NUMA1 Antibody, Biotin conjugated

Datasheet
Code CSB-PA016185YD01HU
Size US$299
Uniprot No. Q14980
Immunogen Recombinant Human Nuclear mitotic apparatus protein 1 protein (627-870AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA
Relevance Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority if the nuclear volume (PubMed:10075938). Required for maintenance and establishment of the mitotic spindle poles during symmetric cell divisions, functioning as a tether linking bulk microtubules of the spindle to centrosomes (PubMed:7769006, PubMed:11956313, PubMed:26195665). Also required for proper alignment of the mitotic spindle during asymmetric cell divisions (PubMed:21816348).
Form Liquid
Conjugate Biotin
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Nuclear mitotic apparatus protein 1 (Nuclear matrix protein-22) (NMP-22) (Nuclear mitotic apparatus protein) (NuMA protein) (SP-H antigen), NUMA1, NMP22 NUMA
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Research Area Cell Biology
Target Names NUMA1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division
Subcellular Location Nucleus, Nucleus, nucleoplasm, Nucleus matrix, Chromosome, Cytoplasm, cytoskeleton, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole, Cytoplasm, cell cortex, Cell membrane, Lipid-anchor, Cytoplasmic side, Lateral cell membrane, Note=Mitotic cell cycle-dependent shuttling protein that relocalizes from the interphase nucleus to the spindle poles and cell cortex (PubMed:1541636, PubMed:10811826), In interphase, resides in the nuclear matrix (PubMed:1541630, PubMed:1541636, PubMed:23921553), In prophase, restricted to the interchromatin or condensed chromosome space (PubMed:10811826), In prometaphase, after nuclear envelope disassembly, forms aggregates both in the spindle midzone and at duplicated centrosomes and astral microtubules (MTs) of the bipolar spindle apparatus (PubMed:10811826), Translocates from the spindle midzone towards the spindle poles along spindle fibers in a MT- and dynein-dynactin-dependent manner until the anaphase onset (PubMed:1541636, PubMed:10811826), In metaphase, recruited to the polar cortical region in a GPSM2- and GNAI1-dependent manner (PubMed:23870127, PubMed:24109598, PubMed:24996901), Excluded from the metaphase equatorial cortical region in a RanGTP-dependent manner (PubMed:22327364, PubMed:23870127), Phosphorylation on Thr-2055 by CDK1 results in its localization at spindle poles in metaphase, but not at the cell cortex (PubMed:23921553), In anaphase, recruited and anchored at the cell membrane of the polar cortical region in a EPB41-, EPB41L2-, phosphatidylinositol-dependent and GPSM2- and G(i) alpha proteins-independent manner (PubMed:23870127, PubMed:24996901, PubMed:24109598, PubMed:24371089), Excluded from the anaphase equatorial region of the cell cortex in a RACGAP1- and KIF23-dependent and RanGTP-independent manner (PubMed:24996901), Associated with astral MTs emanating from the spindle poles during anaphase (PubMed:12445386, PubMed:24996901), Nonphosphorylated Thr-2055 localizes at the cell cortex, weakly during metaphase and more prominently during anaphase in a phosphatase PPP2CA-dependent manner (PubMed:23921553), As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident (PubMed:1541636), Localizes to the tips of cortical MTs in prometaphase (PubMed:26765568), Localizes along MTs and specifically to both MT plus and minus ends (PubMed:26765568), Accumulates also at MT tips near the cell periphery (PubMed:26765568), Colocalizes with GPSM2 at mitotic spindle poles during mitosis (PubMed:11781568, PubMed:21816348), Colocalizes with SPAG5 at mitotic spindle at prometaphase and at mitotic spindle poles at metaphase and anaphase (PubMed:27462074), Colocalizes with ABRO1 at mitotic spindle poles (PubMed:26195665), Colocalized with TNKS from prophase through to anaphase in mitosis (PubMed:16076287), Colocalizes with tubulin alpha (PubMed:12445386), CCSAP is essential for its centrosomal localization (PubMed:26562023), In horizontally retinal progenitor dividing cells, localized to the lateral cortical region (By similarity), SUBCELLULAR LOCATION: Isoform 3: Cytoplasm, cytosol, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole, Note=During interphase, mainly clustered at the centrosomal region in the cytosol, After entry into mitosis, detected at mitotic spindle poles, SUBCELLULAR LOCATION: Isoform 4: Cytoplasm, cytosol, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole
Database Links

HGNC: 8059

OMIM: 164009

KEGG: hsa:4926

STRING: 9606.ENSP00000377298

UniGene: Hs.325978

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