Name: MR1 Antibody
Brand: CUSABIO
SKU: CSB-PA853268LA01HU

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MR1 Polyclonal antibody

Uniprot No.

Q95460

Target Names

MR1

Alternative Names

Class I histocompatibility antigen like protein antibody; Class I histocompatibility antigen-like protein antibody; HLALS antibody; HMR1_HUMAN antibody; Major histocompatibility complex class I like sequence antibody; Major histocompatibility complex class I related antibody; Major histocompatibility complex class I related gene protein antibody; Major histocompatibility complex class I related isoform CRA a antibody; Major histocompatibility complex class I related isoform CRA c antibody; Major histocompatibility complex class I related isoform CRA d antibody; Major histocompatibility complex class I-related gene protein antibody; MHC class I like antigen MR 1 antibody; MHC class I like antigen MR1 antibody; MHC class I related gene protein antibody; MHC class I related protein 1 isoform B antibody; MHC class I related protein 1 isoform C antibody; MHC class I-related gene protein antibody; MR 1 antibody; Mr1 antibody; Mr1 protein antibody; MR1B antibody; MR1C antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Major histocompatibility complex class I-related gene protein (150-253AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MR1 Antibody (Product code: CSB-PA853268LA01HU) is Non-conjugated. For MR1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA853268LB01HU	MR1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA853268LC01HU	MR1 Antibody, FITC conjugated
Biotin	CSB-PA853268LD01HU	MR1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

MR1 polyclonal antibody CSB-PA853268LA01HU was harvested from the rabbits immunized with the recombinant human major histocompatibility complex class I-related gene protein (150-253aa). The target protein MR1 mainly alerts the immune system to the presence of infected or malignant cells that express the stress-induced self-antigens and presents these abnormal cells to natural killer (NK) cells and some T cells for destruction.

This rabbit anti-human MR1 polyclonal antibody has validated the specificity and reliability in the ELISA, WB, IHC, and IF applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It only reacts with the human MR1 protein.

Target Background

Function

Antigen-presenting molecule specialized in displaying microbial pyrimidine-based metabolites to alpha-beta T cell receptors (TCR) on innate-type mucosal-associated invariant T (MAIT) cells. In complex with B2M preferentially presents riboflavin-derived metabolites to semi-invariant TRAV1-2 TCRs on MAIT cells, guiding immune surveillance of the microbial metabolome at mucosal epithelial barriers. Signature pyrimidine-based microbial antigens are generated via non-enzymatic condensation of metabolite intermediates of the riboflavin pathway with by-products arising from other metabolic pathways such as glycolysis. Typical potent antigenic metabolites are 5-(2-oxoethylideneamino)-6-D-ribitylaminouracil (5-OE-RU) and 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil (5-OP-RU), products of condensation of 5-amino-6-D-ribityaminouracil (5-A-RU) with glyoxal or methylglyoxal by-products, respectively. May present microbial antigens to various TRAV1-2-negative MAIT cell subsets, providing for unique recognition of diverse microbes, including pathogens that do not synthesize riboflavin. Upon antigen recognition, elicits rapid innate-type MAIT cell activation to eliminate pathogenic microbes by directly killing infected cells. During T cell development, drives thymic selection and post-thymic terminal differentiation of MAIT cells in a process dependent on commensal microflora. Acts as an immune sensor of cancer cell metabolome. May present a tumor-specific or -associated metabolite essential for cancer cell survival to a pan-cancer TCR consisting of TRAV38.2-DV8*TRAJ31 alpha chain paired with a TRBV25.1*TRBJ2.3 beta chain on a non-MAIT CD8-positive T cell clone (MC.7.G5), triggering T cell-mediated killing of a wide range of cancer cell types.

Gene References into Functions

Human mucosal-associated invariant T cells possess capacity for B cell help via MR1-mediated immunoglobulins production. PMID: 28807929
Early endosomal TLR9 activation is important for MR1-mediated bacterial antigen presentation in B cells. PMID: 28518215
Polymorphism in MR1 gene is associated with susceptibility to tuberculosis. PMID: 27881839
we have shown that MR1-mediated MAIT cell activation is tightly regulated at several levels. Efficient MR1-mediated MAIT cell activation requires both intact bacteria to access an acidified endolysomal compartment and activation of the APC through NF-kappaB or interferon signaling pathways. PMID: 27105778
results suggest that high expression of MR-1 is involved in hepatocellular carcinoma progression PMID: 26823810
Data show that the major histocompatibility complex, class I-related protein (MR1) antigen presentation is characterized by a rapid 'off-on-off' mechanism that is strictly dependent on antigen availability. PMID: 27043408
endosome-mediated trafficking of MR1 allows for selective sampling of the intracellular environment PMID: 27031111
Studies indicate that the antigen-presenting molecules CD1 and MHC class I-related protein (MR1) display lipids and small molecules to T cells. PMID: 26388332
Data indicate that high expression of CD26 ia a specific markers to define major histocompatibility complex, class I-like sequence protein MR1-restricted mucosa-associated invariant T (MAIT) cells. PMID: 25752900
Mucosal-associated invariant T cell adaptation was a direct consequence of exposure to various exogenous MR1-restricted epitopes. PMID: 25049333
[review] Mucosal-associated invariant T-cell (MAIT) T-cell antigen receptor recognizes riboflavin and folic acid metabolites bound by MR1 in a conserved docking mode and thus acts like a pattern recognition receptor. PMID: 24556396
MR1 tetramers allow precise phenotypic characterization of human and mouse mucosal-associated invariant T cells. PMID: 24101382
A novel MR1B isoform probably plays a physiological role complementary to MR1A with respect to mucosal-associated invariant T cells development and/or function. PMID: 23457030
Human thymic MR1-restricted MAIT cells are innate pathogen-reactive effectors that adapt following thymic egress. PMID: 22692454
metabolites of vitamin B represent a class of antigen that are presented by MR1 for MAIT-cell immunosurveillance; as many vitamin biosynthetic pathways are unique to bacteria and yeast, data suggest that MAIT cells use these metabolites to detect microbial infection PMID: 23051753
Mutagenesis of MR1 showed that only two residues, which were centrally positioned and on opposing sides of the antigen-binding cleft of MR1, were essential for MAIT cell activation PMID: 22412157
Taken together these results strongly suggest that MR1 needs to bind proteasome-independent ligands in order to properly reach the cell surface. PMID: 21777569
fact that MR1 seems mainly intracellular might offer clues as to the process, given the precedence in other class I molecules PMID: 21190736
MR1 molecules can associate with the peptide-loading complex and can be detected at low levels at the surface of transfected cells. PMID: 12794138
Our results demonstrated a preferential association of MR1 with beta(2)-microglobulin in MHC class I-deficient B cell lines. PMID: 18068122
the presentation pathway of MR1 to MAIT cells is highly evolutionarily conserved PMID: 19416870
Induction of MHC class I-like antigen is associated with relapsed chronic myeloid leukemia. PMID: 19706888
a function for MR1 in the development of IgA producing plasma cells. PMID: 19760593

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein.; [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.; [Isoform 3]: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.; [Isoform 4]: Secreted.

Protein Families

MHC class I family

Tissue Specificity

Ubiquitous. Low expression is detected in peripheral blood B cells, T cells, monocytes and in bronchial epithelial cells (at protein level). Expressed in plasmablasts or plasma B cells in the lamina propria of ileum, appendix and colon (at protein level).

Database Links

HGNC: 4975

OMIM: 600764

KEGG: hsa:3140

STRING: 9606.ENSP00000356552

UniGene: Hs.13500

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Code	CSB-PA853268LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: THP-1 whole cell lysate, K-562 whole cell lysate, Raji whole cell lysate, Hela whole cell lysate, A2780 whole cell lysate, Mouse Brain tissue All lanes: MR1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 35, 29, 26 kDa Observed band size: 48 kDa IHC image of CSB-PA853268LA01HU diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 cells with CSB-PA853268LA01HU at 1:200, counter-stained with DAPI.The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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MR1 Antibody

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