Product Details

Uniprot No.

Target Names

NUMA1

Alternative Names

Nuclear mitotic apparatus protein 1 (Nuclear matrix protein-22) (NMP-22) (Nuclear mitotic apparatus protein) (NuMA protein) (SP-H antigen), NUMA1, NMP22 NUMA

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human NUMA1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

12A11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NUMA1, the nuclear mitotic apparatus protein 1, serves as a critical organizer of the mitotic spindle and plays essential roles in maintaining nuclear structure during interphase. This multifunctional protein coordinates microtubule organization at spindle poles during cell division, making it a valuable marker for researchers investigating cell cycle regulation, mitotic machinery, and nuclear architecture. Also recognized as NMP-22, NUMA1 has garnered significant attention in cell biology research for its involvement in chromosome segregation and its potential as a biomarker in certain pathological contexts.

This recombinant monoclonal antibody, clone 12A11, offers the reproducibility and consistency that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, this rabbit IgG eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The antibody was raised against a synthetic peptide derived from human NUMA1, ensuring precise epitope targeting.

Validation in flow cytometry demonstrates robust performance, with clear signal separation observed in HeLa cells at dilutions ranging from 1:50 to 1:200. The flow cytometry protocol employed fixation with formaldehyde followed by Triton X-100 permeabilization, appropriate for detecting this intracellular target. Acquisition of over 10,000 events confirmed reliable detection with minimal background when compared to isotype control conditions.

Supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C, this antibody arrives ready for integration into your cell biology research. Whether you are examining spindle assembly dynamics, characterizing nuclear organization, or exploring NUMA1 function in human cell models, this recombinant antibody provides the molecular specificity your experiments demand.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division. Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles. Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner. In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle. During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation. Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro. Also required for proper orientation of the mitotic spindle during asymmetric cell divisions. Plays a role in mitotic MT aster assembly. Involved in anastral spindle assembly. Positively regulates TNKS protein localization to spindle poles in mitosis. Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume. Required for epidermal differentiation and hair follicle morphogenesis.

Gene References into Functions

It has been reported that the Galectin-3/NuMA interaction is functionally important for the spindle pole organization; spindle pole cohesion requires glycosylation-mediated localization of NuMA. PMID: 28469279
The p37 negatively regulates this function of PP1, resulting in lower cortical NuMA levels and correct spindle orientation. PMID: 29222185
The results show how E-cadherin instructs the assembly of the LGN/NuMA complex at cell-cell contacts, and define a mechanism that couples cell division orientation to intercellular adhesion. PMID: 28045117
Here, the authors use quantitative imaging and laser ablation to show that NuMA targets dynactin to spindle microtubule minus-ends, localizing dynein activity there. PMID: 29185983
All urine samples were analyzed by voided urine and bladder washing cytology, NMP22 and UBC rapid test (qualitatively and quantitatively). The best cutoff (highest Youden index; >/=6.7 ng/ml) for the quantitative UBC was determined by receiver operating characteristic curves. PMID: 28824318
Short isoform of NuMA might be functioned as a putative role of tumor suppressor. Further studies should be made to illuminate the relationship between ACTN4, MYBL2, and tumor progression. PMID: 28748856
The function of nuclear mitotic apparatus protein (NuMA) in rDNA transcription and p53-independent nucleolar stress response suggests a central role for NuMA in cellular homeostasis. PMID: 28981686
Importin-alpha/-beta regulates the NuMA functioning required for assembly of higher-order microtubule structures including the mitotic spindle. PMID: 28939615
Low post translational modifications of NuMA protein is associated with neoplasms. PMID: 28209915
Seven NuMA isoforms generated by alternative splicing were categorized into 3 groups: long, middle and short. Both exons 15 and 16 in long NuMA were "hotspot" for alternative splicing. Lower expression of short NuMA was observed in cancer cells compared with nonneoplastic controls. PMID: 25451259
Chimeric proteins constructed by fusion of LANA of Kaposi's sarcoma-associated herpesvirus with the NuMA could bind with ori-P and enhance replication of an ori-P-containing plasmid. PMID: 27829174
findings reveal a direct physical link between two important regulators of mitotic progression and demonstrate the critical role of the NuMA-Astrin interaction for accurate cell division. PMID: 27462074
Aurora-A governs the dynamic exchange between the cytoplasmic and the spindle pole-localized pools of NuMA. Aurora-A phosphorylates directly the C terminus of NuMA on three Ser residues, of which Ser1969 determines the dynamic behavior and the spindle orientation functions of NuMA. PMID: 26832443
Suppressor APC domain containing 2 negatively regulates the localization of LGN at the cell cortex, likely by competing with NuMA for its binding PMID: 26766442
Letter: risk factors for false positive results when using urinary NMP22 as biomarker for early detection of bladder cancer. PMID: 24976592
Results show that at low grade of disease, NMP22 test provided a significantly higher sensitivity for the detection of recurrent urothelial carcinoma of the bladder compared to voided urine cytology specimens. PMID: 25488052
NuMA interacts with phosphoinositides and links the mitotic spindle with the plasma membrane.During anaphase correct NuMA localization is mediated by direct membrane phospholipid binding. PMID: 24996901
Retinoblastoma protein (pRB) have a novel function in regulating the mitotic function of NuMA and spindle organization, which are required for proper cell cycle progression. PMID: 24350565
Study finds that frictional forces increase nonlinearly with microtubule-associated proteins (MAP) velocity across microtubules and depend on filament polarity, with NuMA's friction being lower when moving toward minus ends, EB1's lower toward plus ends, and PRC1's exhibiting no directional preference. PMID: 24725408
The mitosis-dependent dynamic SUMO-1 modification of NuMA might contribute to NuMA-mediated formation and maintenance of mitotic spindle poles during mitosis. PMID: 24309115
ectopic expression of NuMA can manipulate endogenous p53 and p21 transcriptional expression during interphase. PMID: 23828576
ectopic expression of BRAP2 inhibits nuclear localization of HMG20A and NuMA1, and prevents nuclear envelope accumulation of SYNE2. PMID: 23707952
Hepatocyte Par1b defines lumen position in concert with the position of the astral microtubule anchoring complex LGN-NuMA to yield the distinct epithelial division phenotypes. PMID: 24165937
NuMA phosphorylation by CDK1 couples mitotic progression with cortical dynein function. PMID: 23921553
Phosphorylation of NuMA by aurora-A is important for cell survival. PMID: 23097092
Data indicate that dynein- and astral microtubule-mediated transport of Galphai/LGN/nuclear mitotic apparatus (NuMA) complex from cell cortex to spindle poles. PMID: 23389635
nuclear matrix protein 22 (nuclear mitotic apparatus protein, NuMA) has a role in upper tract urothelial tumors. PMID: 21865670
NuMA is required for the recruitment of cyclin-dependent kinase 8, a component of the Mediator complex and a promoter of p53-mediated p21 gene function. PMID: 23589328
Studies indicate that the Inscuteable (Insc)and NuMA are mutually exclusive interactors of LGN. PMID: 22977735
Numa regulates spindle assemby in conjunction with Eg5. PMID: 23368718
NuMA expression was upregulated in tumours, with a significant association with disease stage in mucinous EOC subtypes, lymph node involvement and patient age PMID: 22719996
Low NUMA1 is associtated with glioblastoma. PMID: 22619067
Without functional NuMA, microtubules lose connection to meiosis I spindle poles, resulting in highly disorganized early spindle assembly. PMID: 22552228
During apoptotic rearrangement of interchromatin granule clusters, the nuclear matrix (NuMa rearrangement) and chromatin are closely associated. This process occurs in defined stages and depends on the activity of protein phosphatases, caspases and CAD. PMID: 22023725
The levels of urinary NMP22 and CK18 in the patients with transitional cell carcinoma of the bladder were significantly higher than those in the non-transitional cell carcinoma of the bladder. PMID: 19615282
Accurate distribution of NuMA is important for oocyte maturation, zygote and embryo development in humans. Proper assembly of NuMA is likely necessary for bipolar spindle organization and human oocyte developmental competence. PMID: 21297155
NuMA is expressed in interphase nuclei of fibroblasts and oocytes. PMID: 21406448
Phenotype onset is correlated with NuMA-RARalpha copy number; mice with higher copy number developing disease later than those with lower copy number. PMID: 21255834
These results suggest that NuMA may provide structural support in the interphase nucleus by contributing to the organization of chromatin. PMID: 20467816
Ric-8A and Gi alpha recruit LGN, NuMA, and dynein to the cell cortex to help orient the mitotic spindle. PMID: 20479129
Data suggest that pADPr provides a dynamic cross-linking function at spindle poles by extending from covalent modification sites on PARP-5a and NuMA and binding noncovalently to NuMA and that this function helps promote assembly of exactly two poles. PMID: 19759176
A domain within the C-terminal tail of NuMA interacts with tubulin and induces bundling and stabilisation of microtubules and leads to formation of abnormal mitotic spindles. PMID: 11956313
NuMA is cleaved differently in Jurkat T and HeLa cells, suggesting that different sets of caspases are activated in these cell lines. The normal diffuse intranuclear distribution of NuMA changed during apoptosis. PMID: 12508117
role in development of myelodi leukemia with promyelocytic features PMID: 14737102
Proteins and open reading frames with a NuMA C terminus distal portion like region were found in a diverse set of vertebrate species including mammals, birds, amphibia, and early teleost fish. PMID: 15388855
Multiple mechanisms regulate NUMA1 dynamics at spindle poles. PMID: 15561764
concluded that variations in the NuMA gene are likely responsible for the observed increased breast cancer risk PMID: 15684076
NuMA plays diverse important roles in vertebrate cells [review] PMID: 16146802
NuMA has a role in mammary epithelial differentiation by influencing the organization of chromatin. PMID: 17108325
point to the Rae1-NuMA interaction as a critical element for normal spindle formation in mitosis PMID: 17172455

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Subcellular Location

Nucleus. Nucleus, nucleoplasm. Nucleus matrix. Chromosome. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cell cortex. Cell membrane; Lipid-anchor; Cytoplasmic side. Lateral cell membrane.; [Isoform 3]: Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.; [Isoform 4]: Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.

Database Links

HGNC: 8059

OMIM: 164009

KEGG: hsa:4926

STRING: 9606.ENSP00000377298

UniGene: Hs.325978

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Code	CSB-RA182340A0HU
Size	US$210
	Order now
Image	Overlay Peak curve showing Hela cells stained with CSB-RA182340A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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NUMA1 Recombinant Monoclonal Antibody

Product Details

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Target Background