NUMA1 Antibody

^Datasheet

Specification
Target

Code	CSB-PA016185YA01HU
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Size	US$299Purchase it in Cusabio online store (only available for customers from the US)
Uniprot No.	Q14980
Image	Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate All lanes: NUMA1 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 239, 237, 202, 201, 110 kDa Observed band size: 239 kDa Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA016185YA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA016185YA01HU at dilution of 1:100 Immunofluorescence staining of MCF-7 cells with CSB-PA016185YA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunogen	Recombinant Human Nuclear mitotic apparatus protein 1 protein (627-870AA)
Raised in	Rabbit
Species Reactivity	Human
Tested Applications	ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Relevance	Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority if the nuclear volume (PubMed:10075938). Required for maintenance and establishment of the mitotic spindle poles during symmetric cell divisions, functioning as a tether linking bulk microtubules of the spindle to centrosomes (PubMed:7769006, PubMed:11956313, PubMed:26195665). Also required for proper alignment of the mitotic spindle during asymmetric cell divisions (PubMed:21816348).
Form	Liquid
Conjugate	Non-conjugated
Storage Buffer	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method	>95%, Protein G purified
Isotype	IgG
Clonality	Polyclonal
Alias	Nuclear mitotic apparatus protein 1 (Nuclear matrix protein-22) (NMP-22) (Nuclear mitotic apparatus protein) (NuMA protein) (SP-H antigen), NUMA1, NMP22 NUMA
Immunogen Species	Human
Protocols	ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol
Research Area	Cell Biology
Target Names	NUMA1
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function

Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division

Subcellular Location

Nucleus, Nucleus, nucleoplasm, Nucleus matrix, Chromosome, Cytoplasm, cytoskeleton, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole, Cytoplasm, cell cortex, Cell membrane, Lipid-anchor, Cytoplasmic side, Lateral cell membrane, Note=Mitotic cell cycle-dependent shuttling protein that relocalizes from the interphase nucleus to the spindle poles and cell cortex (PubMed:1541636, PubMed:10811826), In interphase, resides in the nuclear matrix (PubMed:1541630, PubMed:1541636, PubMed:23921553), In prophase, restricted to the interchromatin or condensed chromosome space (PubMed:10811826), In prometaphase, after nuclear envelope disassembly, forms aggregates both in the spindle midzone and at duplicated centrosomes and astral microtubules (MTs) of the bipolar spindle apparatus (PubMed:10811826), Translocates from the spindle midzone towards the spindle poles along spindle fibers in a MT- and dynein-dynactin-dependent manner until the anaphase onset (PubMed:1541636, PubMed:10811826), In metaphase, recruited to the polar cortical region in a GPSM2- and GNAI1-dependent manner (PubMed:23870127, PubMed:24109598, PubMed:24996901), Excluded from the metaphase equatorial cortical region in a RanGTP-dependent manner (PubMed:22327364, PubMed:23870127), Phosphorylation on Thr-2055 by CDK1 results in its localization at spindle poles in metaphase, but not at the cell cortex (PubMed:23921553), In anaphase, recruited and anchored at the cell membrane of the polar cortical region in a EPB41-, EPB41L2-, phosphatidylinositol-dependent and GPSM2- and G(i) alpha proteins-independent manner (PubMed:23870127, PubMed:24996901, PubMed:24109598, PubMed:24371089), Excluded from the anaphase equatorial region of the cell cortex in a RACGAP1- and KIF23-dependent and RanGTP-independent manner (PubMed:24996901), Associated with astral MTs emanating from the spindle poles during anaphase (PubMed:12445386, PubMed:24996901), Nonphosphorylated Thr-2055 localizes at the cell cortex, weakly during metaphase and more prominently during anaphase in a phosphatase PPP2CA-dependent manner (PubMed:23921553), As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident (PubMed:1541636), Localizes to the tips of cortical MTs in prometaphase (PubMed:26765568), Localizes along MTs and specifically to both MT plus and minus ends (PubMed:26765568), Accumulates also at MT tips near the cell periphery (PubMed:26765568), Colocalizes with GPSM2 at mitotic spindle poles during mitosis (PubMed:11781568, PubMed:21816348), Colocalizes with SPAG5 at mitotic spindle at prometaphase and at mitotic spindle poles at metaphase and anaphase (PubMed:27462074), Colocalizes with ABRO1 at mitotic spindle poles (PubMed:26195665), Colocalized with TNKS from prophase through to anaphase in mitosis (PubMed:16076287), Colocalizes with tubulin alpha (PubMed:12445386), CCSAP is essential for its centrosomal localization (PubMed:26562023), In horizontally retinal progenitor dividing cells, localized to the lateral cortical region (By similarity), SUBCELLULAR LOCATION: Isoform 3: Cytoplasm, cytosol, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole, Note=During interphase, mainly clustered at the centrosomal region in the cytosol, After entry into mitosis, detected at mitotic spindle poles, SUBCELLULAR LOCATION: Isoform 4: Cytoplasm, cytosol, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole

Database Links

HGNC: 8059

OMIM: 164009

KEGG: hsa:4926

STRING: 9606.ENSP00000377298

UniGene: Hs.325978

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Code	Product Name	Species Reactivity	Application
CSB-PA016185GA01HU	NUMA1 Antibody	Human,Mouse,Rat	ELISA,WB
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NUMA1 Proteins

NUMA1 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP016185HU CSB-EP016185HU CSB-BP016185HU CSB-MP016185HU	Recombinant Human Nuclear mitotic apparatus protein 1(NUMA1) ,partial	Yeast E.coli Baculovirus Mammalian cell

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NUMA1 ELISA Kit for Homo sapiens (Human)

Code	Product Name	Sample Types	Sensitivity
CSB-E09037h	Human Nuclear matrix protein 22,NMP-22 ELISA Kit	serum, plasma, urine	0.04 ng/mL

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NUMA1 Antibody

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