Name: NUMA1 Antibody
Brand: CUSABIO
SKU: CSB-PA016185YA01HU

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NUMA1 Polyclonal antibody

Uniprot No.

Q14980

Target Names

NUMA1

Alternative Names

Centrophilin stabilizes mitotic spindle in mitotic cells antibody; NMP 22 antibody; Nuclear matrix protein 22 antibody; Nuclear mitotic apparatus protein 1 antibody; Nuclear mitotic apparatus protein antibody; NUMA 1 antibody; NUMA antibody; NuMA protein antibody; NUMA1 antibody; NUMA1_HUMAN antibody; SP H antigen antibody; SP-H antigen antibody; Structural nuclear protein antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Nuclear mitotic apparatus protein 1 protein (627-870AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function (From Uniprot)	Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division
Gene References into Functions	It has been reported that the Galectin-3/NuMA interaction is functionally important for the spindle pole organization; spindle pole cohesion requires glycosylation-mediated localization of NuMA. PMID: 28469279 The p37 negatively regulates this function of PP1, resulting in lower cortical NuMA levels and correct spindle orientation. PMID: 29222185 The results show how E-cadherin instructs the assembly of the LGN/NuMA complex at cell-cell contacts, and define a mechanism that couples cell division orientation to intercellular adhesion. PMID: 28045117 Here, the authors use quantitative imaging and laser ablation to show that NuMA targets dynactin to spindle microtubule minus-ends, localizing dynein activity there. PMID: 29185983 All urine samples were analyzed by voided urine and bladder washing cytology, NMP22 and UBC rapid test (qualitatively and quantitatively). The best cutoff (highest Youden index; >/=6.7 ng/ml) for the quantitative UBC was determined by receiver operating characteristic curves. PMID: 28824318 Short isoform of NuMA might be functioned as a putative role of tumor suppressor. Further studies should be made to illuminate the relationship between ACTN4, MYBL2, and tumor progression. PMID: 28748856 The function of nuclear mitotic apparatus protein (NuMA) in rDNA transcription and p53-independent nucleolar stress response suggests a central role for NuMA in cellular homeostasis. PMID: 28981686 Importin-alpha/-beta regulates the NuMA functioning required for assembly of higher-order microtubule structures including the mitotic spindle. PMID: 28939615 Low post translational modifications of NuMA protein is associated with neoplasms. PMID: 28209915 Seven NuMA isoforms generated by alternative splicing were categorized into 3 groups: long, middle and short. Both exons 15 and 16 in long NuMA were "hotspot" for alternative splicing. Lower expression of short NuMA was observed in cancer cells compared with nonneoplastic controls. PMID: 25451259 Chimeric proteins constructed by fusion of LANA of Kaposi's sarcoma-associated herpesvirus with the NuMA could bind with ori-P and enhance replication of an ori-P-containing plasmid. PMID: 27829174 findings reveal a direct physical link between two important regulators of mitotic progression and demonstrate the critical role of the NuMA-Astrin interaction for accurate cell division. PMID: 27462074 Aurora-A governs the dynamic exchange between the cytoplasmic and the spindle pole-localized pools of NuMA. Aurora-A phosphorylates directly the C terminus of NuMA on three Ser residues, of which Ser1969 determines the dynamic behavior and the spindle orientation functions of NuMA. PMID: 26832443 Suppressor APC domain containing 2 negatively regulates the localization of LGN at the cell cortex, likely by competing with NuMA for its binding PMID: 26766442 Letter: risk factors for false positive results when using urinary NMP22 as biomarker for early detection of bladder cancer. PMID: 24976592 Results show that at low grade of disease, NMP22 test provided a significantly higher sensitivity for the detection of recurrent urothelial carcinoma of the bladder compared to voided urine cytology specimens. PMID: 25488052 NuMA interacts with phosphoinositides and links the mitotic spindle with the plasma membrane.During anaphase correct NuMA localization is mediated by direct membrane phospholipid binding. PMID: 24996901 Retinoblastoma protein (pRB) have a novel function in regulating the mitotic function of NuMA and spindle organization, which are required for proper cell cycle progression. PMID: 24350565 Study finds that frictional forces increase nonlinearly with microtubule-associated proteins (MAP) velocity across microtubules and depend on filament polarity, with NuMA's friction being lower when moving toward minus ends, EB1's lower toward plus ends, and PRC1's exhibiting no directional preference. PMID: 24725408 The mitosis-dependent dynamic SUMO-1 modification of NuMA might contribute to NuMA-mediated formation and maintenance of mitotic spindle poles during mitosis. PMID: 24309115 ectopic expression of NuMA can manipulate endogenous p53 and p21 transcriptional expression during interphase. PMID: 23828576 ectopic expression of BRAP2 inhibits nuclear localization of HMG20A and NuMA1, and prevents nuclear envelope accumulation of SYNE2. PMID: 23707952 Hepatocyte Par1b defines lumen position in concert with the position of the astral microtubule anchoring complex LGN-NuMA to yield the distinct epithelial division phenotypes. PMID: 24165937 NuMA phosphorylation by CDK1 couples mitotic progression with cortical dynein function. PMID: 23921553 Phosphorylation of NuMA by aurora-A is important for cell survival. PMID: 23097092 Data indicate that dynein- and astral microtubule-mediated transport of Galphai/LGN/nuclear mitotic apparatus (NuMA) complex from cell cortex to spindle poles. PMID: 23389635 nuclear matrix protein 22 (nuclear mitotic apparatus protein, NuMA) has a role in upper tract urothelial tumors. PMID: 21865670 NuMA is required for the recruitment of cyclin-dependent kinase 8, a component of the Mediator complex and a promoter of p53-mediated p21 gene function. PMID: 23589328 Studies indicate that the Inscuteable (Insc)and NuMA are mutually exclusive interactors of LGN. PMID: 22977735 Numa regulates spindle assemby in conjunction with Eg5. PMID: 23368718 NuMA expression was upregulated in tumours, with a significant association with disease stage in mucinous EOC subtypes, lymph node involvement and patient age PMID: 22719996 Low NUMA1 is associtated with glioblastoma. PMID: 22619067 Without functional NuMA, microtubules lose connection to meiosis I spindle poles, resulting in highly disorganized early spindle assembly. PMID: 22552228 During apoptotic rearrangement of interchromatin granule clusters, the nuclear matrix (NuMa rearrangement) and chromatin are closely associated. This process occurs in defined stages and depends on the activity of protein phosphatases, caspases and CAD. PMID: 22023725 The levels of urinary NMP22 and CK18 in the patients with transitional cell carcinoma of the bladder were significantly higher than those in the non-transitional cell carcinoma of the bladder. PMID: 19615282 Accurate distribution of NuMA is important for oocyte maturation, zygote and embryo development in humans. Proper assembly of NuMA is likely necessary for bipolar spindle organization and human oocyte developmental competence. PMID: 21297155 NuMA is expressed in interphase nuclei of fibroblasts and oocytes. PMID: 21406448 Phenotype onset is correlated with NuMA-RARalpha copy number; mice with higher copy number developing disease later than those with lower copy number. PMID: 21255834 These results suggest that NuMA may provide structural support in the interphase nucleus by contributing to the organization of chromatin. PMID: 20467816 Ric-8A and Gi alpha recruit LGN, NuMA, and dynein to the cell cortex to help orient the mitotic spindle. PMID: 20479129 Data suggest that pADPr provides a dynamic cross-linking function at spindle poles by extending from covalent modification sites on PARP-5a and NuMA and binding noncovalently to NuMA and that this function helps promote assembly of exactly two poles. PMID: 19759176 A domain within the C-terminal tail of NuMA interacts with tubulin and induces bundling and stabilisation of microtubules and leads to formation of abnormal mitotic spindles. PMID: 11956313 NuMA is cleaved differently in Jurkat T and HeLa cells, suggesting that different sets of caspases are activated in these cell lines. The normal diffuse intranuclear distribution of NuMA changed during apoptosis. PMID: 12508117 role in development of myelodi leukemia with promyelocytic features PMID: 14737102 Proteins and open reading frames with a NuMA C terminus distal portion like region were found in a diverse set of vertebrate species including mammals, birds, amphibia, and early teleost fish. PMID: 15388855 Multiple mechanisms regulate NUMA1 dynamics at spindle poles. PMID: 15561764 concluded that variations in the NuMA gene are likely responsible for the observed increased breast cancer risk PMID: 15684076 NuMA plays diverse important roles in vertebrate cells [review] PMID: 16146802 NuMA has a role in mammary epithelial differentiation by influencing the organization of chromatin. PMID: 17108325 point to the Rae1-NuMA interaction as a critical element for normal spindle formation in mitosis PMID: 17172455 Show More Hide All
Subcellular Location	Nucleus, Nucleus, nucleoplasm, Nucleus matrix, Chromosome, Cytoplasm, cytoskeleton, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole, Cytoplasm, cell cortex, Cell membrane, Lipid-anchor, Cytoplasmic side, Lateral cell membrane, Note=Mitotic cell cycle-dependent shuttling protein that relocalizes from the interphase nucleus to the spindle poles and cell cortex (PubMed:1541636, PubMed:10811826), In interphase, resides in the nuclear matrix (PubMed:1541630, PubMed:1541636, PubMed:23921553), In prophase, restricted to the interchromatin or condensed chromosome space (PubMed:10811826), In prometaphase, after nuclear envelope disassembly, forms aggregates both in the spindle midzone and at duplicated centrosomes and astral microtubules (MTs) of the bipolar spindle apparatus (PubMed:10811826), Translocates from the spindle midzone towards the spindle poles along spindle fibers in a MT- and dynein-dynactin-dependent manner until the anaphase onset (PubMed:1541636, PubMed:10811826), In metaphase, recruited to the polar cortical region in a GPSM2- and GNAI1-dependent manner (PubMed:23870127, PubMed:24109598, PubMed:24996901), Excluded from the metaphase equatorial cortical region in a RanGTP-dependent manner (PubMed:22327364, PubMed:23870127), Phosphorylation on Thr-2055 by CDK1 results in its localization at spindle poles in metaphase, but not at the cell cortex (PubMed:23921553), In anaphase, recruited and anchored at the cell membrane of the polar cortical region in a EPB41-, EPB41L2-, phosphatidylinositol-dependent and GPSM2- and G(i) alpha proteins-independent manner (PubMed:23870127, PubMed:24996901, PubMed:24109598, PubMed:24371089), Excluded from the anaphase equatorial region of the cell cortex in a RACGAP1- and KIF23-dependent and RanGTP-independent manner (PubMed:24996901), Associated with astral MTs emanating from the spindle poles during anaphase (PubMed:12445386, PubMed:24996901), Nonphosphorylated Thr-2055 localizes at the cell cortex, weakly during metaphase and more prominently during anaphase in a phosphatase PPP2CA-dependent manner (PubMed:23921553), As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident (PubMed:1541636), Localizes to the tips of cortical MTs in prometaphase (PubMed:26765568), Localizes along MTs and specifically to both MT plus and minus ends (PubMed:26765568), Accumulates also at MT tips near the cell periphery (PubMed:26765568), Colocalizes with GPSM2 at mitotic spindle poles during mitosis (PubMed:11781568, PubMed:21816348), Colocalizes with SPAG5 at mitotic spindle at prometaphase and at mitotic spindle poles at metaphase and anaphase (PubMed:27462074), Colocalizes with ABRO1 at mitotic spindle poles (PubMed:26195665), Colocalized with TNKS from prophase through to anaphase in mitosis (PubMed:16076287), Colocalizes with tubulin alpha (PubMed:12445386), CCSAP is essential for its centrosomal localization (PubMed:26562023), In horizontally retinal progenitor dividing cells, localized to the lateral cortical region (By similarity), SUBCELLULAR LOCATION: Isoform 3: Cytoplasm, cytosol, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole, Note=During interphase, mainly clustered at the centrosomal region in the cytosol, After entry into mitosis, detected at mitotic spindle poles, SUBCELLULAR LOCATION: Isoform 4: Cytoplasm, cytosol, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole
Database Links	HGNC: 8059 OMIM: 164009 KEGG: hsa:4926 STRING: 9606.ENSP00000377298 UniGene: Hs.325978

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Code	CSB-PA016185YA01HU
Size	US$299Purchase it in Cusabio online store (only available for customers from the US)
Image	Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate All lanes: NUMA1 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 239, 237, 202, 201, 110 kDa Observed band size: 239 kDa Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA016185YA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA016185YA01HU at dilution of 1:100 Immunofluorescence staining of MCF-7 cells with CSB-PA016185YA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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