Code | CSB-RA182340A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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FC | 1:50-1:200 |
The NUMA1 recombinant monoclonal antibody is generated through in vitro processes using synthetic genes. This methodology involves the retrieval of NUMA1 antibody genes from B cells sourced from immunoreactive rabbits, followed by their amplification and cloning into appropriate phage vectors. These vectors are then introduced into mammalian cell lines, enabling the production of functional antibodies in substantial quantities. Subsequently, the NUMA1 recombinant monoclonal antibody is purified from the culture supernatant of the transfected cell lines through affinity chromatography. Its functionality has been tested in ELISA and FC applications to react with human NUMA1 protein.
NUMA1 is a multifunctional protein primarily known for its critical role in organizing and stabilizing the mitotic spindle during cell division. Proper spindle assembly and chromosome segregation are essential for the accurate transmission of genetic material to daughter cells, and NUMA1 plays a central role in these processes.
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