268LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: THP-1 whole cell lysate, K-562 whole cell lysate, Raji whole cell lysate, Hela whole cell lysate, A2780 whole cell lysate, Mouse Brain tissue
    All lanes: MR1 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 40, 35, 29, 26 kDa
    Observed band size: 48 kDa
  • IHC image of CSB-PA853268LA01HU diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • Immunofluorescence staining of HepG2 cells with CSB-PA853268LA01HU at 1:200, counter-stained with DAPI.The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details

Description
MR1 polyclonal antibody CSB-PA853268LA01HU was produced in the rabbits immunized by using the Recombinant Human Major histocompatibility complex class I-related gene protein (150-253AA) as the immunogen. The target protein MR1 is a antigen-presenting molecule specialized in presenting metabolites of microbial vitamin B to mucosal-associated invariant T-cells (MAITs). MAIT lymphocytes are preferentially located in the gut lamina propria and therefore may be involved in monitoring commensal flora or serve as a distress signal. Diseases associated with MR1 include Hemochromatosis, Type 1 and Microscopic Colitis.
This Rabbit anti-Homo sapiens (Human) MR1 Polyclonal antibody was tested in the ELISA, WB, IHC and IF applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It only reacts with the MR1 proteins of human-origin and may be used to detect the endogenous levels of MR1 protein.
Full Product Name Rabbit anti-Homo sapiens (Human) MR1 Polyclonal antibody
Uniprot No. Q95460
Target Names MR1
Alternative Names Class I histocompatibility antigen like protein antibody; Class I histocompatibility antigen-like protein antibody; HLALS antibody; HMR1_HUMAN antibody; Major histocompatibility complex class I like sequence antibody; Major histocompatibility complex class I related antibody; Major histocompatibility complex class I related gene protein antibody; Major histocompatibility complex class I related isoform CRA a antibody; Major histocompatibility complex class I related isoform CRA c antibody; Major histocompatibility complex class I related isoform CRA d antibody; Major histocompatibility complex class I-related gene protein antibody; MHC class I like antigen MR 1 antibody; MHC class I like antigen MR1 antibody; MHC class I related gene protein antibody; MHC class I related protein 1 isoform B antibody; MHC class I related protein 1 isoform C antibody; MHC class I-related gene protein antibody; MR 1 antibody; Mr1 antibody; Mr1 protein antibody; MR1B antibody; MR1C antibody
Raised in Rabbit
Species Reactivity Human, Mouse
Immunogen Recombinant Human Major histocompatibility complex class I-related gene protein (150-253AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
(From Uniprot)
Antigen-presenting molecule specialized in displaying microbial pyrimidine-based metabolites to alpha-beta T cell receptors (TCR) on innate-type mucosal-associated invariant T (MAIT) cells. In complex with B2M preferentially presents riboflavin-derived metabolites to semi-invariant TRAV1-2 TCRs on MAIT cells, guiding immune surveillance of the microbial metabolome at mucosal epithelial barriers. Signature pyrimidine-based microbial antigens are generated via non-enzymatic condensation of metabolite intermediates of the riboflavin pathway with by-products arising from other metabolic pathways such as glycolysis. Typical potent antigenic metabolites are 5-(2-oxoethylideneamino)-6-D-ribitylaminouracil (5-OE-RU) and 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil (5-OP-RU), products of condensation of 5-amino-6-D-ribityaminouracil (5-A-RU) with glyoxal or methylglyoxal by-products, respectively. May present microbial antigens to various TRAV1-2-negative MAIT cell subsets, providing for unique recognition of diverse microbes, including pathogens that do not synthesize riboflavin. Upon antigen recognition, elicits rapid innate-type MAIT cell activation to eliminate pathogenic microbes by directly killing infected cells. During T cell development, drives thymic selection and post-thymic terminal differentiation of MAIT cells in a process dependent on commensal microflora. Acts as an immune sensor of cancer cell metabolome. May present a tumor-specific or -associated metabolite essential for cancer cell survival to a pan-cancer TCR consisting of TRAV38.2-DV8*TRAJ31 alpha chain paired with a TRBV25.1*TRBJ2.3 beta chain on a non-MAIT CD8-positive T cell clone (MC.7.G5), triggering T cell-mediated killing of a wide range of cancer cell types.
Gene References into Functions
  1. Human mucosal-associated invariant T cells possess capacity for B cell help via MR1-mediated immunoglobulins production. PMID: 28807929
  2. Early endosomal TLR9 activation is important for MR1-mediated bacterial antigen presentation in B cells. PMID: 28518215
  3. Polymorphism in MR1 gene is associated with susceptibility to tuberculosis. PMID: 27881839
  4. we have shown that MR1-mediated MAIT cell activation is tightly regulated at several levels. Efficient MR1-mediated MAIT cell activation requires both intact bacteria to access an acidified endolysomal compartment and activation of the APC through NF-kappaB or interferon signaling pathways. PMID: 27105778
  5. results suggest that high expression of MR-1 is involved in hepatocellular carcinoma progression PMID: 26823810
  6. Data show that the major histocompatibility complex, class I-related protein (MR1) antigen presentation is characterized by a rapid 'off-on-off' mechanism that is strictly dependent on antigen availability. PMID: 27043408
  7. endosome-mediated trafficking of MR1 allows for selective sampling of the intracellular environment PMID: 27031111
  8. Studies indicate that the antigen-presenting molecules CD1 and MHC class I-related protein (MR1) display lipids and small molecules to T cells. PMID: 26388332
  9. Data indicate that high expression of CD26 ia a specific markers to define major histocompatibility complex, class I-like sequence protein MR1-restricted mucosa-associated invariant T (MAIT) cells. PMID: 25752900
  10. Mucosal-associated invariant T cell adaptation was a direct consequence of exposure to various exogenous MR1-restricted epitopes. PMID: 25049333
  11. [review] Mucosal-associated invariant T-cell (MAIT) T-cell antigen receptor recognizes riboflavin and folic acid metabolites bound by MR1 in a conserved docking mode and thus acts like a pattern recognition receptor. PMID: 24556396
  12. MR1 tetramers allow precise phenotypic characterization of human and mouse mucosal-associated invariant T cells. PMID: 24101382
  13. A novel MR1B isoform probably plays a physiological role complementary to MR1A with respect to mucosal-associated invariant T cells development and/or function. PMID: 23457030
  14. Human thymic MR1-restricted MAIT cells are innate pathogen-reactive effectors that adapt following thymic egress. PMID: 22692454
  15. metabolites of vitamin B represent a class of antigen that are presented by MR1 for MAIT-cell immunosurveillance; as many vitamin biosynthetic pathways are unique to bacteria and yeast, data suggest that MAIT cells use these metabolites to detect microbial infection PMID: 23051753
  16. Mutagenesis of MR1 showed that only two residues, which were centrally positioned and on opposing sides of the antigen-binding cleft of MR1, were essential for MAIT cell activation PMID: 22412157
  17. Taken together these results strongly suggest that MR1 needs to bind proteasome-independent ligands in order to properly reach the cell surface. PMID: 21777569
  18. fact that MR1 seems mainly intracellular might offer clues as to the process, given the precedence in other class I molecules PMID: 21190736
  19. MR1 molecules can associate with the peptide-loading complex and can be detected at low levels at the surface of transfected cells. PMID: 12794138
  20. Our results demonstrated a preferential association of MR1 with beta(2)-microglobulin in MHC class I-deficient B cell lines. PMID: 18068122
  21. the presentation pathway of MR1 to MAIT cells is highly evolutionarily conserved PMID: 19416870
  22. Induction of MHC class I-like antigen is associated with relapsed chronic myeloid leukemia. PMID: 19706888
  23. a function for MR1 in the development of IgA producing plasma cells. PMID: 19760593

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Subcellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein.; [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.; [Isoform 3]: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.; [Isoform 4]: Secreted.
Protein Families MHC class I family
Tissue Specificity Ubiquitous. Low expression is detected in peripheral blood B cells, T cells, monocytes and in bronchial epithelial cells (at protein level). Expressed in plasmablasts or plasma B cells in the lamina propria of ileum, appendix and colon (at protein level).
Database Links

HGNC: 4975

OMIM: 600764

KEGG: hsa:3140

STRING: 9606.ENSP00000356552

UniGene: Hs.13500

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