Nox4 Antibody

Code CSB-PA015961LA01RA
Size US$299
  • Western Blot
    Positive WB detected in: U251 whole cell lysate, SH-SY5Y whole cell lysate
    All lanes: Nox4 antibody at 3.3µg/ml
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 67 kDa
    Observed band size: 67 kDa
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Product Details

Full Product Name Rabbit anti-Rattus norvegicus (Rat) Nox4 Polyclonal antibody
Uniprot No. Q924V1
Target Names Nox4
Alternative Names
Nox4 antibody; Kox antibody; NADPH oxidase 4 antibody; EC 1.6.3.- antibody; Kidney oxidase-1 antibody; KOX-1 antibody; Kidney superoxide-producing NADPH oxidase antibody
Raised in Rabbit
Species Reactivity Rat, Human
Immunogen Recombinant Rat NADPH oxidase 4 protein (210-424AA)
Immunogen Species Rattus norvegicus (Rat)
Conjugate Non-conjugated

The Nox4 Antibody (Product code: CSB-PA015961LA01RA) is Non-conjugated. For Nox4 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA015961LB01RA Nox4 Antibody, HRP conjugated ELISA
FITC CSB-PA015961LC01RA Nox4 Antibody, FITC conjugated
Biotin CSB-PA015961LD01RA Nox4 Antibody, Biotin conjugated ELISA
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.


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Applications : Immunofluorescence staining

Sample type: cell

Review: The in vivo suppressive effect of miR-148a on NOX4 was verified using immunofluorescence staining.

By Anonymous

Target Background

Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB.
Gene References into Functions
  1. chronic intermittent hypoxia-induced elevation of blood pressure is at least partially mediated via the Nox4-induced ROS/RhoA/ROCK pathway PMID: 28078487
  2. This study, for the first time, demonstrates that Nox4 is an oxygen-sensing enzyme and a main ROS source in NP cells. Nox4-dependent ROS are genotoxic and a potent trigger of NP cell senescence. Nox4 is a potential therapeutic target for disc cell senescence and IDD. PMID: 29147462
  3. The results demonstrate that the heightened sensitivity of the brain to ischemic damage is due to an organ-specific role of NOX4 in blood-brain barrier endothelial cells and neurons. PMID: 29087944
  4. Results indicate that intermedin exerts anti-oxidant effects by inhibition of Nox4, and the effect can be mediated by cAMP-PKA pathway in unilateral ureteral obstruction induced renal fibrosis. PMID: 28805491
  5. CYLD contributes to the transdifferentiation of adventitial fibroblasts via deubiquitinating Nox4 and may play a role in vascular remodeling. PMID: 28751569
  6. Oxygen-glucose deprivation/reoxygenation increased MEG3 and NOX4 expression in rat brain microvascular endothelial cells. PMID: 28634073
  7. increased mTAL luminal flow results in increases in intracellular and mitochondrial H2O2, which are dependent on the presence of Nox4, and that p67phox/Nox2 accounts solely for increases in O2 (.-) production PMID: 27279484
  8. Xbp1s plays an important role in NOX4-triggered cardiomyocyte hypertrophy via activating its downstream effector RIPK1, which may prove significant for the development of future therapeutic strategies. PMID: 27929749
  9. Nox4 activation in Vacular Smooth Muscle Cells contributes to actin depolymerization after hypoxia, which could be the underlying mechanism for myogenic tone impairment following ischemia/reperfusion. PMID: 27558234
  10. Upregulation of Nox4 is an important mechanism of cellular and mitochondrial oxidative stress leading to apoptotic cell death in cardiac myocytes following chronic beta- agonist stimulation. PMID: 26631573
  11. Luminal flow in thick ascending limbs leads to Nox4 recruitment to the nuclear region of thick ascending limb cells and an increase in O2 production. PMID: 27033446
  12. Acute restraint stress exacerbates the contractile hyper-reactivity in diabetic carotid arteries by enhancing Nox4 functionality. PMID: 26387612
  13. Hsp70 and CHIP mediates the ubiquitination and proteasomal degradation of Nox4 as part of the antioxidative effect of Losartan in spontaneous hypertensive rats. PMID: 26279425
  14. the reduced renal injury and attenuated blood pressure response to high salt in the SS(Nox4-/-) rat could be the result of multiple pathways by the knock out of Nox4. PMID: 26644237
  15. Glycated albumin increases the permissiveness of proximal tubular cells to fibrosis and apoptosis in vitro by triggering a pathway that involves Nox4. PMID: 25681565
  16. Results suggest that Nox4-mediated reactive oxygen species generation likely plays important role in fate determination and differentiation of neural crest stem cells. PMID: 25410908
  17. siNOX4 transfection markedly reduced rhBMP4-induced elevation of TRPC1 and 6 proteins. PMID: 25203114
  18. this study demonstrated a previously unrecognized protective role of Nox4, a ROS-generating enzyme and the major Nox isoform in CMECs, against H/R injury by inhibiting apoptosis and promoting migration and angiogenesis PMID: 24480752
  19. RhoA/ROCK is upstream of Poldip2-dependent Nox4 regulation and ROS production and induces redox signaling of kidney myofibroblast activation and may have broader implications in the pathophysiology of renal fibrosis. PMID: 24872317
  20. Nox4 is prominently expressed in the adventitia and contributes to altered fibroblast behavior, hypertensive vascular remodeling, and development of pulmonary hypertension. PMID: 24947524
  21. NOX4 activation is a decisive component in hepatic ischemia-reperfusion induced microcirculatory dysfunction. PMID: 24636100
  22. hyperglycemia could increase albumin permeability across the podocyte filtration barrier via Nox4-dependent PKGIalpha dimerization PMID: 24041960
  23. Data show that hypercholesterolemia affects myocardial microRNA expression, and by down-regulating microRNA-25 increases NOX4 expression and consequently oxidative/nitrative stress in the heart. PMID: 23722270
  24. Atrial fibroblasts show greater fibrotic and oxidative responses to TGF-beta1 than ventricular fibroblasts and suggest role for Nox4. PMID: 23884197
  25. Increased 8-OHdG concentration in the culture medium and higher expression of Nox-1, Nox-4, and p22(phox) mRNAs (3-6-fold) were observed in cells treated with AGE3. PMID: 23225244
  26. these results indicate that IL-1beta-dependent activation of PKCdelta is modulated by NOX4-derived ROS. PMID: 23022406
  27. the thyroid gland of females is exposed to higher oxidative stress levels due both to increased reactive oxygen species (ROS) production through NOX4, and decreased ROS degradation PMID: 23033809
  28. Nox4D is a nuclear-localized and functionally active splice variant of Nox4 that may have important pathophysiologic effects through modulation of nuclear signaling and DNA damage in vascular cells. PMID: 23393389
  29. Phenylephrine increased Nox4 protein expression and cardiomyocyte hypertrophy in a dose-dependent manner in cultured neonatal rat heart cells. PMID: 23271793
  30. Telmisartan downregulates myocardial expression of Nox4 in type 2 diabetic rats. PMID: 22873349
  31. NOX4 is the main catalytic isoform of NADPH oxidase that contributes to oxygen production in the thick ascending limb of kidney. PMID: 22875785
  32. Study shows that testosterone induces vascular smooth muscle cells (VSMCs) migration via NADPH oxidase (Nox1 and Nox4 mRNA levels and p47phox)-derived reactive oxygen species (ROS) and c-Src-dependent pathways. PMID: 22566500
  33. Abnormal expression of NADPH oxidase plays an important role in progression of hypertensive renal damage in spontaneously hypertensive rats. PMID: 21646815
  34. In the aorta, Nox4 expression was increased by three-fold in obese rats. PMID: 22195989
  35. This study provides strong evidence that Nox4 is an important source of reactive oxygen species (ROS) in the left ventricle and that Nox4-derived ROS contribute to cardiomyopathy at early stages of type 1 diabetes PMID: 22031600
  36. Intracellular reactive oxygen species formation partly through an increasing NOX4 activity in response to high pacing frequency is associated with an increased atrial natriuretic peptide secretion. PMID: 22063193
  37. IGF-I induces an increase in expression of Nox4 in cultured renal proximal tubular epithelial cells. PMID: 21940672
  38. Expression of Nox4 was increased in vascular smooth muscle cells from SHR. Nox4,alone or in association,may be involved in NAD(P)H oxidase-mediated ROS production. PMID: 21419746
  39. High NOX4 expression is associated with colon cancers. PMID: 20715105
  40. Our results indicate that miR-25 as an endogenous gene silencing factor importantly contributes to the regulation of NOX4 gene expression in diabetic nephropathy. PMID: 21071935
  41. Bilirubin and biliverdin protect rodents against diabetic nephropathy by downregulating NAD(P)H oxidase. PMID: 20686447
  42. Upregulation of Nox4 by hypertrophic stimuli and aging induces oxidative stress, apoptosis and left ventricular dysfunction, via mitochondrial insufficiency caused by increased O(2)(-) production and cysteine oxidation in mitochondrial proteins. PMID: 20185797
  43. Translocation of Hsp70 to proximal tubule membranes in SHRLos might exert a cytoprotective effect by down-regulation of NADPH subunits Nox4. PMID: 20016382
  44. NADPH oxidase-derived reactive oxygen species is one of critical components of a signal transduction pathway that links puromycin aminonucleoside nephrosis to TRPC6-mediated Ca(2+) signaling. PMID: 19910702
  45. Nox4-based NAD(P)H oxidase and generation of reactive oxygen species mediate the effect of ANG II on Akt/PKB activation and protein synthesis in glomerular mesangial cells PMID: 12842860
  46. Nox4 co-localizes with vinculin in focal adhesions in vascular smooth muscle cells. Its role may be correlated with its compartmentalization in focal adhesions. PMID: 14670934
  47. cardiac fibroblasts express Nox4/p22 phox-containing oxidant generating complex activated by H(2)O(2) PMID: 15848200
  48. role for Nox4 as the major source of ROS in the kidneys during early stages of diabetes PMID: 16135519
  49. Nox4-derived reactive oxygen species are critical to the maintenance of the differentiated phenotype of vascular smooth muscle cells. PMID: 17082491
  50. blood pressure and angiotensin II type 1 receptor activation are involved in the up-regulation of Nox1 and Nox4 expression, which could contribute to vascular injury during chronic hypertension PMID: 17283869

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Subcellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cell junction, focal adhesion. Note=May localize to plasma membrane and focal adhesions.
Tissue Specificity Expressed in vascular smooth muscle.
Database Links

KEGG: rno:85431

STRING: 10116.ENSRNOP00000018990

UniGene: Rn.14744

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